LOCUS       sequence-274507-        8876 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8876)
  AUTHORS   Munger K, Phelps WC, Bubb V, Howley PM, Schlegel R
  TITLE     The E6 and E7 genes of the human papillomavirus type 16 together are
            necessary and sufficient for transformation of primary human 
            keratinocytes.
  JOURNAL   J Virol. 1989 Oct . 63(10):4417-21.
  PUBMED    2476573
REFERENCE   2  (bases 1 to 8876)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            1989 Oct . 63(10):4417-21."
COMMENT     Sequence Label: pB-actin E6 E7
FEATURES             Location/Qualifiers
     source          1..8876
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(3897..3914)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4068..4656)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4148..4167)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4827..5618,5619..5687))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5450..5469
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5688..5792)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5860..5878
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ctgcaggagc gtacagaacc cagggccctg gcacccgtgc agaccctggc ccaccccacc
       61 tgggcgctca gtgcccaaga gatgtccaca cctaggatgt cccgcggtgg gtggggggcc
      121 cgagagacgg gcaggccggg ggcaggcctg gccatgcggg gccgaaccgg gcactgccca
      181 gcgtggggcg cgggggccac ggcgcgcgcc cccagccccc gggcccagca ccccaaggcg
      241 gccaacgcca aaactctccc tcctcctctt cctcaatctc gctctcgctc tttttttttt
      301 tcgcaaaagg aggggagagg gggtaaaaaa atgctgcact gtgcggcgaa gccggtgagt
      361 gagcggcgcg gggccaatca gcgtgcgccg ttccgaaagt tgccttttat ggctcgagcg
      421 gccgcggcgg cgccctataa aacccagcgg cgcgacgcgc caccaccgcc gagaccgcgt
      481 ccgccccgcg agcacagagc ctcgcctttg ccgatccgcc gcccgtccac acccgccgcc
      541 aggtaagccc ggccagccga ccggggcatg cggccgcggc cccttcgccc gtgcagagcc
      601 gccgtctggg ccgcagcggg gggcgcatgg ggggggaacc ggaccgccgt ggggggcgcg
      661 ggagaagccc ctgggcctcc ggagatgggg gacaccccac gccagttcgg aggcgcgagg
      721 ccgcgctcgg gaggcgcgct ccgggggtgc cgctctcggg gcgggggcaa ccggcggggt
      781 ctttgtctga gccgggctct tgccaatggg gatcgcaggg tgggcgcggc gtagcccccg
      841 ccaggcccgg tgggggctgg ggcgccattg cgcgtgcgcg ctggtccttt gggcgctaac
      901 tgcgtgcgcg ctgggaattg gcgctaattg cgcgtgcgcg ctgggactca aggcgctaat
      961 tgcgcgtgcg ttctggggcc cggggtgccg cggcctgggc tggggcgaag gcgggctcgg
     1021 ccggaagggg tggggtcgcc gcggctcccg ggcgcttgcg cgcacttcct gcccgagccg
     1081 ctggccgccc gagggtgtgg ccgctgcgtg cgcgcgcgcc gacccggcgc tgtttgaacc
     1141 gggcggaggc ggggctggcg cccggttggg agggggttgg ggcctggctt cctgccgcgc
     1201 gccgcgggga cgcctccgac cagtgtttgc cttttatggt aataacgcgg ccggcccggc
     1261 ttcctttgtc cccaatctgg gcgcgcgccg gcgccccctg gcggcctaag gactcggcgc
     1321 gccggaagtg gccagggcgg gggcgacttc ggctcacagc gcgcccggct attctcgcag
     1381 gatcagtcga ctctagagga tccttttatg caccaaaaga gaactgcaat gtttcaggac
     1441 ccacaggagc gacccagaaa gttaccacag ttatgcacag agctgcaaac aactatacat
     1501 gatataatat tagaatgtgt gtactgcaag caacagttac tgcgacgtga ggtatatgac
     1561 tttgcttttc gggatttatg catagtatat agagatggga atccatatgc tgtatgtgat
     1621 aaatgtttaa agttttattc taaaattagt gagtatagac attattgtta tagtttgtat
     1681 ggaacaacat tagaacagca atacaacaaa ccgttgtgtg atttgttaat taggtgtatt
     1741 aactgtcaaa agccactgtg tcctgaagaa aagcaaagac atctggacaa aaagcaaaga
     1801 ttccataata taaggggtcg gtggaccggt cgatgtatgt cttgttgcag atcatcaaga
     1861 acacgtagag aaacccagct gtaatcatgc atggagatac acctacattg catgaatata
     1921 tgttagattt gcaaccagag acaactgatc tctactgtta tgagcaatta aatgacagct
     1981 cagaggagga ggatgaaata gatggtccag ctggacaagc agaaccggac agagcccatt
     2041 acaatattgt aaccttttgt tgcaagtgtg actctacgct tcggttgtgc gtacaaagca
     2101 cacacgtaga cattcgtact ttggaagacc tgttaatggg cacactagga attgtgtgcc
     2161 ccatctgttc tcagaaacca taatctacca tggctgatcc tgcaggtacc ggatccggta
