LOCUS       sequence-274350-        9149 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tet-regulated miR-E (miR-30 variant)-based RNAi.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9149)
  AUTHORS   Fellmann C, Hoffmann T, Sridhar V, Hopfgartner B, Muhar M, Roth M, 
            Lai DY, Barbosa IA, Kwon JS, Guan Y, Sinha N, Zuber J
  TITLE     An optimized microRNA backbone for effective single-copy RNAi.
  JOURNAL   Cell Rep. 2013 Dec 26;5(6):1704-13. doi: 
            10.1016/j.celrep.2013.11.020. Epub 2013 Dec 12.
  PUBMED    24332856
REFERENCE   2  (bases 1 to 9149)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2013.11.020"; journalName: "Cell Rep"; date: 
            "2013-12-26- 26"; volume: "5"; issue: "6"; pages: "1704-13"
COMMENT     Sequence Label: RT3REVIN
FEATURES             Location/Qualifiers
     source          1..9149
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(43..62)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     436..459
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     ncRNA           complement(673..687)
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     CDS             complement(871..1548)
                     /codon_start=1
                     /product="improved tetrameric variant of DsRed fluorescent 
                     protein"
                     /label=DsRed2
                     /note="mammalian codon-optimized"
                     /translation="MASSENVITEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTVK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     ATVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGETHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDAKLDITSHNEDYTIVEQYERTEGRHH
                     LFL"
     primer_bind     complement(933..956)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     1348..1368
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     promoter        complement(1572..1950)
                     /label=TRE3G promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G"
     primer_bind     complement(1639..1663)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    1708..1726
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1744..1762
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1780..1798
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1816..1834
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1852..1870
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1888..1906
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1924..1942
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             complement(1987..2403)
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     complement(2003..2019)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(2035..2057)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     misc_feature    2604..2803
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     LTR             2866..3041
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     promoter        complement(3042..3245)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(3071..3091)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        3246..3625
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     complement(3725..3743)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3811..3915
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3916..3984,3985..4776)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4134..4153)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4947..5535
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5436..5455
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5689..5706
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(5787..5806)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        complement(5828..6157)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5842..5977
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6142..6162
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     primer_bind     6298..6320
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     LTR             6423..6848
