LOCUS       sequence-274577-        4482 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  cI repressor expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4482)
  AUTHORS   Neil K, Allard N, Grenier F, Burrus V, Rodrigue S
  TITLE     Highly efficient gene transfer in the mouse gut microbiota is 
            enabled by the Incl(2) conjugative plasmid TP114.
  JOURNAL   Commun Biol. 2020 Sep 22;3(1):523. doi: 10.1038/s42003-020-01253-0.
  PUBMED    32963323
REFERENCE   2  (bases 1 to 4482)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2020-09-22"; volume: "3(1):523. doi"; pages: " 
            10.1038/s42003-020-01253-0"
COMMENT     Sequence Label: pFG036
FEATURES             Location/Qualifiers
     source          1..4482
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1928..1948
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     1949..1968
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1949..1967
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     1975..1992
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     2046..2064
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2102..2124)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2224..2243
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     CDS             complement(2382..3095)
                     /codon_start=1
                     /gene="cIts"
                     /product="temperature-sensitive variant of the phage lambda
                     repressor"
                     /label=lambda repressor (ts)
                     /note="thermosensitivity is conferred by the A67T mutation"
                     /translation="MSTKKKPLTQEQLEDARRLKAIYEKKKNELGLSQESVADKMGMGQ
                     SGVGALFNGINALNAYNAALLTKILKVSVEEFSPSIAREIYEMYEAVSMQPSLRSEYEY
                     PVFSHVQAGMFSPELRTFTKGDAERWVSTTKKASDSAFWLEVEGNSMTAPTGSKPSFPD
                     GMLILVDPEQAVEPGDFCIARLGGDEFTFKKLIRDSGQVFLQPLNPQYPMIPCNESCSV
                     VGKVIASQWPEETFG"
     primer_bind     complement(3296..3313)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3467..4055)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3547..3566)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctagatgaaa atcataaata tcggtgtatt agctcacgtt gatgcaggaa aaacaacatt
       61 aactgaatca cttttatata actctggtgc aattactgaa cttggttcag tagataaagg
      121 tactactcgt actgataata cattattaga acgtcaacgt ggaatcacaa ttcaaacagg
      181 tatcacatct tttcaatggg aaaatacaaa agtaaatatt atagatacac ctggacacat
      241 ggatttcctt gcagaagtat accgtagtct ttcagtatta gatggtgcta ttttacttat
      301 cagcgctaaa gatggagttc aagctcaaac tcgtatctta tttcacgcat tacgtaaaat
      361 gggtattcca acaattttct ttataaacaa aattgaccaa aacggaattg atttaagtac
      421 agtttatcaa gatatcaaag aaaaactttc tgctgaaatc gttattaaac aaaaagttga
      481 attataccca aacgtttgcg taacaaattt tactgaatca gaacaatggg atacagttat
      541 agaaggtaat gatgatttat tagaaaaata catgtcaggt aaatcattag aagcattaga
      601 attagaacaa gaagaaagta ttcgtttcca aaactgttct ttattccctt tataccatgg
      661 aagcgctaaa agtaacatag gtattgataa cttaattgaa gttattacta acaaatttta
      721 ctcttcaact catcgtggtc cttctgaatt atgcggtaac gttttcaaaa ttgaatatac
      781 aaaaaaacgt caacgtttag cttatatacg tctttatagt ggtgttttac atttacgtga
      841 tagtgttcgt gttagtgaaa aagaaaagat taaagttaca gaaatgtata cttctattaa
      901 cggtgaatta tgcaaaattg accgtgcata ttcaggtgaa attgtaattt tacaaaacga
      961 atttcttaaa cttaatagtg tacttggtga cacaaaactt ttaccacaac gtaagaaaat
     1021 tgaaaatcca cacccattac ttcaaacaac agtagaacca agcaaacctg aacaacgtga
     1081 aatgctttta gatgctcttt tagaaattag tgactctgac ccacttttac gttactatgt
     1141 agattctact actcatgaaa ttattctttc tttccttggt aaagttcaaa tggaagttat
     1201 ttctgcatta ttacaagaaa aatatcatgt tgaaatcgaa ttaaaagaac ctactgtaat
