LOCUS       sequence-274347-       15232 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of a fungal biosynthetic gene cluster.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15232)
  AUTHORS   Harvey CJB, Tang M, Schlecht U, Horecka J, Fischer CR, Lin HC, Li J,
            Naughton B, Cherry J, Miranda M, Li YF, Chu AM, Hennessy JR, Vandova
            GA, Inglis D, Aiyar RS, Steinmetz LM, Davis RW, Medema MH, Sattely 
            E, Khosla C, St Onge RP, Tang Y, Hillenmeyer ME
  TITLE     HEx: A heterologous expression platform for the discovery of fungal 
            natural products.
  JOURNAL   Sci Adv. 2018 Apr 11;4(4):eaar5459. doi: 10.1126/sciadv.aar5459. 
            eCollection 2018 Apr.
  PUBMED    29651464
REFERENCE   2  (bases 1 to 15232)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2018-04-11"; volume: "4(4):eaar5459. doi"; pages: " 
            10.1126/sciadv.aar5459. eCollection 2018 Apr"
COMMENT     Sequence Label: pCHKU32.1-2.2
FEATURES             Location/Qualifiers
     source          1..15232
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1..17)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1..17)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             528..551
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(9680..9700)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(9680..9698)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     rep_origin      complement(10037..10625)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10117..10136)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10796..11587,11588..11656))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11419..11438
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11657..11761)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      11788..13130
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(12751..12798)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     13171..13189
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        13484..13891
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     CDS             13892..14986
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
ORIGIN
        1 gatccactag ttctagatta attaatatct aaaaattgcc ttatgatccg tctctccggt
       61 tacagcctgt gtaactgatt aatcctgcct ttctaatcac cattctaatg ttttaattaa
      121 gggattttgt cttcattaac ggctttcgct cataaaaatg ttatgacgtt ttgcccgcag
      181 gcgggaaacc atccacttca cgagactgat ctcctctgcc ggaacaccgg gcatctccaa
      241 cttataagtt ggagaaataa gagaatttca gattgagaga atgaaaaaaa aaaaaaaaaa
      301 aaggcagagg agagcataga aatggggttc actttttggt aaagctatag catgcctatc
      361 acatataaat agagtgccag tagcgacttt tttcacactc gaaatactct tactactgct
      421 ctcttgttgt ttttatcact tcttgtttct tcttggtaaa tagaatatca agctacaaaa
      481 agcatacaat caactatcaa ctattaacta tatcgtaata cacaatggat tataaggatg
      541 atgatgataa gactagtatg ggtagtaatg ccaaccccaa ctggctcgcg aaggagccta
      601 tcgcgatagt gggaagctcg tgtcgttttc caggcggcgc tagttccccc tcaaagcttt
