LOCUS       sequence-27433-e         728 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 728)
  AUTHORS   Ren X, Kloer DP, Kim YC, Ghirlando R, Saidi LF, Hummer G, Hurley JH
  TITLE     Hybrid structural model of the complete human ESCRT-0 complex.
  JOURNAL   Structure. 2009 Mar 11. 17(3):406-16.
  PUBMED    19278655
REFERENCE   2  (bases 1 to 728)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Structure. 
            2009 Mar 11. 17(3):406-16."
COMMENT     Sequence Label: pST39-Hrs-STAM1 core
FEATURES             Location/Qualifiers
     source          1..728
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             5..27
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     392..409
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     393..409
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     RBS             416..438
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             458..475
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             497..517
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 taattttgtt taactttaag aaggagatat acatatgaat ggcgagtctg aggagagcca
       61 cgagcagttc ctgaaggcgc tgcagaacgc cgtcaccacc ttcgtgaacc gcatgaagag
      121 taaccacatg cggggccgca gcatcaccaa tgactcggcc gtgctctcac tcttccagtc
      181 catcaacggc atgcacccgc agctgctgga gctgctcaac cagctggacg agcgcaggct
      241 gtactatgag gggctgcagg acaagctggc acagatccgc gatgcccggg gggcgctgag
      301 tgccctgcgc gaagagcacc gggagaagct tcgccgggca gccgaggagg cagagcgcca
      361 gcgccagatc cagctggccc agaagctgta atgtaaaacg acggccagta ataattttgt
      421 ttaactttaa gaaggagata tacatatgtc gtactaccat caccatcacc atcacgatta
      481 cgatatccca acgaccgaaa acctgtattt tcagggcgcc atgtttattg atgaagataa
      541 aatggaccag ttgctacaga tgctgcaaag tacagacccc agtgatgatc agccagacct
      601 accagagctg cttcatcttg aagcaatgtg tcaccagatg ggacctctca ttgatgaaaa
      661 gctggaagat attgatagaa aacattcaga actctcagaa cttaatgtga aagtgatgga
      721 ggcccttt
//
