LOCUS       sequence-274275-       11755 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11755)
  TITLE     Bjorkman lab plasmids
REFERENCE   2  (bases 1 to 11755)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAcGP67A - murine TfR
FEATURES             Location/Qualifiers
     source          1..11755
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     87..106
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     300..322
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     314..331
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     315..331
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_recomb     3504..4331
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"
     CDS             3504..3628
                     /codon_start=3
                     /product="baculovirus late expression factor 2"
                     /label=lef2
                     /translation="HLLIKSQDVYKPPNCQKMKTVDKLCPFAGNCKGLNPICNY"
     CDS             complement(3666..4271)
                     /codon_start=1
                     /product="baculovirus ORF603 protein"
                     /label=ORF603
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNGV
                     IELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIHPI
                     KKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKIILSQ
                     INCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR"
     promoter        4335..4426
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     4351..4368
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     sig_peptide     4523..4582
                     /codon_start=1
                     /gene="Autographa californica gp64"
                     /product="signal sequence from the baculovirus envelope 
                     glycoprotein GP64"
                     /label=GP64 signal sequence
                     /translation="MVSAIVLYVLLAAAAHSAFA"
     CDS             4592..4609
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             4619..4630
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     misc_recomb     7097..7802
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     3'UTR           7185..7557
                     /label=polyhedrin 3' UTR
                     /note="3' UTR from the Autographa californica 
                     nucleopolyhedrovirus polyhedrin gene (Suzuki et al., 2006)"
     CDS             complement(7214..7802)
                     /codon_start=2
                     /product="baculovirus capsid-associated protein"
                     /label=ORF1629
                     /note="required for viral replication"
                     /translation="IPKSSTTNLIADVLADTINRRRVAMAKSSSEATSNDEGWDDDDNR
                     PNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLLQNKTQTNIDKAR
                     LLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIFKFRYKDAENHLIF
                     ALTYHPKDYKFNELLKYVQQLSVNQQRTESSA"
     CDS             complement(8236..8253)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(9470..9486)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9470..9486)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9483..9505)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9494..9510
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9518..9524,9525..9542,9543..9548))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9563..9584
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9701..9718)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9872..10460)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9952..9971)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10631..11422,11423..11491))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11254..11273