      661 gggtgcttct caaggatcct cgggatatcg tgcaagaaat cccacccagt cgatttgata
      721 ccaaggcatt ttatcaccaa gacagccaac accatgggag tacaaacgca aagtatgcct
      781 acctcctgca ggatgacccc cgggctttcg accgcgattt ctttaacatc agcccaaagg
      841 aggccgaggc aatggacccc caacagcgat gcctcctcga gacagtttac gaaggtattg
      901 aatcggccgg ctactccatc ccccagcttc ggggatcatc cactggcgtc tttgtgggag
      961 ccatgagctt cgactaccag ctcgttgcca tgcgtggcct cgacagcctg ccgcagtacc
     1021 atgcaaccgg cggttcgatg gcaatcctgg ccaaccgtgt gtcgtacttc tatgactgga
     1081 aaggcgcatc ggttgccttt gatacagcct gctcgtcatc gctcgttgcc ctacaccagg
     1141 cggtccttgc tctgcgaagc ggagaagtag acatggcggt tgcagcaggg gcgaatctca
     1201 tccttggacc tgagccattt attgcttatt caaatctaaa tatgctgtcc cccaacgggc
     1261 gatcatacat gtgggattct tctgccgatg gatatacgcg cggcgaggga ttcgctgcca
     1321 ttattctcaa gactcttagc caagctgtgg ctgatggtga ccacatcgag tgtattatcc
     1381 gtgaaacggg tgtgaattcc gatggccgga caccaggcat cacaatgccg aactctgagg
     1441 cacaagctcg acttatccga gacacataca ccaagtgcgg gctagacccg acgaacccaa
     1501 gggaccgtcc acaatacttc gaagcacatg gcaccggaac gccggtgggt gaccctatcg
     1561 aggcgcgggc aatacacgac gctttcttcc cggggcatcc agaccagaat catggccagc
     1621 tctatgttgg atcgatcaaa accgtcattg gtcacacaga gggcagtgca ggactggctg
     1681 gcgtgctcaa ggcgtcgttg gcggtccagc acggccagat tccaggtaac ctacatcttc
     1741 gggagctgaa ccccaaaatt cgaccattct tcaaccacct ccgcatcccg acagagttaa
     1801 ccccctggcc cgcgcttcca gagggccaac cgcgtcgtgc aagtgtgaat agtttcggct
     1861 ttggcgggac caatgcgcac gctatcatcg aaagctggga tgggggctac gagcgctatc
     1921 atgtgtcgga atcgcgtccg gctggactct tcgtcctatc tgctaactcg gcccaggccc
     1981 tggcagcaaa ggctgcagag ttagccaact atctacgaga tcatcccgac acagatcttg
     2041 gtaggctttc tcgcaccctg ttccagaggt cggaattccc gttccgggcc tccttctcag
     2101 ccacgtcggc cacccagctt gccgataagc tcgaaacagg cacagaagca ctcagcaaga
     2161 cgtcacgcat cgcaaccatt cccaattcac taccgccccg gatactcggt gtcttcactg
     2221 ggcaaggtgc tcagtgggct actatgggtt gggagctcta ccaagcatcc gccgtgttcc
     2281 gcacgtcgtt ggaccggcag cagcgaagcc tcgatacgct ccccgacggc gatcgcccgg
     2341 actggtccct gatcgacgag ctctcggcac caaaggaaac ctcccgcgtc ggtatcgcag
     2401 ccatctccca gcctctctgc actgctctcc aagtggcgct cgtagacgtc ctccgcacgg
     2461 ctggcgttga gttttctgcc gttgttggac actcgtcggg tgagattggt gccgcctatg
     2521 ctgcgggata cctggatgcc cgggatgcta ttcgtgtggc ttattatcga gggtttcatt
     2581 ctcatctggc acaggggccc ggaggaaagc gtggcaagat gatggctgtt ggcatgagct
     2641 tggatgaggc attggcattt tgcaacgagt ttagttcggc actcaaggtg gctgctaaca
     2701 actctccaac gagttgcacg ctggcgggag acgccgatgc tatcgatgag gccaaggcac
     2761 tgctggacca gagcaacact tttgcccgcg tgctggcggt cgacacagca tatcattctc
     2821 accacatgcg gccatgcgcc tcaccctatc tcgaatcgct acgcagttgt gggatcagcg
     2881 ttctgaaggg tcgcaaaaaa tgcgtgtggt actcgagtgt ttggggtgca aatggccgca
     2941 gtcgctcctt cgacgaccat catggtcttg agttgctcgg gggacagtac tgggtcgata
     3001 atctgaccag tacggtgcag ttctcccagg cagtaagcag agcagtgtct gaagaatcct
