LOCUS       sequence-274319-        8439 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  to make YFP-Parkin stable cell line with retrovirus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8439)
  AUTHORS   Yamano K, Fogel AI, Wang C, van der Bliek AM, Youle RJ
  TITLE     Mitochondrial Rab GAPs govern autophagosome biogenesis during 
            mitophagy.
  JOURNAL   Elife (Cambridge). 2014 Feb 25;3:e01612. doi: 10.7554/eLife.01612.
  PUBMED    24569479
REFERENCE   2  (bases 1 to 8439)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife 
            (Cambridge)."; date: "2014-02-25"; volume: "3:e01612. doi"; pages: "
            10.7554/eLife.01612"
COMMENT     Sequence Label: pBMN-YFP-Parkin
FEATURES             Location/Qualifiers
     source          1..8439
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..470
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    535..892
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             957..1373
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1303..1325
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1341..1357
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    1383..1757
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     1695..1711
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     CDS             join(1766..1768,1769..1771,1772..2482)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1811..1832)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2072..2091)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2419..2440
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             4078..4547
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(4677..4694)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4848..5436)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4928..4947)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5600..6391,6392..6460))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6223..6242
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6461..6565)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctgaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa
       61 gaacagatgg aacagctgaa tatgggccaa acaggatatc tgtggtaagc agttcctgcc
      121 ccggctcagg gccaagaaca gatggtcccc agatgcggtc cagccctcag cagtttctag
      181 agaaccatca gatgtttcca gggtgcccca aggacctgaa atgaccctgt gccttatttg
      241 aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc ttctgctccc cgagctcaat
      301 aaaagagccc acaacccctc actcggcgcg ccagtcctcc gattgactga gtcgcccggg
      361 tacccgtgta tccaataaac cctcttgcag ttgcatccga cttgtggtct cgctgttcct
      421 tgggagggtc tcctctgagt gattgactac ccgtcagcgg gggtctttca tttgggggct
      481 cgtccgggat cgggagaccc ctgcccaggg accaccgacc caccaccggg aggtaagctg
      541 gccagcaact tatctgtgtc tgtccgattg tctagtgtct atgactgatt ttatgcgcct
      601 gcgtcggtac tagttagcta actagctctg tatctggcgg acccgtggtg gaactgacga
      661 gttcggaaca cccggccgca accctgggag acgtcccagg gacttcgggg gccgtttttg
      721 tggcccgacc tgagtccaaa aatcccgatc gttttggact ctttggtgca ccccccttag
      781 aggagggata tgtggttctg gtaggagacg agaacctaaa acagttcccg cctccgtctg
      841 aatttttgct ttcggtttgg gaccgaagcc gcgccgcgcg tcttgtctgc tgcagcatcg
      901 ttctgtgttg tctctgtctg actgtgtttc tgtatttgtc tgaaaatatg ggcccgggcc
      961 agactgttac cactccctta agtttgacct taggtcactg gaaagatgtc gagcggatcg
     1021 ctcacaacca gtcggtagat gtcaagaaga gacgttgggt taccttctgc tctgcagaat
     1081 ggccaacctt taacgtcgga tggccgcgag acggcacctt taaccgagac ctcatcaccc
     1141 aggttaagat caaggtcttt tcacctggcc cgcatggaca cccagaccag gtcccctaca
     1201 tcgtgacctg ggaagccttg gcttttgacc cccctccctg ggtcaagccc tttgtacacc
     1261 ctaagcctcc gcctcctctt cctccatccg ccccgtctct cccccttgaa cctcctcgtt
     1321 cgaccccgcc tcgatcctcc ctttatccag ccctcactcc ttctctaggc gcccccatat
     1381 ggccatatga gatcttatat ggggcacccc cgccccttgt aaacttccct gaccctgaca
     1441 tgacaagagt tactaacagc ccctctctcc aagctcactt acaggctctc tacttagtcc
     1501 agcacgaagt ctggagacct ctggcggcag cctaccaaga acaactggac cgaccggtgg
     1561 tacctcaccc ttaccgagtc ggcgacacag tgtgggtccg ccgacaccag actaagaacc
