LOCUS       sequence-274458-        6182 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6182)
  AUTHORS   Mosimann C, Kaufman CK, Li P, Pugach EK, Tamplin OJ, Zon LI
  TITLE     Ubiquitous transgene expression and Cre-based recombination driven 
            by the ubiquitin promoter in zebrafish.
  JOURNAL   Development. 2011 Jan . 138(1):169-77.
  PUBMED    21138979
REFERENCE   2  (bases 1 to 6182)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development. 2011 Jan . 138(1):169-77."
COMMENT     Sequence Label: pENTR5'_ubi
FEATURES             Location/Qualifiers
     source          1..6182
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    3386..3509
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3585..3604)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3586..3604)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3609..3625)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3609..3625)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3727..3746)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             3738..4547
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(3805..3824)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      4694..5282
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5183..5202
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5436..5453
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(5612..5639)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(5731..5817)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     5866..5888
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5880..5897
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5881..5897
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    5937..6032
                     /gene="mutant version of attL"
                     /label=attL4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 taacaaagca gtaggcttaa ataaacctaa tttttatagg ctgttctcta caaccctcaa
       61 acagtgatta gttttgtact tataaacttg ccctttcatt catatttcaa gaaaattggt
      121 tcagaagatc tggatattct agcagttgtt caagctcatg gagggatcag tgacctgatt
      181 ccacaatgac taggcctaat ccagaaatta gatgactgtc aacataaaaa ggcacagcac
      241 tcactagctg ccctatatat tttattatat tttacatata ttattttatt tatttagctc
      301 tgagtgctgt actttctggt taaagaaaac tgcttacaac agctaacctg tactacctca
      361 ggctcaggga atttggaaca ggtttgtctg gtttgtttct ttaaccatgc atgcttgttt
      421 tcaactatgg caacacagtc acatgggaca ttacagaaat gatttgtcga tgacatgcga
      481 cttttcttta ataaagcgca aagatcccaa aaagcaaact tttaacaaaa atcatataat
      541 tatattttca atccagcttt gtagcaactt tgtgctgctg ttcactcagc aacagatagt
      601 cagtataagg tcagtgtgtc tcaaagcagt gccatctgtt tcacacattg cgttctatat
      661 ataagtgtgc tggttgacac gacactgtat aaggcctagg ctaaaacaca aacaatgtag
      721 aatgacactg tgtttttttt gtaaacaaat gttgtttttg gttaaacatc tttgtgaaaa
      781 catcctcctg tcatgtattt gctatattca aatgttaaac ccgtgcagaa tagaacatat
      841 acaaaaaaaa acaacacaac acatttttaa acattattaa atatcaagta ttgctggcag
      901 ttctgtttct gttttacagt accctttgcc acagttctcc gcttttcctg gtccagattc
      961 cacaagtctg attcaccaat agcaaagcga ataaacaacc aaagcagcca atcactgctt
     1021 gtagactgtc ctgcgagacc ggcccattcc agcacattct ggaaacttcc tttatatgat
     1081 aattataaat acatttaaat tattgataca aaacatgtaa ttcctagaac ataaccatag
     1141 caatcattag ttttcagggt aattatgtat ttttaggatt tgactgcgga aagatctggt
     1201 catgtgacgt ctcatgaacg tcacggccct gggtttctat aaatacagta ggactctcga
     1261 ccatcggcag atttttcgaa gaagaagatc agtttcagga gccgtactgt tccgtttcaa
     1321 cgcaaatatt aacggtaaga gcgaatttcc tagtttgttt tcatgccatt ctttaaaacc
     1381 atagcgtatt actttaatta tagtaaactt tcgctttctt tattacaaga gacgttttgt
     1441 gttgattctc cgcggacatt ttcggtcaga caatcagaaa atgaccgcgg aggaccagta
     1501 acttgcatta cacgtaagtt aaatcttcgt gtattaaaat ggttaggttg ttaacgtcaa
     1561 ataggttacc gtgtttgcgt gtgatcaggt tggttttgtt agatttttgt cagtattttt
     1621 aatttatttg ttttagttta tttatttttt ttgctgaatc atagtttgtg aacaaagaac
     1681 ccggatgtta catacagtac agccgccatg ttacagagag ttataactta atcattttaa
     1741 aaataatttt gccttacttt tagtttgtca tgttgagaaa tgaggaaatg ttaaaatgag
     1801 gaaatatcca attaatttaa tatatcaaaa taatccatga ttacaatgca ctgaactgga
     1861 gaaaattaag atgttttcta gtgtcatgaa acaaatgtaa gagatgtaca ttgtagatgt
     1921 tttatgtcaa gaattggcta gttgatgcag catactggcg atactcagtt gtaataacag
     1981 taacgttaca tgttaataga ctactgagta tgctgttctg tctatgtatg ctctgtaagc
     2041 tacgagaagg acttttttaa acagtaaagg gtgcaatatt tttacaaatt gaattaaata
     2101 aaggctgtct attaagtaat atgcttgata tttttcttac ttgatcggaa ataagaaaaa
     2161 atataaacgt tgttgctcta aaaatcctag ttcagtttag ccaaccacaa ataccttttt
     2221 gttcctccaa cagttttttt ttcttctcta taatatttgg cagtctatag tactccaaat