     3061 acgtcttgga tctcgccctt gaaattggac cgcacccggc tctcaggggt ccagcctccg
     3121 aggtgatcaa gtcgttgacg ggtgtagctg ttccgtattc tggtgtcctt aaacgcggag
     3181 agggtgctgt tgaggccttt gccgctggtt taggactcat ctggtcgttg ttcccgtcga
     3241 tgcgccctat catcaccttt gacggcttac accgagcctt cccccaagcc agcccaaagt
     3301 atcctgtaat tctcaaggac ctgccgcctt acccctggga tcatgatagc ctcatctggc
     3361 gtgagtccag agcatcttgc atcttccgca ctcagagcca gcctcgcgaa ctcctcggct
     3421 ttccggtcac actcggtgag catggccggc gcgaggttca ttggcgacag gtcttcaaac
     3481 taagtgaact gcaatgggtg aagggccatg ccatccaggg cgaagttctt ttcccggcaa
     3541 cggggtacct gaccatggcg tatgaggctg ctgtgcgtct cgtcaatgac catcaggcac
     3601 tgcgtcttgt cgagctgcat gacattgaga tggtgcgtgc catgagcttg caggaagatt
     3661 ccccgggtct tgaggttctc tttaccatac gtgttacgag ccagtccgac agctgcattg
     3721 ccgccgaggt ggcctgctac agcggcgata tcaacgtgac gaaactggac ggccctttga
     3781 cagggcttac agcccatttc agcggcagcg tgcggatttg gcttggcctg cccctgaaaa
     3841 acgccctgcc tccccggacg gagcctctgc tgcccatgaa cacgttggac ctggagcagt
     3901 tctactggta tttgtccaaa gtgggctaca actactcaga tgctttcaga gccagctcag
     3961 tggaccgccg ccttaaccag gccgtggtaa ccgtcccggc tccccctgag ccgactacaa
     4021 ttcggtcttc tatgcacccc acggtcctcg acactgccat ccaaggcctc ttggccgggt
     4081 tttcatatcc agaagacggt cgtctcagga cggtgtatct gccaaccgcc attgactgca
     4141 tccgtatcaa catggtggca ccccctgcgt ctcccggcgt cctcatggca gatagctttc
     4201 tgaccttggc ggatgcaaag tcacttgaag gcgacgtaga tgttttcgac tcaaaagatg
     4261 ccaccatcca tgtccaactg cgcggagtcc actggaccgc cctgaaccag gaggaagatc
     4321 gctggctcta tgcagccgag acatgggtgc gagatgcggc ttacgggatt gagcccaggc
     4381 tcaagaccaa actctctcca gagaatagtt tgttacgcac gcttcttgtg cgaaccgctt
     4441 acttctacct gcggagtctt cgagatcaga tcaaaccctc tgagctttta cttatgagca
     4501 aacatcggcg gcacatgatg aagtgggtca gggagcatct atttccccag atcgaagccg
     4561 gcaagcaccc cgatattcaa gcagagtggt ccaacgacac tctcgaggac gtggagcaat
     4621 ggagcgccag ctatttggct tcaggaaaca atgatatgca gctactacat gccgtgggtg
     4681 gtaagctgcc agccatagct cgcggaacta ttcctcctct ccaggtactg atgaaggacg
     4741 gtatgctcga ccgtttgtat gtggagggag tcgggtttgc tgacggcaac ctcgatctcg
     4801 agtccttggt taagcagctt gcacatcggt accctcgtat gaaggtgctc gaagtcggtg
     4861 ctgggacggg tggtactact cgagcggtgc tttcggcttt ggacggcaaa tacacatcat
     4921 atacgtacac ggatatctcg gccggcttct ttgagcctgc gaaagcaaag ttcagccagc
     4981 atctgagtca gctgtcgttc aagatgctca acatcgaaaa ggacccggta gagcaaggat
     5041 tcgaggaggg ctcgttcgat atggtcgttg cctcgaactg cctccacgca acacggagcc
     5101 tgaaggatac cgtccgccac tgtcgccgct tacttcgacc tggtgggtac ttggtgctgc
     5161 tggagatcac acgcgaccat cttccaatcc agctcatcat gggtacgctt cctgggtggt
     5221 tcctgggcgc cgatgaggga cgagtctggg ctcccacgat cggcctcgac gagtgggatg
     5281 ctttactgaa ggtcaccggg ttttcagggg tggacacaag ctcgacgcct tccttctgtt
     5341 cggtcatctt atcccaggca accgacgaaa ccatccagat gctccgtcag cccctttcca