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     Moloney murine leukemia virus"
     CDS             complement(6987..7781)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(6987..7781)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7098..7117)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     7708..7727
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     misc_feature    7797..8370
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7957..7976)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     8186..8203
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     CDS             complement(join(8402..9115,9116..9118,9119..9121))
                     /codon_start=1
                     /product="yellow fluorescent protein (YFP) with fast and 
                     efficient maturation (Nagai et al., 2002)"
                     /label=Venus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(8447..8468)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     8796..8815
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     9055..9076
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     promoter        complement(join(9144..9149,1..494))
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
ORIGIN
        1 gcccggagat gaggaagagg agaacagcgc ggcagacgtg cgcttttgaa gcgtgcagaa
       61 tgccgggcct ccggaggacc ttcgggcgcc cgccccgccc ctgagcccgc ccctgagccc
      121 gcccccggac ccaccccttc ccagcctctg agcccagaaa gcgaaggagc aaagctgcta
      181 ttggccgctg ccccaaaggc ctacccgctt ccattgctca gcggtgctgt ccatctgcac
      241 gagactagtg agacgtgcta cttccatttg tcacgtcctg cacgacgcga gctgcggggc
      301 gggggggaac ttcctgacta ggggaggagt agaaggtggc gcgaaggggc caccaaagaa
      361 cggagccggt tggcgcctac cggtggatgt ggaatgtgtg cgaggccaga ggccacttgt
      421 gtagcgccaa gtgcccagcg gggctgctaa agcgcatgct ccagactgcc ttgggaaaag
      481 cgcctcccct acccggtaga attacgcgtc aattgaaaaa agtgatttaa tttataccat
      541 tttaattcag ctttgtaaaa atgtatcaaa gagatagcaa ggtattcagt tttagtaaac
      601 aagataattg ctcgaattct agccccttga agtccgaggc agtaggcata ggaattataa
      661 tgcttatcta tacatctgtg gcttcactat agataagcat tataattcct gcgctcactg
      721 tcaacagcaa tataccttct cgagccttct gttgggttaa gaagtcgaag taatcccagc
      781 aagtgtttcc aagatgtgca ggcaacgatt ctgtaaagta ctgaagcctc attcaaacaa
      841 ttaccctgtt atccctagtc gaggcggccg ctacaggaac aggtggtggc ggccctcggt
      901 gcgctcgtac tgctccacga tggtgtagtc ctcgttgtgg gaggtgatgt ccagcttggc
      961 gtccacgtag tagtagccgg gcagctgcac gggcttcttg gccatgtaga tggacttgaa
     1021 ctccaccagg tagtggccgc cgtccttcag cttcagggcc ttgtgggtct cgcccttcag
     1081 cacgccgtcg cgggggtaca ggcgctcggt ggaggcctcc cagcccatgg tcttcttctg
     1141 catcacgggg ccgtcggagg ggaagttcac gccgatgaac ttcaccttgt agatgaagca
     1201 gccgtcctgc agggaggagt cctgggtcac ggtcgccacg ccgccgtcct cgaagttcat
     1261 cacgcgctcc cacttgaagc cctcggggaa ggacagcttc ttgtagtcgg ggatgtcggc
     1321 ggggtgcttc acgtacacct tggagccgta ctggaactgg ggggacagga tgtcccaggc
     1381 gaagggcagg gggccgccct tggtcacctt cagcttcacg gtgttgtggc cctcgtaggg
     1441 gcggccctcg ccctcgccct cgatctcgaa ctcgtggccg ttcacggtgc cctccatgcg
     1501 caccttgaag cgcatgaact cggtgatgac gttctcggag gaggccatgg tggatccaac
     1561 gcaagctcga ctttacgagg gtaggaagtg gtacggaaag ttggtataag acaaaagtgt
     1621 tgtggaattg ctccaggcga tctgacggtt cactaaacga gctctgcttt tataggcgcc
     1681 caccgtacac gcctaaagct tatacgttct ctatcactga tagggagtaa actggatata
     1741 cgttctctat cactgatagg gagtaaactg tagatacgtt ctctatcact gatagggagt
     1801 aaactggtca tacgttctct atcactgata gggagtaaac tccttatacg ttctctatca
     1861 ctgataggga gtaaagtctg catacgttct ctatcactga tagggagtaa actcttcata
     1921 cgttctctat cactgatagg gagtaaactc gaatctagag gatcgatccc ccagatcttc
     1981 aattccggcg cctagagaag gagtgagggc tggataaagg gaggatcgag gcggggtcga
     2041 acgaggaggt tcaaggggga gagacggggc ggatggagga agaggaggcg gaggcttagg
     2101 gtgtacaaag ggcttgaccc agggaggggg gtcaaaagcc aaggcttccc aggtcacgat
     2161 gtaggggacc tggtctgggt gtccatgcgg gccaggtgaa aagaccttga tcttaacctg
     2221 ggtgatgagg tctcggttaa aggtgccgtc tcgcggccat ccgacgttaa aggttggcca
