LOCUS       sequence-274404-        9282 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral inducible expression of DsRed-Express2 with nuclear 
            localization signal.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9282)
  AUTHORS   Taylor-Weiner H, Grigsby CL, Ferreira DMS, Dias JM, Stevens MM, Ruas
            JL, Teixeira AI
  TITLE     Modeling the transport of nuclear proteins along single skeletal 
            muscle cells.
  JOURNAL   Proc Natl Acad Sci U S A. 2020 Feb 11;117(6):2978-2986. doi: 
            10.1073/pnas.1919600117. Epub 2020 Jan 27.
  PUBMED    31988126
REFERENCE   2  (bases 1 to 9282)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1919600117"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2020-02-11- 11"; volume: "117"; issue: "6"; pages: 
            "2978-2986"
COMMENT     Sequence Label: pFUW-TetO-DsRed-cNLS
FEATURES             Location/Qualifiers
     source          1..9282
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        27..406
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        408..606
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             624..804
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    851..976
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1469..1702
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1887..1931
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2229..2346
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(2399..2417,2418..2440,2441..2459,2460..2482,2483..2501,
                     2502..2524,2525..2543,2544..2566,2567..2585,2586..2608,
                     2609..2627,2628..2650,2651..2669)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2702..2740
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2737..2761
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2846..2855
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2852..3526
                     /codon_start=1
                     /product="noncytotoxic tetrameric variant of DsRed 
                     fluorescent protein (Strack et al., 2008)"
                     /label=DsRed-Express2
                     /note="mammalian codon-optimized"
                     /translation="MDSTENVIKPFMRFKVHMEGSVNGHEFEIEGEGEGKPYEGTQTAK
                     LQVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGTFIYHVKFIGVNFPSDGPVMQKKTLGWEPSTERLYPRDGVLKGEIHK
                     ALKLKGGGHYLVEFKSIYMAKKPVKLPGYYYVDSKLDITSHNEDYTVVEQYERAEARHH
                     LFQ"
     primer_bind     complement(3032..3052)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     3250..3269
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     3444..3467
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     CDS             3551..3571
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3575..3595
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3599..3619
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    3763..4351
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3816..3836)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4234..4245)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4354..4370)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4355..4371)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             4876..5056
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5082..5099)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5088..5312
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5358..5786
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5445..5464)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5655..5676
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5795..5815)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5800..6129
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5980..6115
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6042..6061
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6177..6224
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6243..6617
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    6747..6868
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6784..6803)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6838..6857
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6917..6933)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6917..6933)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6930..6952)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6941..6957
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6965..6971,6972..6989,6990..6995))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7010..7031
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7148..7165)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7319..7907)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7399..7418)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8078..8869,8870..8938))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8701..8720
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8939..9043)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9118..9137)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 cgatgtacgg gccagatata cgcgttgaca ttgattattg actagttatt aatagtaatc
       61 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
      121 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
      181 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
      241 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
      301 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
      361 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
      421 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
      481 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
      541 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
      601 aagcagcgcg ttttgcctgt actgggtctc tctggttaga ccagatctga gcctgggagc
      661 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
      721 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
      781 agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa
      841 accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga
      901 ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga
      961 tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg
     1021 gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga
     1081 gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat
     1141 actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa
     1201 tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc
     1261 tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgc
     1321 tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat
     1381 ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg
     1441 tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag
     1501 caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt
     1561 ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt
     1621 tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat
     1681 acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca
     1741 ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca
     1801 cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa
     1861 ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat
     1921 gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata aaattattca
     1981 taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga
     2041 atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg
     2101 gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca
     2161 ttcgattagt gaacggatcg gcactgcgtg cgccaattct gcagacaaat ggcagtattc
     2221 atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
     2281 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa
     2341 aattttcggg tttattacag ggacagcaga gatccagttt ggttatcgag tttaccactc
     2401 cctatcagtg atagagaaaa gtgaaagtcg agtttaccac tccctatcag tgatagagaa