     5401 ccaattctca gtcgccaatg ccgcctctcg accaacaagt cctggtcgtc ggcggttctg
     5461 aactcgcatc caggtcgcaa acactcctcg cttctgccgg ggctgtcatc agacgtaacc
     5521 ttgagggcat tgagattccc agcggtgccg ttgtgctctg cctttgtgac ctggacagcc
     5581 cggtctttaa ctccatgagc cagtcgaggt tcgaagccgt gcaggccgtc ttccgaaacg
     5641 ccagtactgt tctatgggtg agttcagggg cgacttctgg caagaatccc ctggccaacg
     5701 tgaccgtggg attgggacgg actcttctgg ccgaacgtag tgacctgagg cttcagttcc
     5761 tcgacgtcga tgcgcccgca acccttgaac cgtctttact agcaactctt ctcctccgac
     5821 ttaccgggat gaattcagac tacaattcgg acgagatcct ctggacccct gagccccagc
     5881 ttacactaag ggacggtgct atatatatac ccagggttct ggctctggat gctatcaatc
     5941 ttcggtccac ggcgaggcac cgacaagtca cccagctgac cagctttgag cttccagata
     6001 tagtcatcga ggtcacgaag cgacacggag cactcgaact gctgtatcgc catcaccatg
     6061 agcatgtcca caatgttaag ccaggcgagg tgcgtgtgaa ggtcacggca tcgtctcttt
     6121 actcccttgt ctgctgcgaa ggttcgtcca agatgtctgc atctgcatct tcgaggcctg
     6181 ctcatctgtg ggtgggccac gacatggtat caggggataa actcgtcggc ctttcacacg
     6241 caagcagctc catcgtcacg gttgctgagg attgcgtctt gtatcgccaa cgcagtgccg
     6301 ccaacaccgc cgccgaggag tcggtcgacg ccgcgcaaca gctccaccat ttcacagctc
     6361 aggcgctagt gaagcatctc cttcgcgact tggagggccc cgtctggatt cacggcgccc
     6421 caaatcatct cagcaaggca atttcccgtg ttgctgcaga gcagtgtctc accgtgttcg
     6481 agacgacgtc ggatatggag agagctggcg actggggttt catccatccc tacatcagca
     6541 agcgaaatct tcaatcacta cgcatgcgca tgagagacgt gaaaacgttc atcgacttga
     6601 agccctggaa gagcaacagt atttcggcgc tgctgcgtgc ctcgtctcca cgttccaggc
     6661 tcgttaccat cgacatcgaa gatctgaatg atctgattat tggattgagc cgcgaggacc
     6721 tatgcaggct ggccagacag cactttgaag accatgacaa catccggagg ggcccggata
     6781 acctagacca gatcctgccc atcgaaaacg taacaacgga ggtagtcaag gggttgggcc
     6841 ctgcaacggt gattgactgg tccgctaccg acaaagtcac caccgtggtc catcctgtcg
     6901 agcacaacgg gctctttgcc gctaacaaga catatcttct cttcggcatg acgggagatg
     6961 taggcatttc aattgctcta tggatggtgg atcacggagc tcggagcgtc gtcctggcca
     7021 gccgtaagcc cagtgtgcca gccagtgtgg ttgagcatat gtcgcaaaag ggggcagacc
     7081 tgcgtgtcat ggccgttgac attacgaaca gagatgctct gagaaccttt tatgccgaca
     7141 tcaagtccag catgccgccg gtcggcggcg tcatcaatgg tgcgatggtg cttcgggacc
     7201 gtctctttgt cgacatgtcc tggcccgact ttgaggccgt gctggcgccc aaggttgtgg
     7261 gaactcagaa tattgatgaa ctctttggcg acggtgaaga ggatgagact ctggacttct
     7321 tcatcgtcct ctcgtcggcg acttcgctgg tcggcattat cggccaatcc gcctacagtg
     7381 ctgcgaacca cttcgcggcc agcctggtgc gccagcgtcg cagtcgtggc ctggcgggtt
     7441 ctgtcatcgt cattgggttc ctgacggggc tgggctacat cttccatcgt tcagacaagg
     7501 aacacctcgc gacgattgaa aagtcacttc tgccacggct ggatagacaa tccgagacag
     7561 atctgtgcga gatgctggcc gaggccattg tgtgcggacg acccaactca gaccagccac
     7621 ccgagctaat cacaggtata aggacgtcct tccaagaggc atggcaccaa gacccgcggc
     7681 tttcgtgcta tctcgtccaa gaaggagttc gagagagcgg cggtggacca gaggaggtca
     7741 acgggaacgt taaagtcgag gttcagctgg cggcggtgga ggatcccaaa gacgggctga