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11492..11596)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     11664..11682
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(11720..11742)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg
       61 cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca
      121 tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgccattc
      181 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg
      241 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc
      301 ccagtcacga cgttgtaaaa cgacggccag tgccaagctt ggctcgtaaa gcgagttgaa
      361 ggatcatatt tagttgcgtt tatgagataa gattgaaagc acgtgtaaaa tgtttcccgc
      421 gcgttggcac aactatttac aatgcggcca agttataaaa gattctaatc tgatatgttt
      481 taaaacacct ttgcggcccg agttgtttgc gtacgtgact agcgaagaag atatgtggac
      541 cgcagaacag atagtaaaac aaaaccctag tattggagca ataatcgatt taaccaacac
      601 gtctaaatat tatgatggtg tgcatttttt gcgggcgggc ctgttataca aaaaaattca
      661 agtacctggc cagactttgc cgcctgaaag catagttcaa gaatttattg acacggtaaa
      721 agaatttaca gaaaagtgtc ccggcatgtt ggtgggcgtg cactgcacac acggtattaa
      781 tcgcaccggt tacatggtgt gcagatattt aatgcacacc ctgggtattg cgccgcagga
      841 agccatagat agattcgaaa aagccagagg tcacaaaatt gaaagacaaa attacgttca
      901 agatttatta atttaattaa tattatttgc attctttaac aaatacttta tcctattttc
      961 aaattgttgc gcttcttcca gcgaaccaaa actatgcttc gcttgctccg tttagcttgt
     1021 agccgatcag tggcgttgtt ccaatcgacg gtaggattag gccggatatt ctccaccaca
     1081 atgttggcaa cgttgatgtt acgtttatgc ttttggtttt ccacgtacgt cttttggccg
     1141 gtaatagccg taaacgtagt gccgtcgcgc gtcacgcaca acaccggatg tttgcgcttg
     1201 tccgcggggt attgaaccgc gcgatccgac aaatccacca ctttggcaac taaatcggtg
     1261 acctgcgcgt cttttttctg cattatttcg tctttctttt gcatggtttc ctggaagccg
     1321 gtgtacatgc ggtttagatc agtcatgacg cgcgtgacct gcaaatcttt ggcctcgatc
     1381 tgcttgtcct tgatggcaac gatgcgttca ataaactctt gttttttaac aagttcctcg
     1441 gttttttgcg ccaccaccgc ttgcagcgcg tttgtgtgct cggtgaatgt cgcaatcagc
     1501 ttagtcacca actgtttgct ctcctcctcc cgttgtttga tcgcgggatc gtacttgccg
     1561 gtgcagagca cttgaggaat tacttcttct aaaagccatt cttgtaattc tatggcgtaa
     1621 ggcaatttgg acttcataat cagctgaatc acgccggatt tagtaatgag cactgtatgc
     1681 ggctgcaaat acagcgggtc gccccttttc acgacgctgt tagaggtagg gcccccattt
     1741 tggatggtct gctcaaataa cgatttgtat ttattgtcta catgaacacg tatagcttta
     1801 tcacaaactg tatattttaa actgttagcg acgtccttgg ccacgaaccg gacctgttgg
     1861 tcgcgctcta gcacgtaccg caggttgaac gtatcttctc caaatttaaa ttctccaatt
     1921 ttaacgcgag ccattttgat acacgtgtgt cgattttgca acaactattg ttttttaacg
     1981 caaactaaac ttattgtggt aagcaataat taaatatggg ggaacatgcg ccgctacaac
     2041 actcgtcgtt atgaacgcag acggcgccgg tctcggcgca agcggctaaa acgtgttgcg
     2101 cgttcaacgc ggcaaacatc gcaaaagcca atagtacagt tttgatttgc atattaacgg
     2161 cgatttttta aattatctta tttaataaat agttatgacg cctacaactc cccgcccgcg
     2221 ttgactcgct gcacctcgag cagttcgttg acgccttcct ccgtgtggcc gaacacgtcg
     2281 agcgggtggt cgatgaccag cggcgtgccg cacgcgacgc acaagtatct gtacaccgaa
     2341 tgatcgtcgg gcgaaggcac gtcggcctcc aagtggcaat attggcaaat tcgaaaatat
     2401 atacagttgg gttgtttgcg catatctatc gtggcgttgg gcatgtacgt ccgaacgttg
     2461 atttgcatgc aagccgaaat taaatcattg cgattagtgc gattaaaacg ttgtacatcc
     2521 tcgcttttaa tcatgccgtc gattaaatcg cgcaatcgag tcaagtgatc aaagtgtgga
     2581 ataatgtttt ctttgtattc ccgagtcaag cgcagcgcgt attttaacaa actagccatc
     2641 ttgtaagtta gtttcattta atgcaacttt atccaataat atattatgta tcgcacgtca