     2221 ccgagctcga attcaagctt ggaattctca tgtttgacag cttatcatcg ataagctact
     2281 caggatttaa aaactggaac ggtgaaggtg acagcagtcg gttggagcga gcatccccca
     2341 aagttcacaa tgtggccgag gactttgatt gcacattgtt gtttttttaa tagtcattcc
     2401 aaatatgaga tgcattgtta caggaagtcc cttgccatcc taaaagccac cccacttctc
     2461 tctaaggaga atggcccagt cctctcccaa gtccacacag gggaggtgat agcattgctt
     2521 tcgtgtaaat tatgtaatgc aaaatttttt taatcttcgc cttaatactt ttttattttg
     2581 ttttattttg aatgatgagc cttcgtgccc ccccttcccc cttttttgtc ccccaacttg
     2641 agatgtatga aggcttttgg tctccctggg agtgggtgga ggcagccagg gcttacctgt
     2701 acactgactt gagaccagtt gaataaaagt gcacacctta aaaatgaggc caagtgtgac
     2761 tttgtggtgt ggctgggttg ggggcagcag agggtgaacc ctgcaggagg gtgaaccctg
     2821 caaaagggtg gggcagtggg ggccaacttg tcctcaccca gagtgcaggt gtgtggagat
     2881 ccctcctgcc ttgacattga gcagccttag agggtggggg aggctcaggg gtcaggtctc
     2941 tgttcctgct tattggggag ttcctggcct ggcccttcta tgtctcccca ggtaccccag
     3001 tttttctggg ttcacccaga gtgcagatgc ttgaggaggt gggaagggac tatttggggg
     3061 tgtctggctc aggtgccatg cctcactggg gctggttggc acctgcattt cctgggagtg
     3121 gggctgtctc agggtagctg ggcacggtgt tcccttgagt gggggtgtag tgagtgttcc
     3181 tagctgccac gcctttgcct tcacctatgg gatcgtggct gtcagccttg agggtcagcc
     3241 tggcccaggc tcccataggc ttaggagagg ccgcaattcc tacctgttca tccagacaga
     3301 gggggacctg gaatcaaagt caagttgggg tggggggtcc atggggccat atctggcctg
     3361 cagacagctc tggttagcta tgggctgagg tctggattct gccttgtgac tggagactgg
     3421 gcgccatccc gtggcctctg agggcccagt ttgttcgtga cccatgctgc agagggagat
     3481 acccaggctc ctggacctca gggcctgcct ggcactgcag gccaggttct ctttttattt
     3541 ctgagacggg gtcttgtgtt gcccaggctg gagtgtaatg gcacaaccat ggctcactgc
     3601 agcctcaact ttgggctcaa gcaattctcc tgcctcagcc tcccaagtag ctgaaactac
     3661 acaggcatgc cccagcacgc ccagctagtt gcaggtcaga ttctcattgt tctacagggc
     3721 cctgagttaa atactctcgc aagaagaaac caagcctggc ctgccttccc tctgctagaa
     3781 gctgtgattg acaccagcag agaatgaagg aagaaaaggg ggtgaggatc tatgcggtgt
     3841 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg
     3901 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     3961 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     4021 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     4081 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     4141 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     4201 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     4261 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     4321 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     4381 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     4441 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     4501 actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     4561 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     4621 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     4681 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     4741 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     4801 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     4861 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     4921 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     4981 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     5041 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     5101 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctgcaggcat cgtggtgtca
     5161 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     5221 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     5281 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     5341 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     5401 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caacacggga taataccgcg
     5461 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     5521 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     5581 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     5641 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     5701 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     5761 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     5821 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
