LOCUS       sequence-27424-e         602 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 602)
  AUTHORS   Wollert T, Wunder C, Lippincott-Schwartz J, Hurley JH
  TITLE     Membrane scission by the ESCRT-III complex.
  JOURNAL   Nature. 2009 Mar 12. 458(7235):172-7.
  PUBMED    19234443
REFERENCE   2  (bases 1 to 602)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2009 Mar 12. 458(7235):172-7."
COMMENT     Sequence Label: pMBP-HIS2-Vps20FL
FEATURES             Location/Qualifiers
     source          1..602
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             13..35
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             55..72
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ctctagaata attttgttta actttaagaa ggagatatac atatgtcgta ctaccatcac
       61 catcaccatc acgattacat gatcgaagaa ggtaaactgg taatctggat taacggcgat
      121 aaaggctata acggtctcgc tgaagtcggt aagaaattcg agaaagatac cggaattaaa
      181 gtcaccgttg agcatccgga taaactggaa gagaaattcc cacaggttgc ggcaactggc
      241 gatggccctg acattatctt ctgggcacac gaccgctttg gtggctacgc tcaatctggc
      301 ctgttggctg aaatcacccc ggacaaagcg ttccaggaca agctgtatcc gtttacctgg
      361 gatgccgtac gttacaacgg caagctgatt gcttacccga tcgctgttga agcgttatcg
      421 ctgatttata acaaagatct gctgccgaac ccgccaaaaa cctgggaaga gatcccggcg
      481 ctggataaag aactgaaagc gaaaggtaag agcgcgctga tgttcaacct gcaagaaccg
      541 tacttcacct ggccgctgat tgctgctgac gggggttatg cgttcaagta tgaaaacggc
      601 aa
//
