LOCUS       sequence-274239-       11729 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian Expression, Lentiviral. Expression of human 
            alpha-actinin-1-GFP fusion protein .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11729)
  AUTHORS   Park JS, Burckhardt CJ, Lazcano R, Solis LM, Isogai T, Li L, Chen 
            CS, Gao B, Minna JD, Bachoo R, DeBerardinis RJ, Danuser G
  TITLE     Mechanical regulation of glycolysis via cytoskeleton architecture.
  JOURNAL   Nature. 2020 Feb 12. pii: 10.1038/s41586-020-1998-1. doi: 
            10.1038/s41586-020-1998-1.
  PUBMED    32051585
REFERENCE   2  (bases 1 to 11729)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2020 Feb 12. pii: 10.1038/s41586-020-1998-1. doi: 
            10.1038/s41586-020-1998-1."
COMMENT     Sequence Label: pLVX neo ACTN1-GFP
FEATURES             Location/Qualifiers
     source          1..11729
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     505..529
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      3360..3369
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3366..3368,3369..3371,3372..4085)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3366..3368,3369..3371,3372..4082)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3411..3432)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3672..3691)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4019..4040
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    join(4103..4677,4678..4680,4681..4689)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4270..4287)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4497..4516
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4690..5484
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MAEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4744..4763)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5354..5373
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    5498..6086
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5551..5571)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5969..5980)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6293..6926
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7054..7070)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7054..7070)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7067..7089)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7078..7094
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7102..7108,7109..7126,7127..7132))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7147..7168
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7285..7302)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7456..8044)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7536..7555)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8215..9006,9007..9075))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8838..8857
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9076..9180)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    9228..9362
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9265..9284)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9319..9338
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             9530..10163
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    10210..10335
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10832..11065
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11250..11294
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    11557..11672
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcg cctggagacg
      541 ccatccacgc tgttttgacc tccatagaag acaccgactc tactagagga tctatttccg
      601 gtgaattcct cgagctcaag cttcgcacca tcatggacca ttatgattct cagcaaacca
      661 acgattacat gcagccagaa gaggactggg accgggacct gctcctggac ccggcctggg
      721 agaagcagca gagaaagaca ttcacggcat ggtgtaactc ccacctccgg aaggcgggga
      781 cacagatcga gaacatcgaa gaggacttcc gggatggcct gaagctcatg ctgctgctgg
      841 aggtcatctc aggtgaacgc ttggccaagc cagagcgagg caagatgaga gtgcacaaga
      901 tctccaacgt caacaaggcc ctggatttca tagccagcaa aggcgtcaaa ctggtgtcca
      961 tcggagccga agaaatcgtg gatgggaatg tgaagatgac cctgggcatg atctggacca
     1021 tcatcctgcg ctttgccatc caggacatct ccgtggaaga gacttcagcc aaggaagggc
     1081 tgctcctgtg gtgtcagaga aagacagccc cttacaaaaa tgtcaacatc cagaacttcc
     1141 acataagctg gaaggatggc ctcggcttct gtgctttgat ccaccgacac cggcccgagc
     1201 tgattgacta cgggaagctg cggaaggatg atccactcac aaatctgaat acggcttttg
     1261 acgtggcaga gaagtacctg gacatcccca agatgctgga tgccgaagac atcgttggaa
     1321 ctgcccgacc ggatgagaaa gccatcatga cttacgtgtc tagcttctac cacgccttct
     1381 ctggagccca gaaggcggag acagcagcca atcgcatctg caaggtgttg gccgtcaacc
     1441 aggagaacga gcagcttatg gaagactacg agaagctggc cagtgatctg ttggagtgga
     1501 tccgccgcac aatcccgtgg ctggagaacc gggtgcccga gaacaccatg catgccatgc
     1561 aacagaagct ggaggacttc cgggactacc ggcgcctgca caagccgccc aaggtgcagg
     1621 agaagtgcca gctggagatc aacttcaaca cgctgcagac caagctgcgg ctcagcaacc
     1681 ggcctgcctt catgccctct gagggcagga tggtctcgga catcaacaat gcctggggct
     1741 gcctggagca ggtggagaag ggctatgagg agtggttgct gaatgagatc cggaggctgg
     1801 agcgactgga ccacctggca gagaagttcc ggcagaaggc ctccatccac gaggcctgga
     1861 ctgacggcaa agaggccatg ctgcgacaga aggactatga gaccgccacc ctctcggaga
     1921 tcaaggcctt gctcaagaag catgaggcct tcgagagtga cctggctgcc caccaggacc
     1981 gtgtggagca gattgccgcc atcgcacagg agctcaatga gctggactat tatgactcac
     2041 ccagtgtcaa cgcccgttgc caaaagatct gtgaccagtg ggacaatctg ggggccctaa
     2101 ctcagaagcg aagggaagct ctggagcgga ccgagaaact gctggagacc attgaccagc
