LOCUS       sequence-274429-        8906 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CAG tdGFP dre/rox reporter piggyBac transposon (bpA 3x SV40pA stop).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8906)
  AUTHORS   Nam HS, Capecchi MR
  TITLE     piggyBac transposon plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8906)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: PB CAG-R-bpA-3xSV40pA-R-td-sfGFP
FEATURES             Location/Qualifiers
     source          1..8906
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        137..516
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..795
                     /label=chicken beta-actin promoter
     intron          796..1812
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1736..1758
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1820..1839
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    1867..1898
                     /label=rox
                     /bound_moiety="Dre recombinase"
     polyA_signal    1925..2149
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     polyA_signal    2334..2455
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    2567..2688
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    2800..2921
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    2944..2975
                     /label=rox
                     /bound_moiety="Dre recombinase"
     CDS             join(2984..2986,2987..3697)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             2984..3697
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Nager et al., 2011)"
                     /label=superfolder GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             join(3728..3730,3731..4444)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             3728..4441
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Nager et al., 2011)"
                     /label=superfolder GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(4502..4522)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4920..4931)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    5082..5306
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   5660..5694
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(5781..5800)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5782..5800)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5807..5824)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5807..5823)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5816..5838)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(5965..6420)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6102..6123)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6314..6333
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        6447..6551
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6552..6620,6621..7412)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6770..6789)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7583..8171
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8072..8091
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8325..8342
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8459..8480
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8495..8500,8501..8518,8519..8525)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8533..8549
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8538..8560
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8557..8573
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8557..8573
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8592..8612
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8594..8612
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     repeat_region   8676..8738
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
ORIGIN
        1 acttttaaac attctctctt ttacaaaaat aaacttattt tgtactttaa aaacagtcat
       61 gttgtattat aaaataagta attagcttaa cctatacata atagaaacaa attatactta
      121 ttaaccggtc atggtcgaca ttgattattg actagttatt aatagtaatc aattacgggg
      181 tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg
      241 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata
      301 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc
      361 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac
      421 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg
      481 cagtacatct acgtattagt catcgctatt accatggtcg aggtgagccc cacgttctgc
      541 ttcactctcc ccatctcccc cccctcccca cccccaattt tgtatttatt tattttttaa
      601 ttattttgtg cagcgatggg ggcggggggg gggggggggc gcgcgccggg gggggggggg
      661 gggggggggg gggggggsgg ggsgrggcgg rgaggtgcgg cggcagccaa tcagagcggc
      721 gcgctccgaa agtttccttt tatggcgagg cggcggcggc ggcggcccta taaaaagcga
      781 agcgcgcggc gggcgggagt cgctgcgcgc tgccttcgcc ccgtgccccg ctccgccgcc
      841 gcctcgcgcc gcccgccccg gctctgactg accgcgttac tcccacaggt gagcgggcgg
      901 gacggccctt ctcctccggg ctgtaattag cgcttggttt aatgacggct tgtttctttt
      961 ctgtggctgc gtgaaagcct tgaggggctc cgggagggcc ctttgtgcgg ggggagcggc
     1021 tcggggggtg cgtgcgtgtg tgtgtgcgtg gggagcgccg cgtgcggctc cgcgctgccc
     1081 ggcggctgtg agcgctgcgg gcgcggcgcg gggctttgtg cgctccgcag tgtgcgcgag
     1141 gggagcgcgg ccgggggcgg tgccccgcgg tgcggggggg gctgcgaggg gaacaaaggc
     1201 tgcgtgcggg gtgtgtgcgt gggggggtga gcagggggtg tgggcgcgtc ggtcgggctg
     1261 caaccccccc tgcacccccc tccccgagtt gctgagcacg gcccggcttc gggtgcgggg
     1321 ctccgtacgg ggcgtggcgc ggggctcgcc gtgccgggcg gggggtggcg gcaggtgggg
     1381 gtgccgggcg gggcggggcc gcckcgggcc ggggrgggct cgggggrggg gcgcggcggc
     1441 ccccggagcg ccggcggctg tcgaggcgcg gcgagccgca gccattgcct tttatggtaa
     1501 tcgtgcgaga gggcgcaggg acttcctttg tcccaaatct gtgcggagcc gaaatctggg
     1561 aggcgccgcc gcaccccctc tagcgggcgc ggggcgaagc ggtgcggcgc cggcaggaag
     1621 gaaatgggcg gggagggcct tcgtgcgtcg ccgcgccgcc gtccccttct ccctctccag
     1681 cctcggggct gtccgcgggg ggacggctgc cttcgggggg gacggggcag ggcggggttc
     1741 ggcttctggc gtgtgaccgg cggctctaga gcctctgcta accatgttca tgccttcttc
     1801 tttttcctac agctcctggg caacgtgctg gttattgtgc tgtctcatca ttttggcaaa
     1861 gaattctaac tttaaataat tggcattatt taaagttagc tagcagctcg ctgatcagcc
     1921 tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg
     1981 accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat
     2041 tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag
     2101 gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg
     2161 gaaagaacca gctggggctc gactagagct tgcggaaccc ttaccaccgc ggtggcggcc
     2221 gctctagaat cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc
     2281 tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt
     2341 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     2401 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttaactag
     2461 aatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca
     2521 cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc
     2581 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     2641 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttaac tagaatcata
     2701 atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc
     2761 ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat
     2821 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     2881 cattctagtt gtggtttgtc caaactcatc aatgtatctt aactagtgga tctcatgcct
     2941 aggtaacttt aaataattgg cattatttaa agttaggcgc gccatgagca aaggagaaga
     3001 acttttcact ggagttgtcc caattcttgt tgaattagat ggtgatgtta atgggcacaa
     3061 attttctgtc cgtggagagg gtgaaggtga tgctacaaac ggaaaactca cccttaaatt
     3121 tatttgcact actggaaaac tacctgttcc gtggccaaca cttgtcacta ctctgaccta
     3181 tggtgttcaa tgcttttccc gttatccgga tcacatgaaa cggcatgact ttttcaagag
     3241 tgccatgccc gaaggttatg tacaggaacg cactatatct ttcaaagatg acgggaccta
     3301 caagacgcgt gctgaagtca agtttgaagg tgataccctt gttaatcgta tcgagttaaa
     3361 gggtattgat tttaaagaag atggaaacat tcttggacac aaactcgaat acaactttaa
     3421 ctcacacaat gtatacatca cggcagacaa acaaaagaat ggaatcaaag ctaacttcaa
     3481 aattcgccac aacgttgaag atggttccgt tcaactagca gaccattatc aacaaaatac
     3541 tccaattggc gatggccctg tccttttacc agacaaccat tacctgtcga cacaatctgt
     3601 cctttcgaaa gatcccaacg aaaagcgtga ccacatggtc cttcttgagt ttgtaactgc
     3661 tgctgggatt acacatggca tggatgagct ctacaaaggt tctgctggat ctgcagcagg
     3721 atctggtatg agcaaaggag aagaactttt cactggagtt gtcccaattc ttgttgaatt
     3781 agatggtgat gttaatgggc acaaattttc tgtccgtgga gagggtgaag gtgatgctac
     3841 aaacggaaaa ctcaccctta aatttatttg cactactgga aaactacctg ttccgtggcc
     3901 aacacttgtc actactctga cctatggtgt tcaatgcttt tcccgttatc cggatcacat
     3961 gaaacggcat gactttttca agagtgccat gcccgaaggt tatgtacagg aacgcactat
     4021 atctttcaaa gatgacggga cctacaagac gcgtgctgaa gtcaagtttg aaggtgatac
     4081 ccttgttaat cgtatcgagt taaagggtat tgattttaaa gaagatggaa acattcttgg
     4141 acacaaactc gaatacaact ttaactcaca caatgtatac atcacggcag acaaacaaaa
     4201 gaatggaatc aaagctaact tcaaaattcg ccacaacgtt gaagatggtt ccgttcaact
     4261 agcagaccat tatcaacaaa atactccaat tggcgatggc cctgtccttt taccagacaa
     4321 ccattacctg tcgacacaat ctgtcctttc gaaagatccc aacgaaaagc gtgaccacat
     4381 ggtccttctt gagtttgtaa ctgctgctgg gattacacat ggcatggatg agctctacaa
     4441 atgattaatt aaaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact
     4501 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
     4561 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg
     4621 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa
     4681 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc
     4741 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
     4801 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt
     4861 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt
     4921 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc
     4981 cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcagg
     5041 atccactaga gcggccgcct agagctcgct atcagcctcg actgtgcctt ctagttgcca
     5101 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac
     5161 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat
     5221 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca
     5281 tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctcgac
     5341 tagagcttgc ggaaccctta agctttttta ttatatatta gtcacgatat ctataacaag
     5401 aaaatatata tataataagt tatcacgtaa gtagaacatg aaataacaat ataattatcg
     5461 tatgagttaa atcttaaaag tcacgtaaaa gataatcatg cgtcattttg actcacgcgg
     5521 tcgttatagt tcaaaatcag tgacacttac cgcattgaca agcacgcctc acgggagctc
     5581 caagcggcga ctgagatgtc ctaaatgcac agcgacggat tcgcgctatt tagaaagaga
     5641 gagcaatatt tcaagaatgc atgcgtcaat tttacgcaga ctatctttct agggttaaaa
     5701 aagatttgcg ctttactcga cctaaacttt aaacacgtca tagaatcttc gtttgacaaa
     5761 aaccacattg tgttgggtcg ccctatagtg agtcgtatta caattcactg gccgtcgttt
     5821 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc
     5881 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
     5941 tgcgcagcct gaatggcgaa tggcaaattg taagcgttaa tattttgtta aaattcgcgt
     6001 taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt
     6061 ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc
     6121 cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg
     6181 gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac
     6241 taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg
     6301 tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag
     6361 cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt
     6421 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     6481 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     6541 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     6601 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     6661 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     6721 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     6781 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     6841 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     6901 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     6961 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     7021 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     7081 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     7141 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     7201 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     7261 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     7321 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     7381 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     7441 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     7501 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     7561 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     7621 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     7681 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     7741 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     7801 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     7861 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     7921 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     7981 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     8041 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     8101 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     8161 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     8221 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     8281 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     8341 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     8401 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     8461 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     8521 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
     8581 acgccaagcg cgcaattaac cctcactaaa gggaacctcc cccggtattc acgacagcag
     8641 gctgaataat aaaaaaatta gaaactatta tttaacccta gaaagataat catattgtga
     8701 cgtacgttaa agataatcat gcgtaaaatt gacgcatgtg ttttatcggt ctgtatatcg
     8761 aggtttattt attaatttga atagatatta agttttatta tatttacact tacatactaa
     8821 taataaattc aacaaacaat ttatttatgt ttatttattt attaaaaaaa aacaaaaact
     8881 caaaatttct tctataaagt aacaaa
//
