LOCUS       sequence-274535-       12268 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor plasmid for SNCA exon2 wild type sequence. Also contains 
            TagBFP and EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12268)
  AUTHORS   Arias-Fuenzalida J, Jarazo J, Qing X, Walter J, Gomez-Giro G, 
            Nickels SL, Zaehres H, Scholer HR, Schwamborn JC
  TITLE     FACS-Assisted CRISPR-Cas9 Genome Editing Facilitates Parkinson's 
            Disease Modeling.
  JOURNAL   Stem Cell Reports. 2017 Nov 14;9(5):1423-1431. doi: 
            10.1016/j.stemcr.2017.08.026. Epub 2017 Oct 5.
  PUBMED    28988985
REFERENCE   2  (bases 1 to 12268)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stemcr.2017.08.026"; journalName: "Stem Cell Reports"; 
            date: "2017-11-14- 14"; volume: "9"; issue: "5"; pages: "1423-1431"
COMMENT     Sequence Label: pDONOR-SNCAe2-WT
FEATURES             Location/Qualifiers
     source          1..12268
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        75..378
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          659..1675
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     CDS             1734..2435
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     polyA_signal    2579..2634
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     repeat_region   4221..4255
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     enhancer        4601..4904
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          5185..6201
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     regulatory      6254..6263
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(6260..6262,6263..6265,6266..6976)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(6260..6262,6263..6265,6266..6976)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6305..6326)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6566..6585)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6913..6934
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             6977..7030
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             7031..7627
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="TEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERV
                     TELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAA
                     QQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSA
                     PRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     7524..7544
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    7771..7826
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     repeat_region   8356..8418
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(9416..9436)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(9416..9434)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(9455..9471)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9455..9471)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9468..9490)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9479..9495
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9503..9509,9510..9527,9528..9533))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9548..9569
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9686..9703)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9857..10445)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9937..9956)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10616..11407,11408..11476))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11239..11258
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11477..11581)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(11607..12062)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11744..11765)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     11956..11975
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     12189..12211
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     12203..12220
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     12204..12220
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12230..12249
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        12230..12248
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 cattgattat tgactagtta ctaatagtaa tcaattacgg ggtcattagt tcatagccca
       61 tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac
      121 gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact
      181 ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa
      241 gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg
      301 cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta
      361 gtcatcgcta ttaccatggt cgaggtgagc cccacgttct gcttcactct ccccatctcc
      421 cccccctccc cacccccaat tttgtattta tttatttttt aattattttg tgcagcgatg