     2281 ttctgcagag cagaaggtaa cccaacgtct cttcttgaca tctaccgact ggttgtgagc
     2341 gatccgctcg acatctttcc agtgacctaa ggtcaaactt aagggagtgg taacagtctg
     2401 gccctaattt tcagacaaat acagaaacac agtcagacag agacaacaca gaacgatgct
     2461 gcagcgctgc agcagacaag acgcgcggct tcggttccaa accgaaagca aaaattcaga
     2521 cggaggcggg aactgtttta ggttctcgtc tcctaccaga accacatatc ctgacggggt
     2581 cggattccac atcgactccc ttcctcaggt cgggccacaa aaacggcccc caaagtccct
     2641 gggacgtctc ccagggttgc ggccgggtgt tcagaactcg tcagttccac cacgggtccg
     2701 ccagatacag agctagttag ctaactagta cagacgcagg cgcataacat caaacataga
     2761 cactagacaa tcggacagac acagataagt tgctggccag cttacctccc ggtggtgggt
     2821 cggtggtccc tgggcagggg tctccaaatc ccggacgagc ccccaaatga aagacccccg
     2881 tcgtgggtag tcaatcactc agaggagacc ctcccaagga acagcgagac cacgattcgg
     2941 atgcaaacag caagaggctt tattgggaat acgggtaccc gggcgacgca gtctatcgga
     3001 agactggcgc gccgagtgag gggttgtggg ctcttttatt gagctctgct tatatagacc
     3061 tcccaccgta cacgcctacc gcccatttgc gtcaatgggg cggagttgtt acgacatttt
     3121 ggaaagtccc gttgattttg gtgccaaaac aaactcccat tgacgtcaat ggggtggaga
     3181 cttggaaatc cccgtgagtc aaaccgctat ccacgcccat tgatgtactg ccaaaaccgc
     3241 atcaccatgg taatagcgat gactaatacg tagatgtact gccaagtagg aaagtcccat
     3301 aaggtcatgt actgggcata atgccaggcg ggccatttac cgtcattgac gtcaataggg
     3361 ggcgtacttg gcatatgata cacttgatgt actgccaagt gggcagttta ccgtaaatac
     3421 tccacccatt gacgtcaatg gaaagtccct attggcgtta ctatgggaac atacgtcatt
     3481 attgacgtca atgggcgggg gtcgttgggc ggtcagccag gcgggccatt taccgtaagt
     3541 tatgtaacgc ggaactccat atatgggcta tgaactaatg accccgtaat tgattactat
     3601 taataactag tcaataatca atgtcaacat ggcggtaatg ttggacatga gccaatataa
     3661 atgtacatat tatgatatgg atacaacgta tgcaatggcc aagctaattc ttgaagacga
     3721 aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag
     3781 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     3841 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     3901 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     3961 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     4021 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     4081 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     4141 ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat
     4201 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     4261 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     4321 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     4381 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     4441 cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa
     4501 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     4561 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     4621 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     4681 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     4741 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     4801 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     4861 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     4921 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     4981 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     5041 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta
     5101 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     5161 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     5221 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     5281 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     5341 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     5401 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     5461 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     5521 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     5581 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     5641 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     5701 agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt
     5761 tcacaccgca tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag
     5821 taagcttttt gcaaaagcct aggcctccaa aaaagcctcc tcactacttc tggaatagct
