LOCUS       sequence-274317-        9857 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  W2m.3.2-1 plasmid. Contains (TCF-LEF)7-BE3-P2A-Luc and is ppart of 
            the CAMERA2m system for cellular recording in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9857)
  AUTHORS   Tang W, Liu DR
  TITLE     Rewritable multi-event analog recording in bacterial and mammalian 
            cells.
  JOURNAL   Science. 2018 Feb 15. pii: science.aap8992. doi: 
            10.1126/science.aap8992.
  PUBMED    29449507
REFERENCE   2  (bases 1 to 9857)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2018 Feb 15. pii: science.aap8992. doi: 10.1126/science.aap8992."
COMMENT     Sequence Label: pWT065c
FEATURES             Location/Qualifiers
     source          1..9857
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        168..199
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     CDS             261..947
                     /codon_start=1
                     /product="cytidine deaminase (C to U editing enzyme) from 
                     rat"
                     /label=APOBEC-1
                     /note="can use ssDNA as a substrate (Komor et al., 2016)"
                     /translation="MSSETGPVAVDPTLRRRIEPHEFEVFFDPRELRKETCLLYEINWG
                     GRHSIWRHTSQNTNKHVEVNFIEKFTTERYFCPNTRCSITWFLSWSPCGECSRAITEFL
                     SRYPHVTLFIYIARLYHHADPRNRQGLRDLISSGVTIQIMTEQESGYCWRNFVNYSPSN
                     EAHWPRYPHLWVRLYVLELYCIILGLPPCLNILRRKQPQLTFFTIALQSCHYQRLPPHI
                     LWATGLK"
     CDS             join(996..4394,4395..4397,4398..4994,4995,4996..4998,
                     4999..5000,5001..5003,5004..5096)
                     /codon_start=1
                     /product="Streptococcus pyogenes Cas9 endonuclease with the
                     D1135V/R1335Q/T1337R mutations"
                     /label=Cas9 VQR
                     /note="has an altered recognition specificity for the 
                     protospacer adjacent motif (PAM) (Kleinstiver et al., 
                     2015)"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             join(996..1019,1020..1022,1023..5096)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             5109..5357
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             5370..5390
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5391..5447
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5448..7100
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(5514..5532)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     CDS             7106..7123
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(7146..7163)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7152..7376
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7447..7463)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7447..7463)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7460..7482)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7471..7487
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7495..7501,7502..7519,7520..7525))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7540..7561
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7678..7695)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7849..8437)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7929..7948)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8608..9399,9400..9468))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9231..9250
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9469..9573)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9652..9671)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 atcgataggt accgagctct tacgcgagat caaagggggt aagatcaaag ggggtaagat
       61 caaaggggcg cgagatcaaa gggggtaaga tcaaaggggg taagatcaaa gggggtaaga
      121 tcaaaggggc gcgcccgcgt gctagcccgg gctcgagatc tagactctag agggtatata
      181 atggaagctc gaattccagc ttggcattcc ggtactgttg gtaaaaagct tggcattccg
