LOCUS       sequence-274372-       13996 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pan-neuronal expression of CaMPARI2 in zebrafish, used to generate 
            the CaMPARI2 zebrafish line at ZIRC (ZL13801).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13996)
  AUTHORS   Moeyaert B, Holt G, Madangopal R, Perez-Alvarez A, Fearey BC, 
            Trojanowski NF, Ledderose J, Zolnik TA, Das A, Patel D, Brown TA, 
            Sachdev RNS, Eickholt BJ, Larkum ME, Turrigiano GG, Dana H, Gee CE, 
            Oertner TG, Hope BT, Schreiter ER
  TITLE     Improved methods for marking active neuron populations.
  JOURNAL   Nat Commun. 2018 Oct 25;9(1):4440. doi: 10.1038/s41467-018-06935-2.
  PUBMED    30361563
REFERENCE   2  (bases 1 to 13996)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-10-25"; volume: "9(1):4440. doi"; pages: " 
            10.1038/s41467-018-06935-2"
COMMENT     Sequence Label: pTol2-HuC(elavl3)-CaMPARI2
FEATURES             Location/Qualifiers
     source          1..13996
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(125..146)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(495..506,507..669)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     CDS             9507..9524
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             9531..9971
                     /codon_start=1
                     /product="calcium-binding protein"
                     /label=calmodulin
                     /translation="DQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEA
                     ELQDMINEVDADGDGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYISAAEL
                     RHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVVMMTAK"
     CDS             10767..10790
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             10809..10835
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             10854..10883
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     polyA_signal    10934..11068
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10958..10977)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     11012..11031
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    join(11507..11694,11695..11706)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(11740..11756)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11740..11756)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(11793..11813)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(11793..11811)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(11832..11848)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11832..11848)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11845..11867)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11856..11872
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11880..11886,11887..11904,11905..11910))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11925..11946
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(12063..12080)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12234..12822)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12314..12333)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12993..13784,13785..13853))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13616..13635
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13854..13958)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata
       61 ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt
      121 tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg
      181 aaaaaccgtc tatcagggcg atggcccacg ctggctaaga actcatcagc ctccccggtc
      241 catctaccca cgtaccaatg caccaattgg ccacaatgac ggctactaca tggtgccatt
      301 ccttcctctt tataggaatg gagactacct cctgtccaac aatgctcttg gatacgagta
      361 cgcctacctg ttggacccag gtcattgcac aacaccagaa atgccctctg atctgcaaaa
      421 gacgtgaata tctgttcaga cacccatatc cactctgttc cacacaggtc agaggtttgt
      481 ccaggagttc ttgacagagg tgtaaaaagt actcaaaaat tttactcaag tgaaagtaca
      541 agtacttagg gaaaatttta ctcaattaaa agtaaaagta tctggctaga atcttacttg
      601 agtaaaagta aaaaagtact ccattaaaat tgtacttgag tattaaggaa gtaaaagtaa
      661 aagcaagaaa gaaaactaga gattcttgtt taagcttccc gggtgtcgac gaattctcga
      721 gctctattcc taaagacctt gggtgaccaa aatcttattt taataaataa aactgtttat
      781 taaaactttt ttgtttcaaa gaaccatatg tatagtgaaa tttataaaaa tatcaatttt
      841 taaaaagctg gtgtactcat ttatgttatg aactctaaaa ccatatactg actgcaagtg
      901 atgatgtata gagtgatgtt tacgagtaaa catatttagt tgtatacatc ctactgagca
      961 cattttgatg tatgaaataa cattacaagc tttatccaaa ttaagccatt ttaaaacact
     1021 gccaattgaa aatacaaatc ctggaaaaaa tcgtctttag cgcagtcatt tgagccatcc
     1081 taatccgtta cctcagacca taataagaag ggataacact agctgtagca atggaacaca
     1141 tctgtttcac acaatcatat ctcctgcgcc ggtgctaagc agattcagcg tgatcataac
     1201 atgctttcca ctcataaatg taaatttaca atttgcacat gtaaaacaga cacttttgag
     1261 atattggata aaaaaacaag agtatattgc ttagtttcat ccaccagtca tccccacagc
     1321 gtttggaagg ccataaaaag tgtctaaaat caatgatcat tgaaagagca caagagagac
     1381 tcttacgctg taatgccact ggggacaaaa gtgacagtct cttaatgggc tcttctggag
     1441 gggctcctga acattaaaaa ttatcagcga aattaccgaa agagcttcaa gcaactggca
     1501 tgcttgatcc tctgcgtcgg ggcggtgaat aggtgcttca gatgccctct tacccacggg
     1561 ctggattcag ctgccccgct accagcggag accccctaat gagcctctgc aattaagttt
     1621 attcatgtta agtgtgaacg gggtgcgtgc ggaactgtgg gcagctaaca gacctgggtt
     1681 ctttgtgcca caagtgctgc ctttattcgg ctcacaaagc agaaaacaac acccgcacct
     1741 ataatggcgc cctcggctgg gtctaagaaa cgtggcgagt tgacagagca gagtgggcgg
     1801 ggttaagaca gactgacagc gggacccatc tccatcctct tattaacgct taacgagtgc
     1861 cttcctcatg caatattcat cgccactaat atcatccaag ctctgagctg agctggccac
     1921 ttatgtaagg caattatgta aaatatcaga cagggcccac actcagaatc tgactggggt
     1981 agagacgcgg gacgagaacc gagagcaaga actgaaagtg aaagtgacca ctaaagggag
     2041 gagaggacag aggggcagga tgtgtcaaga ttaccagaga acacttggcc agaaatgcgc
     2101 aaccattgga gctctccgga ttacccaaag gttaacgagt ttgaacgcct ctgcccactc
     2161 gcccatctct gatggtttcc caagaactcc tcaagcaaaa tatatataat tgtgtgtatt
     2221 atgcacagac acgagaaaat gctgtttttc tgatctgcat tacagcacat ttgcccgcca
     2281 acgacaatac cacccactcg gtacctcgct gactcctgat gcctgatacc tgcgcggtga
     2341 ctgtctacaa tctgcataat caagagaagt tgtgttgaag acgagcgcca cacaaccgtt
     2401 tccacaaggt cacccaaggc cggtgcagat gtaggtgagg tctccataaa cagactgaaa
     2461 taaacacatc ctccgctggg aacaacaacc ccctcacgcc tcatgcattt ccataagcct
     2521 acatgcatct cttccaactt atggagactc gcacctacca acatccgcac aacaaagata
     2581 tacagagcgc gctccctcag gtcaaggccc tgtgggggtc tgtgcagaaa taggtcattt
     2641 gtcacacatc aagtcctggg gcaggagatg cattatagat gagaccaaac agcctgtctc
     2701 ggtgagctct acccactccc tgagactaga aatgggggaa gggagcttga gataacaacc
     2761 gctgcaatca ctgtgtcgat gtttaatatc agcaccaacc gggaacaata aggagatggg
