LOCUS       sequence-274361-        5778 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Part Plasmid for dCas9, Part 3b(coding sequence).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5778)
  AUTHORS   Geller SH, Antwi EB, Di Ventura B, McClean MN
  TITLE     Optogenetic Repressors of Gene Expression in Yeasts Using 
            Light-Controlled Nuclear Localization.
  JOURNAL   Cell Mol Bioeng. 2019 Sep 24;12(5):511-528. doi: 
            10.1007/s12195-019-00598-9. eCollection 2019 Oct.
  PUBMED    31719930
REFERENCE   2  (bases 1 to 5778)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1007/s12195-019-00598-9"; journalName: "Cell Mol Bioeng"; date: 
            "2019-09-24- 24"; volume: "12"; issue: "5"; pages: "511-528"
COMMENT     Sequence Label: pMM587
FEATURES             Location/Qualifiers
     source          1..5778
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(4194..4228)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(4299..4958)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     4873..4892
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      5140..5728
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5629..5648
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta gtcggtctca
       61 ttctgacaag aagtattcta tcggactggc catcgggact aatagcgtcg ggtgggccgt
      121 gatcactgac gagtacaagg tgccctctaa gaagttcaag gtgctcggga acaccgaccg
      181 gcattccatc aagaaaaatc tgatcggagc tctcctcttt gattcagggg aaaccgctga
      241 agcaacccgc ctcaagcgga ctgctagacg gcggtacacc aggaggaaga accggatttg
      301 ttaccttcaa gagatattct ccaacgaaat ggcaaaggtc gacgacagct tcttccatag
      361 gctggaagaa tcattcctcg tggaagagga taagaagcat gaacggcatc ccatcttcgg
      421 taatatcgtc gacgaggtgg cctatcacga gaaataccca accatctacc atcttcgcaa
      481 aaagctggtg gactcaaccg acaaggcaga cctccggctt atctacctgg ccctggccca
      541 catgatcaag ttcagaggcc acttcctgat cgagggcgac ctcaatcctg acaatagcga
      601 tgtggataaa ctgttcatcc agctggtgca gacttacaac cagctctttg aagagaaccc
      661 catcaatgca agcggagtcg atgccaaggc cattctgtca gcccggctgt caaagagccg
      721 cagacttgag aatcttatcg ctcagctgcc gggtgaaaag aaaaatggac tgttcgggaa
      781 cctgattgct ctttcacttg ggctgactcc caatttcaag tctaatttcg acctggcaga
      841 ggatgccaag ctgcaactgt ccaaggacac ctatgatgac gatctcgaca acctcctggc
      901 ccagatcggt gaccaatacg ccgacctttt ccttgctgct aagaatcttt ctgacgccat
      961 cctgctgtct gacattctcc gcgtgaacac tgaaatcacc aaggcccctc tttcagcttc
     1021 aatgattaag cggtatgatg agcaccacca ggacctgacc ctgcttaagg cactcgtccg
     1081 gcagcagctt ccggagaagt acaaggaaat cttctttgac cagtcaaaga atggatacgc
     1141 cggctacatc gacggaggtg cctcccaaga ggaattttat aagtttatca aacctatcct
     1201 tgagaagatg gacggcaccg aagagctcct cgtgaaactg aatcgggagg atctgctgcg
     1261 gaagcagcgc actttcgaca atgggagcat tccccaccag atccatcttg gggagcttca
     1321 cgccatcctt cggcgccaag aggacttcta cccctttctt aaggacaaca gggagaagat
     1381 tgagaaaatt ctcactttcc gcatccccta ctacgtggga cccctcgcca gaggaaatag
     1441 ccggtttgct tggatgacca gaaagtcaga agaaactatc actccctgga acttcgaaga
     1501 ggtggtggac aagggagcca gcgctcagtc attcatcgaa cggatgacta acttcgataa
     1561 gaacctcccc aatgagaagg tcctgccgaa acattccctg ctctacgagt actttaccgt
     1621 gtacaacgag ctgaccaagg tgaaatatgt caccgaaggg atgaggaagc ccgcattcct
     1681 gtcaggcgaa caaaagaagg caattgtgga ccttctgttc aagaccaata gaaaggtgac
     1741 cgtgaagcag ctgaaggagg actatttcaa gaaaattgaa tgcttcgact ctgtggagat
     1801 tagcggggtc gaagatcggt tcaacgcaag cctgggtacc taccatgatc tgcttaagat
     1861 catcaaggac aaggattttc tggacaatga ggagaaagag gacatccttg aggacattgt
     1921 cctgactctc actctgttcg aggaccggga aatgatcgag gagaggctta agacctacgc
     1981 ccatctgttc gacgataaag tgatgaagca acttaaacgg agaagatata ccggatgggg
     2041 acgccttagc cgcaaactca tcaacggaat ccgggacaaa cagagcggaa agaccattct
     2101 tgatttcctt aagagcgacg gattcgctaa tcgcaacttc atgcaactta tccatgatga
     2161 ttccctgacc tttaaggagg acatccagaa ggcccaagtg tctggacaag gtgactcact
     2221 gcacgagcat atcgcaaatc tggctggttc acccgctatt aagaagggta ttctccagac
     2281 cgtgaaagtc gtggacgagc tggtcaaggt gatgggtcgc cataaaccag agaacattgt
     2341 catcgagatg gccagggaaa accagactac ccagaaggga cagaagaaca gcagggagcg
