LOCUS       sequence-274473-        8816 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Inducible Gli2 shRNA lentiviral plasmid (target sequence: human GLI2
            5′CCGGCCTGGCATGACTACCACTATGCTCGAGCATAGTGGTAGTCATGCCAGGTTTTTG 3′).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8816)
  AUTHORS   Zhu H, Xia L, Shen Q, Zhao M, Gu X, Bouamar H, Wang B, Sun LZ, Zhu X
  TITLE     Differential effects of GLI2 and GLI3 in regulating cervical cancer 
            malignancy in vitro and in vivo.
  JOURNAL   Lab Invest. 2018 Nov;98(11):1384-1396. doi: 
            10.1038/s41374-018-0089-5. Epub 2018 Jul 2.
  PUBMED    29967343
REFERENCE   2  (bases 1 to 8816)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41374-018-0089-5"; journalName: "Lab Invest"; date: 
            "2018-11"; volume: "98"; issue: "11"; pages: "1384-1396"
COMMENT     Sequence Label: pTet-GLI2shR
FEATURES             Location/Qualifiers
     source          1..8816
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(12..31)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     241..258
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     CDS             complement(460..1080)
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     988..1007
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     promoter        complement(1091..1109)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1137..1156)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     intron          1164..1736
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     promoter        complement(1745..2255)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     complement(1808..1826)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     misc_feature    2304..2421
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        complement(2522..2616)
                     /label=H1-2O2 promoter
                     /note="doxycycline-inducible variant of the human H1 RNA 
                     promoter (Henriksen et al., 2007)"
     protein_bind    2522..2540
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2550..2568
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(2586..2605)
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     CDS             complement(2905..2949)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3134..3367
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    3860..3985
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             4032..4212
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(4213..4439)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(4467..4487)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(4467..4485)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4506..4522)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4506..4522)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4519..4541)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4530..4546
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4554..4560,4561..4578,4579..4584))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4599..4620
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4737..4754)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4908..5496)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4988..5007)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5667..6458,6459..6527))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6290..6309
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6528..6632)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(6658..7113)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6795..6816)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7007..7026
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7240..7262
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7254..7271
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7255..7271
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        7281..7299
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      7320..7455
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    7495..7616
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7506..7525)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7560..7579
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             7688..7921
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     CDS             complement(8049..8648)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8132..8152)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     8629..8648
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     misc_feature    join(8668..8816,1..425)
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
ORIGIN
        1 gccccttgtt gaatacgctt gaggagagcc atttgactct ttccacaact atccaactca
       61 caacgtggca ctggggttgt gccgcctttg caggtgtatc ttatacacgt ggcttttggc
      121 cgcagaggca cctgtcgcca ggtggggggt tccgctgcct gcaaagggtc gctacagacg
      181 ttgtttgtct tcaagaagct tccagaggaa ctgcttcctt cacgacattc aacagacctt
      241 gcattccttt ggcgagaggg gaaagacccc taggaatgct cgtcaagaag acagggccag
      301 gtttccgggc cctcacattg ccaaaagacg gcaatatggt ggaaaataac atatagacaa