     1621 tagaacctcg ctggaaagga ccttacacag tcctgctgac cacccccacc gccctcaaag
     1681 tagacggcat cgcagcttgg atacacgccg cccacgtgaa ggctgccgac cccgggggtg
     1741 gaccatcctc tagactgccg gatccatggt gagcaagggc gaggagctgt tcaccggggt
     1801 ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg
     1861 cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg
     1921 caagctgccc gtgccctggc ccaccctcgt gaccaccttc ggctacggcc tgcagtgctt
     1981 cgcccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg
     2041 ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga
     2101 ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa
     2161 ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta
     2221 tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat
     2281 cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg
     2341 ccccgtgctg ctgcccgaca accactacct gagctaccag tccgccctga gcaaagaccc
     2401 caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct
     2461 cggcatggac gagctgtaca agtccggact cagatctcga gctcaagctt tgatagtgtt
     2521 tgtcaggttc aactccagcc atggtttccc agtggaggtc gattctgaca ccagcatctt
     2581 ccagctcaag gaggtggttg ctaagcgaca gggggttccg gctgaccagt tgcgtgtgat
     2641 tttcgcaggg aaggagctga ggaatgactg gactgtgcag aattgtgacc tggatcagca
     2701 gagcattgtt cacattgtgc agagaccgtg gagaaaaggt caagaaatga atgcaactgg
     2761 aggcgacgac cccagaaacg cggcgggagg ctgtgagcgg gagccccaga gcttgactcg
     2821 ggtggacctc agcagctcag tcctcccagg agactctgtg gggctggctg tcattctgca
     2881 cactgacagc aggaaggact caccaccagc tggaagtcca gcaggtagat caatctacaa
     2941 cagcttttat gtgtattgca aaggcccctg tcaaagagtg cagccgggaa aactcagggt
     3001 acagtgcagc acctgcaggc aggcaacgct caccttgacc cagggtccat cttgctggga
     3061 tgatgtttta attccaaacc ggatgagtgg tgaatgccaa tccccacact gccctgggac
     3121 tagtgcagaa tttttcttta aatgtggagc acaccccacc tctgacaagg aaacatcagt
     3181 agctttgcac ctgatcgcaa caaatagtcg gaacatcact tgcattacgt gcacagacgt
     3241 caggagcccc gtcctggttt tccagtgcaa ctcccgccac gtgatttgct tagactgttt
     3301 ccacttatac tgtgtgacaa gactcaatga tcggcagttt gtccacgacc ctcagcttgg
     3361 ctactccctg ccttgtgtgg ctggctgtcc caactccttg attaaagagc tccatcactt
     3421 caggattctg ggagaagagc agtacaaccg gtaccagcag tatggtgcag aggagtgtgt
     3481 cctgcagatg gggggcgtgt tatgcccccg ccctggctgt ggagcggggc tgctgccgga
     3541 gcctgaccag aggaaagtca cctgcgaagg gggcaatggc ctgggctgtg ggtttgcctt
     3601 ctgccgggaa tgtaaagaag cgtaccatga aggggagtgc agtgccgtat ttgaagcctc
     3661 aggaacaact actcaggcct acagagtcga tgaaagagcc gccgagcagg ctcgttggga
     3721 agcagcctcc aaagaaacca tcaagaaaac caccaagccc tgtccccgct gccatgtacc
     3781 agtggaaaaa aatggaggct gcatgcacat gaagtgtccg cagccccagt gcaggctcga
     3841 gtggtgctgg aactgtggct gcgagtggaa ccgcgtctgc atgggggacc actggttcga
     3901 cgtgtaagtc gacgataaaa taaaagattt tatttagtct ccagaaaaag gggggaatga
     3961 aagaccccac ctgtaggttt ggcaagctag cttaagtaac gccattttgc aaggcatgga
     4021 aaaatacata actgagaata gagaagttca gatcaaggtc aggaacagat ggaacagctg
     4081 aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca gggccaagaa
     4141 cagatggaac agctgaatat gggccaaaca ggatatctgt ggtaagcagt tcctgccccg
     4201 gctcagggcc aagaacagat ggtccccaga tgcggtccag ccctcagcag tttctagaga
     4261 accatcagat gtttccaggg tgccccaagg acctgaaatg accctgtgcc ttatttgaac
     4321 taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga gctcaataaa
     4381 agagcccaca acccctcact cggggcgcca gtcctccgat tgactgagtc gcccgggtac
     4441 ccgtgtatcc aataaaccct cttgcagttg catccgactt gtggtctcgc tgttccttgg
     4501 gagggtctcc tctgagtgat tgactacccg tcagcggggg tctttcacat gcagcatgta
     4561 tcaaaattaa tttggttttt tttcttaagt atttacatta aatggccata gttgcattaa