     5881 tttcttatca tcgaattccg actttcaaac agcctttgga ctcaggcctt ggggacattc
     5941 caggggaccc tttaccaggg aggggcagtg tgaggcagga ggcagggctg ccgcctcagg
     6001 gaccctgagc ctagagtcct tcccaccgca tttggggact tgagcacttc accagtcctg
     6061 actcagtttc ctagtctgtg aatgggggtg tgtctcctgg aatgttgccc cccctcattc
     6121 attcattctc ctctcactga gcacctaccg tgttcagggt ccctgtcctc ctagagctaa
     6181 ctcaagtctt ggagggaagc tctgtctcag ttccttcctc tacaaaatgg agcttgagga
     6241 cgggcacggt ggctcatgcc tgtaatccca ggactttggg aggctgaggt gggaggatcg
     6301 cttgagcccc ccggagctgg agaccagcct gggcaacatg gtgagaagcc atacctctac
     6361 agataattta aaaattagcc aggcttggaa gtgtgtgcct atagtcccag ctactcgtga
     6421 aggctttgag cccaggaggt cgaggctgca gtgagctgtg atcgcactcc agcctgggca
     6481 tcacagcaag accttgtatc aaaaatagta ataataataa aaaggaggtt ggattccctc
     6541 ctggcaggat agggagggcg ctgcagtgcc cagggcaagg tggctgggtg gttgttttgc
     6601 gggagggcca aggagtggtc cctgggtctg cgctgtaaga gttggttgcc tggaggcctt
     6661 gcagggtggg ggtgtcacca aagcaaaggc ttggagggga aaaaacaaaa gtcccagcca
     6721 ggcaggtgga gtccccttgg ctgagttcaa ccgagggttc tccgggggct gcgtgcgtgc
     6781 cccagtgaca gctccgaaag ctcccttaca gggcaaagtt cccaagcaca gaagagaacc
     6841 tgttcacttc tcccctgctc ggcccgcccc ctggccaggc acctctactt cctcttttcc
     6901 tgctccgctg cttgctttct ctcttcagct cctccctgcc cctcacccca ggctgctcgg
     6961 ccacctccaa cctgccacct gaggacaccc aggcagtcac tcattcaaca gcgaggagcc
     7021 ctggggtggg tgtagtggga aggagtgggg gtgacggaga ccctgggagg gctcgcagcc
     7081 tggtggctga ggcccagttc taaatgccag ctgcaagcct tggtctgagg tagggaggaa
     7141 ggcgtggctg cagaggctaa aacgcttccc caaagagggg ctttctggga tgggacttga
     7201 agggtgcata ggagagcact aggaagtggc cgctgcagac agagggaacc acaagccagg
     7261 aggacaggcc aggaatgctg cagcccgggg cggggtgggg ctggagctcc tgtctcttgg
     7321 ccagctgaat ggaggcccag cggcaacaca ggtcctgcct ggggatcagg tctgctctgc
     7381 accccacctt gctgcctgga gccgcccacc tgacaacctc tcatccctgc tctgtagatc
     7441 cggtcccatc cccactgccc accccacccc cccagcactc cacccagttc aacgttccac
     7501 gaacccccag aaccagccct catcaacagg cagcaagaag ggccccccgc ccatcgcccc
     7561 acaacgccag ccgggtgaac tgtagcgttg gcaggtcctg aggcagctgg caagacgcct
     7621 gcagctgaaa gatacaaggc cagggacagg acagtcccat ccccaggagg cagggagtat
     7681 acaggctggg gaagtttgcc cttgcgtggg gtggtgatgg aggaggctca gcaagtcttc
     7741 tggactgtga acctgtgtct gccactgtgt gctgggtggt ggtcatcttt cccaccaggc
     7801 tgtggcctct gcaaccttca agggaggagc aggtcccatt ggctgagcac agccttgtac
     7861 cgtgaactgg aacaagcagc ctccttcctg gccacaggtt ccatgtcctt atatggactc
     7921 atctttgcct attgcgacac acactcaatg aacacctact acgcgctgca aagagccccg
     7981 caggcctgag gtgcccccac ctcaccactc ttcctatttt tgtgtaaaaa tccagcttct
     8041 tgtcaccacc tccaaggagg gggaggagga ggaaggcagg ttcctctagg ctgagccgaa
     8101 tgcccctctg tggtcccacg ccactgatcg ctgcatgccc accacctggg tacacacagt
     8161 ctgtgattcc cggagcagaa cggaccctgc ccacccggtc ttgtgtgcta ctcagtggac
     8221 agacccaagg caagaaaggg tgacaaggac agggtcttcc caggctggct ttgagttcct
     8281 agcaccgccc cgcccccaat cctctgtggc acatggagtc ttggtcccca gagtccccca
     8341 gcggcctcca gatggtctgg gagggcagtt cagctgtggc tgcgcatagc agacatacaa
     8401 cggacggtgg gcccagaccc aggctgtgta gacccagccc ccccgccccg cagtgcctag
     8461 gtcacccact aacgccccag gcctggtctt ggctgggcgt gactgttacc ctcaaaagca
     8521 ggcagctcca gggtaaaagg tgccctgccc tgtagagccc accttccttc ccagggctgc
     8581 ggctgggtag gtttgtagcc ttcatcacgg gccacctcca gccactggac cgctggcccc
     8641 tgccctgtcc tggggagtgt ggtcctgcga cttctaagtg gccgcaagcc acctgactcc
     8701 cccaacacca cactctacct ctcaagccca ggtctctccc tagtgaccca cccagcacat
     8761 ttagctagct gagccccaca gccagaggtc ctcaggccct gctttcaggg cagttgctct
     8821 gaagtcggca agggggagtg actgcctggc cactccatgc cctccaagag ctcctt
//
