LOCUS       sequence-274529-        1032 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  BglFusion compatible integration vector for Streptococcus 
            pneumoniae.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1032)
  AUTHORS   Keller LE, Rueff AS, Kurushima J, Veening JW
  TITLE     Three New Integration Vectors and Fluorescent Proteins for Use in 
            the Opportunistic Human Pathogen Streptococcus pneumoniae.
  JOURNAL   Genes (Basel). 2019 May 22;10(5). pii: genes10050394. doi: 
            10.3390/genes10050394.
  PUBMED    31121970
REFERENCE   2  (bases 1 to 1032)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes 
            (Basel)."; date: "2019-05-22"; volume: "10(5). pii: genes10050394. 
            doi"; pages: " 10.3390/genes10050394"
COMMENT     Sequence Label: pPEPY
FEATURES             Location/Qualifiers
     source          1..1032
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(957..984)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
ORIGIN
        1 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
       61 gccagcaacg cggccttttt acggttcccg gccttttgct ggccttttgc tcacatgttc
      121 tttcctgcgt tatcccgcgg ccgcataaaa acattcatca taacccccag aacttaaata
      181 acaattttta ttcaagatac atactcctag aataaacttt atatgaaatt ctcatttttg
      241 tttttacaat tctccttagt taaatcttgt ttaatatatg ttttacatat agtatttagc
      301 gccacatagt actgaactct ctccaaaaac ggttattcct ctttgaatag ggcgttatca
      361 caagaaaagt atctccacgt ttcaacttca tatggctcaa aaacaatcaa ttgatgctaa
      421 aacctgtacc tagatgtttc ggttcataaa accatgaaac tgtaaaagtg gatgaaattg
      481 atagcgatag tcaaatcaag aggcatcata actctaaaaa gtcacaatat ataagttcat
      541 cctcggaaaa atatcattct aattgttgaa atgcctacat gaaaagaaac gtcaaatgct
      601 catgaaacaa cgaatacagg tatcaaaact atgacaaaac aagtccctaa atttactaaa
      661 gacactgctc aactgcgcta tcaaaagaat cttgcttggc aaggttcttt tttttgtctt
      721 ttcaaaaaag cacgaaaaaa gcacccataa ggtaaacttt tgagtgcttt gctataaata
      781 cgcttcacag tttcttcttc atttttaaca aacaaaaaac ccagctcatt gagctgggtt
      841 taagctggct tggcggcgtc ctagtactgc agaattgctt acactttctc gagttactgg
      901 agggatccgg cattaatttt cctccttatt tatttagatc tcatgaattc tataaacgca
      961 gaaaggccca cccgaaggtg agccagtgtg actctagtag agagcattca ccgacaaaca
     1021 acagataaaa cg
//
