LOCUS       sequence-274547-       11190 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of Leishmania donovani ectodomain in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11190)
  AUTHORS   Roberts AJ, Ong HB, Clare S, Brandt C, Harcourt K, Franssen SU, 
            Cotton JA, Muller-Sienerth N, Wright GJ
  TITLE     Systematic identification of genes encoding cell surface and 
            secreted proteins that are essential for in vitro growth and 
            infection in Leishmania donovani.
  JOURNAL   PLoS Pathog. 2022 Feb 24;18(2):e1010364. doi: 
            10.1371/journal.ppat.1010364. eCollection 2022 Feb.
  PUBMED    35202447
REFERENCE   2  (bases 1 to 11190)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Pathog."; date: "2022-02-24"; volume: "18(2):e1010364. doi"; pages: 
            " 10.1371/journal.ppat.1010364. eCollection 2022 Feb"
COMMENT     Sequence Label: D19_Ld_130940
FEATURES             Location/Qualifiers
     source          1..11190
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     201..225
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     706..725
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      748..757
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5968..5985
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    6037..6092
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(6091..6110)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(8328..8346)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8414..8518
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8519..8587,8588..9379)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8737..8756)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9550..10138
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10039..10058
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10292..10309
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10426..10447
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10462..10467,10468..10485,10486..10492)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10500..10516
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10505..10527
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10524..10540
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10524..10540
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     enhancer        10811..11190
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta caccaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcctcac tctcttccgc
      241 atcgctgtct gcgagggcca gctgttgggc tcgcggttga ggacaaactc ttcgcggtct
      301 ttccagtact cttggatcgg aaacccgtcg gcctccgaac ggtactccgc caccgaggga
      361 cctgagcgag tccgcatcga ccggatcgga aaacctctcg agaaaggcgt ctaaccagtc
      421 acagtcgcaa ggtaggctga gcaccgtggc gggcggcagc gggtggcggt cggggttgtt
      481 tctggcggag gtgctgctga tgatgtaatt aaagtaggcg gtcttgagac ggcggatggt
      541 cgaggtgagg tgtggcaggc ttgagatcca gctgttgggg tgagtactcc ctctcaaaag
      601 cgggcattac ttctgcgcta agattgtcag tttccaaaaa cgaggaggat ttgatattca
      661 cctggcccga tctggccata cacttgagtg acaatgacat ccactttgcc tttctctcca
      721 caggtgtcca ctcccaggtc caagtttgcc accatggagt ttcagaccca ggtactcatg
      781 tccctgctgc tctgcatgtc tggtgcggcc gccaccgaag gaccttggcc tctgtctgtg
      841 caggccgccc acagatccac cgatgcccac gccgagggcc ccagcatcca ccccagaaag
      901 tactacctgg ggaagtacgg cgcccactgc atccggaacc tgaccgagtt cgtggccagc
      961 gaggacgccg ccgagagact ggcctgtttc tctggcagag gcgtgcgggt ggccctgctg
     1021 gaaacaggcc tgtgtgccgg aatcgccgag cggagcagaa acagcgtgac ctgcaccagc
     1081 gtggtgcccg gcgtggcctg tgaagatgcc ggctgtgccc acggcacccg ctctgtgtca
     1141 gtgctggccg ggcagctgtc cgtgtccgcc cctctgccta gagagcccct ggaagagaca
     1201 gcctggcgga acagagagac acacctggcc ctggccgacc agtacatcgg cctggcccct
     1261 gacagcaccg tccgggtgct gcggatcttc gacagacagg gccggaccaa ccggcggcat
     1321 ctggccagag cactggacct gctgctgaga gaggccgagg acggcgaagc cggcagactg
