LOCUS       sequence-274392-        5038 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  full length ced-4L cDNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5038)
  AUTHORS   Shaham S, Horvitz HR
  TITLE     An alternatively spliced C. elegans ced-4 RNA encodes a novel cell 
            death inhibitor.
  JOURNAL   Cell. 1996 Jul 26;86(2):201-8. doi: 10.1016/s0092-8674(00)80092-6.
  PUBMED    8706125
REFERENCE   2  (bases 1 to 5038)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Cell"; date: "1996-07-26- 26"; volume: "86"; issue: "2"; pages: 
            "201-8"
COMMENT     Sequence Label: pS241
FEATURES             Location/Qualifiers
     source          1..5038
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     127..144
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    261..282
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(297..302,303..320,321..327)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    335..351
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     340..362
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     359..375
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     359..375
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2321..2338)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2321..2337)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2330..2352)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      2550..3005
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2637..2656)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2847..2868
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3022..3041)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3141..3163
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3201..3219)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3287..3391
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3392..3460,3461..4252)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3610..3629)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4423..5011
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4912..4931
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
       61 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
      121 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
      181 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg
      241 ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta
      301 cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca
      361 ggaaacagct atgaccatga ttacgccaag cttaaggtgc acggcccacg tggccactag
      421 tatgctctgc gaaatcgaat gccgcgcttt gagcacggca cacacgaggc tcatccacga
      481 ctttgaacca cgtgacgcat tgacttattt agaaggcaaa aacattttca cagaagatca
      541 ttctgaactt atcagtaaaa tgtcaactcg cctcgagagg atcgccaatt ttcttcgaat
      601 ctatcgacgt caagcttctg aacttggacc actcatcgac tttttcaact acaacaatca
      661 aagtcacctt gctgatttcc tcgaagacta catcgatttt gcgataaatg agccagatct
      721 acttcgtcca gtagtgattg ctccacaatt ttcccgacaa atgctcgata ggaaactatt
      781 gcttgggaat gttccaaaac aaatgacatg ctatattcga gagtatcacg tggatcgagt
      841 gatcaaaaag ctcgacgaga tgtgtgattt agactccttt tttctgtttc tacacggccg
      901 agctggatcc ggaaaatcag taattgcatc acaagctctt tcgaaatctg accaacttat
      961 tggaataaat tatgattcaa tcgtttggct caaagatagt ggaacagctc caaaatctac
     1021 attcgattta tttacggata ttttgctgat gctaaaacga gcccgtgttg tgagcgacac
     1081 ggatgactcg cattcgatca ccgacttcat taaccgtgtt ctttcaagaa gcgaagacga
     1141 tcttctcaat ttcccatcgg tggagcatgt cacgtcagtt gtactcaaaa ggatgatctg
     1201 caacgcactc attgatcgtc caaatacttt attcgtattt gatgacgtag ttcaagaaga
     1261 aacaattcgt tgggctcagg agctacgtct tcgatgtctt gtaactactc gtgacgtgga
     1321 aatatcaaat gctgcttctc aaacatgcga attcattgaa gtgacatcat tggaaatcga
     1381 tgaatgttat gattttctag aagcttatgg aatgccgatg cctgttggag aaaaagaaga
     1441 agatgtgctt aataaaacaa tcgaactaag cagtggaaat ccagcaacgc ttatgatgtt
     1501 tttcaagtct tgtgaaccga aaacatttga aaaaatggca cagcttaata acaaattgga
     1561 aagtcgagga ttagtcggtg ttgaatgtat caccccttac tcgtacaagt cactcgcaat
     1621 ggctcttcaa agatgtgttg aagttttgtc agatgaggat cgaagtgctc ttgctttcgc
     1681 agttgtgatg cctcctggag ttgatatacc cgtcaagcta tggtcatgtg ttattccagt
     1741 tgatatttgt tcaaatgaag aagaacaatt ggatgatgaa gttgcggatc ggttgaaaag
     1801 actcagcaaa cgtggagctc ttctcagtgg aaaacgaatg cccgttttga cattcaaaat
     1861 tgatcatatt atccatatgt tcttgaaaca cgtcgttgat gcacaaacta tcgccaatgg
     1921 aatctcaatt ctcgagcagc gtcttcttga aataggaaac aataatgtat cagtaccgga
     1981 gcgacatata ccatcacatt tccaaaaatt ccgtcgttca tcagccagtg agatgtatcc
     2041 aaaaactaca gaagaaactg tgatccgtcc tgaagacttc ccaaagttca tgcaattgca
     2101 ccagaaattc tatgactccc tcaaaaattt tgcatgctgt taaaacctat cgtgtacaat
     2161 attgcctgta tattcccctc gaaatacgtt tatacttttt cgcacgagtt ttctcatttt
     2221 ttcatttgta cttgttttat ttctctccaa aatttcagat ccatatatag ggcccgggtt
     2281 ataattacct caggtcgacg tcccatggcc attcgaattc actggccgtc gttttacaac
     2341 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt
     2401 tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca
     2461 gcctgaatgg cgaatggcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt
     2521 cacaccgcat acgtcaaagc aaccatagta cgcgccctgt agcggcgcat taagcgcggc
     2581 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccttag cgcccgctcc
     2641 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     2701 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     2761 tgatttgggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     2821 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     2881 ctctatctcg ggctattctt ttgatttata agggattttg ccgatttcgg tctattggtt
     2941 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgtttac
     3001 aattttatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg
     3061 acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta
     3121 cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc
     3181 gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat
     3241 aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
     3301 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     3361 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     3421 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     3481 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     3541 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     3601 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
     3661 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     3721 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     3781 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     3841 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     3901 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
     3961 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     4021 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     4081 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
     4141 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     4201 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     4261 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     4321 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     4381 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     4441 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     4501 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     4561 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     4621 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     4681 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     4741 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     4801 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     4861 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     4921 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     4981 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacgg
//
