LOCUS       sequence-274457-        9515 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For in vivo tagging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9515)
  AUTHORS   Hu X, Peng WX, Zhou H, Jiang J, Zhou X, Huang D, Mo YY, Yang L
  TITLE     IGF2BP2 regulates DANCR by serving as an N6-methyladenosine reader.
  JOURNAL   Cell Death Differ. 2019 Dec 5. pii: 10.1038/s41418-019-0461-z. doi: 
            10.1038/s41418-019-0461-z.
  PUBMED    31804607
REFERENCE   2  (bases 1 to 9515)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Death 
            Differ. 2019 Dec 5. pii: 10.1038/s41418-019-0461-z. doi: 
            10.1038/s41418-019-0461-z."
COMMENT     Sequence Label: S1m tagged DANCR
FEATURES             Location/Qualifiers
     source          1..9515
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        6..232
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             233..413
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    460..585
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1078..1311
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1496..1540
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1807..1923
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3657..3868
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             3881..4149
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     regulatory      4178..4187
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4208..4870
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina 
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /label=CopGFP
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
     CDS             4940..4993
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             4994..5593
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4994..5013)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5490..5510
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    5594..6182
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5647..5667)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6065..6076)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6256..6489
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6561..6682
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6598..6617)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6652..6671
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6722..6857
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6890..6906)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6890..6906)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6903..6925)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6914..6930
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6938..6944,6945..6962,6963..6968))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6983..7004
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7121..7138)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7292..7880)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(8051..8842,8843..8911))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8674..8693
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8912..9016)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9084..9102
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(9140..9162)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     9262..9281
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9475..9497
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9489..9506
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9490..9506
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacataa acgggtctct
      241 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
      301 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
      361 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc
      421 ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg
      481 cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt
      541 ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg
      601 agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa
      661 ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg
      721 ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca
      781 ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa
      841 aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa
      901 agtaagacca ccgcacagca agcggccact gatcttcaga cctggaggag gagatatgag
      961 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt
     1021 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg
     1081 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac
     1141 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct
     1201 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct
     1261 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg
     1321 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa
     1381 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa
     1441 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa
     1501 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac
     1561 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag
     1621 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc
     1681 gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga
     1741 aggtggagag agagacagag acagatccat tcgattagtg aacggatctc gacggtatcg
     1801 gttaactttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga
     1861 cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa aattcaaaat
     1921 tttatcgata ctagttgaaa gaccccacct gtaggtttgg caagttagct taagtaacgc
     1981 cattttgcaa ggcatggaaa atacataact gagaatagag aagttcagat caaggttagg
     2041 aacagagaga cagcagaata tgggccaaac aggatatctg tggtaagcag ttcctgcccc
     2101 ggctcagggc caagaacaga tggtccccag atgcggtccc gccctcagca gtttctagcg
     2161 aaccatcaga tgtttccagg gtgccccaag gacctgaaat gaccctgtgc cttatttgaa
     2221 ctaaccaatc agtttgcttc ttgcttctgt ttgtgtgctt ctgctccctg agctcaataa
     2281 aagagcccac aacccctcac ttggtgggcc agtcctctga tagactgtgt cccctggata
     2341 cccgtattct agagctagcg aattcgccct tgcccagagt cttcccggga ttggctgcgg
     2401 gcctcgcgac cctcctgctt ccctccccgc cccgcgccgc ctctctggtt tgtgcgcccg
     2461 tcgcaggtcg caggcctctt tgtcagctgg agttgcgcgg gctgacgcgc cactatgtag
     2521 cgggtttcgg gcgggccacg cgtgcgggac aggaacccaa ccccagccga ccttgagctc
     2581 caggagttcg tctcttacgt ctgcggaagt gcagctgcct cagttcttag cgcaggttga
     2641 caactacagg cacaagccat tgaagctgga atgtcctgtt gctggtattt caattgactt
     2701 aagccaacta tcccttcagt tacaatagga aagtgcctct aataaggcca aatatgcgta
     2761 ctaacttgta gcaaccacgt gtccgtgcag tgccacagga gctagagcag tgacaatgct
     2821 ggtggcaaca gggcagtgta gcaggtgctt catgttcacc ttttcaacct tttcatttaa
     2881 ttgtcacaac tcggaggtgg attctgttag ggacaggctg ccccaggacc actccgcccc
     2941 cgctaactca atgcagctga cccttaccct gaatactctg cagctgcatt cctgaaccgt
     3001 tatctaggcg ctatagcaag gtcaccagac ttgctacacc gaagccctct gggtggcacg
     3061 ggggaggtca tgagaaacgt ggattacacc cccttgtaaa ttcctatttt cacaagataa
     3121 tatattgtaa gccggtcatg agattatatg tggtaaagtt aattgactaa caaccccagg
     3181 gtctctctcc cccatataaa cccctcattt tgtaagctca gggctgccac ctccgactgg
