LOCUS       sequence-274326-        5778 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Part Plasmid for dCas9, Part 3(coding sequence).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5778)
  AUTHORS   Geller SH, Antwi EB, Di Ventura B, McClean MN
  TITLE     Optogenetic Repressors of Gene Expression in Yeasts Using 
            Light-Controlled Nuclear Localization.
  JOURNAL   Cell Mol Bioeng. 2019 Sep 24;12(5):511-528. doi: 
            10.1007/s12195-019-00598-9. eCollection 2019 Oct.
  PUBMED    31719930
REFERENCE   2  (bases 1 to 5778)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1007/s12195-019-00598-9"; journalName: "Cell Mol Bioeng"; date: 
            "2019-09-24- 24"; volume: "12"; issue: "5"; pages: "511-528"
COMMENT     Sequence Label: pMM586
FEATURES             Location/Qualifiers
     source          1..5778
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(4134..4168)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(4239..4898)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     4813..4832
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      5080..5668
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5569..5588
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tatggacaag aagtattcta tcggactggc catcgggact aatagcgtcg ggtgggccgt
       61 gatcactgac gagtacaagg tgccctctaa gaagttcaag gtgctcggga acaccgaccg
      121 gcattccatc aagaaaaatc tgatcggagc tctcctcttt gattcagggg aaaccgctga
      181 agcaacccgc ctcaagcgga ctgctagacg gcggtacacc aggaggaaga accggatttg
      241 ttaccttcaa gagatattct ccaacgaaat ggcaaaggtc gacgacagct tcttccatag
      301 gctggaagaa tcattcctcg tggaagagga taagaagcat gaacggcatc ccatcttcgg
      361 taatatcgtc gacgaggtgg cctatcacga gaaataccca accatctacc atcttcgcaa
      421 aaagctggtg gactcaaccg acaaggcaga cctccggctt atctacctgg ccctggccca
      481 catgatcaag ttcagaggcc acttcctgat cgagggcgac ctcaatcctg acaatagcga
      541 tgtggataaa ctgttcatcc agctggtgca gacttacaac cagctctttg aagagaaccc
      601 catcaatgca agcggagtcg atgccaaggc cattctgtca gcccggctgt caaagagccg
      661 cagacttgag aatcttatcg ctcagctgcc gggtgaaaag aaaaatggac tgttcgggaa
      721 cctgattgct ctttcacttg ggctgactcc caatttcaag tctaatttcg acctggcaga
      781 ggatgccaag ctgcaactgt ccaaggacac ctatgatgac gatctcgaca acctcctggc
      841 ccagatcggt gaccaatacg ccgacctttt ccttgctgct aagaatcttt ctgacgccat
      901 cctgctgtct gacattctcc gcgtgaacac tgaaatcacc aaggcccctc tttcagcttc
      961 aatgattaag cggtatgatg agcaccacca ggacctgacc ctgcttaagg cactcgtccg
     1021 gcagcagctt ccggagaagt acaaggaaat cttctttgac cagtcaaaga atggatacgc
     1081 cggctacatc gacggaggtg cctcccaaga ggaattttat aagtttatca aacctatcct
     1141 tgagaagatg gacggcaccg aagagctcct cgtgaaactg aatcgggagg atctgctgcg
     1201 gaagcagcgc actttcgaca atgggagcat tccccaccag atccatcttg gggagcttca
     1261 cgccatcctt cggcgccaag aggacttcta cccctttctt aaggacaaca gggagaagat
     1321 tgagaaaatt ctcactttcc gcatccccta ctacgtggga cccctcgcca gaggaaatag
     1381 ccggtttgct tggatgacca gaaagtcaga agaaactatc actccctgga acttcgaaga
     1441 ggtggtggac aagggagcca gcgctcagtc attcatcgaa cggatgacta acttcgataa
     1501 gaacctcccc aatgagaagg tcctgccgaa acattccctg ctctacgagt actttaccgt
     1561 gtacaacgag ctgaccaagg tgaaatatgt caccgaaggg atgaggaagc ccgcattcct
     1621 gtcaggcgaa caaaagaagg caattgtgga ccttctgttc aagaccaata gaaaggtgac
     1681 cgtgaagcag ctgaaggagg actatttcaa gaaaattgaa tgcttcgact ctgtggagat
     1741 tagcggggtc gaagatcggt tcaacgcaag cctgggtacc taccatgatc tgcttaagat
     1801 catcaaggac aaggattttc tggacaatga ggagaaagag gacatccttg aggacattgt
     1861 cctgactctc actctgttcg aggaccggga aatgatcgag gagaggctta agacctacgc
     1921 ccatctgttc gacgataaag tgatgaagca acttaaacgg agaagatata ccggatgggg
     1981 acgccttagc cgcaaactca tcaacggaat ccgggacaaa cagagcggaa agaccattct
     2041 tgatttcctt aagagcgacg gattcgctaa tcgcaacttc atgcaactta tccatgatga
     2101 ttccctgacc tttaaggagg acatccagaa ggcccaagtg tctggacaag gtgactcact
     2161 gcacgagcat atcgcaaatc tggctggttc acccgctatt aagaagggta ttctccagac
     2221 cgtgaaagtc gtggacgagc tggtcaaggt gatgggtcgc cataaaccag agaacattgt
     2281 catcgagatg gccagggaaa accagactac ccagaaggga cagaagaaca gcagggagcg