     2461 aagtgaaagt cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta
     2521 ccactcccta tcagtgatag agaaaagtga aagtcgagtt taccactccc tatcagtgat
     2581 agagaaaagt gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg
     2641 agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagctcggt acccgggtcg
     2701 aggtaggcgt gtacggtggg aggcctatat aagcagagct cgtttagtga accgtcagat
     2761 cgcctggaga cgccatccac gctgttttga cctccataga agacaccggg accgatccag
     2821 cctccgcggc cccgaattac aggccgccac catggatagc actgagaacg tcatcaagcc
     2881 cttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt tcgagatcga
     2941 gggcgagggc gagggcaagc cctacgaggg cacccagacc gccaagctgc aggtgaccaa
     3001 gggcggcccc ctgcccttcg cctgggacat cctgtccccc cagttccagt acggctccaa
     3061 ggtgtacgtg aagcaccccg ccgacatccc cgactacaag aagctgtcct tccccgaggg
     3121 cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg tgacccagga
     3181 ctcctccctg caggacggca ccttcatcta ccacgtgaag ttcatcggcg tgaacttccc
     3241 ctccgacggc cccgtaatgc agaagaagac tctgggctgg gagccctcca ccgagcgcct
     3301 gtacccccgc gacggcgtgc tgaagggcga gatccacaag gcgctgaagc tgaagggcgg
     3361 cggccactac ctggtggagt tcaagtcaat ctacatggcc aagaagcccg tgaagctgcc
     3421 cggctactac tacgtggact ccaagctgga catcacctcc cacaacgagg actacaccgt
     3481 ggtggagcag tacgagcgcg ccgaggcccg ccaccacctg ttccagtccg gactcagatc
     3541 tcgagctgat ccaaaaaaga agagaaaggt agatccaaaa aagaagagaa aggtagatcc
     3601 aaaaaagaag agaaaggtag gatccaccgg atctagataa ctgatcagct agctatcagg
     3661 atcctacgag gcgcgccaga tagaattctc ttgggcctca agggccatga gttaaccagt
     3721 tctagatgtg actcatgtca attcgatatc aagcttatcg ataatcaacc tctggattac
     3781 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
     3841 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
     3901 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
     3961 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
     4021 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
     4081 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
     4141 gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg
     4201 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
     4261 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg
     4321 agtcggatct ccctttgggc cgcctccccg catcgatacc gtcgacctcg agacctagaa
     4381 aaacatggag caatcacaag tagcaataca gcagctacca atgctgattg tgcctggcta
     4441 gaagcacaag aggaggagga ggtgggtttt ccagtcacac ctcaggtacc tttaagacca
     4501 atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa
     4561 gggctaattc actcccaacg aagacaagat atccttgatc tgtggatcta ccacacacaa
     4621 ggctacttcc ctgattggca gaactacaca ccagggccag ggatcagata tccactgacc
     4681 tttggatggt gctacaagct agtaccagtt gagcaagaga aggtagaaga agccaatgaa
     4741 ggagagaaca cccgcttgtt acaccctgtg agcctgcatg ggatggatga cccggagaga
     4801 gaagtattag agtggaggtt tgacagccgc ctagcatttc atcacatggc ccgagagctg
     4861 catccggact gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
     4921 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     4981 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     5041 tggaaaatct ctagcagggc ccgtttaaac ccgctgatca gcctcgactg tgccttctag
     5101 ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac
     5161 tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca
     5221 ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag
     5281 caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg
     5341 ctctaggggg tatccccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
     5401 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
     5461 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
     5521 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga
     5581 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
     5641 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt
     5701 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct
     5761 gatttaacaa aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga
     5821 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     5881 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     5941 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
     6001 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     6061 gccgcctctg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
     6121 ttttgcaaaa agctcccggg agcttgtata tccattttcg gatctgatca gcacgtgttg
     6181 acaattaatc atcggcatag tatatcggca tagtataata cgacaaggtg aggaactaaa
     6241 ccatggccaa gttgaccagt gccgttccgg tgctcaccgc gcgcgacgtc gccggagcgg
     6301 tcgagttctg gaccgaccgg ctcgggttct cccgggactt cgtggaggac gacttcgccg
     6361 gtgtggtccg ggacgacgtg accctgttca tcagcgcggt ccaggaccag gtggtgccgg
     6421 acaacaccct ggcctgggtg tgggtgcgcg gcctggacga gctgtacgcc gagtggtcgg
     6481 aggtcgtgtc cacgaacttc cgggacgcct ccgggccggc catgaccgag atcggcgagc
     6541 agccgtgggg gcgggagttc gccctgcgcg acccggccgg caactgcgtg cacttcgtgg
     6601 ccgaggagca ggactgacac gtgctacgag atttcgattc caccgccgcc ttctatgaaa
     6661 ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc
     6721 tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat ggttacaaat
     6781 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     6841 gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc tctagctaga
     6901 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     6961 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     7021 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     7081 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     7141 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     7201 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     7261 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     7321 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     7381 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     7441 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     7501 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     7561 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     7621 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     7681 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     7741 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     7801 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     7861 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     7921 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     7981 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     8041 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     8101 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     8161 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     8221 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     8281 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     8341 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     8401 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     8461 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     8521 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     8581 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     8641 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     8701 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     8761 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     8821 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     8881 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     8941 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     9001 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     9061 tgccacctga cgtcgacgga tcgggagatc tcccgatccc ctatggtgca ctctcagtac
     9121 aatctgctct gatgccgcat agttaagcca gtatctgctc cctgcttgtg tgttggaggt
     9181 cgctgagtag tgcgcgagca aaatttaagc tacaacaagg caaggcttga ccgacaattg
     9241 catgaagaat ctgcttaggg ttaggcgttt tgcgctgctt cg
//
