LOCUS       sequence-274400-        9315 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral inducible expression of mCherry with nuclear localization
            signal.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9315)
  AUTHORS   Taylor-Weiner H, Grigsby CL, Ferreira DMS, Dias JM, Stevens MM, Ruas
            JL, Teixeira AI
  TITLE     Modeling the transport of nuclear proteins along single skeletal 
            muscle cells.
  JOURNAL   Proc Natl Acad Sci U S A. 2020 Feb 11;117(6):2978-2986. doi: 
            10.1073/pnas.1919600117. Epub 2020 Jan 27.
  PUBMED    31988126
REFERENCE   2  (bases 1 to 9315)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1919600117"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2020-02-11- 11"; volume: "117"; issue: "6"; pages: 
            "2978-2986"
COMMENT     Sequence Label: pFUW-TetO-mCherry-cNLS
FEATURES             Location/Qualifiers
     source          1..9315
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(69..87)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     337..356
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             656..676
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             680..700
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             704..724
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    868..1456
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(921..941)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1339..1350)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(1459..1475)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1460..1476)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             1981..2161
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(2187..2204)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2193..2417
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      2463..2891
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2550..2569)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2760..2781
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(2900..2920)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2905..3234
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3085..3220
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     3147..3166
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        3282..3329
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             3348..3722
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    3852..3973
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3889..3908)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3943..3962
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4022..4038)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4022..4038)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4035..4057)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4046..4062
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4070..4076,4077..4094,4095..4100))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4115..4136
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4253..4270)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4424..5012)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4504..4523)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5183..5974,5975..6043))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5806..5825
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6044..6148)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(6223..6242)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        6414..6793
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6795..6993
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             7011..7191
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7238..7363
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7856..8089
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8274..8318
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8616..8733
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(8786..8804,8805..8827,8828..8846,8847..8869,8870..8888,
                     8889..8911,8912..8930,8931..8953,8954..8972,8973..8995,
                     8996..9014,9015..9037,9038..9056)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        9089..9127
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9124..9148
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      9233..9242
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(9239..9315,1..631)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
ORIGIN
        1 cgtgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac
       61 ccagaccgcc aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct
      121 gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag caccccgccg acatccccga
      181 ctacttgaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga
      241 cggcggcgtg gtgaccgtga cccaggactc ctccctgcag gacggcgagt tcatctacaa
      301 ggtgaagctg cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat
      361 gggctgggag gcctcctccg agcggatgta ccccgaggac ggcgccctga agggcgagat
      421 caagcagagg ctgaagctga aggacggcgg ccactacgac gctgaggtca agaccaccta
      481 caaggccaag aagcccgtgc agctgcccgg cgcctacaac gtcaacatca agttggacat
      541 cacctcccac aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca
      601 ctccaccggc ggcatggacg agctgtacaa gtccggactc agatctcgag ctgatccaaa
      661 aaagaagaga aaggtagatc caaaaaagaa gagaaaggta gatccaaaaa agaagagaaa
      721 ggtaggatcc accggatcta gataactgat cagctagcta tcaggatcct acgaggcgcg
      781 ccagatagaa ttctcttggg cctcaagggc catgagttaa ccagttctag atgtgactca
      841 tgtcaattcg atatcaagct tatcgataat caacctctgg attacaaaat ttgtgaaaga
      901 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
      961 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
     1021 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
     1081 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
     1141 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
     1201 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
     1261 aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg
     1321 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
     1381 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt
     1441 tgggccgcct ccccgcatcg ataccgtcga cctcgagacc tagaaaaaca tggagcaatc
     1501 acaagtagca atacagcagc taccaatgct gattgtgcct ggctagaagc acaagaggag
     1561 gaggaggtgg gttttccagt cacacctcag gtacctttaa gaccaatgac ttacaaggca
     1621 gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc
     1681 caacgaagac aagatatcct tgatctgtgg atctaccaca cacaaggcta cttccctgat
     1741 tggcagaact acacaccagg gccagggatc agatatccac tgacctttgg atggtgctac
     1801 aagctagtac cagttgagca agagaaggta gaagaagcca atgaaggaga gaacacccgc
     1861 ttgttacacc ctgtgagcct gcatgggatg gatgacccgg agagagaagt attagagtgg
     1921 aggtttgaca gccgcctagc atttcatcac atggcccgag agctgcatcc ggactgtact
     1981 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
     2041 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
     2101 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     2161 agggcccgtt taaacccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt
     2221 tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc
     2281 ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg
     2341 tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga
     2401 tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc
     2461 ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
     2521 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