     2821 tgcattcatg ttcacatctt accagtcaag tatcatcgaa ccggcttgat aaccacacct
     2881 cgtgtaatag ctgagcagat agttgtcatt ttaaagcgtt ggcctttgtc gattatgtaa
     2941 tgcgcacatt caacacatgg taatatagaa acggttatgt cgaggttgtt ttgtccagag
     3001 atgaccttca cacagttaca gccgctctgc atccacacaa atggaggact taatcgtgga
     3061 ctgcattctt agaaatgatc tacaaagaca aataatgtga aatcaagaaa ggacaaaatt
     3121 taagtaaggg gatgagggag agagagaacg aggggcaagg agaaagcatg gctcctgtct
     3181 ttttctgcac ccatctgttc ggagtgcagg tggagctcta ttcactcagc tctgcatgtg
     3241 tgtttggggg gggcaggaag aaagggaggg caaaaggaag agtggagaga tggtgggggc
     3301 tggagggatg gggggttctc ggtgatctct cctgaagggg ataatgggag agcagcgctt
     3361 tgcaatggct gccatgtagt accctccctg cacaattagc caatcagcag caagctctgc
     3421 cagccagaag gacacataaa agaagaacat tgcagcagag gcacagaagg agcctgcgag
     3481 gagctgggaa atacacacac aacagcagaa ccacaacacc ctcccctgga cacaccctac
     3541 tggggatcac tgcttttctt tttttctgaa ccatcgccca cgccacacgg agagaaatct
     3601 ctctctcatc atcatcctga agaaaacccc cttatcctca ttttcacact gctgaggaaa
     3661 acctacaatc gcacgggctg agatttcctg gcgaagactg tcctttttcc tttttctttt
     3721 tttttctttt cctttggaaa ctgacatttg catttctcca ttccaagcca cggcgtaata
     3781 atatctgcaa tccagcctga agacctgcaa atcgaaggac ctagatcact atcatctttg
     3841 tacgtcaagg atcctggtta ctgtacgtat aacctttctt tcttttgctc tgaccaatat
     3901 gaaacactaa aatcgattcg agcagcctct cagcatcaat tacagtgcgt gaaaaacatt
     3961 caaatagagg cagcaaatat tacatgtgaa aatacaggct ggctaaatcc aagctaattt
     4021 agaaatgtgg tcaaaacgca tactggcacg tctaatcgcg gattcagtaa acaagattaa
     4081 cgattagccc agtgtataag tcatatgata ccggcatgcg cgagagcatc gctacacccg
     4141 agctggcttc attttcggag gaaaatcaaa acattgcttt ctcctgccgt gcgaaccatt
     4201 cgtcataaac cgtaatacgc aacatacatt tattactaca tccgttaatt agcgataatt
     4261 agccgttatt aacaaagagc gctgaggaat tcctctcaaa tagcggaggt gcggcggagg
     4321 cagaggggcg taaaagggca catgcgtgcc tggctcaaaa aaggatgctc cagactgaaa
     4381 ctcagagaca aaacacgatg cctcggaggc tgagagccca tcgagaggaa aggcaaagaa
     4441 aagggtcgag tcactcagag ggggagggga gatattgtgc atccattggt ttgaattgaa
     4501 gcaggcagaa taaaaatcct tgcgattatg cttcgtgaga gatcgcgaga gaggaaaagg
     4561 ctaaatgcca cgtatgcaaa tggataaatg tcatctattt cttcgcgagt agacattttg
     4621 tggacaacga gagtgtaaat aagcgatatg aggggaatac aaaggccttg agggaaagcg
     4681 ttttgggcgt gtcttgtcga aaagaagaca gcgatggcac gcgctcagaa ccaccatctt
     4741 cacgtagaca atccgcgtga aaacaataac aaaactgctc attttaacac gagcaggtgt
     4801 ttgaaaccac ccaaaaaaga ttgtctaaaa tattaagatt aattaatgac gtttaaatgt
     4861 ccaggcctga tataaatgaa gcgtgtagta agagtaatcg cctttgtttc ttttcagaga
     4921 tacgttttca gagattcagg gaattatatc ttcaataaaa taatgtttga tttcattatt
     4981 ttaaagcttt ttaagaagta aaaatacaca ttattcattt acattttatt ttatttaaag
     5041 gcctatggtc ttgaaaataa taagtggaat atataggcta tatattcgtc taaggacagc
     5101 cggtgttatt ttatagtgac ctcagacaaa agccgagaca aaacgaggcg cttgcctttc
     5161 attttaatcc ttaatagacg gtgtgatgaa tgaatgaatg aatgaataaa tgaatgaatg
     5221 acgggccctt cggcatgttg ttccgcagtc ctccgtgagc acgagtctct accgcagtgc
     5281 caagcacata aaaccgttga gtcagataac cttctccagg cgcgtataca caattcaagg
     5341 acaagcacat cacaactata cacaacacaa cacaaccgcc taaacgcacg agctcatcaa
     5401 tcgcaattta aaggcatgtc agtcaaagaa aaaggcggcg ttatttaaaa ccaatgttca
     5461 acaatatttg tatgctgact agcccgggcg cgcacgctat aagatcacaa ggccttgttt