      361 acgcacaccg gccttattcc aagcggcttc ggccagtaac gttagggggg ggggagggag
      421 aggggcagat ccctagaact cccgggagcc gctgtacgcg gacccacttt cacatttaag
      481 ttgtttttct aatccgcata tgatcaattc aaggccgaat aagaaggctg gctctgcacc
      541 ttggtgatca aataattcga tagcttgtcg taataatggc ggcatactat cagtagtagg
      601 tgtttccctt tcttctttag cgacttgatg ctcttgatct tccaatacgc aacctaaagt
      661 aaaatgcccc acagcgctga gtgcatataa tgcattctct agtgaaaaac cttgttggca
      721 taaaaaggct aattgatttt cgagagtttc atactgtttt tctgtaggcc gtgtacctaa
      781 atgtactttt gctccatcgc gatgacttag taaagcacat ctaaaacttt tagcgttatt
      841 acgtaaaaaa tcttgccagc tttccccttc taaagggcaa aagtgagtat ggtgcctatc
      901 taacatctca atggctaagg cgtcgagcaa agcccgctta ttttttacat gccaatacaa
      961 tgtaggctgc tctacaccta gcttctgggc gagtttacgg gttgttaaac cttcgattcc
     1021 gacctcatta agcagctcta atgcgctgtt aatcacttta cttttatcta atctagacat
     1081 atcaattcgc cctatagtga gtcgtattac aattctttgc caaaatgatg agacagcaca
     1141 ataaccagca cgttgcccag gagctgtagg aaaaagaaga aggcatgaac atggttagca
     1201 gaggggcccg gtttggactc agagtatttt atcctcatct caaacagtgt atatcattgt
     1261 aaccataaag agaaaggcag gatgatgacc aggatgtagt tgtttctacc aataagaata
     1321 tttccacgcc agccagaatt tatatgcaga aatattctac cttatcattt aattataaca
     1381 attgttctct aaaactgtgc tgaagtacaa tataatatac cctgattgcc ttgaaaaaaa
     1441 agtgattaga gaaagtactt acaatctgac aaataaacaa aagtgaattt aaaaattcgt
     1501 tacaaatgca agctaaagtt taacgaaaaa gttacagaaa atgaaaagaa aataagagga
     1561 gacaatggtt gtcaacagag tagaaagtga aagaaacaaa attatcatga gggtccatgg
     1621 tgatacaagg gacatcttcc cattctaaac aacaccctga aaactttgcc ccctccatat
     1681 aacatgaatt ttacaatagc gaaaaagaaa gaacaatcaa gggtccccaa actcacagat
     1741 ccccctgggg agagaggtcg gtgattcggt caacgaggga gccgactgcc gacgtgcgct
     1801 ccggaggctt gcagaatgcg gaacaccgcg cgggcaggaa cagggcccac actaccgccc
     1861 cacaccccgc ctcccgcacc gccccttccc ggccgctgct ctcggcgcgc cctgctgagc
     1921 agccgctatt ggccacagcc catcgcggtc ggcgcgctgc cattgctccc tggcgctgtc
     1981 cgtctgcgag ggtactagtg agacgtgcgg cttccgtttg tcacgtccgg cacgccgcga
     2041 accgcaagga accttcccga cttaggggcg gagcaggaag cgtcgccggg gggcccacaa
     2101 gggtagcggc gaagatccgg gtgacgctgc gaacggacgt gaagaatgtg cgagacccag
     2161 ggtcggcgcc gctgcgtttc ccggaaccac gcccagagca gccgcgtccc tgcgcaaacc
     2221 cagggctgcc ttggaaaagg cgcaacccca accccctcga gccgcggcca aagtggatct
     2281 ctgctgtccc tgtaataaac ccgaaaattt tgaatttttg taatttgttt ttgtaattct
     2341 ttagtttgta tgtctgttgc tattatgtct actattcttt cccctgcact gtacccccca
     2401 atcccccctt ttcttttaaa attgtggatg aatactgcca tttgtctcga ggtcgagaat
     2461 tcaaaaacct ggcatgacta ccactatgct cgagcatagt ggtagtcatg ccaggccggt
     2521 gtctctatca ctgataggga acttataagt ctctatcact gatagggatt tcacgtttat
     2581 ggtgatttcc cagaacacat agcgacatgc aaatattgcg gccgctcgag gctagtctcg
     2641 tgatcgatac cgtcgagatc cgttcactaa tcgaatggat ctgtctctgt ctctctctcc
     2701 accttcttct tctattcctt cgggcctgtc gggtcccctc ggggttggga ggtgggtctg
     2761 aaacgataat ggtgaatatc cctgcctaac tctattcact atagaaagta cagcaaaaac
     2821 tattcttaaa cctaccaagc ctcctactat cattatgaat aattttatat accacagcca
     2881 atttgttatg ttaaaccaat tccacaaact tgcccattta tctaattcca ataattcttg
     2941 ttcattcttt tcttgctggt tttgcgattc ttcaattaag gagtgtatta agcttgtgta
     3001 attgttaatt tctctgtccc actccatcca ggtcgtgtga ttccaaatct gttccagaga
     3061 tttattactc caactagcat tccaaggcac agcagtggtg caaatgagtt ttccagagca
     3121 accccaaatc cccaggagct gttgatcctt taggtatctt tccacagcca ggattcttgc
     3181 ctggagctgc ttgatgcccc agactgtgag ttgcaacaga tgctgttgcg cctcaatagc
     3241 cctcagcaaa ttgttctgct gctgcactat accagacaat aattgtctgg cctgtaccgt
     3301 cagcgtcatt gacgctgcgc ccatagtgct tcctgctgct cccaagaacc caaggaacaa
     3361 agctcctatt cccactgctc ttttttctct ctgcaccact cttctctttg ccttggtggg
     3421 tgctactcct aatggttcaa tttttactac tttatattta tataattcac ttctccaatt
     3481 gtccctcata tctcctcctc caggtctgaa gatcagcggc cgcttgctgt gcggtggtct
     3541 tacttttgtt ttgctcttcc tctatcttgt ctaaagcttc cttggtgtct tttatctcta
     3601 tcctttgatg cacacaatag agggttgcta ctgtattata taatgatcta agttcttctg
     3661 atcctgtctg aagggatggt tgtagctgtc ccagtatttg tctacagcct tctgatgttt
     3721 ctaacaggcc aggattaact gcgaatcgtt ctagctccct gcttgcccat actatatgtt
     3781 ttaatttata ttttttcttt ccccctggcc ttaaccgaat tttttcccat cgcgatctaa
     3841 ttctcccccg cttaatactg acgctctcgc acccatctct ctccttctag cctccgctag
     3901 tcaaaatttt tggcgtactc accagtcgcc gcccctcgcc tcttgccgtg cgcgcttcag
     3961 caagccgagt cctgcgtcga gagagctcct ctggtttccc tttcgctttc aagtccctgt
     4021 tcgggcgcca ctgctagaga ttttccacac tgactaaaag ggtctgaggg atctctagtt
     4081 accagagtca cacaacagac gggcacacac tacttgaagc actcaaggca agctttattg
     4141 aggcttaagc agtgggttcc ctagttagcc agagagctcc caggctcaga tctggtctaa
     4201 ccagagagac ccgtttatgt atcgagctag gcacttaaat acaatatctc tgcaatgcgg
     4261 caattcagtg gttcgtccaa tccatgtcag acccgtctgt tgccttccta ataaggcacg
     4321 atcgtaccac cttacttcca ccaatcggca tgcacggtgc tttttctctc cttgtaaggc