     7801 cagtcttgga gaagtgcttt tcccaggctt tgggcaacat gctccagctc gacccagcgc
     7861 aaatatcggg cgatgttcct gcagtcaacc ttggagtcga ctccctcgtc tcggtccgga
     7921 ttcgtgagtg gttcctgaag gagctcggcg tcgacgtacc catcctgaaa attatgtcca
     7981 cgaaccacac tgtatctcga ctgtgtgagg acgctctgac aggctggcga aggcttcagg
     8041 cagggtccga tatggcggcg gcaacatcct cttccggcac agctcgtcag gacccagaga
     8101 tggactggac aaaggaattg accggactta ttgacggaat ccctgctctt atcccgcaag
     8161 gagccgagtc ggtcaaggat gcgcctcgga gaagtgcacg gcgcatcgtt ctcacagggt
     8221 gcacaggctt cttgggtacg cacatgcttc gcaacctggt cgcagatgac aacgttgctg
     8281 agctgcactg tctctgcatt cgctcacgcc gtgttcgcgt ccgggacccc aggattcgag
     8341 agtacaaagg cgacctgatg aagcccctgt tggggctttc taacgacgac ttcatccgcc
     8401 tcagccagac tgccgacttg atcatccact tgggcgccga ggtcaaccat ctcaagtcgt
     8461 acgaggctgt acgcaccgcc aatgttgtct cgacacaagt tctcctcgcc atggccacgc
     8521 cgcgcggtgt ccccgtccat ttcgtatcgt catcatcggt cgccatgctg cagaagggga
     8581 cccatgagct gcccgagata cccccgtcag ccatctcccc tccgaccgat gccgagtccc
     8641 tgatgaagaa tgccataggc tacgctgcca gtaagtggat gggcgagatg ctgctagaac
     8701 acgccgcgcc gcctgcggtt gtgcaccgct tccccaacat catggggccc gatgctcccg
     8761 atgagatccc gctagttgcg ctggaccgct tctgcacccg gatgcgagcc gtgccggcgt
     8821 tggatccgca gcagtgggta ggacaactgg atatcatcga ggtcagtgat gtcgtgcccg
     8881 agtttttggc aaacgcatat gggcacgatc cccagaaggg ttttgccgtg cacaactatt
     8941 gcagcagtaa cgagtactgg ctgtcggacc tggcgggcat gtatgagaaa aagctcggcg
     9001 gccacattaa agtcttgccg acggccgagt ggatgcggag agcgacggcc atggggatgc
     9061 cgaagggagt agtggcgacg tttactggcc atgacgaagt cttcgtatcg ccagtattgc
     9121 ggaaggggtc aggttcgagg taaggagatt gataagactt ttctagttgc atatctttta
     9181 tatttaaatc ttatctatta gttaattttt tgtaatttat ccttatatat agtctggtta
     9241 ttctaaaata tcatttcagt atctaaaaat tcccctcttt tttcagttat atcttaacag
     9301 gcgacagtcc aaatgttgat ttatcccagt ccgattcatc agggttgtga agcattttgt
     9361 caatggtcga aatcacatca gtaatagtgc ctcttacttg cctcatagaa tttctttctc
     9421 ttaacgtcac cgtttggtct tttatagttt cgaaatctat ggtgatacca aatggtgttc
     9481 ccaattcatc gttacgggcg tattttttac caattgaagt attggaatcg tcaattttaa
     9541 agtatatctc tcttttacgt aaagcctgcg agatcctctt aagtatagcg gggaagccat
     9601 cgttattcga tattgtcgta acaaatactt tgatcggcgc tatgcggccg ccaccgcggt
     9661 ggagctccag cttttgttcc ctttagtgag ggttaattgc gcgcttggcg agactcaaac
     9721 aagatatatt ttgcccgcct tacagacgaa actagttgga ggttatggag catactatca
     9781 cgtgggcggc cactggtgag ttactacacc ccaggggcaa cgttgatgct cctaaaaaac
     9841 tctggctgga cgcaagccgt aacacccgtg tcacttcata atcgtttgca attcagggct
     9901 tgatctacac attaccctgt tatccctatt aattaatacg gttatccaca gaatcagggg
     9961 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
    10021 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
    10081 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
    10141 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