     5521 tttcccttta ttcaaaatat cattaattat tacaggtcat aaaacaaaac aatgcgaatt
     5581 acaatgattt aaagactatt gatttaacaa taatcagtta attatagaat aatattataa
     5641 tattttacat acaaatgaaa gatgacatta tttgatgaca ttgtaattaa aatatatgca
     5701 atatattttt acgtttttat taattaggct attactatta ttataattat cattattttt
     5761 attattatta ttattattat taaatagtct tgcgaaataa gagtgaaggc agttgcaatg
     5821 ttcattgttc ccggagagtc ccgcagcctc tggcctcaac acaaagcgct actgtttaca
     5881 ttaatattca tagtgctgcc atggtctctg tagaggagga acgggtggct cttttgttcg
     5941 ctagtgtcaa tacgtttatt cagcaaaaag cagctcggat ttgtgaatgc ccaacaagca
     6001 cacgcgcact ccaatcaaag gacgagagga taacgatttt aaacgattcg caaacgtgtt
     6061 tatttgtaaa gacgacatca agagcttaat gtccacttga aaagaaataa atatgcccgc
     6121 ggcgccaagg atattttgaa tgtagtcgtt catggtgagc cactcccgaa ttcagcactc
     6181 tggagagcga ccaaagagga atagcggaat agcaactact tgagccattt ctctccgttt
     6241 gatggcgttc atgattaaaa atataatcac gtgatttttt atacatatat aaatatatac
     6301 ataaagtaac gctttccttt tgagaataca taaatattta ttcgcgtgat aaatcaatga
     6361 tcttatattt atttgcacga ccgaaattag ccaaattcac caattaaaaa aaaaataagc
     6421 caacaaaaaa gaagggttta tccgttcaat tttgacttgt gtcctgttgt tttacagctg
     6481 ccactgtgat cccttaagct gcagtgagag tgtggctaat gccttttgtt taagataatc
     6541 ctgtaatctg ttaccgaaac ggcctattga caagccggca ttcacatttc agtcaggagg
     6601 cccgtccaga catgtacatt tatgaattac gaatgataaa attaagatct gcattaggcg
     6661 tcagaaatgt cacgaacacc cattcatttc ccacagccaa taatggatca tgctgggaag
     6721 cgctatcctc gcagtctcaa aattaatgtt gacatgttgc gtagagctac agattagatc
     6781 agccatatgc tttatgtgtt tacctcagcg gatcttcagc aagatgtgtt tattttaaga
     6841 aaatggcttc ctcgctgcct gcacaggggc attaaggaag tgacgtgagc gtcctcacag
     6901 tcaaattact ttatctcatg ctgctttcag gcctctgctt attttattat tattatttta
     6961 aattaggggg aatcacggtg gcgttgtggg tagggtagcg cgatcacctt acagcaagaa
     7021 ggtcgctggt tcgagctctg gctgggtcag ttggcatttc tgtgtggagt ttaaattgaa
     7081 ttgaaattga ataaactaaa ttggccctag tgtatgtgtg tgaatgtaag tgtgcatggg
     7141 tgtttcccag tgttgggttt gactggaaga gcatccgctt cgtaaaacat atgctggata
     7201 agttggcggt tcatttcact gtggtgaccg ctgattaata aagggactaa gctgaaaatg
     7261 aaatgaatta atacatttta aatgagcttt tgctgacatg tttatgatta caaatagtga
     7321 aattgtatgt taatttacga taagtgcatt gttctaaatt catttttatg tagcagctgg
     7381 tttttcttcc atttgtgtca ttcaacagaa catttttcta aaaaagtgaa cttaaatttt
     7441 ctcctccctc tgacaaggct tgatggtaat ttcagccgac tctaattaca gactcactaa
     7501 aaggaatcgg tgcagtcatt tatctttatc agcgcgctgg tgggaaggta ataggttttg
     7561 cttatgagtt gtttgcgtgc gagtctgggt ccttgtgtag gcgatgctga tgcactgcat
     7621 taaatattga gggagaagtc gctcaaaaat agcaaagtac aggctggggc aggaacgtca
     7681 aagcccgctc agtggccacg tttcttgttt cgtaagtcct atttttaata gcgataattc
     7741 aggctcagta gaaggaactc cagcactata agcagtctct cgcctgtctc ggcgagttat
     7801 gtattttggg atgaagtgct cttccacctc tacctctgct gtgctggcgc cagtatctgg
     7861 gctgtaagat agtgtacgtg tcagtctgtg tggccgaaga gcctgtgtgt tgtgtaactc
     7921 actacattcg tcgctgtgga tggctgactt gccatgggga ggtttacttc agttcacgaa
     7981 tcacattcgc cacgatcttg tcactttgtg tcaagactcg ctctctgtgt gtcaacagct
     8041 ctgctgtttg aggttgaaat tctgaacttg agatatatcc caggatatat tagggctact
     8101 gccaacggaa aaagcaaatg gtgccatggc catttgattg atcaaacaaa cacccttttg
     8161 cttgggtgac agtgaaatgt cagatccatg tttgtactgc tttaataact tgcctctctg