     3241 tggagaagcc tggcaggtta ataaacttac ttggcctgac ggatccggat ccgtagaaaa
     3301 tgcggccgcc gaccagaatc atgcaagtgc gtaagatagt cgcgggtcgg cggccgcatc
     3361 tgctgggaga tccgtagaaa atgcggccgc cgaccagaat catgcaagtg cgtaagatag
     3421 tcgcgggtcg gcggccgcat ctgctgggag atccgtagaa aatgcggccg ccgaccagaa
     3481 tcatgcaagt gcgtaagata gtcgcgggtc ggcggccgca tctgctggga gatccgtaga
     3541 aaatgcggcc gccgaccaga atcatgcaag tgcgtaagat agtcgcgggt cggcggccgc
     3601 atctgctggg agatctgcgg ccgcaaggat ctgcgatcgc tccggtgccc gtcagtgggc
     3661 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaacggg
     3721 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct
     3781 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt
     3841 tcgcaacggg tttgccgcca gaacacagct gaagcttcga ggggctcgca tctctccttc
     3901 acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg ttctgccgcc
     3961 tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa agctcaggtc
     4021 gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc ggctctccac
     4081 gctttgcctg accctgcttg ctcaactcta cgtctttgtt tcgttttctg ttctgcgccg
     4141 ttacagatcc aagctgtgac cggcgcctac gctagacgcc accatggaga gcgacgagag
     4201 cggcctgccc gccatggaga tcgagtgccg catcaccggc accctgaacg gcgtggagtt
     4261 cgagctggtg ggcggcggag agggcacccc caagcagggc cgcatgacca acaagatgaa
     4321 gagcaccaaa ggcgccctga ccttcagccc ctacctgctg agccacgtga tgggctacgg
     4381 cttctaccac ttcggcacct accccagcgg ctacgagaac cccttcctgc acgccatcaa
     4441 caacggcggc tacaccaaca cccgcatcga gaagtacgag gacggcggcg tgctgcacgt
     4501 gagcttcagc taccgctacg aggccggccg cgtgatcggc gacttcaagg tggtgggcac
     4561 cggcttcccc gaggacagcg tgatcttcac cgacaagatc atccgcagca acgccaccgt
     4621 ggagcacctg caccccatgg gcgataacgt gctggtgggc agcttcgccc gcaccttcag
     4681 cctgcgcgac ggcggctact acagcttcgt ggtggacagc cacatgcact tcaagagcgc
     4741 catccacccc agcatcctgc agaacggggg ccccatgttc gccttccgcc gcgtggagga
     4801 gctgcacagc aacaccgagc tgggcatcgt ggagtaccag cacgccttca agacccccat
     4861 cgccttcgcc agatcccgcg ctcagtcgtc caattctgcc gtggacggca ccgccggacc
     4921 cggctccacc ggatctcgcg agggcagagg aagtcttcta acatgcggtg acgtggagga
     4981 gaatcccggc cctatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt
     5041 ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac
     5101 cgtcgatccg gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg
     5161 cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg
     5221 gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc
     5281 cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca
     5341 ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg
     5401 caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt
     5461 gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt
     5521 caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa
     5581 gcccggtgcc tgaaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct
     5641 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc
     5701 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct
     5761 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga
     5821 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc
     5881 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac
     5941 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt
     6001 tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt
     6061 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc
     6121 tcttccgcgt ctccgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc
     6181 gcctggtacc tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
     6241 aagaaaaggg gggactggaa gggctaattc actcccaacg aagataagat ctgctttttg
     6301 cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     6361 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     6421 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     6481 tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat
     6541 gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca
     6601 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     6661 ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc
     6721 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     6781 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
     6841 aggctttttt ggaggcctag acttttgcag agaccaaatt cgtaatcatg tcatagctgt
     6901 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     6961 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     7021 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     7081 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     7141 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     7201 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     7261 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     7321 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     7381 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     7441 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     7501 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     7561 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     7621 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     7681 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     7741 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     7801 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     7861 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     7921 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     7981 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     8041 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     8101 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     8161 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     8221 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     8281 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     8341 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     8401 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     8461 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     8521 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     8581 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     8641 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     8701 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     8761 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     8821 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     8881 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     8941 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     9001 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta
     9061 ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt
     9121 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc
     9181 tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt
     9241 gtcggggctg gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc
     9301 ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc cattcgccat
     9361 tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc
     9421 tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt
     9481 cacgacgttg taaaacgacg gccagtgcca agctg
//