      241 gtactgttgg taaagccacc atgagctcag agactggccc agtggctgtg gaccccacat
      301 tgagacggcg gatcgagccc catgagtttg aggtattctt cgatccgaga gagctccgca
      361 aggagacctg cctgctttac gaaattaatt gggggggccg gcactccatt tggcgacata
      421 catcacagaa cactaacaag cacgtcgaag tcaacttcat cgagaagttc acgacagaaa
      481 gatatttctg tccgaacaca aggtgcagca ttacctggtt tctcagctgg agcccatgcg
      541 gcgaatgtag tagggccatc actgaattcc tgtcaaggta tccccacgtc actctgttta
      601 tttacatcgc aaggctgtac caccacgctg acccccgcaa tcgacaaggc ctgcgggatt
      661 tgatctcttc aggtgtgact atccaaatta tgactgagca ggagtcagga tactgctgga
      721 gaaactttgt gaattatagc ccgagtaatg aagcccactg gcctaggtat ccccatctgt
      781 gggtacgact gtacgttctt gaactgtact gcatcatact gggcctgcct ccttgtctca
      841 acattctgag aaggaagcag ccacagctga cattctttac catcgctctt cagtcttgtc
      901 attaccagcg actgccccca cacattctct gggccaccgg gttgaaaagc ggcagcgaga
      961 ctcccgggac ctcagagtcc gccacacccg aaagtgataa aaagtattct attggtttag
     1021 ccatcggcac taattccgtt ggatgggctg tcataaccga tgaatacaaa gtaccttcaa
     1081 agaaatttaa ggtgttgggg aacacagacc gtcattcgat taaaaagaat cttatcggtg
     1141 ccctcctatt cgatagtggc gaaacggcag aggcgactcg cctgaaacga accgctcgga
     1201 gaaggtatac acgtcgcaag aaccgaatat gttacttaca agaaattttt agcaatgaga
     1261 tggccaaagt tgacgattct ttctttcacc gtttggaaga gtccttcctt gtcgaagagg
     1321 acaagaaaca tgaacggcac cccatctttg gaaacatagt agatgaggtg gcatatcatg
     1381 aaaagtaccc aacgatttat cacctcagaa aaaagctagt tgactcaact gataaagcgg
     1441 acctgaggtt aatctacttg gctcttgccc atatgataaa gttccgtggg cactttctca
     1501 ttgagggtga tctaaatccg gacaactcgg atgtcgacaa actgttcatc cagttagtac
     1561 aaacctataa tcagttgttt gaagagaacc ctataaatgc aagtggcgtg gatgcgaagg
     1621 ctattcttag cgcccgcctc tctaaatccc gacggctaga aaacctgatc gcacaattac
     1681 ccggagagaa gaaaaatggg ttgttcggta accttatagc gctctcacta ggcctgacac
     1741 caaattttaa gtcgaacttc gacttagctg aagatgccaa attgcagctt agtaaggaca
     1801 cgtacgatga cgatctcgac aatctactgg cacaaattgg agatcagtat gcggacttat
     1861 ttttggctgc caaaaacctt agcgatgcaa tcctcctatc tgacatactg agagttaata
     1921 ctgagattac caaggcgccg ttatccgctt caatgatcaa aaggtacgat gaacatcacc
     1981 aagacttgac acttctcaag gccctagtcc gtcagcaact gcctgagaaa tataaggaaa
     2041 tattctttga tcagtcgaaa aacgggtacg caggttatat tgacggcgga gcgagtcaag
     2101 aggaattcta caagtttatc aaacccatat tagagaagat ggatgggacg gaagagttgc
     2161 ttgtaaaact caatcgcgaa gatctactgc gaaagcagcg gactttcgac aacggtagca
     2221 ttccacatca aatccactta ggcgaattgc atgctatact tagaaggcag gaggattttt
     2281 atccgttcct caaagacaat cgtgaaaaga ttgagaaaat cctaaccttt cgcatacctt
     2341 actatgtggg acccctggcc cgagggaact ctcggttcgc atggatgaca agaaagtccg
     2401 aagaaacgat tactccatgg aattttgagg aagttgtcga taaaggtgcg tcagctcaat
     2461 cgttcatcga gaggatgacc aactttgaca agaatttacc gaacgaaaaa gtattgccta
     2521 agcacagttt actttacgag tatttcacag tgtacaatga actcacgaaa gttaagtatg
     2581 tcactgaggg catgcgtaaa cccgcctttc taagcggaga acagaagaaa gcaatagtag
     2641 atctgttatt caagaccaac cgcaaagtga cagttaagca attgaaagag gactacttta
     2701 agaaaattga atgcttcgat tctgtcgaga tctccggggt agaagatcga tttaatgcgt
     2761 cacttggtac gtatcatgac ctcctaaaga taattaaaga taaggacttc ctggataacg
     2821 aagagaatga agatatctta gaagatatag tgttgactct taccctcttt gaagatcggg
     2881 aaatgattga ggaaagacta aaaacatacg ctcacctgtt cgacgataag gttatgaaac
     2941 agttaaagag gcgtcgctat acgggctggg gacgattgtc gcggaaactt atcaacggga
     3001 taagagacaa gcaaagtggt aaaactattc tcgattttct aaagagcgac ggcttcgcca
     3061 ataggaactt tatgcagctg atccatgatg actctttaac cttcaaagag gatatacaaa
     3121 aggcacaggt ttccggacaa ggggactcat tgcacgaaca tattgcgaat cttgctggtt
     3181 cgccagccat caaaaagggc atactccaga cagtcaaagt agtggatgag ctagttaagg
     3241 tcatgggacg tcacaaaccg gaaaacattg taatcgagat ggcacgcgaa aatcaaacga
