LOCUS       sequence-274487-        9235 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9235)
  AUTHORS   Cantor S, Drapkin R, Zhang F, Lin Y, Han J, Pamidi S, Livingston DM
  TITLE     The BRCA1-associated protein BACH1 is a DNA helicase targeted by 
            clinically relevant inactivating mutations.
  JOURNAL   Proc Natl Acad Sci U S A. 2004 Feb 24. 101(8):2357-62.
  PUBMED    14983014
REFERENCE   2  (bases 1 to 9235)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2004 Feb 24. 101(8):2357-62."
COMMENT     Sequence Label: pcDNA3-myc-his-BACH1 WT
FEATURES             Location/Qualifiers
     source          1..9235
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        100..479
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        480..683
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     634..654
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     728..747
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        728..746
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             4604..4633
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             4649..4666
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(4689..4706)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4695..4919
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4965..5393
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5052..5071)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5262..5283
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5402..5422)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5407..5736
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5587..5722
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5649..5668
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5803..6597
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5857..5876)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6467..6486
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6773..6894
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6810..6829)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6864..6883
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6943..6959)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6943..6959)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6956..6978)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6967..6983
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6991..6997,6998..7015,7016..7021))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7036..7057
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7174..7191)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7345..7933)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7425..7444)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8104..8895,8896..8964))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8727..8746
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8965..9069)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9144..9163)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 ctacaacaag gcaaggcttg accgacaatt gcatgaagaa tctgcttagg gttaggcgtt
       61 ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta ttgactagtt
      121 attaatagta atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta
      181 cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt
      241 caataatgac gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg
      301 tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta
      361 cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga
      421 ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg
      481 tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc
      541 caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact
      601 ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt
      661 gggaggtcta tataagcaga gctctctggc taactagaga acccactgct tactggctta
      721 tcgaaattaa tacgactcac tatagggaga cccaagctgg ctagttaagc ttggtaccga
      781 gctcggatcc actagtccag tgtggtggaa ttctgcagat atccagcaca gtggcggccg
      841 cgccaccatg tcttcaatgt ggtctgaata tacaattggt ggggtgaaga tttactttcc
      901 ttataaagct tacccgtcac agcttgctat gatgaattct attctcagag gattaaacag
      961 caagcaacat tgtttgttgg agagtcccac aggaagtgga aaaagcttag ccttactttg
     1021 ttctgcttta gcatggcaac aatctcttag tgggaaacca gcagatgagg gcgtaagtga
     1081 aaaagctgaa gtacaattgt catgttgttg tgcatgccat tcaaaggatt ttacaaacaa
     1141 tgacatgaac caaggaactt cacgtcattt caactatcca agcacaccac cttctgaaag
     1201 aaatggcact tcatcaactt gtcaagactc ccctgaaaaa accactctgg ctgcaaagtt
     1261 atctgctaag aaacaggcat ccatatacag agatgaaaat gatgattttc aagtagagaa
     1321 gaaaagaatt cgacccttag aaactacaca gcagattaga aaacgtcatt gctttggaac
     1381 agaagtacac aatttggatg caaaagttga ttcaggaaag actgtaaaac tcaactctcc
     1441 actggaaaag ataaactcct tttcgccaca gaaaccccct ggccactgtt ctaggtgctg
     1501 ttgttctact aaacaaggaa acagtcaaga gtcatcgaat accattaaga aggatcatac
     1561 agggaaatcc aagataccca aaatatattt tgggacacgc acacacaagc agattgctca
     1621 gattactaga gagctccgga ggacggcata ttcaggggtt ccaatgacta ttctttccag
     1681 cagggatcat acttgtgtcc atcctgaggt agtcggtaac ttcaacagaa atgagaagtg
     1741 catggaattg ctagatggga aaaacggaaa atcctgctat ttttatcatg gagttcataa
     1801 aattagtgat cagcacacat tacagacttt ccaagggatg tgcaaagcct gggatataga
     1861 agaacttgtc agcctgggga agaaactaaa ggcctgtcca tattacacag cccgagaact
     1921 aatacaagat gctgacatca tattttgtcc ctacaactat cttctagatg cacaaataag
     1981 ggaaagtatg gatttaaatc tgaaagaaca ggttgtcatt ttagatgaag ctcataacat
     2041 cgaggactgt gctcgggaat cagcaagtta cagtgtaaca gaagttcagc ttcggtttgc
     2101 tcgggatgaa ctagatagta tggtcaacaa taatataagg aagaaagatc atgaacccct
     2161 acgagctgtg tgctgtagcc tcattaattg gttagaagca aacgctgaat atcttgtaga
     2221 aagagattat gaatcagctt gtaaaatatg gagtggaaat gaaatgctct taactttaca
     2281 caaaatgggt atcaccactg ctacttttcc cattttgcag ggacattttt ctgctgttct
     2341 tcaaaaagag gaaaaaatct caccaattta tggtaaagag gaggcaagag aagtacctgt
     2401 tattagtgca tcaactcaaa taatgcttaa aggacttttt atggtacttg actatctttt
     2461 taggcaaaat agcagatttg cagatgatta taaaattgcg attcaacaga cttactcctg
     2521 gacaaatcag attgatattt cagacaaaaa tgggttgttg gttctaccaa aaaataagaa
     2581 acgttcacga cagaaaactg cagttcatgt gctaaacttt tggtgcttaa atccagctgt
     2641 ggccttttca gatattaatg gcaaagttca gaccattgtt ttgacatctg gtacattatc
     2701 accaatgaaa tccttttcgt cagaacttgg tgttacattt actatccagc tggaggctaa
     2761 tcatatcatt aaaaattcac aggtttgggt tggtaccatt gggtcaggcc ccaagggtcg
     2821 gaatctctgt gctaccttcc agaatactga aacatttgag ttccaagatg aagtgggagc
     2881 acttttgtta tctgtgtgcc agactgtgag ccaaggaatt ttgtgtttct tgccatctta
     2941 caagttatta gaaaaattaa aagaacgttg gctctctact ggtttatggc ataatctgga
     3001 gttggtgaag acagtcattg tagaaccaca gggaggagaa aaaacaaatt ttgatgaatt
     3061 actgcaggtg tactatgacg caatcaaata caaaggagag aaagatggag ctctcctggt