     1381 cacagcgcct ggaataccag cagcgcccac atccggcgga gggacgagac agtggacgtg
     1441 atcagcctga gctacggcag caaggactac cacagcagcc cccaggtgca ggaccggctg
     1501 taccggctga tgcacgagca cggcgtgatc gtggtggccg ctgccggcaa cgatggcgcc
     1561 agattcggat ctgtgcggag ccctgccgac atgccaggcg tgctggctgt gggcgccctg
     1621 agaatggaat ggcacggcag aagcgcccag accacccagg ccagcagaac cctgggcgat
     1681 ctggcctcta gcctggctgc tggcggcgga cacaagagcg tggcccactt cagcggcagg
     1741 ggccctacca cctgggagct gccttttggc gccggaaggg ccaagcccga tctggtggct
     1801 ctgggacagc acgtgtgggc cgtgcagggg gtgtcagccg ccgctttttc tgctgccgcc
     1861 cctagagcca gatccgcctc tcccgcactg cagctgagat ctgccagcgg cacctctatc
     1921 gccgctccca tcgtggctgg ggtggtggca ctgtgcctgg aagccgcctg gtccagcaca
     1981 tctgccgtcg ccctggctca gaacatgaca ggcggaggca gcttcctgga cgtgcggcag
     2041 aacaggctgg cccgggtgtc cgactccctg agagtgcggg aagtgatcct gcggaccgcc
     2101 gtgcctctgg gcgaagctgc tgctagcctg cctccttggc cccactctgc ccccaccacc
     2161 cccctgcact ctgatagcag cagcaccgcc tccgaggccc accctagaag agtcctggct
     2221 ggcgtgcccc tgctgaagct gtacgccggc tacctgagac tgagcagagt gtccatcctg
     2281 tctcagggcg ctggcgaggt gcagcctctg agggccctgc acgccattgt ggccaaggca
     2341 gccgccagct ccgccatcga tgcccctgag gccctgagga actgcgcctc cttcgccatc
     2401 cctacctgcg tgcggatcgg ctaccggatc cccagatgca gcaacagcag ctccagctct
     2461 agctcctcca gcggcgacat gagcaccccc cagagctgcc agccccccca cgatcctgat
     2521 ggcaaggccc atggcgatag ccccctgccc ggcagatccg gcatgagcct ggatgcccca
     2581 ccagccgcct actggtggcc attcagcgac caggccgtgt accctggcgc cacccccgtg
     2641 ctgctcaaca tctccctgca cctgtgcccc cctagctctc ctgccgccgc agaccaggga
     2701 accagagatg ccgctgcagc cgcttctggc aagggggcca gaagaggcag caccacacac
     2761 atccacgtga cccatgtgat caccaaggtg tccggccacg tgcgggccag agaaggccac
     2821 ccctccagaa actccagcca ccagtgcgac gcctgccctc tgctgttcga gggcgccaag
     2881 agcagacaca gagccggctc cacctccccc acccggctgc tgaatctgac agctcggagc
     2941 gcctccccac gggcctcttg ctctgatggc gaagtggccg gcgaaggcgc cacagtgatg
     3001 ggccctagac acacacagac tgccgtgtgc agcagcgtga agcggtggtc cacccagcag
     3061 agatggattc accatctgct gagggtggcc accgagctga cagtggtggc tagcagaagc
     3121 cagacccggg ccaacgtggt ggaacagctg agccccccac ctaccagctt cagcctgtca
     3181 gtggccgtgt ctagccccgc cagcgctcac acccacctgt gttacgatgt ggccagggaa
     3241 accgtggcag tgtggcggaa gaagggcaag tccagccccg cttccgagaa ggatgccgca
     3301 gctggcagac gcagcgccga tggcgacgac ggccacgaca agcagcatag cagggacggc
     3361 gctcaggtgc ccgccgacca cgatagatgc gtgccactgt tccacctgtt cagagccctg
     3421 agcgtggaag gcgctctgca catcttcacc ggctcctcta gtagccagcc cgccctgacc
     3481 gtgcccttcg ccgtcagagt ggtcgagccc cctccaagag tgcagagagt gctgatcgac
     3541 accagcctgg actgtttcaa cccaaccacc gccaccagca acctgtttat cgccggcgac
     3601 gatccccacg agagcgacgc tgggggagct ggcgctgctc tgcggagagg cagacagaga
     3661 cagaggcacg agcgggccta tgccgaggcc tctggcggag atcacccctt caccaacctg
     3721 gcactgctgt acctgtatct gcggcacaca ctgggcatgg ccgtggaaac cttcccactg
     3781 ctgcacatgt ccacaatcgc cacctccatt gccgctagcc acagcccacc tctgtggccc
     3841 ccagccgaga ggaccagcaa tgtgtccagg accgcccaga gggctcccgc cggaacactg
     3901 gcccacattg gcaccctgat catcgtggac cccgagcgcc ccctgaccag aggcatgaga
     3961 aggctgctca ccagagccgt gctgggaggc ggagccgata gaagcgctga cggcctgaat
     4021 gtgctgctgg tcaccgactg gtactctgcc gacctggccg ctcagctgca ctgggctcgg
     4081 gacgagtccc tgtacgacgc cgaacggttc agccacatca acggcaccga cgccacagac
     4141 gaggtggcag gcgccgacgg ggatagggat gccgtgcggg atctgcgggc tctgaggcag
     4201 agcgagaacg gctctacacg gggcctggca ggcagctctc acgtgcccag ctggaaccgg
     4261 tggctgagcg aagtgactgt ggcctccggc tccgccgcca acagctccga ttgtgatggc
     4321 gcagctcccc tgggcagcga gggactgctg gatggaacca gcgaactgcc tttcgagctg
     4381 tctgagagca tcgtgatcga cggggctgtg ctggtggacg cagccgcacc cagaagaaga
     4441 agcgagcccg agggcaacgc ctttgccagc cacggcggaa ccgtgacaag cgccaaggtg
     4501 gtcgctctga gaagcctggg ccagctgtgt gctgccgggg tgctgagatg gcggctccct