     2401 gatgaaaaga attgaggaag ggattaagga gctcgggtca cagatcctta aagagcaccc
     2461 ggtggaaaac acccagcttc agaatgagaa gctctatctg tactaccttc aaaatggacg
     2521 cgatatgtat gtggaccaag agcttgatat caacaggctc tcagactacg acgtggacgc
     2581 catcgtccct cagagcttcc tcaaagacga ctcaattgac aataaggtgc tgactcgctc
     2641 agacaagaac cggggaaagt cagataacgt gccctcagag gaagtcgtga aaaagatgaa
     2701 gaactattgg cgccagcttc tgaacgcaaa gctgatcact cagcggaagt tcgacaatct
     2761 cactaaggct gagaggggcg gactgagcga actggacaaa gcaggattca ttaaacggca
     2821 acttgtggag actcggcaga ttactaaaca tgtagcccaa atccttgact cacgcatgaa
     2881 taccaagtac gacgaaaacg acaaacttat ccgcgaggtg aaggtgatta ccctgaagtc
     2941 caagctggtc agcgatttca gaaaggactt tcaattctac aaagtgcggg agatcaataa
     3001 ctatcatcat gctcatgacg catatctgaa tgccgtggtg ggaaccgccc tgatcaagaa
     3061 gtacccaaag ctggaaagcg agttcgtgta cggagactac aaggtctacg acgtgcgcaa
     3121 gatgattgcc aaatctgagc aggagatcgg aaaggccacc gcaaagtact tcttctacag
     3181 caacatcatg aatttcttca agaccgaaat cacccttgca aacggtgaga tccggaagag
     3241 gccgctcatc gagactaatg gggagactgg cgaaatcgtg tgggacaagg gcagagattt
     3301 cgctaccgtg cgcaaagtgc tttctatgcc tcaagtgaac atcgtgaaga aaaccgaggt
     3361 gcaaaccgga ggcttttcta aggaatcaat cctccccaag cgcaactccg acaagctcat
     3421 tgcaaggaag aaggattggg accctaagaa gtacggcgga ttcgattcac caactgtggc
     3481 ttattctgtc ctggtcgtgg ctaaggtgga aaaaggaaag tctaagaagc tcaagagcgt
     3541 gaaggaactg ctgggtatca ccattatgga gcgcagctcc ttcgagaaga acccaattga
     3601 ctttctcgaa gccaaaggtt acaaggaagt caagaaggac cttatcatca agctcccaaa
     3661 gtatagcctg ttcgaactgg agaatgggcg gaagcggatg ctcgcctccg ctggcgaact
     3721 tcagaagggt aatgagctgg ctctcccctc caagtacgtg aatttcctct accttgcaag
     3781 ccattacgag aagctgaagg ggagccccga ggacaacgag caaaagcaac tgtttgtgga
     3841 gcagcataag cattatctgg acgagatcat tgagcagatt tccgagtttt ctaaacgcgt
     3901 cattctcgct gatgccaacc tcgataaagt ccttagcgca tacaataagc acagagacaa
     3961 accaattcgg gagcaggctg agaatatcat ccacctgttc accctcacca atcttggtgc
     4021 ccctgccgca ttcaagtact tcgacaccac catcgaccgg aaacgctata cctccaccaa
     4081 agaagtgctg gacgccaccc tcatccacca gagcatcacc ggactttacg aaactcggat
     4141 tgacctctca cagctcggag gggatgaggg agctggatcc tgagaccaga ccaataaaaa
     4201 acgcccggcg gcaaccgagc gttctgaaca aatccagatg gagttctgag gtcattactg
     4261 gatctatcaa caggagtcca agcgagctcg atatcaaatt acgccccgcc ctgccactca
     4321 tcgcagtact gttgtaattc attaagcatt ctgccgacat ggaagccatc acaaacggca
     4381 tgatgaacct gaatcgccag cggcatcagc accttgtcgc cttgcgtata atatttgccc
     4441 atggtgaaaa cgggggcgaa gaagttgtcc atattggcca cgtttaaatc aaaactggtg
     4501 aaactcaccc agggattggc tgaaacgaaa aacatattct caataaaccc tttagggaaa
     4561 taggccaggt tttcaccgta acacgccaca tcttgcgaat atatgtgtag aaactgccgg
     4621 aaatcgtcgt ggtattcact ccagagcgat gaaaacgttt cagtttgctc atggaaaacg
     4681 gtgtaacaag ggtgaacact atcccatatc accagctcac cgtctttcat tgccatacga
     4741 aattccggat gagcattcat caggcgggca agaatgtgaa taaaggccgg ataaaacttg
     4801 tgcttatttt tctttacggt ctttaaaaag gccgtaatat ccagctgaac ggtctggtta
     4861 taggtacatt gagcaactga ctgaaatgcc tcaaaatgtt ctttacgatg ccattgggat
     4921 atatcaacgg tggtatatcc agtgattttt ttctccattt tagcttcctt agctcctgaa
     4981 aatctcgata actcaaaaaa tacgcccggt agtgatctta tttcattatg gtgaaagttg
     5041 gaacctctta cgtgcccgat caatcatgac caaaatccct taacgtgagt tttcgttcca
     5101 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     5161 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     5221 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     5281 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     5341 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     5401 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     5461 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     5521 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     5581 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     5641 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     5701 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     5761 ggccttttgc tggccttt
//