     2161 tgtacttgga gtatgccaag cgggctgcac ccttcaacaa ctggatggag ggggccatgg
     2221 aggacctgca ggacaccttc attgtgcaca ccattgagga gatccaggga ctgaccacag
     2281 cccatgagca gttcaaggcc accctccctg atgccgacaa ggagcgcctg gccatcctgg
     2341 gcatccacaa tgaggtgtcc aagattgtcc agacctacca cgtcaatatg gcgggcacca
     2401 acccctacac aaccatcacg cctcaggaga tcaatggcaa atgggaccac gtgcggcagc
     2461 tggtgcctcg gagggaccaa gctctgacgg aggagcatgc ccgacagcag cacaatgaga
     2521 ggctacgcaa gcagtttgga gcccaggcca atgtcatcgg gccctggatc cagaccaaga
     2581 tggaggagat cgggaggatc tccattgaga tgcatgggac cctggaggac cagctcagcc
     2641 acctgcggca gtatgagaag agcatcgtca actacaagcc aaagattgat cagctggagg
     2701 gcgaccacca gctcatccag gaggcgctca tcttcgacaa caagcacacc aactacacca
     2761 tggagcacat ccgtgtgggc tgggagcagc tgctcaccac catcgccagg accatcaatg
     2821 aggtagagaa ccagatcctg acccgggatg ccaagggcat cagccaggag cagatgaatg
     2881 agttccgggc ctccttcaac cactttgacc gggatcactc cggcacactg ggtcccgagg
     2941 agttcaaagc ctgcctcatc agcttgggtt atgatattgg caacgacccc cagggagaag
     3001 cagaatttgc ccgcatcatg agcattgtgg accccaaccg cctgggggta gtgacattcc
     3061 aggccttcat tgacttcatg tcccgcgaga cagccgacac agatacagca gaccaagtca
     3121 tggcttcctt caagatcctg gctggggaca agaactacat taccatggac gagctgcgcc
     3181 gcgagctgcc acccgaccag gctgagtact gcatcgcgcg gatggccccc tacaccggcc
     3241 ccgactccgt gccaggtgct ctggactaca tgtccttctc cacggcgctg tacggcgaga
     3301 gtgacctcaa gcttcgaatt ctgcagtcga cggtaccgcg ggcccgggat ccaccggtcg
     3361 ccaccatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc
     3421 tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca
     3481 cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc
     3541 ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca
     3601 tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca
     3661 tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca
     3721 ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg
     3781 ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga
     3841 agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc
     3901 tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca
     3961 accactacct gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca
     4021 tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca
     4081 agtaaagcgg ccgcggatcc cgcccctctc cctccccccc ccctaacgtt actggccgaa
     4141 gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt attttccacc atattgccgt
     4201 cttttggcaa tgtgagggcc cggaaacctg gccctgtctt cttgacgagc attcctaggg
     4261 gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc
     4321 ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg cagcggaacc
     4381 ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg tgtataagat acacctgcaa
     4441 aggcggcaca accccagtgc cacgttgtga gttggatagt tgtggaaaga gtcaaatggc
     4501 tctcctcaag cgtattcaac aaggggctga aggatgccca gaaggtaccc cattgtatgg
     4561 gatctgatct ggggcctcgg tgcacatgct ttacatgtgt ttagtcgagg ttaaaaaaac
     4621 gtctaggccc cccgaaccac ggggacgtgg ttttcctttg aaaaacacga tgataagctt
     4681 gccacaacca tggctgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag
     4741 aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc
     4801 cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg
     4861 aatgaactgc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc
     4921 gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg
     4981 ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct
     5041 gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg
     5101 aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat
     5161 ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc
     5221 atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg
     5281 gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc
     5341 tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct
     5401 gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat
     5461 cgccttcttg acgagttctt ctgaacgcgt ctggaacaat caacctctgg attacaaaat
     5521 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc
     5581 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt
     5641 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg
     5701 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg
     5761 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc
     5821 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt
     5881 gttgtcgggg aagctgacgt cctttccatg gctgctcgcc tgtgttgcca cctggattct
     5941 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg
     6001 cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg
     6061 gatctccctt tgggccgcct ccccgcctgg aattaattct gcagtcgaga cctagaaaaa
     6121 catggagcaa tcacaagtag caatacagca gctaccaatg ctgattgtgc ctggctagaa
     6181 gcacaagagg aggaggaggt gggttttcca gtcacacctc aggtaccttt aagaccaatg
     6241 acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagagggg actggaaggg
     6301 ctaattcact cccaacgaag acaagatatc cttgatctgt ggatctacca cacacaaggc
     6361 tacttccctg attagcagaa ctacacacca gggccagggg tcagatatcc actgaccttt
     6421 ggatggtgct acaagctagt accagttgag ccagataagg tagaagaggc caataaagga
     6481 gagaacacca gcttgttaca ccctgtgagc ctgcatggga tggatgaccc ggagagagaa
     6541 gtgttagagt ggaggtttga cagccgccta gcatttcatc acgtggcccg agagctgcat
     6601 ccggagtact tcaagaactg ctgatatcga gcttgctaca agggactttc cgctggggac
     6661 tttccaggga ggcgtggcct gggcgggact ggggagtggc gagccctcag atcctgcata
     6721 taagcagctg ctttttgcct gtactgggtc tctctggtta gaccagatct gagcctggga
