LOCUS       sequence-274450-        6105 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses only the hyperactive form of the ADAR catalytic domain - 
            no RNA binding protein. This is a control plasmid to be used with 
            pMT-Hrp48-ADARcd-E488Q-V5.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6105)
  AUTHORS   Xu W, Rahman R, Rosbash M
  TITLE     Mechanistic implications of enhanced editing by a HyperTRIBE 
            RNA-binding protein.
  JOURNAL   RNA. 2018 Feb;24(2):173-182. doi: 10.1261/rna.064691.117. Epub 2017 
            Nov 10.
  PUBMED    29127211
REFERENCE   2  (bases 1 to 6105)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.064691.117"; journalName: "RNA"; date: "2018-02"; 
            volume: "24"; issue: "2"; pages: "173-182"
COMMENT     Sequence Label: pMT-ADARcd-E488Q-V5
FEATURES             Location/Qualifiers
     source          1..6105
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     364..386
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     378..395
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        401..827
                     /label=MT promoter
                     /note="Drosophila metallothionein promoter"
     CDS             2263..2304
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             2314..2331
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(2354..2371)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2373..2507
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2651..2667)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2651..2667)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2664..2686)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2675..2691
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2699..2705,2706..2723,2724..2729))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2744..2765
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        2950..3229
                     /label=copia promoter
                     /note="strong promoter from the Drosophila transposable 
                     element copia (Sinclair et al., 1986)"
     CDS             3246..3644
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    3973..4107
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4115..4132)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4286..4874)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4366..4385)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5045..5836,5837..5905))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5668..5687
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5906..6010)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6078..6096
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt aattcgttgc aggacaggat
      421 gtggtgcccg atgtgactag ctctttgctg caggccgtcc tatcctctgg ttccgataag
      481 agacccagaa ctccggcccc ccaccgccca ccgccacccc catacatatg tggtacgcaa
      541 gtaagagtgc ctgcgcatgc cccatgtgcc ccaccaagag ctttgcatcc catacaagtc
      601 cccaaagtgg agaaccgaac caattcttcg cgggcagaac aaaagcttct gcacacgtct
      661 ccactcgaat ttggagccgg ccggcgtgtg caaaagaggt gaatcgaacg aaagacccgt
      721 gtgtaaagcc gcgtttccaa aatgtataaa accgagagca tctggccaat gtgcatcagt
      781 tgtggtcagc agcaaaatca agtgaatcat ctcagtgcaa ctaaaggggg gatctagatc
      841 ggggtacctc gaaggccgcc atatgctagc aatggtagcg gccgccaccg cggtggacta
      901 cagtccaatg gtggtgccac agaagaacgt acccctgcca atcgacgaca agtcgtcatc
      961 gatggagttg cctcagatac acgcggatac gattggtcgg ttggtcttag aaaagttcat
     1021 ggaagtaatc aagggccagg aggcttactc gcgtcgaaag gtattagcgg gcattgtaat
     1081 gactgaaaac atgaattttt gtgaagccaa agttatttca gtttcgacgg gcaccaagtg
     1141 tgtcagcggt gagcatatga gtgtgaacgg agctgtccta aatgattccc atgctgaaat
     1201 agtctccagg cgttgtctcc tcaaatattt atatgcacag ctggaccttc agtgcaatca
     1261 ggccacagca tatcagtcga ttttcgtgag gaatactgat gggcaatacc cttataaact
     1321 aaaatccggg gtacatttcc atttgtatat aaatacagca ccttgtgggg atgcacggat
     1381 atttagtcct cacgaaaacg acactggtgt tgataaacat ccaaatagaa aagctcgagg
     1441 ccagctgcgc accaaaatcg agtccggtca ggggacgatt ccagtgaaaa gcagtgatgg
     1501 tatccagacg tgggatggcg tattgcaggt aacccgattg ggcggttgct attagatcgt
     1561 ggtaaaccat caacattaca acatttatga cttttgtgca gggccaacgc ctgctaacaa
     1621 tgtcgtgctc ggataaaatt gcccgttgga acatcgtggg catccaaggc tcactattgt
     1681 cttccataat tgaaccggtg tacctgcatt cgattgtgct gggaagcctg ttgcatccgg
     1741 agcacatgta ccgcgcagtt tgcggccgaa ttgagaagtc cattcaaggc ctgccaccgc
     1801 cgtaccatct gaacaagccg cgcctggcac tagtcacttc ggccgagccg cggaatcagg
     1861 ccaaggctcc caacttcggg atcaattgga ccattgggga caccgagctg gaagtggtga
     1921 actcgctaac cggtcgaacc attggcggcc aggtgtctcg catcacaaag caggcgtttt
