LOCUS       sequence-274398-        9601 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for co-expressing Cre recombinase and sgRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9601)
  AUTHORS   Olivero CE, Martinez-Terroba E, Zimmer J, Liao C, Tesfaye E, 
            Hooshdaran N, Schofield JA, Bendor J, Fang D, Simon MD, Zamudio JR, 
            Dimitrova N
  TITLE     p53 Activates the Long Noncoding RNA Pvt1b to Inhibit Myc and 
            Suppress Tumorigenesis.
  JOURNAL   Mol Cell. 2020 Feb 20;77(4):761-774.e8. doi: 
            10.1016/j.molcel.2019.12.014. Epub 2020 Jan 20.
  PUBMED    31973890
REFERENCE   2  (bases 1 to 9601)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2019.12.014"; journalName: "Mol Cell"; date: 
            "2020-02-20- 20"; volume: "77"; issue: "4"; pages: "761-774.e8"
COMMENT     Sequence Label: UGPC
FEATURES             Location/Qualifiers
     source          1..9601
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     36..55
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             113..133
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             134..1162
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="SNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKMLL
                     SVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGLP
                     RPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAFL
                     GIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVER
                     WISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQR
                     YLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLED
                     GD"
     misc_feature    1215..1803
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1268..1288)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1686..1697)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(1806..1822)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1807..1823)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             2332..2512
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(2574..3162)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2654..2673)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3333..4124,4125..4193))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3956..3975
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4194..4298)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(4373..4392)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        4564..4943
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4945..5143
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5098..5118
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             5161..5341
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    5388..5513
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6010..6243
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6428..6472
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        6779..7027
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     6779..6799
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     6950..6969
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        8909..8984
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    9032..9149
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        join(9205..9601,1..103)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(9240..9263)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
ORIGIN
        1 cggggcgggc gcccgaaggt cctccggagg cccggcattc tgcacgcttc aaaagcgcac
       61 gtctgccgcg ctgttctcct cttcctcatc tccgggaccc ggggccacca tgcccaagaa
      121 gaagaggaag gtgtccaatt tactgaccgt acaccaaaat ttgcctgcat taccggtcga
      181 tgcaacgagt gatgaggttc gcaagaacct gatggacatg ttcagggatc gccaggcgtt
      241 ttctgagcat acctggaaaa tgcttctgtc cgtttgccgg tcgtgggcgg catggtgcaa
      301 gttgaataac cggaaatggt ttcccgcaga acctgaagat gttcgcgatt atcttctata
      361 tcttcaggcg cgcggtctgg cagtaaaaac tatccagcaa catttgggcc agctaaacat
      421 gcttcatcgt cggtccgggc tgccacgacc aagtgacagc aatgctgttt cactggttat
      481 gcggcggatc cgaaaagaaa acgttgatgc cggtgaacgt gcaaaacagg ctctagcgtt
      541 cgaacgcact gatttcgacc aggttcgttc actcatggaa aatagcgatc gctgccagga
      601 tatacgtaat ctggcatttc tggggattgc ttataacacc ctgttacgta tagccgaaat
      661 tgccaggatc agggttaaag atatctcacg tactgacggt gggagaatgt taatccatat
      721 tggcagaacg aaaacgctgg ttagcaccgc aggtgtagag aaggcactta gcctgggggt
      781 aactaaactg gtcgagcgat ggatttccgt atctggtgta gctgatgatc cgaataacta
      841 cctgttttgc cgggtcagaa aaaatggtgt tgccgcgcca tctgccacca gccagctatc
      901 aactcgcgcc ctggaaggga tttttgaagc aactcatcga ttgatttacg gcgctaagga
      961 tgactctggt cagagatacc tggcctggtc tggacacagt gcccgtgtcg gagccgcgcg
     1021 agatatggcc cgcgctggag tttcaatacc ggagatcatg caagctggtg gctggaccaa
     1081 tgtaaatatt gtcatgaact atatccgtaa cctggatagt gaaacagggg caatggtgcg