      481 ggggcggggg gggggggggg ggcgcgcgcs rggsggggsg gggsggggsg gggggsgggg
      541 sggggsgrgg sggagaggtg cggcggcagc caatcagagc ggcgcgctcc gaaagtttcc
      601 ttttatggcg aggcggcggc ggcggcggcc ctataaaaag cgaagcgcgc ggcgggcggg
      661 agtcgctgcg cgctgccttc gccccgtgcc ccgctccgcc gccgcctcgc gccgcccgcc
      721 ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc
      781 gggctgtaat tagcgcttgg tttaatgacg gcttgtttct tttctgtggc tgcgtgaaag
      841 ccttgagggg ctccgggagg gccctttgtg cggggggagc ggctcggggg gtgcgtgcgt
      901 gtgtgtgtgc gtggggagcg ccgcgtgcgg ctccgcgctg cccggcggct gtgagcgctg
      961 cgggcgcggc gcggggcttt gtgcgctccg cagtgtgcgc gaggggagcg cggccggggg
     1021 cggtgccccg cggtgcgggg ggggctgcga ggggaacaaa ggctgcgtgc ggggtgtgtg
     1081 cgtggggggg tgagcagggg gtgtgggcgc gtcggtcggg ctgcaacccc ccctgcaccc
     1141 ccctccccga gttgctgagc acggcccggc ttcgggtgcg gggctccgta cggggcgtgg
     1201 cgcggggctc gccgtgccgg gcggggggtg gcggcaggtg ggggtgccgg gcggggcggg
     1261 gccgcctcgg gccggggrgg gctcgggggr ggggcgcggc ggcccccgga gcgccggcgg
     1321 ctgtcgaggc gcggcgagcc gcagccattg ccttttatgg taatcgtgcg agagggcgca
     1381 gggacttcct ttgtcccaaa tctgtgcgga gccgaaatct gggaggcgcc gccgcacccc
     1441 ctctagcggg cgcggggcga agcggtgcgg cgccggcagg aaggaaatgg gcggggaggg
     1501 ccttcgtgcg tcgccgcgcc gccgtcccct tctccctctc cagcctcggg gctgtccgcg
     1561 gggggacggc tgccttcggg ggggacgggg cagggcgggg ttcggcttct ggcgtgtgac
     1621 cggcggctct agagcctctg ctaaccatgt tcatgccttc ttctttttcc tacagctcct
     1681 gggcaacgtg ctggttattg tgctgtctca tcattttggc aaaggccgcc accatgagcg
     1741 agctgattaa ggagaacatg cacatgaagc tgtacatgga gggcaccgtg gacaaccatc
     1801 acttcaagtg cacatccgag ggcgaaggca agccctacga gggcacccag accatgagaa
     1861 tcaaggtggt cgagggcggc cctctcccct tcgccttcga catcctggct actagcttcc
     1921 tctacggcag caagaccttc atcaaccaca cccagggcat ccccgacttc ttcaagcagt
     1981 ccttccctga gggcttcaca tgggagagag tcaccacata cgaagacggg ggcgtgctga
     2041 ccgctaccca ggacaccagc ctccaggacg gctgcctcat ctacaacgtc aagatcagag
     2101 gggtgaactt cacatccaac ggccctgtga tgcagaagaa aacactcggc tgggaggcct
     2161 tcaccgagac gctgtacccc gctgacggcg gcctggaagg cagaaacgac atggccctga
     2221 agctcgtggg cgggagccat ctgatcgcaa acatcaagac cacatataga tccaagaaac
     2281 ccgctaagaa cctcaagatg cctggcgtct actatgtgga ctacagactg gaaagaatca
     2341 aggaggccaa caacgagacc tacgtcgagc agcacgaggt ggcagtggcc agatactgcg
     2401 acctccctag caaactgggg cacaagctta attaacactc ctcaggtgca ggctgcctat
     2461 cagaaggtgg tggctggtgt ggccaatgcc ctggctcaca aataccactg agatcttttt
     2521 ccctctgcca aaaattatgg ggacatcatg aagccccttg agcatctgac ttctggctaa
     2581 taaaggaaat ttattttcat tgcaatagtg tgttggaatt ttttgtgtct ctcactcgga
     2641 aggacatatg ggagggcaaa tcatttaaaa catcagaatg agtatttggt ttagagtttg
     2701 gcaacatatg cccatatgct ggctgccatg aacaaaggtt ggctataaag aggtcatcag
     2761 tatatgaaac agccccctgc tgtccattcc ttattccata gaaaagcctt gacttgaggt
     2821 tagatttttt ttatattttg ttttgtgtta tttttttctt taacatccct aaaattttcc
     2881 ttacatgttt tactagccag atttttcctc ctctcctgac tactcccagt catagctgtc
     2941 cctcttctct tatggagatc cctcgacctg cagcccaagc ttggatcccc taggtttgtg
     3001 gatctaaacg ggcgtctttg gaaatcctgg agaacgccgg gtgggagacg aatggtcgtg
     3061 ggcaccggga gggggtggtg ctgccatgag gacccgctgg gccaggtctc tgggaggtga
     3121 gtacttgtcc ctttggggag cctaaggaaa gagacttgac ctggctttcg tcctgcttct
     3181 gatattccct tctccacaag ggctgagaga ttaggctgct tctccgggat ccgcttttcc
     3241 ccgggaaacg cgaggatgct ccatggagcg tgagcatcca acttttctct cacataaaat
     3301 ctgtctgccc gctctcttgg tttttctctg taaagtaagc aagctgcgtt tggcaaataa
     3361 tgaaatggaa gtgcagggag gccaagtcaa caggtggtaa cgggttaaca agtgctggcg
     3421 cggggtccgc tagggtggag gctgagaacg ccccctcggg tggctggcgc ggggttggag
     3481 acggccggcg agtgtgagcg gcgcctgctc agggtagata gctgagggcg ggggtggatg
     3541 ttggatggat tagaaccatc acacttgggc ccgctgtttg cctgaggttg aaccacaccc
     3601 cgagtgagta gttagttctg ttgcctacgc ctttccacca tcaacctgtt agccttcttc
     3661 tgggattcat gttaaggata cccctgaccc taagcctcca gcttccatgc ttctaactca
     3721 tactgttacc ctttagaccc cgggaattta aaaaaggggt taatcttttc atgcaactcc
     3781 acttctgaaa tgcagtaata acaactcaga ggattcatcc taatccgtgg ttaggtggct
     3841 agacttttac tagccaagat ggatgggaga tgctaaattt ttaatgccag agctaaaaat
     3901 gtctgctttg tccaatggtt aaatgagtgt acacttaaaa gagtctcaca ctttggaggg
     3961 tttctcatga tttttcagtg ttttttgttt atttttcccc gaaagttctc attcaaagtg
     4021 tattttatgt tttccagtgt ggtgtaaagg aattcattag ccatggatgt attcatgaaa
     4081 ggactttcaa aggccaagga gggagttgtg gctgctgctg agaaaaccaa acagggtgtg
     4141 gcagaagcag caggaaagac aaaagagggt gttctctatg taggtaggta aaccccaaat