     1981 tcgttaagta tggatttcta atggcaaact tgcccggtat tttagtccgc aaagtaacca
     2041 ctgactatgg gcaaaccaaa gctaacgtta aggactatca ggtgagtgtt tatatgctgg
     2101 atcatttgag gatatgcatc aaattgtttg ccacatctgt tcttgtagat cgcaaagcta
     2161 gaattgttct ctgcattcaa gcgagaagac cttggcagct ggctgaagaa acccattgaa
     2221 caagacgagt tcggtcttgc cgaatctaga gggcccttcg aaggtaagcc tatccctaac
     2281 cctctcctcg gtctcgattc tacgcgtacc ggtcatcatc accatcacca ttgagtttaa
     2341 acccgctgat cagcctcgac tgtgccttct aagatccaga catgataaga tacattgatg
     2401 agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg
     2461 atgctattgc tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt
     2521 gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttaa agcaagtaaa
     2581 acctctacaa atgtggtatg gctgattatg atcagtcgac ctgcaggcat gcaagcttgg
     2641 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
     2701 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
     2761 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
     2821 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgcta
     2881 tccggtgcgt ggtggtttca tgcttctggg aacggcaaat gggtttagga ttgggaaccc
     2941 ctcatcatct gttggaatat actattcaac ctacaaaagt aacgttaaac aacactactt
     3001 tatatttgat atgaatggcc acacctttta tgccataaaa catattgtaa gagaatacca
     3061 ctctttttat tccttctttc cttcttgtac gttttttgct gtaagtaggt cgtggtgctg
     3121 gtgttgcagt tgaaataact taaaatataa atcataaaac tcaaacataa acttgactat
     3181 ttatttattt attaagaaag gaaatataaa ttgtaaatta caacaggttc cctttatgcg
     3241 aagggatggc caagcctttg tctcaagaag aatccaccct cattgaaaga gcaacggcta
     3301 caatcaacag catccccatc tctgaagact acagcgtcgc cagcgcagct ctctctagcg
     3361 acggccgcat cttcactggt gtcaatgtat atcattttac tgggggacct tgcgcagaac
     3421 tcgtggtgct gggcactgct gctgctgcgg cagctggcaa cctgacttgt atcgtcgcga
     3481 tcggaaatga gaacaggggc atcttgagcc cctgcggacg gtgccgacag gttcttctcg
     3541 atctgcatcc tgggatcaaa gccatagtga aggacagtga tggacagccg acggcagttg
     3601 ggattcgtga attgctgccc tctggttatg tgtgggaggg ctaaccctta tacgcaaggg
     3661 agtagatgcc gaccgaacaa gagctgattt cgagaacgcc tcagccagca actcgcgcga
     3721 gcctagcaag gcaaatgcga gagaacggcc ttacgcttgg tggcacagtt ctcgtccaca
     3781 gttcgctaag ctcgctcggc tgggtcgcgg gagggccggt cgcagtgatt caggcccttc
     3841 tggattgtgt tggtccccag ggcacgattg tcatgcccac gcactcgggt gatctgactg
     3901 atcccgcaga ttggagatcg ccgcccgtgc ctgccgattg ggtgcagatc agcctcgagg
     3961 ccagctagct tgaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     4021 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     4081 atcaatgtat cttatcatgt ctggatcggg cttcctcgct cactgactcg ctgcgctcgg
     4141 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     4201 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     4261 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     4321 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     4381 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     4441 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     4501 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     4561 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     4621 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     4681 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     4741 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     4801 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     4861 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     4921 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     4981 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     5041 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     5101 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     5161 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     5221 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     5281 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     5341 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     5401 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     5461 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     5521 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     5581 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     5641 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     5701 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     5761 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     5821 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     5881 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     5941 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     6001 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca
     6061 tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtc
//
