LOCUS       sequence-27434-e         756 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 756)
  AUTHORS   Ren X, Kloer DP, Kim YC, Ghirlando R, Saidi LF, Hummer G, Hurley JH
  TITLE     Hybrid structural model of the complete human ESCRT-0 complex.
  JOURNAL   Structure. 2009 Mar 11. 17(3):406-16.
  PUBMED    19278655
REFERENCE   2  (bases 1 to 756)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Structure. 
            2009 Mar 11. 17(3):406-16."
COMMENT     Sequence Label: pHIS2-hsSTAM1 (1-143)
FEATURES             Location/Qualifiers
     source          1..756
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             17..39
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             59..76
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             98..118
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             632..649
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(702..720)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 ttccctctag aataattttg tttaacttta agaaggagat atacatatgt cgtactacca
       61 tcaccatcac catcacgatt acgatatccc aacgaccgaa aacctgtatt ttcagggcgc
      121 catgggaatg cctctttttg ccaccaatcc cttcgatcag gatgttgaga aagcaaccag
      181 cgagatgaat actgctgagg actggggcct cattttggat atctgtgata aagttggtca
      241 gtctcgcact ggacctaagg attgtcttcg gtctattatg agaagagtga accacaaaga
      301 tcctcacgtt gctatgcagg ctttgactct tctaggagca tgtgtatcaa actgtggcaa
      361 aatttttcat ttagaagtat gttcaagaga ttttgctagt gaagtaagca acgtattaaa
      421 taagggtcat cctaaagtat gtgaaaaatt aaaggctctt atggttgaat ggacagatga
      481 atttaagaat gatccacagc ttagtctaat atcagcaatg attaagaacc tcaaggaaca
      541 aggagttacg ttcccataag gaattcaaag gcctacgtcg acgagctcac tagtcgcggc
      601 cgctttcgaa tctagagcct gcagtctcga gcaccaccac caccaccact gagatccggc
      661 tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc
      721 ataacccctt ggggcctcta aacgggtctt gagggg
//