     4201 gtcagtttgg tgcttgttaa ccctagaaag atagtctgcg taaaattgac gcatgcattc
     4261 ttgaaatatt gctctctctt tctaaatagc gcgaatccgt cgctgtgcat ttaggacatc
     4321 tcagtcgccg cttggagctc ccgtgaggcg tgcttgtcaa tgcggtaagt gtcactgatt
     4381 ttgaactata acgaccgcgt gagtcaaaat gacgcatgat tatcttttac gtgactttta
     4441 agatttaact catacgataa ttatattgtt atttcatgtt ctacttacgt gataacttat
     4501 tatatatata ttttcttgtt atagatatca actagaatgc tagttattaa tagtaatcaa
     4561 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa
     4621 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     4681 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     4741 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     4801 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     4861 ctacttggca gtacatctac gtattagtca tcgctattac catggtcgag gtgagcccca
     4921 cgttctgctt cactctcccc atctcccccc cctccccacc cccaattttg tatttattta
     4981 ttttttaatt attttgtgca gcgatggggg cggggggggg ggggggggcg cgcgcsrggs
     5041 ggggsggggs ggggsgrggg gsggggsggg gsgrggsggr grggtgcggc ggcagccaat
     5101 cagagcggcg cgctccgaaa gtttcctttt atggcgaggc ggcggcggcg gcggccctat
     5161 aaaaagcgaa gcgcgcggcg ggcgggagtc gctgcgcgct gccttcgccc cgtgccccgc
     5221 tccgccgccg cctcgcgccg cccgccccgg ctctgactga ccgcgttact cccacaggtg
     5281 agcgggcggg acggcccttc tcctccgggc tgtaattagc gcttggttta atgacggctt
     5341 gtttcttttc tgtggctgcg tgaaagcctt gaggggctcc gggagggccc tttgtgcggg
     5401 gggagcggct cggggggtgc gtgcgtgtgt gtgtgcgtgg ggagcgccgc gtgcggctcc
     5461 gcgctgcccg gcggctgtga gcgctgcggg cgcggcgcgg ggctttgtgc gctccgcagt
     5521 gtgcgcgagg ggagcgcggc cgggggcggt gccccgcggt gcgggggggg ctgcgagggg
     5581 aacaaaggct gcgtgcgggg tgtgtgcgtg ggggggtgag cagggggtgt gggcgcgtcg
     5641 gtcgggctgc aaccccccct gcacccccct ccccgagttg ctgagcacgg cccggcttcg
     5701 ggtgcggggc tccgtacggg gcgtggcgcg gggctcgccg tgccgggcgg ggggtggcgg
     5761 caggtggggg tgccgggcgg ggcggggccg cctcgggccg gggrgggctc gggggrgggg
     5821 cgcggcggcc cccggagcgc cggcggctgt cgaggcgcgg cgagccgcag ccattgcctt
     5881 ttatggtaat cgtgcgagag ggcgcaggga cttcctttgt cccaaatctg tgcggagccg
     5941 aaatctggga ggcgccgccg caccccctct agcgggcgcg gggcgaagcg gtgcggcgcc
     6001 ggcaggaagg aaatgggcgg ggagggcctt cgtgcgtcgc cgcgccgccg tccccttctc
     6061 cctctccagc ctcggggctg tccgcggggg gacggctgcc ttcggggggg acggggcagg
     6121 gcggggttcg gcttctggcg tgtgaccggc ggctctagag cctctgctaa ccatgttcat
     6181 gccttcttct ttttcctaca gctcctgggc aacgtgctgg ttattgtgct gtctcatcat
     6241 tttggcaaag gccgccacca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc
     6301 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg
     6361 cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct
     6421 gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg
     6481 ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt
     6541 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa
     6601 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga
     6661 cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg tctatatcat
     6721 ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga
     6781 cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt
     6841 gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga
     6901 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat
     6961 ggacgagctg tacaaggagg gcagaggaag tcttctaaca tgcggtgacg tggaggagaa
     7021 tcccggccct accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtcccccg
     7081 ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga
     7141 cccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg
     7201 gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac
     7261 gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt
     7321 gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc
     7381 caaggagccc gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg
     7441 tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc
     7501 cttcctggag acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt
     7561 caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg
     7621 tgcctgacac tcctcaggtg caggctgcct atcagaaggt ggtggctggt gtggccaatg
     7681 ccctggctca caaataccac tgagatcttt ttccctctgc caaaaattat ggggacatca
     7741 tgaagcccct tgagcatctg acttctggct aataaaggaa atttattttc attgcaatag
     7801 tgtgttggaa ttttttgtgt ctctcactcg gaaggacata tgggagggca aatcatttaa
     7861 aacatcagaa tgagtatttg gtttagagtt tggcaacata tgcccatatg ctggctgcca
     7921 tgaacaaagg ttggctataa agaggtcatc agtatatgaa acagccccct gctgtccatt
     7981 ccttattcca tagaaaagcc ttgacttgag gttagatttt ttttatattt tgttttgtgt
     8041 tatttttttc tttaacatcc ctaaaatttt ccttacatgt tttactagcc agatttttcc
     8101 tcctctcctg actactccca gtcatagctg tccctcttct cttatggaga tccctcgacc
