LOCUS       sequence-274414-        4725 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses HA-tagged human CHMP6 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4725)
  TITLE     Mammalian expression plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4725)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: HA-CHMP6
FEATURES             Location/Qualifiers
     source          1..4725
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..978
                     /label=CMV IE94 promoter
                     /note="enhancer/promoter region of simian cytomegalovirus 
                     major immediate early transcription unit IE94"
     promoter        1013..1031
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     1013..1030
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             1070..1096
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(1731..1750)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1732..1750)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    1755..1889
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1779..1798)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1833..1852
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(2002..2022)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(2002..2020)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2041..2057)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2041..2057)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2054..2076)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2065..2081
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2089..2095,2096..2113,2114..2119))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2134..2155
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2272..2289)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2443..3031)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2523..2542)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3202..3993,3994..4062))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3825..3844
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4063..4167)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(4193..4648)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4330..4351)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4542..4561
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gccaattcaa tatggcgtat atggactcat gccaattcaa tatggtggat ctggacctgt
       61 gccaattcaa tatggcgtat atggactcgt gccaattcaa tatggtggat ctggacccca
      121 gccaattcaa tatggcggac ttggcaccat gccaattcaa tatggcggac ctggcactgt
      181 gccaactggg gaggggtcta cttggcacgg tgccaagttt gaggaggggt cttggccctg
      241 tgccaagtcc gccatattga attggcatgg tgccaataat ggcggccata ttggctatat
      301 gccaggatca atatataggc aatatccaat atggccctat gccaatatgg ctattggcca
      361 ggttcaatac tatgtattgg ccctatgcca tatagtattc catatatggg ttttcctatt
      421 gacgtagata gcccctccca atgggcggtc ccatatacca tatatggggc ttcctaatac
      481 cgcccatagc cactccccca ttgacgtcaa tggtctctat atatggtctt tcctattgac
      541 gtcatatggg cggtcctatt gacgtatatg gcgcctcccc cattgacgtc aattacggta
      601 aatggcccgc ctggctcaat gcccattgac gtcaatagga ccacccacca ttgacgtcaa
      661 tgggatggct cattgcccat tcatatccgt tctcacgccc cctattgacg tcaatgacgg
      721 taaatggccc acttggcagt acatcaatat ctattaatag taacttggca agtacattac
      781 tattggaagt acgccagggt acattggcag tactcccatt gacgtcaatg gcggtaaatg
      841 gcccgcgatg gctgccaagt acatccccat tgacgtcaat ggggaggggc aatgacgcaa
      901 atgggcgttc cattgacgta aatgggcggt aggcgtgcct aatgggaggt ctatataagc
      961 aatgctcgtt tagggaaccg ccattctgcc tggggacgtc ggagcaagct tgatttaggt
     1021 gacactatag aatacaagct acttgttctt tttgcaggat accatgcgat acccatatga
     1081 tgtgccagat tacgctggat ctatgggtaa cctgttcggc cgcaagaagc agagccgcgt
     1141 cacggagcag gacaaggcca tcctgcaact gaagcagcag cgggacaagc tgaggcagta
     1201 ccagaagagg atcgcccagc agctggagcg cgagcgcgcc ctggcccggc agctgctgcg
     1261 ggacggcagg aaggaacggg ccaagctgct gctcaagaag aagcgatacc aggagcagct
     1321 cctggacagg acggagaacc agatcagcag cctggaggcc atggttcaga gtattgagtt
     1381 cacccagatc gaaatgaaag tgatggaggg gctgcagttt ggaaatgagt gtctgaacaa
     1441 gatgcaccag gtgatgtcca ttgaagaggt ggagaggatc ctggacgaga cgcaggaggc
     1501 cgtggagtac cagcggcaaa tagacgagct cctggcagga agcttcactc aggaggatga
     1561 agacgccatc ctggaggagc tgagcgcaat cactcaggaa caaatagagc tgccagaggt
     1621 tccctccgag ccccttcctg agaagatccc agaaaacgtc cctgtcaagg ccaggcccag
     1681 gcaggcggag ctggtggcag cttcgtaact cgagcctcta gattctgcag ccctatagtg
     1741 agtcgtatta cgtagatcca gacatgataa gatacattga tgagtttgga caaaccacaa
     1801 ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg
     1861 taaccattat aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc
     1921 aggttcaggg ggaggtgtgg gaggtttttt aattcgcggc cgcggcgcca atgcattggg
     1981 cccggtaccc agcttttgtt ccctttagtg agggttaatt gcgcgcttgg cgtaatcatg
     2041 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     2101 cgggagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     2161 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     2221 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     2281 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     2341 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     2401 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     2461 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     2521 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     2581 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     2641 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     2701 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     2761 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     2821 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     2881 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     2941 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     3001 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     3061 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     3121 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     3181 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     3241 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     3301 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     3361 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     3421 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     3481 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     3541 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     3601 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     3661 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     3721 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     3781 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     3841 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     3901 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     3961 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     4021 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     4081 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     4141 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctaaattgta
     4201 agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac
     4261 caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg
     4321 agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa
     4381 gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt
     4441 tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt
     4501 agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga
     4561 gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc
     4621 gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg caactgttgg
     4681 gaagggcgat cggtgcgggc ctcttcgcta ttacgccagt cgacc
//