     1261 ttatatggaa cgtccattaa aaaatgctga atatacaatt catattgaag ttccaccaaa
     1321 tccattttgg gcttctattg gtctttctgt ttctccactt ccacttggta gcggaatgca
     1381 atatgaaagt agcgtaagtt taggttatct taatcaaagt ttccaaaacg cagttatgga
     1441 aggtattcgt tacggttgcg aacaaggttt atacggttgg aatgttacag actgcaaaat
     1501 ctgttttaag tatggacttt actattcacc tgtatcaaca cctgctgact ttcgtatgct
     1561 tgcaccaatt gttttagaac aagttttaaa gaaagctgga actgaacttt tagaaccata
     1621 cctttctttt aaaatctatg caccacaaga atacttaagt cgtgcttata acgatgcacc
     1681 taaatactgt gctaatattg ttgatactca attaaagaac aacgaagtaa ttttaagcgg
     1741 agaaattcct gcacgttgta ttcaagaata tcgtagtgat ttaacatttt tcactaatgg
     1801 acgttctgtt tgcttaactg aattaaaagg ttatcatgtt actactggtg aacctgtatg
     1861 ccaaccacgt cgtcctaata gtcgtattga taaagttcgt tatatgttca acaaaatcac
     1921 ataataatac tagtagcggc cgctgcagta atacgactca ctatagggca tacgatttag
     1981 gtgacactat agtagggata acagggtaat ttagttactt acctagcatg acattaacct
     2041 ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa
     2101 acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga
     2161 gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact
     2221 atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca
     2281 gatgcgtaag gagaaaatac cgcatcaggc gccattcgcc attcaggctg cgcaactgtt
     2341 gggaagggcg atcggtgcgg gccttttcgc tattacgcca gtcagccaaa cgtctcttca
     2401 ggccactgac tagcgataac tttccccaca acggaacaac tctcattgca tgggatcatt
     2461 gggtactgtg ggtttagtgg ttgtaaaaac acctgaccgc tatccctgat cagtttcttg
     2521 aaggtaaact catcaccccc aagtctggct atgcagaaat cacctggctc aacagcctgc
     2581 tcagggtcaa cgagaattaa cattccgtca ggaaagcttg gcttggagcc tgttggtgcg
     2641 gtcatggaat taccttcaac ctcaagccag aatgcagaat cactggcttt tttggttgtg
     2701 cttacccatc tctccgcatc acctttggta aaggttctaa gctcaggtga gaacatccct
     2761 gcctgaacat gagaaaaaac agggtactca tactcacttc taagtgacgg ctgcatacta
     2821 accgcttcat acatctcgta gatttctctg gcgattgaag ggctaaattc ttcaacgcta
     2881 actttgagaa tttttgtaag caatgcggcg ttataagcat ttaatgcatt gatgccatta
     2941 aataaagcac caacgcctga ctgccccatc cccatcttgt ctgcgacaga ttcctgggat
     3001 aagccaagtt catttttctt tttttcataa attgctttaa ggcgacgtgc gtcctcaagc
     3061 tgctcttgtg ttaatggttt cttttttgtg ctcattagaa actgtccttc gcatgattaa
     3121 gatgttcagt acgaaaattg ctttcattgt tgatctcctt tttaagtgaa cttgggccct
     3181 ccacacatta tacagaaaaa ttttcctgat gtcaactggc gaaaagatcc ctgcattaat
     3241 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     3301 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     3361 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     3421 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     3481 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     3541 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     3601 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     3661 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     3721 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     3781 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     3841 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     3901 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     3961 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     4021 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     4081 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg aattcggcca
     4141 gtctacatgt actctttttg ataaaaaatt ggagattcct ttacaaatat gctcttacgt
     4201 gctattattt aagtgactat ttaaaaggag ttaataaata tgcggcaagg tattcttaaa
     4261 taaactgtca atttgatagc gggaacaaat aattagatgt ccttttttag gagggcttag
     4321 ttttttgtac ccagtttaag aataccttta tcatgtgatt ctaaagtatc cagagaatat
     4381 ctgtatgctt tgtataccta tggttatgca taaaaatccc ggtgataaaa gtatttatca
     4441 ctgggatttt tatgcccttt tgggtttttg aatggaggaa at
//