     8161 tgcagcccaa gcttggatcc ctcgactttt gttactttat agaagaaatt ttgagttttt
     8221 gttttttttt aataaataaa taaacataaa taaattgttt gttgaattta ttattagtat
     8281 gtaagtgtaa atataataaa acttaatatc tattcaaatt aataaataaa cctcgatata
     8341 cagaccgata aaacacatgc gtcaatttta cgcatgatta tctttaacgt acgtcacaat
     8401 atgattatct ttctagggtt aatgagtgat gcgttaggat aatcaatact ctaaatgctg
     8461 gtagttctct ctcttgattc atttttgcat cattgcttgt caaaaaggtg gactgagtca
     8521 gaggtatgtg tacgtaggtg aatgtgaacg tgtgtatttg agctaatagt aaaaaatgcg
     8581 actgtttgct tttccagatt tttaattttg ccctaatatt tatgactttt taaaaataaa
     8641 tgtttctgta cctacataat tctatttcag agaacagttt taaaaactca tagtctttta
     8701 aaaaataatc aagaatattc ttaagaatca aaatcattga tggatctgtg atttctttta
     8761 ccatcatgaa aaatgtttgt caattttaat ccattctgat ttttaaaata tgactttgat
     8821 atgcccctgt gatgtgtata aagagaccta tttgtggccc taaaatggaa agaacagatt
     8881 agtctttgat agagttactt catgtgatca tttggtctct gtgaacactg aggacagaga
     8941 aaagtgcttg agggctgcta ctaatctctc agaaacattt gtatagttca tccatcaaat
     9001 gacacacata ctaaaagaat aaagaaattg atgcttatta cctacttgtt cctaaagttc
     9061 caccttgggg tatacaccca aactctgact ctcttttctg taacttgaac tgtattcaat
     9121 tgagtgttat tttacaaacc actttgaatt ccttggaaaa gaatagacac acattctcat
     9181 ccacaggcat agacacacac actcaacaca gacacattgc ccattcttcc tctcttcttt
     9241 ctcctctgag ctttttcaca ttctctggtg gcaactatag cagtaagagt cacaggatga
     9301 acagtcaggt ggaggatgac cacattgagt tgcctagctg aaacatgtgc tccgtctatg
     9361 tctgcaaaac ctagtatacg cgtatgcggc cgcttaatta atccagcttt tgttcccttt
     9421 agtgagggtt aattgcgcgc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
     9481 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     9541 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     9601 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     9661 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     9721 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     9781 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     9841 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     9901 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     9961 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
    10021 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
    10081 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
    10141 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
    10201 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
    10261 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
    10321 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
    10381 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
    10441 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
    10501 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
    10561 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
    10621 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
    10681 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
    10741 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
    10801 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
    10861 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
    10921 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
    10981 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
    11041 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
    11101 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
    11161 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
    11221 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
    11281 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
    11341 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
    11401 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
    11461 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
    11521 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
    11581 gcacatttcc ccgaaaagtg ccacctaaat tgtaagcgtt aatattttgt taaaattcgc
    11641 gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc
    11701 ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag
    11761 tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga
    11821 tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc
    11881 actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa
    11941 cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt
    12001 agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc
    12061 gtcccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc
    12121 gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc
    12181 agggttttcc cagtcacgac gttgtaaaac gacggccagt gagcgcgcgt aatacgactc
    12241 actatagggc gaattggggc gcgccatt
//