     4381 atgttgctaa ctcatcgtta ccatgttgca agactacaag agtattgcat aagactacat
     4441 taagcttgca gctccagctt ttgttccctt tagtgagggt taattgcgcg cttggcgtaa
     4501 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     4561 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
     4621 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
     4681 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     4741 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     4801 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     4861 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     4921 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     4981 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     5041 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     5101 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     5161 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     5221 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     5281 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     5341 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     5401 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     5461 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     5521 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     5581 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     5641 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     5701 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     5761 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     5821 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     5881 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     5941 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     6001 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     6061 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     6121 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     6181 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     6241 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     6301 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     6361 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     6421 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     6481 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     6541 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     6601 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctaaa
     6661 ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt
     6721 ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag
     6781 ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg
     6841 tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat
     6901 caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc
     6961 gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga
     7021 aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac
     7081 ccgccgcgct taatgcgccg ctacagggcg cgtcccattc gccattcagg ctgcgcaact
     7141 gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat
     7201 gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa
     7261 cgacggccag tgagcgcgcg taatacgact cactataggg cgaattgggt acgtccctag
     7321 gcctccaaaa aagcctcctc actacttctg gaatagctca gaggccgagg cggcctcggc
     7381 ctctgcataa ataaaaaaaa ttagtcagcc atggggcgga gaatgggcgg aactgggcgg
     7441 agttaggggc gggatgggcg gagttagggg cgggatagct agagccagac atgataagat
     7501 acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg
     7561 aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttcctc
     7621 tcactctctg atattcattt ctttgcaagt tataaatact gaataataag atgacatgaa
     7681 ctactactgc tagagatttt ccacactgac taaaagggtc tgagggatct ctagttacca
     7741 gagtcacaca acagacgggc acacactact tgaagcactc aaggcaagct ttattgaggc
     7801 ttaagcagtg ggttccctag ttagccagag agctcccagg ctcagatctg gtctaaccag
     7861 agagacccag tacaagcaaa aagcagatct tgtcttcgtt gggagtgaat tagcccttcc
     7921 agtcccccct tttcttttaa aaagtggcta agatctacag ctgccttgta agtcattggt
     7981 cttaaaggta ccgatgcatg gggtcgtgcg ctcctttcgg tcgggcgctg cgggtcgtgg
     8041 ggcgggcgtc aggcaccggg cttgcgggtc atgcaccagg tgcgcggtcc ttcgggcacc
     8101 tcgacgtcgg cggtgacggt gaagccgagc cgctcgtaga aggggaggtt gcggggcgcg
     8161 gaggtctcca ggaaggcggg caccccggcg cgctcggccg cctccactcc ggggagcacg
     8221 acggcgctgc ccagaccctt gccctggtgg tcgggcgaga cgccgacggt ggccaggaac
     8281 cacgcgggct ccttgggccg gtgcggcgcc aggaggcctt ccatctgttg ctgcgcggcc
     8341 agccgggaac cgctcaactc ggccatgcgc gggccgatct cggcgaacac cgcccccgct
     8401 tcgacgctct ccggcgtggt ccagaccgcc accgcggcgc cgtcgtccgc gacccacacc
     8461 ttgccgatgt cgagcccgac gcgcgtgagg aagagttctt gcagctcggt gacccgctcg
     8521 atgtggcggt ccggatcgac ggtgtggcgc gtggcggggt agtcggcgaa cgcggcggcg
     8581 agggtgcgta cggccctggg gacgtcgtcg cgggtggcga ggcgcaccgt gggcttgtac
     8641 tcggtcatgg taagctccgg tggatcctta tcatcgtgtt tttcaaagga aaaccacgtc
     8701 cccgtggttc ggggggccta gacgtttttt taacctcgac taaacacatg taaagcatgt
     8761 gcaccgaggc cccagatcag atcccataca atggggtacc ttctgggcat ccttca
//