     8221 agctttctcc ttgtcagtgt caatttgcct gagatataaa ttccggttgc cccgaaagct
     8281 tattttatta aaaaaaagct aacatgcact catacaacgt gcgcacatac atgtgaggct
     8341 tgctaaaagt caagaagcaa gccaatagca agagtcgtca gactgctgtt acctgggcaa
     8401 cctggttaga ggtaaggctc aaggcaagaa taaatcattc cactgaagag acacagcgtt
     8461 acagcgagat gagagcaaga ggcgatagtg aagggagaac aaaagaacag cctacccaca
     8521 ctagtgcaga tctacctgtt tgagctactg attgttcagt ttgattagat atcagacaat
     8581 tctgctatct ccaattgcat tgctgcatga attcctagaa atcattttaa tcaccaaaaa
     8641 ctagtaggga cacttgtggc gtatatgatt ctgcctgttg attgtgggga tccaacagaa
     8701 agatcatgaa tgcattgtta attaagtcgg tgagacaccc tgttccaccc taggggccgt
     8761 tctggggaaa gagtgctttc agtcagctaa agatgactaa tatgtgaaca tatattacta
     8821 cacatgccaa tttgtacatt ctggatagtt accagccctg ggaaaacatc gaaaaaaata
     8881 aatcaacata gataaaattg gcaaatccct tctggtacga tgcagagtgc ttgctctgag
     8941 aacttgtgca ttgtgacaga aagcgaccgt taaaaactga aagtagcatc tacaacctgc
     9001 atacatctgt tctttgaaaa tcccctgtag gccaaattaa gcatcacgct gtgctccaca
     9061 gcaacacgga tgaaccgaaa acccagacac aaacgcacac acttttcaca taaacaaggt
     9121 ttagttatgc tagctatgct ttttcgtcct ccctttgtca tcagcggcga gtgacgtaac
     9181 acagtttgac cctggacagc gtaaaggggg ggttgggaat gagtgaaagg actgtgacat
     9241 ctgcagggcc aggggctgat ggagagagct gaagtgaggg aggtcagagg aggaggaagg
     9301 gtgggatatt gtcctcctca ggccctatcc ctctcagacg cattgatttt taacctctca
     9361 gcgagaatgc caagcattac acccctcaaa ttatggttcc tcacccaacc tgttatattt
     9421 tccacctgca gataattacc ggtatgctgc agaacgagct tgctcttaag ttggctggac
     9481 ttgatattaa caagactgga ggttctcatc atcaccacca ccatggatcc gaccaactga
     9541 ctgaagagca gatcgcagaa tttaaagagg ctttctccct atttgacaag gacggggatg
     9601 ggacaataac aaccaaggag ctggggacgg tgatgcggtc tctggggcag aaccccacag
     9661 aagcagagct gcaggacatg atcaatgaag tagatgccga cggtgacggc acaatcgact
     9721 tccctgagtt cctgacaatg atggcaagaa aaatgaaaga cacagacagt gaagaagaaa
     9781 ttagagaagc gttccgtgtg tttgataagg atggcaatgg ctacatcagt gcagcagagc
     9841 ttcgccacgt gatgacaaac cttggagaga agttaacaga tgaagaggtt gatgaaatga
     9901 tcagggaagc agacatcgat ggggatggtc aggtaaacta cgaagagttt gtagtgatga
     9961 tgacagcgaa gctcgagtgc gagaaaatct atgtgcgtga tggagtgctg acgggtgata
    10021 ttcatatgat cttgttgctt gaaggaaatg cccattaccg atgtgacttc agaactactt
    10081 acaaagctaa ggagaagggt gtcaagttac caggcgtgca ctatgtggac cacaccattg
    10141 agattttaag ccatgacaaa gattacaaca aggttaagat ctatgagtat gctgttgctc
    10201 attctggatt gcctgacaat gccagacgag gcggtaccgg cggatccatg gtgagtgcga
    10261 ttaagccaga catgaagatc aaactgcgta tggaaggcaa cgtaaacggg caccactttg
    10321 tgatcgacgg agatggtaca ggcaagcctt atgagggaaa acagaccatg gatcttgaag
    10381 tcaaagaggg cggacctctg ccttttgcct ttgatatcct gaccactgca ttccattacg
    10441 gcaacagggt attcgtgaaa tatccagaca acatacaaga ctattttaag cagtcgtttc
    10501 ctaaggggta ttcgtgggaa cgaagcatga ctttcgaaga cgggggcatt tgctatgcca
    10561 gaagcgacat aacaatggaa ggggacactt tctataataa agttcgattt tatggtacca
    10621 actttcccgc caatggtcca gttatgcaga agaagacgct gaaatggatg ccgagctgga
    10681 cgcgttcatc acgtcgtaag ttcaataaga caggtcacgc actgagagct ataggtcggc
    10741 tgagctcagg aggttctgga ggttctgatt acaaggacga cgatgacaag ggaggttctg
    10801 gaggttctta cccatacgac gtaccagatt acgctggagg ttctggaggt tctgagcaga
    10861 agctgatctc agaggaggac ctgcgtacgt aaccggtgga tcctggccgt cgttttactg
    10921 taccatcgat gatgatccag acatgataag atacattgat gagtttggac aaaccacaac