     3301 ctcagaaggg gcaaaaaaac agtcgagagc ggatgaagag aatagaagag ggtattaaag
     3361 aactgggcag ccagatctta aaggagcatc ctgtggaaaa tacccaattg cagaacgaga
     3421 aactttacct ctattaccta caaaatggaa gggacatgta tgttgatcag gaactggaca
     3481 taaaccgttt atctgattac gacgtcgatc acattgtacc ccaatccttt ttgaaggacg
     3541 attcaatcga caataaagtg cttacacgct cggataagaa ccgagggaaa agtgacaatg
     3601 ttccaagcga ggaagtcgta aagaaaatga agaactattg gcggcagctc ctaaatgcga
     3661 aactgataac gcaaagaaag ttcgataact taactaaagc tgagaggggt ggcttgtctg
     3721 aacttgacaa ggccggattt attaaacgtc agctcgtgga aacccgccaa atcacaaagc
     3781 atgttgcaca gatactagat tcccgaatga atacgaaata cgacgagaac gataagctga
     3841 ttcgggaagt caaagtaatc actttaaagt caaaattggt gtcggacttc agaaaggatt
     3901 ttcaattcta taaagttagg gagataaata actaccacca tgcgcacgac gcttatctta
     3961 atgccgtcgt agggaccgca ctcattaaga aatacccgaa gctagaaagt gagtttgtgt
     4021 atggtgatta caaagtttat gacgtccgta agatgatcgc gaaaagcgaa caggagatag
     4081 gcaaggctac agccaaatac ttcttttatt ctaacattat gaatttcttt aagacggaaa
     4141 tcactctggc aaacggagag atacgcaaac gacctttaat tgaaaccaat ggggagacag
     4201 gtgaaatcgt atgggataag ggccgggact tcgcgacggt gagaaaagtt ttgtccatgc
     4261 cccaagtcaa catagtaaag aaaactgagg tgcagaccgg agggttttca aaggaatcga
     4321 ttcttccaaa aaggaatagt gataagctca tcgctcgtaa aaaggactgg gacccgaaaa
     4381 agtacggtgg cttcgatagc cctacagttg cctattctgt cctagtagtg gcaaaagttg
     4441 agaagggaaa atccaagaaa ctgaagtcag tcaaagaatt attggggata acgattatgg
     4501 agcgctcgtc ttttgaaaag aaccccatcg acttccttga ggcgaaaggt tacaaggaag
     4561 taaaaaagga tctcataatt aaactaccaa agtatagtct gtttgagtta gaaaatggcc
     4621 gaaaacggat gttggctagc gccggagagc ttcaaaaggg gaacgaactc gcactaccgt
     4681 ctaaatacgt gaatttcctg tatttagcgt cccattacga gaagttgaaa ggttcacctg
     4741 aagataacga acagaagcaa ctttttgttg agcagcacaa acattatctc gacgaaatca
     4801 tagagcaaat ttcggaattc agtaagagag tcatcctagc tgatgccaat ctggacaaag
     4861 tattaagcgc atacaacaag cacagggata aacccatacg tgagcaggcg gaaaatatta
     4921 tccatttgtt tactcttacc aacctcggcg ctccagccgc attcaagtat tttgacacaa
     4981 cgatagatcg caaacgatac acttctacca aggaggtgct agacgcgaca ctgattcacc
     5041 aatccatcac gggattatat gaaactcgga tagatttgtc acagcttggg ggtgactctg
     5101 gtggttctac taatctgtca gatattattg aaaaggagac cggtaagcaa ctggttatcc
     5161 aggaatccat cctcatgctc ccagaggagg tggaagaagt cattgggaac aagccggaaa
     5221 gcgatatact cgtgcacacc gcctacgacg agagcaccga cgagaatgtc atgcttctga
     5281 ctagcgacgc ccctgaatac aagccttggg ctctggtcat acaggatagc aacggtgaga
     5341 acaagattaa gatgctctct ggtggttctc ccaagaagaa gaggaaagtc gctactaact
     5401 tcagcctgct gaagcaggct ggagacgtgg aggagaaccc tggacctatg gaagacgcca
     5461 aaaacataaa gaaaggcccg gcgccattct atccgctgga agatggaacc gctggagagc
     5521 aactgcataa ggctatgaag agatacgccc tggttcctgg aacaattgct tttacagatg
     5581 cacatatcga ggtggacatc acttacgctg agtacttcga aatgtccgtt cggttggcag
     5641 aagctatgaa acgatatggg ctgaatacaa atcacagaat cgtcgtatgc agtgaaaact
     5701 ctcttcaatt ctttatgccg gtgttgggcg cgttatttat cggagttgca gttgcgcccg
     5761 cgaacgacat ttataatgaa cgtgaattgc tcaacagtat gggcatttcg cagcctaccg
     5821 tggtgttcgt ttccaaaaag gggttgcaaa aaattttgaa cgtgcaaaaa aagctcccaa
     5881 tcatccaaaa aattattatc atggattcta aaacggatta ccagggattt cagtcgatgt
     5941 acacgttcgt cacatctcat ctacctcccg gttttaatga atacgatttt gtgccagagt
     6001 ccttcgatag ggacaagaca attgcactga tcatgaactc ctctggatct actggtctgc
     6061 ctaaaggtgt cgctctgcct catagaactg cctgcgtgag attctcgcat gccagagatc
     6121 ctatttttgg caatcaaatc attccggata ctgcgatttt aagtgttgtt ccattccatc
     6181 acggttttgg aatgtttact acactcggat atttgatatg tggatttcga gtcgtcttaa
     6241 tgtatagatt tgaagaagag ctgtttctga ggagccttca ggattacaag attcaaagtg
     6301 cgctgctggt gccaacccta ttctccttct tcgccaaaag cactctgatt gacaaatacg