     2701 agaattaaca atgcgcccgt tgtcgcatct caacacgact atgatagaga tcaaataaag
     2761 cgcgaattaa atagcttgcg acgcaacgtg cacgatctgt gcacgcgttc cggcacgagc
     2821 tttgattgta ataagttttt acgaagcgat gacatgaccc ccgtagtgac aacgatcacg
     2881 cccaaaagaa ctgccgacta caaaattacc gagtatgtcg gtgacgttaa aactattaag
     2941 ccatccaatc gaccgttagt cgaatcagga ccgctggtgc gagaagccgc gaagtatggc
     3001 gaatgcatcg tataacgtgt ggagtccgct cattagagcg tcatgtttag acaagaaagc
     3061 tacatattta attgatcccg atgattttat tgataaattg accctaactc catacacggt
     3121 attctacaat ggcggggttt tggtcaaaat ttccggactg cgattgtaca tgctgttaac
     3181 ggctccgccc actattaatg aaattaaaaa ttccaatttt aaaaaacgca gcaagagaaa
     3241 catttgtatg aaagaatgcg tagaaggaaa gaaaaatgtc gtcgacatgc tgaacaacaa
     3301 gattaatatg cctccgtgta taaaaaaaat attgaacgat ttgaaagaaa acaatgtacc
     3361 gcgcggcggt atgtacagga agaggtttat actaaactgt tacattgcaa acgtggtttc
     3421 gtgtgccaag tgtgaaaacc gatgtttaat caaggctctg acgcatttct acaaccacga
     3481 ctccaagtgt gtgggtgaag tcatgcatct tttaatcaaa tcccaagatg tgtataaacc
     3541 accaaactgc caaaaaatga aaactgtcga caagctctgt ccgtttgctg gcaactgcaa
     3601 gggtctcaat cctatttgta attattgaat aataaaacaa ttataaatgt caaatttgtt
     3661 ttttattaac gatacaaacc aaacgcaaca agaacatttg tagtattatc tataattgaa
     3721 aacgcgtagt tataatcgct gaggtaatat ttaaaatcat tttcaaatga ttcacagtta
     3781 atttgcgaca atataatttt attttcacat aaactagacg ccttgtcgtc ttcttcttcg
     3841 tattccttct ctttttcatt tttctcctca taaaaattaa catagttatt atcgtatcca
     3901 tatatgtatc tatcgtatag agtaaatttt ttgttgtcat aaatatatat gtctttttta
     3961 atggggtgta tagtaccgct gcgcatagtt tttctgtaat ttacaacagt gctattttct
     4021 ggtagttctt cggagtgtgt tgctttaatt attaaattta tataatcaat gaatttggga
     4081 tcgtcggttt tgtacaatat gttgccggca tagtacgcag cttcttctag ttcaattaca
     4141 ccatttttta gcagcaccgg attaacataa ctttccaaaa tgttgtacga accgttaaac
     4201 aaaaacagtt cacctccctt ttctatacta ttgtctgcga gcagttgttt gttgttaaaa
     4261 ataacagcca ttgtaatgag acgcacaaac taatatcaca aactggaaat gtctatcaat
     4321 atatagttgc tgatatcatg gagataatta aaatgataac catctcgcaa ataaataagt
     4381 attttactgt tttcgtaaca gttttgtaat aaaaaaacct ataaatattc cggattattc
     4441 ataccgtccc accatcgggc gcggatctat gctactagta aatcagtcac accaaggctt
     4501 caataaggaa cacacaagca agatggtaag cgctattgtt ttatatgtgc ttttggcggc
     4561 ggcggcgcat tctgcctttg cggcggatcc gcatcatcat catcatcaca gcagcggcat
     4621 cgagggacgt ggtgaattcc gcttatattg ggcagacctc aaaacactgt tgtcagagaa
     4681 gttgaactcc atagagtttg ctgacaccat caagcagctg agccagaata catacactcc
     4741 tcgtgaggct ggatctcaaa aagatgaaag tcttgcctat tatattgaaa atcagttcca
     4801 tgaatttaaa ttcagcaaag tctggcgaga tgaacactat gtgaagattc aagtgaaaag
     4861 cagcattggt caaaacatgg tgaccatagt gcagtcaaat ggtaacttag acccagtgga
     4921 gtctcccgag ggttatgtgg cattcagtaa acctacagaa gtttctggta aactggtcca
     4981 tgctaatttt ggcactaaaa aggactttga agaactaagt tattctgtga atggatcttt
     5041 agtgattgtt agagcagggg aaattacttt tgcagaaaag gttgcaaatg cccaaagctt
     5101 taatgcaatt ggtgtcctca tatacatgga caagaataaa ttccccgttg ttgaggcaga
     5161 ccttgcactc tttggacatg ctcatctagg aactggtgat ccatacacac ctggctttcc
     5221 ttctttcaat catactcagt ttccgccatc tcagtcatca gggttgccta atatacctgt
     5281 gcaaacaatc tcaagagctg ctgcagaaaa gctatttgga aaaatggaag gaagctgtcc
     5341 tgctagatgg aacatagatt cttcatgtaa gctggaactt tcacagaatc aaaatgtgaa
     5401 gctcattgtg aaaaacgtac tgaaagaaag aagaatactt aacatctttg gagttattaa
     5461 aggttatgag gaaccagacc gttatgttgt agtaggagcc cagagagacg ctttgggtgc
     5521 tggtgttgcg gcgaagtcca gtgtgggaac aggtcttctg ttgaaacttg cccaagtatt