     5881 cagaggccga ggcggcctcg gcctctgcat aaataaaaaa aattagtcag ccatggggcg
     5941 gagaatgggc ggaactgggc ggagttaggg gcgggatggg cggagttagg ggcgggacta
     6001 tggttgctga ctaattgaga tgcatgcttt gcatacttct gcctgctggg gagcctgggg
     6061 actttccaca cctggttgct gactaattga gatgcatgct ttgcatactt ctgcctgctg
     6121 gggagcctgg ggactttcca caccctaact gacacacatt ccacagccgg atctacactc
     6181 cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg
     6241 cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg
     6301 ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagccagctt
     6361 acctcccggt ggtgggtcgg tggtccctgg gcaggggtct cccgatcccg gacgagcccc
     6421 caaatgaaag acccccgctg acgggtagtc aatcactcag aggagaccct cccaaggaac
     6481 agcgagacca caagtcggat gcaactgcaa gagggtttat tggatacacg ggtacccggg
     6541 cgactcagtc aatcggagga ctggcgcccc gagtgagggg ttgtgggctc ttttattgag
     6601 ctcggggagc agaagcgcgc gaacagaagc gagaagcgaa ctgattggtt agttcaaata
     6661 aggcacaggg tcatttcagg tccttggggc accctggaaa catctgatgg ttctctaggg
     6721 tcgaccctgt tccatctgtt cctgaccttg atctgaactt ctctattctc agttatgtat
     6781 ttttccatgc cttgcaaaat ggcgttactt aagctagctt gccaaaccta caggtggggt
     6841 ctttcattcc cccctttttc tggagactaa ataaaatctt ttattttatc tatggctcgt
     6901 actctatagg cttcagctgg tgatattgtt gagtcaaaac tagagcctgg accactgata
     6961 tctcgacggt atcgatgcat cgtacatcag aagaactcgt caagaaggcg atagaaggcg
     7021 atgcgctgcg aatcgggagc ggcgataccg taaagcacga ggaagcggtc agcccattcg
     7081 ccgccaagct cttcagcaat atcacgggta gccaacgcta tgtcctgata gcggtccgcc
     7141 acacccagcc ggccacagtc gatgaatcca gaaaagcggc cattttccac catgatattc
     7201 ggcaagcagg catcgccatg ggtcacgacg agatcctcgc cgtcgggcat gctcgccttg
     7261 agcctggcga acagttcggc tggcgcgagc ccctgatgct cttcgtccag atcatcctga
     7321 tcgacaagac cggcttccat ccgagtacgt gctcgctcga tgcgatgttt cgcttggtgg
     7381 tcgaatgggc aggtagccgg atcaagcgta tgcagccgcc gcattgcatc agccatgatg
     7441 gatactttct cggcaggagc aaggtgagat gacaggagat cctgccccgg cacttcgccc
     7501 aatagcagcc agtcccttcc cgcttcagtg acaacgtcga gcacagctgc gcaaggaacg
     7561 cccgtcgtgg ccagccacga tagccgcgct gcctcgtctt gcagttcatt cagggcaccg
     7621 gacaggtcgg tcttgacaaa aagaaccggg cgcccctgcg ctgacagccg gaacacggcg
     7681 gcatcagagc agccgattgt ctgttgtgcc cagtcatagc cgaatagcct ctccacccaa
     7741 gcggccggag aacctgcgtg caatccatct tgttcaatca tggtggaccg gtaagcttat
     7801 catcgtgttt ttcaaaggaa aaccacgtcc ccgtggttcg gggggcctag acgttttttt
     7861 aacctcgact aaacacatgt aaagcatgtg caccgaggcc ccagatcaga tcccatacaa
     7921 tggggtacct tctgggcatc cttcagcccc ttgttgaata cgcttgagga gagccatttg
     7981 actctttcca caactatcca actcacaacg tggcactggg gttgtgccgc ctttgcaggt
     8041 gtatcttata cacgtggctt ttggccgcag aggcacctgt cgccaggtgg ggggttccgc
     8101 tgcctgcaaa gggtcgctac agacgttgtt tgtcttcaag aagcttccag aggaactgct
     8161 tccttcacga cattcaacag accttgcatt cctttggcga gaggggaaag acccctagga
     8221 atgctcgtca agaagacagg gccaggtttc cgggccctca cattgccaaa agacggcaat
     8281 atggtggaaa ataacatata gacaaacgca caccggcctt attccaagcg gcttcggcca
     8341 gtaacgttag ggggggggga gggagagggg ggggggggag aggggcggaa ttgaacaatt
     8401 cttacttgta cagctcgtcc atgccgagag tgatcccggc ggcggtcacg aactccagca
     8461 ggaccatgtg atcgcgcttc tcgttggggt ctttgctcag ggcggactgg tagctcaggt
     8521 agtggttgtc gggcagcagc acggggccgt cgccgatggg ggtgttctgc tggtagtggt
     8581 cggcgagctg cacgccgccg tcctcgatgt tgtggcggat cttgaagttg gccttgatgc
     8641 cgttcttctg cttgtcggcg gtgatataga cgttgtggct gttgtagttg tactccagct
     8701 tgtgccccag gatgttgccg tcctccttga agtcgatgcc cttcagctcg atgcggttca
     8761 ccagggtgtc gccctcgaac ttcacctcgg cgcgggtctt gtagttgccg tcgtccttga
     8821 agaagatggt gcgctcctgg acgtagcctt cgggcatggc ggacttgaag aagtcgtgct
     8881 gcttcatgtg gtcggggtag cgggcgaagc actgcaggcc gtagcccagg gtggtcacga
     8941 gggtgggcca gggcacgggc agcttgccgg tggtgcagat cagcttcagg gtcagcttgc
     9001 cgtaggtggc atcgccctcg ccctcgccgg acacgctgaa cttgtggccg tttacgtcgc
     9061 cgtccagctc gaccaggatg ggcaccaccc cggtgaacag ctcctcgccc ttgctcacca
     9121 tggtggaagc ttgggctgca ggtcgaaag
//
