LOCUS       sequence-274551-        5895 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express Bcl6 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5895)
  AUTHORS   Misawa T, SoRelle JA, Choi JH, Yue T, Wang KW, McAlpine W, Wang J, 
            Liu A, Tabeta K, Turer EE, Evers B, Nair-Gill E, Poddar S, Su L, Ou 
            F, Yu L, Russell J, Ludwig S, Zhan X, Hildebrand S, Li X, Tang M, 
            Murray AR, Moresco EMY, Beutler B
  TITLE     Mutual inhibition between Prkd2 and Bcl6 controls T follicular 
            helper cell differentiation.
  JOURNAL   Sci Immunol. 2020 Jan 24;5(43):eaaz0085. doi: 
            10.1126/sciimmunol.aaz0085.
  PUBMED    31980486
REFERENCE   2  (bases 1 to 5895)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Immunol."; date: "2020-01-24"; volume: "5(43):eaaz0085. doi"; pages:
            " 10.1126/sciimmunol.aaz0085"
COMMENT     Sequence Label: pCMV-HA_N-human Bcl6
FEATURES             Location/Qualifiers
     source          1..5895
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        44..347
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        348..551
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     502..522
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     548..572
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          689..785
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     CDS             849..875
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    3051..3185
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3075..3094)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3129..3148
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(3296..3312)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3296..3312)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3309..3331)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3320..3336
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3344..3350,3351..3368,3369..3374))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3389..3410
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3527..3544)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3698..4286)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3778..3797)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4457..5248,5249..5317))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5080..5099
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5318..5422)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5490..5508
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(5546..5568)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5668..5687
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     join(5881..5895,1..8)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     join(5895,1..17)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
ORIGIN
        1 gtaaaacgac ggccagtgag ttcgagcttg catgcctgca ggtcgttaca taacttacgg
       61 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
      121 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
      181 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
      241 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
      301 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt
      361 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc
      421 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
      481 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
      541 taagcagagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg
      601 acctccatag aagacaccgg gaccgatcca gcctccggac tctagaggat ccggtactag
      661 aggaactgaa aaaccagaaa gttaactggt aagtttagtc tttttgtctt ttatttcagg
      721 tcccggatcc ggtggtggtg caaatcaaag aactgctcct cagtggatgt tgcctttact
      781 tctaggcctg tacggaagtg ttacttctgc tctaaaagct gcggaattgt acccgcgggc
      841 ccaccatgta cccatacgat gttccagatt acgctcttat ggccatggag gcccgaattc
      901 ggtcgaccga gatctctcga atggcctcgc cggctgacag ctgtatccag ttcacccgcc
      961 atgccagtga tgttcttctc aaccttaatc gtctccggag tcgagacatc ttgactgatg
     1021 ttgtcattgt tgtgagccgt gagcagttta gagcccataa aacggtcctc atggcctgca
     1081 gtggcctgtt ctatagcatc tttacagacc agttgaaatg caaccttagt gtgatcaatc
     1141 tagatcctga gatcaaccct gagggattct gcatcctcct ggacttcatg tacacatctc
     1201 ggctcaattt gcgggagggc aacatcatgg ctgtgatggc cacggctatg tacctgcaga
     1261 tggagcatgt tgtggacact tgccggaagt ttattaaggc cagtgaagca gagatggttt
     1321 ctgccatcaa gcctcctcgt gaagagttcc tcaacagccg gatgctgatg ccccaagaca
     1381 tcatggccta tcggggtcgt gaggtggtgg agaacaacct gccactgagg agcgcccctg
     1441 ggtgtgagag cagagccttt gcccccagcc tgtacagtgg cctgtccaca ccgccagcct
     1501 cttattccat gtacagccac ctccctgtca gcagcctcct cttctccgat gaggagtttc
     1561 gggatgtccg gatgcctgtg gccaacccct tccccaagga gcgggcactc ccatgtgata
     1621 gtgccaggcc agtccctggt gagtacagcc ggccgacttt ggaggtgtcc cccaatgtgt
     1681 gccacagcaa tatctattca cccaaggaaa caatcccaga agaggcacga agtgatatgc
     1741 actacagtgt ggctgagggc ctcaaacctg ctgccccctc agcccgaaat gccccctact
     1801 tcccttgtga caaggccagc aaagaagaag agagaccctc ctcggaagat gagattgccc
     1861 tgcatttcga gccccccaat gcacccctga accggaaggg tctggttagt ccacagagcc
     1921 cccagaaatc tgactgccag cccaactcgc ccacagagtc ctgcagcagt aagaatgcct
     1981 gcatcctcca ggcttctggc tcccctccag ccaagagccc cactgacccc aaagcctgca
     2041 actggaagaa atacaagttc atcgtgctca acagcctcaa ccagaatgcc aaaccagagg
     2101 ggcctgagca ggctgagctg ggccgccttt ccccacgagc ctacacggcc ccacctgcct
     2161 gccagccacc catggagcct gagaaccttg acctccagtc cccaaccaag ctgagtgcca
     2221 gcggggagga ctccaccatc ccacaagcca gccggctcaa taacatcgtt aacaggtcca
     2281 tgacgggctc tccccgcagc agcagcgaga gccactcacc actctacatg caccccccga
     2341 agtgcacgtc ctgcggctct cagtccccac agcatgcaga gatgtgcctc cacaccgctg
     2401 gccccacgtt ccctgaggag atgggagaga cccagtctga gtactcagat tctagctgtg
     2461 agaacggggc cttcttctgc aatgagtgtg actgccgctt ctctgaggag gcctcactca
     2521 agaggcacac gctgcagacc cacagtgaca aaccctacaa gtgtgaccgc tgccaggcct
     2581 ccttccgcta caagggcaac ctcgccagcc acaagaccgt ccataccggt gagaaaccct
     2641 atcgttgcaa catctgtggg gcccagttca accggccagc caacctgaaa acccacactc
     2701 gaattcactc tggagagaag ccctacaaat gcgaaacctg cggagccaga tttgtacagg
     2761 tggcccacct ccgtgcccat gtgcttatcc acactggtga gaagccctat ccctgtgaaa
     2821 tctgtggcac ccgtttccgg caccttcaga ctctgaagag ccacctgcga atccacacag
     2881 gagagaaacc ttaccattgt gagaagtgta acctgcattt ccgtcacaaa agccagctgc
     2941 gacttcactt gcgccagaag catggcgcca tcaccaacac caaggtgcaa taccgcgtgt
     3001 cagccactga cctgcctccg gagctcccca aagcctgctg aggccgcggg gatccagaca
     3061 tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct
     3121 ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac
     3181 aagttaacaa taacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg
     3241 ttttttcgga tcctctagag tcgatctgca ggcatgctag cttggcgtaa tcatggtcat
     3301 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
     3361 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
     3421 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
     3481 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     3541 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     3601 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     3661 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     3721 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     3781 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     3841 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     3901 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     3961 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     4021 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     4081 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     4141 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     4201 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     4261 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     4321 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     4381 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     4441 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     4501 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     4561 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     4621 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     4681 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     4741 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     4801 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     4861 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     4921 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     4981 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     5041 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     5101 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     5161 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     5221 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     5281 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     5341 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     5401 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa
     5461 ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg
     5521 cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag
     5581 cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg
     5641 gcgggtgtcg gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc
     5701 atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgccatt
     5761 cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac
     5821 gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt
     5881 cccagtcacg acgtt
//