     4561 gctcaggctg ctgtgcagag gcaggccggc agggctacag ccgtgggagc tagcaagggc
     4621 gtccgcgagc ccaatgtgta cccctccggc aatggcacct gtagcacagg cgacgccagc
     4681 aacacctctg ccatgcccag cacccaggaa ggcgaggccg tcatctgcaa cgtgatgccc
     4741 tcctgggccc agcagcagcg gcggctggtc aagagaaccg tggtgtccac ccgggacgga
     4801 tccgcaccag ctgccgtggt cgaagatgga tgggaggacg tgcccgtggc taacggcgac
     4861 ggcaggctcg tcagaatcgg cggactggaa gctgccgaag tgtctaccat ggctcctgct
     4921 gccacaggcg cctctgacgc cacccagagc ctgacacacg gcgtcctggg ctttctgacc
     4981 ctgccaccta gctccaccct ggtgtcaacc gtgtctgcca accggttccg gccaggccgg
     5041 atcgccatct ttaccgacag cgattgcctg tctgcctccg accaccacgc ccaggccgct
     5101 ctggatgaac tcgaagccct gctctaccct cccagcgcca gcccttccct gcctgtgacc
     5161 acatgcgcca catgggattg cttcagcagg accccagacg gacagcggct gctccaggcc
     5221 gagtctgccc agtccagcgt gtgtgtggaa gtcgtgaaag aactgctcct gtggctgcac
     5281 accggcaatc tgcatcggtg gcgggacagc gcacagctgc agtgtgaagc ccggacctgg
     5341 gctagagccc ggcacagaag cacagccgac accacaccag gggccggaag cgtgctcgag
     5401 cctaaagccg gcgaggactc tgtggaaggg gacagagaaa ccgccaactt cgacgagctg
     5461 ctgggcacag cccccgagag ggcccatgtg ggagaagtcg tgtggcgcct gtgggccagc
     5521 ctggtggaaa gcaccgagcg cgacgccgct tcctttctgg tggatggccc caaagagggc
     5581 acccggttcc agggcgatta cgccgcccat caggaaagcg ccgcagccaa cgtcatgcgg
     5641 gccctgatgg tcaaatacca cggcgattgg agagcccatc tcctggatca caacgtgtcc
     5701 cccccaagca ctggcgccgc tggggattcc tacagccagt ccctgcggcc tgccaggggc
     5761 agaagatctg gcattgccac cgtccagcag cctgaacagc acattagcct gatcgagagc
     5821 ctgctccccc tgtgcggcat gctgaacgct ctcagatggc tgcagcatcc tggcgcgccc
     5881 ggcagcctgc accacatcct ggacgcccag aagatgctgt ggaaccacag ggacaggaac
     5941 ctgcctcctc tggcccctct gggcccccac caccatcacc accattgagt ctagacacgt
     6001 gattaattaa ggatcccccg acctcgacct ctggctaata aaggaaattt attttcattg
     6061 caatagtgtg ttggaatttt ttgtgtctct cactcggaag gacatatggg agggcaaatc
     6121 atttggtcga gatccctcgg agatctctag ctagaggatc gatccccgcc ccggacgaac
     6181 taaacctgac tacgacatct ctgccccttc ttcgcggggc agtgcatgta atcccttcag
     6241 ttggttggta caacttgcca actgggccct gttccacatg tgacacgggg ggggaccaaa
     6301 cacaaagggg ttctctgact gtagttgaca tccttataaa tggatgtgca catttgccaa
     6361 cactgagtgg ctttcatcct ggagcagact ttgcagtctg tggactgcaa cacaacattg
     6421 cctttatgtg taactcttgg ctgaagctct tacaccaatg ctgggggaca tgtacctccc
     6481 aggggcccag gaagactacg ggaggctaca ccaacgtcaa tcagaggggc ctgtgtagct
     6541 accgataagc ggaccctcaa gagggcatta gcaatagtgt ttataaggcc cccttgttaa
     6601 ccctaaacgg gtagcatatg cttcccgggt agtagtatat actatccaga ctaaccctaa
     6661 ttcaatagca tatgttaccc aacgggaagc atatgctatc gaattagggt tagtaaaagg
     6721 gtcctaagga acagcgatat ctcccacccc atgagctgtc acggttttat ttacatgggg
     6781 tcaggattcc acgagggtag tgaaccattt tagtcacaag ggcagtggct gaagatcaag
     6841 gagcgggcag tgaactctcc tgaatcttcg cctgcttctt cattctcctt cgtttagcta
     6901 atagaataac tgctgagttg tgaacagtaa ggtgtatgtg aggtgctcga aaacaaggtt
     6961 tcaggtgacg cccccagaat aaaatttgga cggggggttc agtggtggca ttgtgctatg
     7021 acaccaatat aaccctcaca aaccccttgg gcaataaata ctagtgtagg aatgaaacat
     7081 tctgaatatc tttaacaata gaaatccatg gggtggggac aagccgtaaa gactggatgt
     7141 ccatctcaca cgaatttatg gctatgggca acacataatc ctagtgcaat atgatactgg
     7201 ggttattaag atgtgtccca ggcagggacc aagacaggtg aaccatgttg ttacactcta
     7261 tttgtaacaa ggggaaagag agtggacgcc gacagcagcg gactccactg gttgtctcta
     7321 acacccccga aaattaaacg gggctccacg ccaatggggc ccataaacaa agacaagtgg
     7381 ccactctttt ttttgaaatt gtggagtggg ggcacgcgtc agcccccaca cgccgccctg
     7441 cggttttgga ctgtaaaata agggtgtaat aacttggctg attgtaaccc cgctaaccac
     7501 tgcggtcaaa ccacttgccc acaaaaccac taatggcacc ccggggaata cctgcataag
     7561 taggtgggcg ggccaagata ggggcgcgat tgctgcgatc tggaggacaa attacacaca
     7621 cttgcgcctg agcgccaagc acagggttgt tggtcctcat attcacgagg tcgctgagag