     1141 cctgctggaa gatggcgatt agaaacgttg ttgtttgggg ttgaattact cttctagacc
     1201 gcggcgcgcc cgataatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
     1261 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
     1321 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc
     1381 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg
     1441 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg
     1501 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
     1561 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct
     1621 ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg
     1681 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc
     1741 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc
     1801 cgcatcgata ccgtcgacct cgatcgagac ctagaaaaac atggagcaat cacaagtagc
     1861 aatacagcag ctaccaatgc tgattgtgcc tggctagaag cacaagagga ggaggaggtg
     1921 ggttttccag tcacacctca ggtaccttta agaccaatga cttacaaggc agctgtagat
     1981 cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga
     2041 caagatatcc ttgatctgtg gatctaccac acacaaggct acttccctga ttggcagaac
     2101 tacacaccag ggccagggat cagatatcca ctgacctttg gatggtgcta caagctagta
     2161 ccagttgagc aagagaaggt agaagaagcc aatgaaggag agaacacccg cttgttacac
     2221 cctgtgagcc tgcatgggat ggatgacccg gagagagaag tattagagtg gaggtttgac
     2281 agccgcctag catttcatca catggcccga gagctgcatc cggactgtac tgggtctctc
     2341 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     2401 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     2461 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagcatgtga
     2521 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     2581 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     2641 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     2701 gttccgaccc tgccgcttat cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     2761 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     2821 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     2881 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     2941 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     3001 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     3061 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     3121 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     3181 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     3241 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     3301 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     3361 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     3421 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     3481 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     3541 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     3601 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     3661 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     3721 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     3781 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     3841 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     3901 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     3961 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     4021 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     4081 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     4141 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     4201 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     4261 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     4321 cctgacgtcg acggatcggg agatctcccg atcccctatg gtgcactctc agtacaatct
     4381 gctctgatgc cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg
     4441 agtagtgcgc gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattgcatga
     4501 agaatctgct tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc
     4561 gttgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
     4621 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
     4681 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
     4741 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
     4801 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
     4861 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
     4921 tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat
     4981 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt
     5041 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc
     5101 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagcgcgttt tgcctgtact
     5161 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
     5221 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
     5281 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     5341 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca
     5401 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc
     5461 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta
     5521 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa
     5581 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc
     5641 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc
     5701 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg
     5761 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc
     5821 aaaacaaaag taagaccacc gcacagcaag cggccggccg ctgatcttca gacctggagg
     5881 aggagatatg agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga
     5941 accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc
     6001 agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc
     6061 agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca
     6121 gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg
     6181 catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct
     6241 cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc
     6301 tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga
     6361 cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca
     6421 gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg
     6481 gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt
     6541 ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata
     6601 ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg
     6661 aatagaagaa gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc
     6721 ggcactgcgt gcgccaattc tgcagatatg aaataggccc tctctggctg cagccagaga
     6781 gggcctattt cccatgattc cttcatattt gcatatacga tacaaggctg ttagagagat
     6841 aattagaatt aatttgactg taaacacaaa gatattagta caaaatacgt gacgtagaaa
     6901 gtaataattt cttgggtagt ttgcagtttt aaaattatgt tttaaaatgg actatcatat
     6961 gcttaccgta acttgaaagt atttcgattt cttggcttta tatatcttgt ggaaaggacg
     7021 aaacaccgga gacggttgta aatgagcaca caaaatacac atgctaaaat attatattct
     7081 atgaccttta taaaatcaac caaaatcttc tttttaataa ctttagtatc aataattaga
     7141 atttttatgt tcctttttgc aaacttttaa taaaaatgag caaaataaaa aaacgctagt
     7201 tttagtaact cgcgttgttt tcttcacctt taataatagc tactccacca cttgttccta
     7261 agcggtcagc tcctgcttca atcatttttt gagcatcttc aaatgttcta actccaccag
     7321 ctgctttaac taaagcattg tctttaacaa ctgacttcat tagtttaaca tcttcaaatg
     7381 ttgcacctga ttttgaaaat cctgttgatg ttttaacaaa ttctaatcca gcttcaacag
     7441 ctatttcaca agctttcatg atttcttctt ttgttaataa acaattttcc ataatacatt
     7501 taacaacatg tgatccagct gcttttttta cagctttcat gtcttctaaa actaattcat
     7561 aatttttgtc ttttaatgca ccaatattta ataccatatc aatttctgtt gcaccatctt
     7621 taattgcttc agaaacttcg aatgcttttg tagctgttgt gcatgcacct agaggaaaac
     7681 ctacaacatt tgttattcct acatttgtgc cttttaataa ttctttacaa tagcttgttc
     7741 aatatgaatt aacacaaact gttgcaaaat caaattcaat tgcttcatca cataattgtt
     7801 taatttcagc tttcgtagca tcttgtttta ataatgtgtg atctatatat ttgtttagtt
     7861 tcattttttc tcctatatat tcatttttaa ttttaattct ttaataattt cgtctacttt
     7921 aactttagcg ttttgaacag attcaccaac acctataaaa taaattttta gtttaggttc
     7981 agttccactt gggcgaacag caaatcatga cttatcttct aaataaaatt ttagtaagtc
     8041 ttgtcctggc atattataca ttccatcgat gtagtcttca acattaacaa ctttaagtcc
     8101 agcaatttga gttaagggtg ttgctctcaa tgatttcatt aatggttcaa tttttaattt
     8161 cttttcttct ggtttaaaat tcaagtttaa agtgaaagtg taatatgcac ccatttcttt
     8221 aaataaatct tctaaatagt ctactaatgt tttattttgt tttttataaa atcaagcagc
     8281 ctctgctatt aatatagaag cttgtattcc atctttatct ctagctgagt catcaattac
     8341 atatccataa ctttcttcat aagcaaaaac aaaatttaat ccgttatctt cttctttagc
     8401 aatttctcta cccattcatt taaatccagt taaagttttt acaatattaa ctccatattt
     8461 ttcatgagcg attctatcac ccaaatcact tgttacaaaa cttgaatata gagccggatt
     8521 ttttggaatg ctatttaagc gttttagatt tgataatttt caatcaatta aaattggtcc
     8581 tgtttgattt ccatctaatc ttacaaaatg accatcatgt tttattgcca ttccaaatct
     8641 gtcagcatct gggtcattca taataataat atctgcatca tgtttaatac catattcaag
     8701 cggtattttt catgcaggat caaattctgg atttggattt acaacatttt taaatgtttc
     8761 atcttcaaat gcatgctctt caacctcaat aacgttatat cctgattcac gtaatatttt
     8821 tggggtaaat ttagttcctg ttccattaac tgcgctaaaa ataattttta aatctttttt
     8881 agcttcttgc tcttttttgt acgtctctgt tttagagcta gaaatagcaa gttaaaataa
     8941 ggctagtccg ttatcaactt gaaaaagtgg caccgagtcg gtgctttttt gaattctaga
     9001 tcttgagaca aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt
     9061 acagtgcagg ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac
     9121 aaaaacaaat tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccac
     9181 tttggcgcgc ggccgcattc taccgggtag gggaggcgct tttcccaagg cagtctggag
     9241 catgcgcttt agcagccccg ctgggcactt ggcgctacac aagtggcctc tggcctcgca
     9301 cacattccac atccaccggt aggcgccaac cggctccgtt ctttggtggc cccttcgcgc
     9361 caccttctac tcctccccta gtcaggaagt tcccccccgc cccgcagctc gcgtcgtgca
     9421 ggacgtgaca aatggaagta gcacgtatca ctagtctcgt gcagatggac agcaccgctg
     9481 agcaatggaa gcgggtaggc ctttggggca gcggccaata gcagctttgc tccttcgctt
     9541 tctgggctca gaggctggga aggggtgggt ccgggggcgg gctcaggggc gggctcaggg
     9601 g
//