     2281 gtttccccac agtctaacta attggtacag ccaaaatcat gacacttatt gcaataataa
     2341 ttttggttca ttggcattgt tgatagcctg tgccactaat atggtcgatt gatcatgctt
     2401 caggaagaaa actatattgt ttgatgtaag attattaaat cttcacctgc ttccattaca
     2461 aactattccc atcttattga attctggtat gtcttaaagg attagttcac ttcccaaatc
     2521 aaaatttact tagccttttt tcatccatga tccctttttt tcatcattaa tgaagaaatt
     2581 gtttttgaaa aagtttcaag atttttttct ctatattgtg gagcttgtta gtttaaaatt
     2641 ccaaaatgca atatgtggct tcaaatggtt ctaaatgatc ccagtcaagg aataacagtc
     2701 ttatctaatg aaaccattag acctttaaaa ataaaaaaaa taaaagtatt tatttttaaa
     2761 tgactgagtg attaagttga atttcagcgt ttccttactg tgtagaagtc cttccttact
     2821 ggccccaccc tttggttctc tgccaatctg ctacctaatg taatgttgtg gaacattatt
     2881 attctttatt tcttaatttt ttatttttta ttttaaaaca atgtaaactg cacagatgtg
     2941 cagtttgttt aaaatggcca atgctttgga aatgcatgac ataattagat ttcatgatgc
     3001 acaaagccaa atctcagagc ttgtgcaaaa tgagctatca tttcactagg taagacccta
     3061 aattttcata taggatcatt tggacaattt tgctgcaggt aaaatgcatt ctatagtcca
     3121 ctgtcagcca ttgttttgga tagtatttat ttttctctac aagtatagtc aatagttttc
     3181 tattatttta aaggtttgta acatttaagg gtgaccaaat gcaaagtaaa atttcatttt
     3241 cgggtgaact atctcgttta acatgggaga agtgcaaaac atacattatt ggctagaaca
     3301 ttgtagtatt ttttaaatgg aaatgtgtga ttgctaatct tactttgaat ttgtttacag
     3361 ggatccttta agggcgaatt cgacccaagt ttgtacaaaa aagttgaacg agaaacgtaa
     3421 aatgatataa atatcaatat attaaattag attttgcata aaaaacagac tacataatac
     3481 tgtaaaacac aacatatgca gtcactatga accaactact tagatggtat tagtgacctg
     3541 tagaattaat tcgagctcta gagctgcagg gcggccgcga tatcccctat agtgagtcgt
     3601 attacatggt catagctgtt tcctggcagc tctggcccgt gtctcaaaat ctctgatgtt
     3661 acattgcaca agataaaaat atatcatcat gaacaataaa actgtctgct tacataaaca
     3721 gtaatacaag gggtgttatg agccatattc aacgggaaac gtcgaggccg cgattaaatt
     3781 ccaacatgga tgctgattta tatgggtata aatgggctcg cgataatgtc gggcaatcag
     3841 gtgcgacaat ctatcgcttg tatgggaagc ccgatgcgcc agagttgttt ctgaaacatg
     3901 gcaaaggtag cgttgccaat gatgttacag atgagatggt cagactaaac tggctgacgg
     3961 aatttatgcc tcttccgacc atcaagcatt ttatccgtac tcctgatgat gcatggttac
     4021 tcaccactgc gatccccgga aaaacagcat tccaggtatt agaagaatat cctgattcag
     4081 gtgaaaatat tgttgatgcg ctggcagtgt tcctgcgccg gttgcattcg attcctgttt
     4141 gtaattgtcc ttttaacagc gatcgcgtat ttcgtctcgc tcaggcgcaa tcacgaatga
     4201 ataacggttt ggttgatgcg agtgattttg atgacgagcg taatggctgg cctgttgaac
     4261 aagtctggaa agaaatgcat aaacttttgc cattctcacc ggattcagtc gtcactcatg
     4321 gtgatttctc acttgataac cttatttttg acgaggggaa attaataggt tgtattgatg
     4381 ttggacgagt cggaatcgca gaccgatacc aggatcttgc catcctatgg aactgcctcg
     4441 gtgagttttc tccttcatta cagaaacggc tttttcaaaa atatggtatt gataatcctg
     4501 atatgaataa attgcagttt catttgatgc tcgatgagtt tttctaatca gaattggtta
     4561 attggttgta acactggcag agcattacgc tgacttgacg ggacggcgca agctcatgac
     4621 caaaatccct taacgtgagt tacgcgtcgt tccactgagc gtcagacccc gtagaaaaga
     4681 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     4741 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     4801 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     4861 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     4921 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     4981 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     5041 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     5101 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     5161 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     5221 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     5281 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     5341 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     5401 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     5461 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
     5521 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatacgcgta
     5581 ccgctagcca ggaagagttt gtagaaacgc aaaaaggcca tccgtcagga tggccttctg
     5641 cttagtttga tgcctggcag tttatggcgg gcgtcctgcc cgccaccctc cgggccgttg
     5701 cttcacaacg ttcaaatccg ctcccggcgg atttgtccta ctcaggagag cgttcaccga
     5761 caaacaacag ataaaacgaa aggcccagtc ttccgactga gcctttcgtt ttatttgatg
     5821 cctggcagtt ccctactctc gcgttaacgc tagcatggat gttttcccag tcacgacgtt
     5881 gtaaaacgac ggccagtctt aagctcgggc ccgagttaac gctaccatgg agctccaaat
     5941 aatgatttta ttttgactga tagtgacctg ttcgttgcaa caaattgata agcaatgctt
     6001 ttttataatg ccaactttgt atagaaaagt tggctccgaa ttcgccctta aaactcgaga
     6061 ccagcaaagt tctagaattt gtcgaaacat ttatgttata tatttcctga aaaaaattct
     6121 gagtaagttc ttaagtgtta ttgccagcaa cataaacaac agacggcaaa atgaataaat
     6181 ga
//