     7681 cacggtgggc taatgttgcc atgggtagca tatactaccc aaatatctgg atagcatatg
     7741 ctatcctaat ctatatctgg gtagcatagg ctatcctaat ctatatctgg gtagcatatg
     7801 ctatcctaat ctatatctgg gtagtatatg ctatcctaat ttatatctgg gtagcatagg
     7861 ctatcctaat ctatatctgg gtagcatatg ctatcctaat ctatatctgg gtagtatatg
     7921 ctatcctaat ctgtatccgg gtagcatatg ctatcctaat agagattagg gtagtatatg
     7981 ctatcctaat ttatatctgg gtagcatata ctacccaaat atctggatag catatgctat
     8041 cctaatctat atctgggtag catatgctat cctaatctat atctgggtag cataggctat
     8101 cctaatctat atctgggtag catatgctat cctaatctat atctgggtag tatatgctat
     8161 cctaatttat atctgggtag cataggctat cctaatctat atctgggtag catatgctat
     8221 cctaatctat atctgggtag tatatgctat cctaatctgt atccgggtag catatgctat
     8281 cctcatgata agctgtcaaa catgagaatt ttcttgaaga cgaaagggcc tcgtgatacg
     8341 cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt
     8401 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     8461 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     8521 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     8581 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     8641 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     8701 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     8761 tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     8821 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     8881 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     8941 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     9001 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     9061 tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     9121 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     9181 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     9241 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     9301 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     9361 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     9421 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     9481 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     9541 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     9601 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     9661 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     9721 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     9781 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     9841 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     9901 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     9961 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
    10021 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
    10081 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
    10141 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
    10201 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
    10261 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
    10321 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
    10381 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
    10441 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
    10501 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagctcta
    10561 gctagaggtc gaccaattct catgtttgac agcttatcat cgcagatccg ggcaacgttg
    10621 ttgccattgc tgcaggcgca gaactggtag gtatggaaga tctatacatt gaatcaatat
    10681 tggcaattag ccatattagt cattggttat atagcataaa tcaatattgg ctattggcca
    10741 ttgcatacgt tgtatctata tcataatatg tacatttata ttggctcatg tccaatatga
    10801 ccgccatgtt gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta
    10861 gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc
    10921 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
    10981 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
    11041 gcagtacatc aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa tgacggtaaa
    11101 tggcccgcct ggcattatgc ccagtacatg accttacggg actttcctac ttggcagtac
    11161 atctacgtat tagtcatcgc tattaccatg
//