    10201 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
    10261 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
    10321 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
    10381 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
    10441 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
    10501 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
    10561 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
    10621 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
    10681 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
    10741 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
    10801 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
    10861 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
    10921 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
    10981 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
    11041 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
    11101 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
    11161 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
    11221 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
    11281 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
    11341 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
    11401 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
    11461 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
    11521 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
    11581 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
    11641 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
    11701 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
    11761 gcacatttcc ccgaaaagtg ccacctgaac gaagcatctg tgcttcattt tgtagaacaa
    11821 aaatgcaacg cgagagcgct aatttttcaa acaaagaatc tgagctgcat ttttacagaa
    11881 cagaaatgca acgcgaaagc gctattttac caacgaagaa tctgtgcttc atttttgtaa
    11941 aacaaaaatg caacgcgaga gcgctaattt ttcaaacaaa gaatctgagc tgcattttta
    12001 cagaacagaa atgcaacgcg agagcgctat tttaccaaca aagaatctat acttcttttt
    12061 tgttctacaa aaatgcatcc cgagagcgct atttttctaa caaagcatct tagattactt
    12121 tttttctcct ttgtgcgctc tataatgcag tctcttgata actttttgca ctgtaggtcc
    12181 gttaaggtta gaagaaggct actttggtgt ctattttctc ttccataaaa aaagcctgac
    12241 tccacttccc gcgtttactg attactagcg aagctgcggg tgcatttttt caagataaag
    12301 gcatccccga ttatattcta taccgatgtg gattgcgcat actttgtgaa cagaaagtga
    12361 tagcgttgat gattcttcat tggtcagaaa attatgaacg gtttcttcta ttttgtctct
    12421 atatactacg tataggaaat gtttacattt tcgtattgtt ttcgattcac tctatgaata
    12481 gttcttacta caattttttt gtctaaagag taatactaga gataaacata aaaaatgtag
    12541 aggtcgagtt tagatgcaag ttcaaggagc gaaaggtgga tgggtaggtt atatagggat
    12601 atagcacaga gatatatagc aaagagatac ttttgagcaa tgtttgtgga agcggtattc
    12661 gcaatatttt agtagctcgt tacagtccgg tgcgtttttg gttttttgaa agtgcgtctt
    12721 cagagcgctt ttggttttca aaagcgctct gaagttccta tactttctag agaataggaa
    12781 cttcggaata ggaacttcaa agcgtttccg aaaacgagcg cttccgaaaa tgcaacgcga