     6361 atttatctaa tttacacgaa attgcttctg gtggcgctcc cctctctaag gaagtcgggg
     6421 aagcggttgc caagaggttc catctgccag gtatcaggca aggatatggg ctcactgaga
     6481 ctacatcagc tattctgatt acacccgagg gggatgataa accgggcgcg gtcggtaaag
     6541 ttgttccatt ttttgaagcg aaggttgtgg atctggatac cgggaaaacg ctgggcgtta
     6601 atcaaagagg cgaactgtgt gtgagaggtc ctatgattat gtccggttat gtaaacaatc
     6661 cggaagcgac caacgccttg attgacaagg atggatggct acattctgga gacatagctt
     6721 actgggacga agacgaacac ttcttcatcg ttgaccgcct gaagtctctg attaagtaca
     6781 aaggctatca ggtggctccc gctgaattgg aatccatctt gctccaacac cccaacatct
     6841 tcgacgcagg tgtcgcaggt cttcccgacg atgacgccgg tgaacttccc gccgccgttg
     6901 ttgttttgga gcacggaaag acgatgacgg aaaaagagat cgtggattac gtcgccagtc
     6961 aagtaacaac cgcgaaaaag ttgcgcggag gagttgtgtt tgtggacgaa gtaccgaaag
     7021 gtcttaccgg aaaactcgac gcaagaaaaa tcagagagat cctcataaag gccaagaagg
     7081 gcggaaagat cgccgtgtaa ccggtcatca tcaccatcac cattgagttt aaacccgctg
     7141 atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc
     7201 ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc
     7261 atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa
     7321 gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc
     7381 tgaggcggaa agaaccagct ggggctcgat accgtcgacc tctagctaga gcttggcgta
     7441 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     7501 acgagccgga agcataaagt gtaaagccta gggtgcctaa tgagtgagct aactcacatt
     7561 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     7621 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     7681 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     7741 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     7801 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     7861 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     7921 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     7981 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     8041 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     8101 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     8161 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     8221 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     8281 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     8341 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     8401 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     8461 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     8521 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     8581 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     8641 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     8701 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     8761 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     8821 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     8881 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     8941 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     9001 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     9061 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     9121 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     9181 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     9241 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     9301 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     9361 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     9421 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     9481 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     9541 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
     9601 cgtcgacgga tcgggagatc gatctcccga tcccctaggg tcgactctca gtacaatctg
     9661 ctctgatgcc gcatagttaa gccagtatct gctccctgct tgtgtgttgg aggtcgctga
     9721 gtagtgcgcg agcaaaattt aagctacaac aaggcaaggc ttgaccgaca attgcatgaa
     9781 gaatctgctt agggttaggc gttttgcgct gcttcgcgat gtacgggcca gatatacgcg
     9841 ttgacattga aggccct
//