     3121 agcagtttgt cgtggtaaag tgagtgaggg tctggatttc tcagatgaca atgcccgtgc
     3181 tgtcataaca ataggaattc cttttccaaa tgtgaaagat ctacaggttg aactaaaacg
     3241 acaatacaat gaccaccatt caaaattgag aggtcttcta cctggccgtc agtggtatga
     3301 aattcaagca tacagggcct taaaccaggc ccttggtaga tgtattagac acagaaatga
     3361 ttggggagct cttattctag tggatgatcg ctttaggaat aacccaagtc gctatatatc
     3421 tggactttct aaatgggtac ggcagcagat tcagcaccat tcaacctttg aaagtgcact
     3481 ggagtccttg gctgaatttt ccaaaaagca tcaaaaagtt cttaatgtat ccataaagga
     3541 cagaaccaat atacaggaca atgagtctac acttgaagtg acctctttaa agtacagtac
     3601 cccaccttat ttactggaag cagcaagtca tctatcacca gaaaattttg tggaagatga
     3661 agcaaagata tgtgtccagg aactacagtg tcctaaaatt attaccaaaa attcacctct
     3721 accaagtagc attatctcca gaaaggagaa aaatgatcca gtattcctgg aagaagcagg
     3781 gaaagcagaa aaaattgtga tttccagatc cacaagccca actttcaaca aacaaacaaa
     3841 gagagttagc tggtcaagct ttaattcttt gggacagtat tttactggta aaataccgaa
     3901 ggcaacacct gagctcgggt catcagagaa tagtgcctct agtcctcccc gtttcaaaac
     3961 agagaagatg gaaagtaaaa ctgttttgcc cttcactgat aaatgtgaat cctcaaatct
     4021 gacagtaaac acatcgtttg gatcatgccc tcaatcagaa accattattt catcattaaa
     4081 gattgatgcc acccttacta gaaaaaatca ttctgaacat ccgctctgtt ctgaagaagc
     4141 cctggatcca gacattgaat tgtctctagt aagtgaagaa gataaacagt ccacttcaaa
     4201 tagagatttt gaaacagaag cagaagatga atctatctat tttacacctg aactttacga
     4261 tcctgaagat acagatgaag aaaaaaatga cctagctgaa actgatagag gaaatagatt
     4321 ggctaacaat tcagattgca ttttagctaa agaccttttt gaaattagaa ctataaaaga
     4381 agtagattca gccagagaag tgaaagctga ggattgcata gatacaaagt tgaatggaat
     4441 tctgcatatt gaagaaagta aaattgatga cattgatggt aatgtaaaaa caacttggat
     4501 aaatgaactg gaactgggaa aaactcatga aatagaaata aagaacttta aaccatctcc
     4561 ttccaaaaat aaaggcatgt ttcctggttt taaggggccc ttcgaacaaa aactcatctc
     4621 agaagaggat ctgaatatgc ataccggtca tcatcaccat caccattgag tttaaacccg
     4681 ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt
     4741 gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat
     4801 tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag
     4861 caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc
     4921 ttctgaggcg gaaagaacca gctggggctc tagggggtat ccccacgcgc cctgtagcgg
     4981 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
     5041 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
     5101 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
     5161 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac
     5221 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
     5281 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat
     5341 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attaattctg
     5401 tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     5461 caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca
     5521 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact
     5581 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
     5641 atttttttta tttatgcaga ggccgaggcc gcctctgcct ctgagctatt ccagaagtag
     5701 tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc
     5761 attttcggat ctgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga
     5821 ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa
     5881 cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt
     5941 ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg
     6001 ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa
     6061 gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac
     6121 cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt
     6181 gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact
     6241 cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg
     6301 ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg
     6361 acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc
     6421 atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt
     6481 gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc
     6541 gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg
     6601 ggactctggg gttcgcgaaa tgaccgacca agcgacgccc aacctgccat cacgagattt
     6661 cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg
     6721 ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt
     6781 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     6841 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     6901 ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt
     6961 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
     7021 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
     7081 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
     7141 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     7201 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     7261 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     7321 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     7381 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     7441 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     7501 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     7561 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     7621 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     7681 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     7741 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     7801 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     7861 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     7921 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     7981 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     8041 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     8101 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     8161 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     8221 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     8281 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     8341 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     8401 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     8461 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     8521 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     8581 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     8641 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     8701 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     8761 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     8821 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     8881 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     8941 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     9001 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     9061 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc gacggatcgg gagatctccc
     9121 gatcccctat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat
     9181 ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat ttaag
//