     5581 ctcagatatg atttcaaaag atggatttag acccagcaga agtataatct ttgccagctg
     5641 gactgcaggc gactttggag ctgttggtgc cactgagtgg ttggagggat acctttcatc
     5701 tttgcattta aaagctttca cttatattaa tttggataaa gttgtccttg gtactagtaa
     5761 cttcaaagtt tctgccagcc ccttattata tacacttatg ggaaagataa tgcaagatgt
     5821 aaagcatcca gttgatggaa aatctctata tagagacagc aattggatta gcaaagttga
     5881 gaaactttcc tttgacaatg ctgcatatcc tttccttgca tattctggaa tcccagcagt
     5941 ttctttttgt ttttgtgagg atgcagacta tccttatttg ggcactagat tggataccta
     6001 tgaggcattg actcagaaag ttcctcagct caaccaaatg gttcgtacag cagcggaagt
     6061 ggctggtcag ctcattatta aacttaccca tgacgttgaa ttgaacctgg actatgagat
     6121 gtataacagc aaactactgt catttatgaa ggatctgaac cagttcaaaa cagatatcag
     6181 ggatatgggt ctaagtctac agtggctgta ttccgctcgt ggagactact tccgtgctac
     6241 ttctagacta acaactgatt ttcataatgc tgagaaaaca aacagatttg tcatgaggga
     6301 aatcaatgat cgtattatga aagtggagta tcacttcctg tcgccctatg tatctccaag
     6361 agagtctcct ttccgacata tcttctgggg ctctggctct cacactctct cagctttagt
     6421 ggagaacttg aagcttcgtc aaaaaaatat tactgctttt aatgaaaccc tcttcagaaa
     6481 ccagttggcc ctggctactt ggactattca gggagtcgca aatgccctct ctggtgacat
     6541 ttggaatatt gacaatgagt tttaaatgta acatgcataa ttaaataaga gcagggtagt
     6601 ccggccgctg cagatctgat cctttcctgg gacccggcaa gaaccaaaaa ctcactctct
     6661 tcaaggaaat ccgtaatgtt aaacccgaca cgatgaagct tgtcgttgga tggaaaggaa
     6721 aagagttcta cagggaaact tggacccgct tcatggaaga cagcttcccc attgttaacg
     6781 accaagaagt gatggatgtt ttccttgttg tcaacatgcg tcccactaga cccaaccgtt
     6841 gttacaaatt cctggcccaa cacgctctgc gttgcgaccc cgactatgta cctcatgacg
     6901 tgattaggat cgtcgagcct tcatgggtgg gcagcaacaa cgagtaccgc atcagcctgg
     6961 ctaagaaggg cggcggctgc ccaataatga accttcactc tgagtacacc aactcgttcg
     7021 aacagttcat cgatcgtgtc atctgggaga acttctacaa gcccatcgtt tacatcggta
     7081 ccgactctgc tgaagaggag gaaattctcc ttgaagtttc cctggtgttc aaagtaaagg
     7141 agtttgcacc agacgcacct ctgttcactg gtccggcgta ttaaaacacg atacattgtt
     7201 attagtacat ttattaagcg ctagattctg tgcgttgttg atttacagac aattgttgta
     7261 cgtattttaa taattcatta aatttataat ctttagggtg gtatgttaga gcgaaaatca
     7321 aatgattttc agcgtcttta tatctgaatt taaatattaa atcctcaata gatttgtaaa
     7381 ataggtttcg attagtttca aacaagggtt gtttttccga accgatggct ggactatcta
     7441 atggattttc gctcaacgcc acaaaacttg ccaaatcttg tagcagcaat ctagctttgt
     7501 cgatattcgt ttgtgttttg ttttgtaata aaggttcgac gtcgttcaaa atattatgcg
     7561 cttttgtatt tctttcatca ctgtcgttag tgtacaattg actcgacgta aacacgttaa
     7621 ataaagcttg gacatattta acatcgggcg tgttagcttt attaggccga ttatcgtcgt
     7681 cgtcccaacc ctcgtcgtta gaagttgctt ccgaagacga ttttgccata gccacacgac
     7741 gcctattaat tgtgtcggct aacacgtccg cgatcaaatt tgtagttgag ctttttggaa
     7801 ttatttctga ttgcgggcgt ttttgggcgg gtttcaatct aactgtgccc gattttaatt
     7861 cagacaacac gttagaaagc gatggtgcag gcggtggtaa catttcagac ggcaaatcta
     7921 ctaatggcgg cggtggtgga gctgatgata aatctaccat cggtggaggc gcaggcgggg
     7981 ctggcggcgg aggcggaggc ggaggtggtg gcggtgatgc agacggcggt ttaggctcaa
     8041 atgtctcttt aggcaacaca gtcggcacct caactattgt actggtttcg ggcgccgttt
     8101 ttggtttgac cggtctgaga cgagtgcgat ttttttcgtt tctaatagct tccaacaatt
     8161 gttgtctgtc gtctaaaggt gcagcgggtt gaggttccgt cggcattggt ggagcgggcg
     8221 gcaattcaga catcgatggt ggtggtggtg gtggaggcgc tggaatgtta ggcacgggag
     8281 aaggtggtgg cggcggtgcc gccggtataa tttgttctgg tttagtttgt tcgcgcacga
     8341 ttgtgggcac cggcgcaggc gccgctggct gcacaacgga aggtcgtctg cttcgaggca
     8401 gcgcttgggg tggtggcaat tcaatattat aattggaata caaatcgtaa aaatctgcta
     8461 taagcattgt aatttcgcta tcgtttaccg tgccgatatt taacaaccgc tcaatgtaag
