LOCUS       sequence-274553-        5898 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express mouse Bcl6 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5898)
  AUTHORS   Misawa T, SoRelle JA, Choi JH, Yue T, Wang KW, McAlpine W, Wang J, 
            Liu A, Tabeta K, Turer EE, Evers B, Nair-Gill E, Poddar S, Su L, Ou 
            F, Yu L, Russell J, Ludwig S, Zhan X, Hildebrand S, Li X, Tang M, 
            Murray AR, Moresco EMY, Beutler B
  TITLE     Mutual inhibition between Prkd2 and Bcl6 controls T follicular 
            helper cell differentiation.
  JOURNAL   Sci Immunol. 2020 Jan 24;5(43):eaaz0085. doi: 
            10.1126/sciimmunol.aaz0085.
  PUBMED    31980486
REFERENCE   2  (bases 1 to 5898)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Immunol."; date: "2020-01-24"; volume: "5(43):eaaz0085. doi"; pages:
            " 10.1126/sciimmunol.aaz0085"
COMMENT     Sequence Label: pCMV-HA_N-mouse Bcl6
FEATURES             Location/Qualifiers
     source          1..5898
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     505..529
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          646..742
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     CDS             806..832
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    3011..3145
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3035..3054)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3089..3108
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(3256..3272)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3256..3272)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3269..3291)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3280..3296
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3304..3310,3311..3328,3329..3334))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3349..3370
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3487..3504)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3658..4246)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3738..3757)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4417..5208,5209..5277))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5040..5059
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5278..5382)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5450..5468
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(5506..5528)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5628..5647
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5841..5863
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5855..5872
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5856..5872
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcg cctggagacg
      541 ccatccacgc tgttttgacc tccatagaag acaccgggac cgatccagcc tccggactct
      601 agaggatccg gtactagagg aactgaaaaa ccagaaagtt aactggtaag tttagtcttt
      661 ttgtctttta tttcaggtcc cggatccggt ggtggtgcaa atcaaagaac tgctcctcag
      721 tggatgttgc ctttacttct aggcctgtac ggaagtgtta cttctgctct aaaagctgcg
      781 gaattgtacc cgcgggccca ccatgtaccc atacgatgtt ccagattacg ctcttatggc
      841 catggaggcc cgaattcggt cgaccgagat ctctcgaatg gcctccccgg ctgacagctg
      901 tatccagttt acccggcacg ctagtgatgt tcttctcaac cttaatcgcc tccggagtcg
      961 ggacatcttg acggacgttg tcatcgtggt gagccgtgag cagtttagag cccataagac
     1021 agtgctcatg gcctgcagcg gcctgttcta cagtatcttc actgaccagt tgaaatgcaa
     1081 ccttagtgta atcaatctag atcctgaaat cagccctgag gggttttgca tcctcctgga
     1141 cttcatgtac acatctaggc tcaacctgag ggaaggcaat atcatggcgg tgatgaccac
     1201 agccatgtac ctgcagatgg agcatgttgt cgacacatgc aggaagttca tcaaggccag
     1261 tgaagcagaa atggcccctg cacttaaacc tccccgtgaa gagttcctga acagccggat
     1321 gctgatgccc catgacatca tggcctaccg aggtcgtgag gtcgtggaga acaatatgcc
     1381 actgagaaat actcccgggt gtgagagcag agcttttgct cctcctctgt acagtggcct
     1441 gtcaacacca ccagcctctt atcccatgta cagccatctc ccgctcagca ccttcctctt
     1501 ctctgatgag gagctccgag atgccccccg aatgcctgtg gccaaccctt ttcccaagga
     1561 gcgtgccctc ccctgcgaca gtgccaggca agtccctaat gagtatagca ggccagccat
     1621 ggaggtgtcc cccagtttgt gtcacagcaa catctactcg cccaaggagg cagtcccaga
     1681 ggaggctcgg agtgacatac actacagtgt gcctgagggc cccaagcctg ctgtcccttc
     1741 tgctcggaat gctccatact tcccctgtga caaagccagc aaagaagaag agagaccttc
     1801 ttcggaggat gagattgccc tgcatttcga gccccccaat gcacccttga accggaaggg
     1861 tctggttagt ccccagagtc cccagaaatc cgactgccag cccaactcac ccacagagtc
     1921 ctgcagcagc aagaacgcct gcatccttca ggcctctggc tctccgccag ccaagagccc
     1981 cactgacccg aaagcctgca actggaagaa gtataagttc atcgttctca acagcctcaa
     2041 tcagaatgcc aaacccgagg gctctgagca ggcagagctg ggtcgcctct cccctcgagc
     2101 ctaccctgca ccgcccgctt gccagccgcc tatggagccc gcgaaccttg atctccagtc
     2161 cccgaccaag ctcagtgcca gtggggagga ctctaccatc ccccaagcca gccggctcaa
     2221 taatctcgtg aacaggtccc tggcaggctc cccccgaagc agcagtgaga gtcactcacc
     2281 actctacatg caccccccaa agtgcacatc ctgcggctct cagtccccac agcatacaga
     2341 gatgtgcctc catactgctg ggcccacgtt cccggaggag atgggggaaa cccagtcaga
     2401 gtattcggat tctagctgtg agaatgggac cttcttctgc aacgaatgtg actgccgttt
     2461 ctctgaggag gcctcgctca agaggcacac gctgcagacg cacagtgaca aaccatacaa
     2521 atgtgatcgc tgccaggcct ccttccgcta caagggcaac ctcgccagcc acaagactgt
     2581 ccacacgggt gagaaaccct atcgctgtaa catttgtgga gcgcagttca atcggccagc
     2641 caacctgaag acccacactc gaattcactc tggagaaaag ccctacaaat gtgaaacctg
     2701 tggggccagg tttgttcagg tggcccacct ccgtgcccac gtgctcatcc acactggaga
     2761 gaagccgtac ccctgtgaaa tctgtggcac tcgcttccgg caccttcaga ctctgaagag
     2821 ccatctgcgc atccacacag gagagaaacc ttaccattgt gagaagtgta acctgcactt
     2881 tcgtcacaaa agccaactgc gacttcattt gcgccagaag cacggcgcca tcaccaacac
     2941 caaggtgcaa taccgcgtgt cggccgctga cctgcctccg gagctcccca aagcctgctg
     3001 aggccgcggg gatccagaca tgataagata cattgatgag tttggacaaa ccacaactag
     3061 aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac
     3121 cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt
     3181 tcagggggag gtgtgggagg ttttttcgga tcctctagag tcgatctgca ggcatgctag
     3241 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     3301 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     3361 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     3421 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     3481 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     3541 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     3601 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     3661 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     3721 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     3781 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     3841 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     3901 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     3961 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     4021 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     4081 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     4141 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     4201 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     4261 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     4321 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     4381 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     4441 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     4501 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     4561 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     4621 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     4681 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     4741 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     4801 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     4861 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     4921 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     4981 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     5041 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     5101 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     5161 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     5221 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     5281 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     5341 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     5401 gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat
     5461 cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca
     5521 gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca
     5581 gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg catcagagca
     5641 gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa
     5701 ataccgcatc aggcgccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt
     5761 gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag
     5821 ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgagttcga
     5881 gcttgcatgc ctgcaggt
//