     6781 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     6841 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     6901 ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt
     6961 ataacttgca aagaaatgaa tatcagagag tgagaggcct tgacattgct agcgtttacc
     7021 gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
     7081 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
     7141 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     7201 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     7261 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     7321 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     7381 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     7441 cgcgttgctg gcgtttttcc ataggctccg cccccgtgac gagcatcaca aaaatcgacg
     7501 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     7561 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     7621 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     7681 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     7741 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     7801 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     7861 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     7921 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     7981 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     8041 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     8101 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     8161 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     8221 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     8281 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     8341 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     8401 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     8461 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     8521 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     8581 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     8641 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     8701 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     8761 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     8821 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     8881 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     8941 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     9001 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     9061 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     9121 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     9181 cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggagatcaac ttgtttattg
     9241 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     9301 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga
     9361 tcaactggat aactcaagct aaccaaaatc atcccaaact tcccacccca taccctatta
     9421 ccactgccaa ttacctgtgg tttcatttac tctaaacctg tgattcctct gaattatttt
     9481 cattttaaag aaattgtatt tgttaaatat gtactacaaa cttagtagtt ggaagggcta
     9541 attcactccc aaagaagaca agatatcctt gatctgtgga tctaccacac acaaggctac
     9601 ttccctgatt agcagaacta cacaccaggg ccaggggtca gatatccact gacctttgga
     9661 tggtgctaca agctagtacc agttgagcca gataaggtag aagaggccaa taaaggagag
     9721 aacaccagct tgttacaccc tgtgagcctg catgggatgg atgacccgga gagagaagtg
     9781 ttagagtgga ggtttgacag ccgcctagca tttcatcacg tggcccgaga gctgcatccg
     9841 gagtacttca agaactgctg atatcgagct tgctacaagg gactttccgc tggggacttt
     9901 ccagggaggc gtggcctggg cgggactggg gagtggcgag ccctcagatc ctgcatataa
     9961 gcagctgctt tttgcctgta ctgggtctct ctggttagac cagatctgag cctgggagct
    10021 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
    10081 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
    10141 gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa
    10201 ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag
    10261 gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat
    10321 gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg
    10381 ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag
    10441 ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata
    10501 ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat
    10561 acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct
    10621 ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccggc
    10681 cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt gaattatata
    10741 aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag
    10801 tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag
    10861 cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat
    10921 tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc
    10981 tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa
    11041 gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca
    11101 ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag atttggaatc
    11161 acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct
    11221 taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata
    11281 aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat
    11341 tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag
    11401 tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga
    11461 ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac agagacagat
    11521 ccattcgatt agtgaacgga tctcgacggt atcgccttta aaagaaaagg ggggattggg
    11581 gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa
    11641 ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat
    11701 ccagtttatc gataagcttg ggagttccg
//