     8521 caattgtatt gtaaagagat tgtctcaagc tcgcacgccg ataacaagcc ttttcatttt
     8581 tactacagca ttgtagtggc gagacacttc gctgtcgtcg acgtacatgt atgctttgtt
     8641 gtcaaaaacg tcgttggcaa gctttaaaat atttaaaaga acatctctgt tcagcaccac
     8701 tgtgttgtcg taaatgttgt ttttgataat ttgcgcttcc gcagtatcga cacgttcaaa
     8761 aaattgatgc gcatcaattt tgttgttcct attattgaat aaataagatt gtacagattc
     8821 atatctacga ttcgtcatgg ccaccacaaa tgctacgctg caaacgctgg tacaatttta
     8881 cgaaaactgc aaaaacgtca aaactcggta taaaataatc aacgggcgct ttggcaaaat
     8941 atctatttta tcgcacaagc ccactagcaa attgtatttg cagaaaacaa tttcggcgca
     9001 caattttaac gctgacgaaa taaaagttca ccagttaatg agcgaccacc caaattttat
     9061 aaaaatctat tttaatcacg gttccatcaa caaccaagtg atcgtgatgg actacattga
     9121 ctgtcccgat ttatttgaaa cactacaaat taaaggcgag ctttcgtacc aacttgttag
     9181 caatattatt agacagctgt gtgaagcgct caacgatttg cacaagcaca atttcataca
     9241 caacgacata aaactcgaaa atgtcttata tttcgaagca cttgatcgcg tgtatgtttg
     9301 cgattacgga ttgtgcaaac acgaaaactc acttagcgtg cacgacggca cgttggagta
     9361 ttttagtccg gaaaaaattc gacaccacaa ctatgcacgt tcgtttgact ggtacgccgt
     9421 cggcgtgtta acatacaagt tgctaaccgg cggccaattc gtaatcatgg tcatagctgt
     9481 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     9541 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     9601 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     9661 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     9721 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     9781 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     9841 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     9901 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     9961 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
    10021 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
    10081 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
    10141 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
    10201 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
    10261 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
    10321 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
    10381 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
    10441 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
    10501 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
    10561 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
    10621 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
    10681 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
    10741 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
    10801 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
    10861 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
    10921 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
    10981 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
    11041 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
    11101 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
    11161 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
    11221 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
    11281 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
    11341 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
    11401 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
    11461 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
    11521 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
    11581 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta
    11641 ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt
    11701 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagc
//