    12841 gctgcgcaca tacagctcac tgttcacgtc gcacctatat ctgcgtgttg cctgtatata
    12901 tatatacatg agaagaacgg catagtgcgt gtttatgctt aaatgcgtac ttatatgcgt
    12961 ctatttatgt aggatgaaag gtagtctagt acctcctgtg atattatccc attccatgcg
    13021 gggtatcgta tgcttccttc agcactaccc tttagctgtt ctatatgctg ccactcctca
    13081 attggattag tctcatcctt caatgctatc atttcctttg atattggatc atactaagaa
    13141 accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctc
    13201 gcgcgtttcg gtgatgacgg tgagcgcgtt tcggtgatga cggtgaaaac ctcggcgcgc
    13261 cattaccctg ttatccctaa tatacaacgc aaagaagctg gaaaattggc gagggaatcc
    13321 tgtttctgtc tatccaagaa tgggcatgag gtggcaaccg tcgtgctagc gtacagggtg
    13381 cactttgtaa ccatttggga caccggacac tcgctgtttt cgaaattacc ctttaagcgc
    13441 gggtattgaa ccaggcttat gcccaagatc gtagcaagca tttaactgtg ggaatactca
    13501 ggtatcgtaa gatgcaagag ttcgaatctc ttagcaacca ttattttttt cctcaacata
    13561 acgagaacac acaggggcgc tatcgcacag aatcaaattc gatgactgga aattttttgt
    13621 taatttcaga ggtcgcctga cgcatatacc tttttcaact gaaaaattgg gagaaaaagg
    13681 aaaggtgaga gcgccggaac cggcttttca tatagaatag agaagcgttc atgactaaat
    13741 gcttgcatca caatacttga agttgacaat attatttaag gacctattgt tttttccaat
    13801 aggtggttag caatcgtctt actttctaac ttttcttacc ttttacattt cagcaatata
    13861 tatatatata tttcaaggat ataccattct aatgtctgcc cctaagaaga tcgtcgtttt
    13921 gccaggtgac cacgttggtc aagaaatcac agccgaagcc attaaggttc ttaaagctat
    13981 ttctgatgtt cgttccaatg tcaagttcga tttcgaaaat catttaattg gtggtgctgc
    14041 tatcgatgct acaggtgttc cacttccaga tgaggcgctg gaagcctcca agaaggctga
    14101 tgccgttttg ttaggtgctg tgggtggtcc taaatggggt accggtagtg ttagacctga
    14161 acaaggttta ctaaaaatcc gtaaagaact tcaattgtac gccaacttaa gaccatgtaa
    14221 ctttgcatcc gactctcttt tagacttatc tccaatcaag ccacaatttg ctaaaggtac
    14281 tgacttcgtt gttgtcagag aattagtggg aggtatttac tttggtaaga gaaaggaaga
    14341 cgatggtgat ggtgtcgctt gggatagtga acaatacacc gttccagaag tgcaaagaat
    14401 cacaagaatg gccgctttca tggccctaca acatgagcca ccattgccta tttggtcctt
    14461 ggataaagct aatgttttgg cctcttcaag attatggaga aaaactgtgg aggaaaccat
    14521 caagaacgaa ttccctacat tgaaggttca acatcaattg attgattctg ccgccatgat
    14581 cctagttaag aacccaaccc acctaaatgg tattataatc accagcaaca tgtttggtga
    14641 tatcatctcc gatgaagcct ccgttatccc aggttccttg ggtttgttgc catctgcgtc
    14701 cttggcctct ttgccagaca agaacaccgc atttggtttg tacgaaccat gccacggttc
    14761 tgctccagat ttgccaaaga ataaggtcaa ccctatcgcc actatcttgt ctgctgcaat
    14821 gatgttgaaa ttgtcattga acttgcctga agaaggtaag gccattgaag atgcagttaa
    14881 aaaggttttg gatgcaggta tcagaactgg tgatttaggt ggttccaaca gtaccaccga
    14941 agtcggtgat gctgtcgccg aagaagttaa gaaaatcctt gcttaaaaag attctctttt
    15001 tttatgatat ttgtacataa actttataaa tgaaattcat aatagaaacg acacgaaatt
    15061 acaaaatgga atatgttcat agggtagacg aaactatata cgcaatctac atacatttat
    15121 caagaaggag aaaaaggagg atgtaaagga atacaggtaa gcaaattgat actaatggct
    15181 caacgaaatg acggtatcga taagcttgat atcgaattcc tgcagcccgg gg
//