     4621 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     4681 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     4741 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     4801 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     4861 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     4921 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     4981 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     5041 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     5101 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     5161 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     5221 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     5281 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     5341 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     5401 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     5461 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     5521 aaaaggatct tcacctagat ccttttgcgg ccggccgcaa atcaatctaa agtatatatg
     5581 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     5641 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     5701 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     5761 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     5821 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     5881 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     5941 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     6001 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     6061 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     6121 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     6181 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     6241 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     6301 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     6361 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     6421 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     6481 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     6541 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacca gctttgctct
     6601 taggagtttc ctaatacatc ccaaactcaa atatataaag catttgactt gttctatgcc
     6661 ctagggggcg gggggaagct aagccctgag agatcccctc ataatttccc caaaacgtaa
     6721 ccatgtgtga atagattttg agtaagcagg gttgcagcca cgagtgagtc ttcccttgtt
     6781 attgtgtagc cagaatgccg caaaacttcc atgcctaagc gaactgttga gagtacgttt
     6841 cgatttctga ctgtgttagc ctggaagtgc ttgtcccaac cttgtttctg agcatgaacg
     6901 cccgcaagcc aacatgttag ttgaagcatc agggcgatta gcagcatgat atcaaaacgc
     6961 tctgagctgc tcgttcggct atggcgtagg cctagtccgt aggcaggact tttcaagtct
     7021 cggaaggttt cttcaatctg cattcgcttc gaatagatat taacaagttg tttgggtgtt
     7081 cgaatttcaa caggtaagtt agttgctaga atccatggct cctttgccga cgctgagtag
     7141 attttaggtg acgggtggtg acaatgagtc cgtgtcgagc gctgattttt tcggccttta
     7201 gagcgagatt tatacaatag aatttggcat gagattgggt tgcttttagt cagcctctta
     7261 tagcctaaag tctttgagtg actagatgac atatcatgta agttgctgat aggtttccag
     7321 ttttccgctc ctaggtctgc atattgtact tttcctctta ctcgacttaa ccagtaccaa
     7381 cccagcttct caacggattt ataccatggc actttaaagc cagcatcact gacaatgagc
     7441 ggtgtggtgt tactcggtag aatgctcgca aggtcggcta gaaattggtc atgagctttc
     7501 tttgaacatt gctctgaaag cgggaacgct ttctcataaa gagtaacaga acgaccgtgt
     7561 agtgcgactg aagctcgcaa taccataagc cgtttttgct cacggatatc agaccagtca
     7621 acaagtacaa tgggcatcgt attgcccgaa cagataaagc tagcatgcca acggtataca
     7681 gcgagtcgct ctttgtggag gtgacgatta cctaacaatc ggtcgattcg tttgatgtta
     7741 tgttttgttc tcgctttggt tggcaggtta cggccaagtt cggtaagagt gagagtttta
     7801 cagtcaagta aggcgtggca agccaacgtt aagctgttga gtcgttttaa gtgtaattcg
     7861 gggcagaatt ggtaaagaga gtcgtgtaaa atatcgagtt cgcacatttt gttgtctgat
     7921 tattgatttt tggcgaaacc atttgatcat atgacaagat gtgtatctac cttaacttaa
     7981 tgattttgat aaaaatcatt aggggattca tcagagctaa gccagctttt tttaacattt
     8041 aaaatgttaa ttccatttta aatgcacaga tgtttttatt tcataagggt ttcaatgtgc
     8101 atgaatgctg caatattcct gttaccaaag ctagtataaa taaaaataga taaacgtgga
     8161 aattacttag agtttctgtc attaacgttt ccttcctcag ttgacaacat aaatgcgctg
     8221 ctgagaagcc agtttgcatc tgtcaggatc aatttcccat tatgccagtc atattaatta
     8281 ctagtcaatt agttgatttt tatttttgac atatacatgt gaaagacccc acctgtaggt
     8341 ttggcaagct agcttaagta acgccatttt gcaaggcatg gaaaaataca taactgagaa
     8401 tagaaaagtt cagatcaagg tcaggaacag atggaacag
//
