LOCUS       sequence-27439-e         699 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 699)
  AUTHORS   Lee HH, Elia N, Ghirlando R, Lippincott-Schwartz J, Hurley JH
  TITLE     Midbody targeting of the ESCRT machinery by a noncanonical coiled 
            coil in CEP55.
  JOURNAL   Science. 2008 Oct 24. 322(5901):576-80.
  PUBMED    18948538
REFERENCE   2  (bases 1 to 699)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2008 Oct 24. 322(5901):576-80."
COMMENT     Sequence Label: pGST2-ALIX (795-811)
FEATURES             Location/Qualifiers
     source          1..699
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             15..35
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     primer_bind     complement(167..189)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(334..352)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        420..524
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aaatcgatac gaccgaaaac ctgtattttc agggcgccat gggatcccca ccacaggccc
       61 aaggcccacc atacccaacg tacccaggct atccaggctg actcgagcgg ccgcatcgtg
      121 actgactgac gatctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc
      181 agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc
      241 agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc catgacccag tcacgtagcg
      301 atagcggagt gtataattct tgaagacgaa agggcctcgt gatacgccta tttttatagg
      361 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc
      421 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
      481 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
      541 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
      601 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
      661 aactggatct caacaggcgg taagatcctt gagagtttt
//