    10981 tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt
    11041 aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca
    11101 ggttcagggg gaggtgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtat
    11161 ggctgattat gatcctctag atcagatctg cgaagatacg gccacgggtg ctcttgatcc
    11221 tgtggctgat tttggactgt gctgctcgca gctgctgatg aatcacatac ttcctccatt
    11281 ttcttccact gattgactgt tataatttcc ctaatttcca ggtcaaggtg ctgtgcattg
    11341 tggtaataga tgtgacatga cgtcacttcc aaaggaccaa tgaacatgtc tgaccaattt
    11401 catataatgt gaaaacgatt ttcataggca gaataaataa catttaaatt aaactgggca
    11461 tcagcgcaat tcaattggtt tggtaatagc aagggaaaat agaatgaagt gatctccaaa
    11521 aaataagtac tttttgactg taaataaaat tgtaaggagt aaaaagtact tttttttcta
    11581 aaaaaatgta attaagtaaa agtaaaagta ttgattttta attgtactca agtaaagtaa
    11641 aaatccccaa aaataatact taagtacagt aatcaagtaa aattactcaa gtactttaca
    11701 cctctggttc ttgaccccct accttcagca agcccagcag atccactagt tctagagcgg
    11761 ccgccaccgc ggtggagctc cagcttttgt tccctttagt gagggttaat tgcgcgcttg
    11821 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
    11881 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
    11941 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
    12001 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
    12061 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
    12121 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
    12181 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
    12241 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
    12301 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
    12361 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
    12421 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
    12481 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
    12541 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
    12601 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
    12661 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
    12721 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
    12781 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
    12841 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aaggcatttt ggtcatgaga
    12901 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
    12961 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
    13021 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
    13081 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
    13141 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
    13201 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
    13261 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
    13321 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
    13381 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
    13441 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
    13501 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
    13561 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
    13621 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
    13681 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
    13741 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
    13801 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
    13861 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
    13921 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
    13981 cctaaattgt aagcgt
//