     2581 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
     2641 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
     2701 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
     2761 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
     2821 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
     2881 taacgcgaat taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc
     2941 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag
     3001 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     3061 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
     3121 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct
     3181 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc
     3241 ccgggagctt gtatatccat tttcggatct gatcagcacg tgttgacaat taatcatcgg
     3301 catagtatat cggcatagta taatacgaca aggtgaggaa ctaaaccatg gccaagttga
     3361 ccagtgccgt tccggtgctc accgcgcgcg acgtcgccgg agcggtcgag ttctggaccg
     3421 accggctcgg gttctcccgg gacttcgtgg aggacgactt cgccggtgtg gtccgggacg
     3481 acgtgaccct gttcatcagc gcggtccagg accaggtggt gccggacaac accctggcct
     3541 gggtgtgggt gcgcggcctg gacgagctgt acgccgagtg gtcggaggtc gtgtccacga
     3601 acttccggga cgcctccggg ccggccatga ccgagatcgg cgagcagccg tgggggcggg
     3661 agttcgccct gcgcgacccg gccggcaact gcgtgcactt cgtggccgag gagcaggact
     3721 gacacgtgct acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa
     3781 tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct
     3841 tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     3901 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     3961 tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat
     4021 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     4081 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     4141 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     4201 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     4261 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     4321 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     4381 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     4441 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     4501 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     4561 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     4621 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     4681 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     4741 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     4801 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     4861 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     4921 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     4981 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     5041 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     5101 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     5161 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     5221 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     5281 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     5341 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     5401 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     5461 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     5521 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     5581 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     5641 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     5701 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     5761 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     5821 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     5881 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     5941 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     6001 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     6061 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     6121 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg
     6181 acggatcggg agatctcccg atcccctatg gtgcactctc agtacaatct gctctgatgc
     6241 cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc
     6301 gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattgcatga agaatctgct
     6361 tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc gttgacattg
     6421 attattgact agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat
     6481 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
     6541 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
     6601 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
     6661 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
     6721 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
     6781 cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga
     6841 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
     6901 aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg
     6961 taggcgtgta cggtgggagg tctatataag cagcgcgttt tgcctgtact gggtctctct
     7021 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     7081 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     7141 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc
     7201 gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct
     7261 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
     7321 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
     7381 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
     7441 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
     7501 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
     7561 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
     7621 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
     7681 taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga gatatgaggg
     7741 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     7801 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     7861 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc
     7921 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     7981 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     8041 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     8101 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     8161 aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca
     8221 attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg
     8281 aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa
     8341 attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa
     8401 tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt
     8461 ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag
     8521 gtggagagag agacagagac agatccattc gattagtgaa cggatcggca ctgcgtgcgc
     8581 caattctgca gacaaatggc agtattcatc cacaatttta aaagaaaagg ggggattggg
     8641 gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa
     8701 ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat
     8761 ccagtttggt tatcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt
     8821 ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gtttaccact ccctatcagt
     8881 gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga aaagtgaaag
     8941 tcgagtttac cactccctat cagtgataga gaaaagtgaa agtcgagttt accactccct
     9001 atcagtgata gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga tagagaaaag
     9061 tgaaagtcga gctcggtacc cgggtcgagg taggcgtgta cggtgggagg cctatataag
     9121 cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct
     9181 ccatagaaga caccgggacc gatccagcct ccgcggcccc gaattacagg ccgccaccat
     9241 ggtgagcaag ggcgaggagg ataacatggc catcatcaag gagttcatgc gcttcaaggt
     9301 gcacatggag ggctc
//