     2341 gatgaaaaga attgaggaag ggattaagga gctcgggtca cagatcctta aagagcaccc
     2401 ggtggaaaac acccagcttc agaatgagaa gctctatctg tactaccttc aaaatggacg
     2461 cgatatgtat gtggaccaag agcttgatat caacaggctc tcagactacg acgtggacgc
     2521 catcgtccct cagagcttcc tcaaagacga ctcaattgac aataaggtgc tgactcgctc
     2581 agacaagaac cggggaaagt cagataacgt gccctcagag gaagtcgtga aaaagatgaa
     2641 gaactattgg cgccagcttc tgaacgcaaa gctgatcact cagcggaagt tcgacaatct
     2701 cactaaggct gagaggggcg gactgagcga actggacaaa gcaggattca ttaaacggca
     2761 acttgtggag actcggcaga ttactaaaca tgtagcccaa atccttgact cacgcatgaa
     2821 taccaagtac gacgaaaacg acaaacttat ccgcgaggtg aaggtgatta ccctgaagtc
     2881 caagctggtc agcgatttca gaaaggactt tcaattctac aaagtgcggg agatcaataa
     2941 ctatcatcat gctcatgacg catatctgaa tgccgtggtg ggaaccgccc tgatcaagaa
     3001 gtacccaaag ctggaaagcg agttcgtgta cggagactac aaggtctacg acgtgcgcaa
     3061 gatgattgcc aaatctgagc aggagatcgg aaaggccacc gcaaagtact tcttctacag
     3121 caacatcatg aatttcttca agaccgaaat cacccttgca aacggtgaga tccggaagag
     3181 gccgctcatc gagactaatg gggagactgg cgaaatcgtg tgggacaagg gcagagattt
     3241 cgctaccgtg cgcaaagtgc tttctatgcc tcaagtgaac atcgtgaaga aaaccgaggt
     3301 gcaaaccgga ggcttttcta aggaatcaat cctccccaag cgcaactccg acaagctcat
     3361 tgcaaggaag aaggattggg accctaagaa gtacggcgga ttcgattcac caactgtggc
     3421 ttattctgtc ctggtcgtgg ctaaggtgga aaaaggaaag tctaagaagc tcaagagcgt
     3481 gaaggaactg ctgggtatca ccattatgga gcgcagctcc ttcgagaaga acccaattga
     3541 ctttctcgaa gccaaaggtt acaaggaagt caagaaggac cttatcatca agctcccaaa
     3601 gtatagcctg ttcgaactgg agaatgggcg gaagcggatg ctcgcctccg ctggcgaact
     3661 tcagaagggt aatgagctgg ctctcccctc caagtacgtg aatttcctct accttgcaag
     3721 ccattacgag aagctgaagg ggagccccga ggacaacgag caaaagcaac tgtttgtgga
     3781 gcagcataag cattatctgg acgagatcat tgagcagatt tccgagtttt ctaaacgcgt
     3841 cattctcgct gatgccaacc tcgataaagt ccttagcgca tacaataagc acagagacaa
     3901 accaattcgg gagcaggctg agaatatcat ccacctgttc accctcacca atcttggtgc
     3961 ccctgccgca ttcaagtact tcgacaccac catcgaccgg aaacgctata cctccaccaa
     4021 agaagtgctg gacgccaccc tcatccacca gagcatcacc ggactttacg aaactcggat
     4081 tgacctctca cagctcggag gggatgaggg agctggatcc tgagaccaga ccaataaaaa
     4141 acgcccggcg gcaaccgagc gttctgaaca aatccagatg gagttctgag gtcattactg
     4201 gatctatcaa caggagtcca agcgagctcg atatcaaatt acgccccgcc ctgccactca
     4261 tcgcagtact gttgtaattc attaagcatt ctgccgacat ggaagccatc acaaacggca
     4321 tgatgaacct gaatcgccag cggcatcagc accttgtcgc cttgcgtata atatttgccc
     4381 atggtgaaaa cgggggcgaa gaagttgtcc atattggcca cgtttaaatc aaaactggtg
     4441 aaactcaccc agggattggc tgaaacgaaa aacatattct caataaaccc tttagggaaa
     4501 taggccaggt tttcaccgta acacgccaca tcttgcgaat atatgtgtag aaactgccgg
     4561 aaatcgtcgt ggtattcact ccagagcgat gaaaacgttt cagtttgctc atggaaaacg
     4621 gtgtaacaag ggtgaacact atcccatatc accagctcac cgtctttcat tgccatacga
     4681 aattccggat gagcattcat caggcgggca agaatgtgaa taaaggccgg ataaaacttg
     4741 tgcttatttt tctttacggt ctttaaaaag gccgtaatat ccagctgaac ggtctggtta
     4801 taggtacatt gagcaactga ctgaaatgcc tcaaaatgtt ctttacgatg ccattgggat
     4861 atatcaacgg tggtatatcc agtgattttt ttctccattt tagcttcctt agctcctgaa
     4921 aatctcgata actcaaaaaa tacgcccggt agtgatctta tttcattatg gtgaaagttg
     4981 gaacctctta cgtgcccgat caatcatgac caaaatccct taacgtgagt tttcgttcca
     5041 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     5101 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     5161 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     5221 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     5281 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     5341 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     5401 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     5461 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     5521 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     5581 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     5641 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     5701 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     5761 taaccgtagt cggtctca
//
