LOCUS       sequence-274583-        7443 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express tTALE-mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7443)
  AUTHORS   Shin E, Kim W, Lee S, Bae J, Kim S, Ko W, Seo HS, Lim S, Lee HS, Jo 
            K
  TITLE     Truncated TALE-FP as DNA Staining Dye in a High-salt Buffer.
  JOURNAL   Sci Rep. 2019 Nov 20;9(1):17197. doi: 10.1038/s41598-019-53722-0.
  PUBMED    31748571
REFERENCE   2  (bases 1 to 7443)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2019-11-20"; volume: "9(1):17197. doi"; pages: " 
            10.1038/s41598-019-53722-0"
COMMENT     Sequence Label: pET-TALE-mCherry-15b
FEATURES             Location/Qualifiers
     source          1..7443
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..20
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1..19
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    20..44
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             59..81
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             100..117
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             127..144
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             1183..1893
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDQMAIIKEFMRFKVHMEGSVDGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1328..1346)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1596..1615
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     1790..1813
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(1944..1962)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      1958..2005
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(2180..2185,2186..2202,2203..2208))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(2235..2253)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2321..2425
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2426..2494,2495..3286)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2644..2663)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3457..4045
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3946..3965
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4199..4216
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    4231..4370
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(4296..4315)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4456..4478
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(4472..4663)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(5975..7057)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     7019..7038
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(7058..7135)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     7341..7360
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt
       61 tgtttaactt taagaaggag atataccatg ggcagcagcc atcatcatca tcatcacagc
      121 agcggcctgg tgccgcgcgg cagccatatg gatctacgca cgctcggcta cagccagcag
      181 caacaggaga agatcaaacc gaaggttcgt tcgacagtgg cgcagcacca cgaggcactg
      241 gtcggccatg ggtttacaca cgcgcacatc gttgcgctca gccaacaccc ggcagcgtta
      301 gggaccgtcg ctgtcaagta tcaggacatg atcgcagcgt tgccagaggc gacacacgaa
      361 gcgatcgttg gcgtcggcaa acagtggtcc ggcgcacgcg ctctggaggc cttgctcacg
      421 gtggcgggag agttgagagg tccaccgtta cagttggaca caggccaact tctcaagatt
      481 gcaaaacgtg gcggcgtgac cgcagtggag gcagtgcatg catggcgcaa tgcactgacg
      541 ggtgcccccc tgaacctgac tccagcacag gtggtggcta ttgcctccaa taatggggga
      601 aaacaggctc tcgaaacagt tcaaagactt ctgcctgtct tgtgccaaga tcatggactt
      661 acacccgcac aagtcgttgc aatcgctagc aatggcggtg ggaagcaagc cctggagaca
      721 gttcaaaggc tccttccagt cctgtgtcag gcacacgggc ttacccctga tcaagtggtc
      781 gcaattgcct cacatgacgg cgggaaacag gcattggaaa ctgtgcagcg cctgctcccc
      841 gttctttgcc aggatcatgg gttgacacct gcacaagttg ttgcaattgc aagcaacggc
      901 ggcggaaaac aggcactgga aactgtgcaa agactcctgc cagttttgtg ccaggctcat
      961 ggcctgactc cagaccaggt cgttgctatt gccagcaata acggaggtaa gcaagcattg
     1021 gagactgttc agcggctcct ccctgtcctc tgtcaggccc acgggctgac tcctgcacag
     1081 gtggtggcca tcgcttcaaa cggggggggg aagcaagccc tcgaaactgt tcaacggttg
     1141 ttgcctgttt tgtgtcagga tcatgggggc ggctctggcg gcatggtgag caagggcgag
     1201 gaggaccaga tggccatcat caaggagttc atgcgcttca aggtgcacat ggagggctcc
     1261 gtggacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc
     1321 cagaccgcca agctgaaggt gaccaagggc ggccccctgc ccttcgcctg ggacatcctg
     1381 tcccctcagt tcatgtacgg ctccaaggcc tacgtgaagc accccgccga catccccgac
     1441 tacttgaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac
     1501 ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg acggcgagtt catctacaag
     1561 gtgaagctgc gcggcaccaa cttcccctcc gacggccccg taatgcagaa gaagaccatg
     1621 ggctgggagg cctcctccga gcggatgtac cccgaggacg gcgccctgaa gggcgagatc
     1681 aagcagaggc tgaagctgaa ggacggcggc cactacgacg ccgaggtcaa gaccacctac
     1741 aaggccaaga agcccgtgca gctgcccggc gcctacaacg tgaacatcaa gctggacatc
     1801 acctcccaca acgaggacta caccatcgtg gaacagtacg agcgcgccga gggccgccac
     1861 tccaccggcg gcatggacga gctgtacaag taaggatccg gctgctaaca aagcccgaaa
     1921 ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc
     1981 taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atcccgcaag
     2041 aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg
     2101 aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt
     2161 aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc
     2221 ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat
     2281 ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     2341 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     2401 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     2461 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     2521 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     2581 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     2641 tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg
     2701 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     2761 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     2821 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     2881 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     2941 aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt
     3001 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     3061 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     3121 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     3181 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     3241 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     3301 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     3361 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     3421 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     3481 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     3541 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     3601 tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     3661 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     3721 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     3781 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     3841 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     3901 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     3961 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     4021 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     4081 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     4141 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
     4201 cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct
     4261 gtgcggtatt tcacaccgca atggtgcact ctcagtacaa tctgctctga tgccgcatag
     4321 ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc
     4381 cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac
     4441 aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac
     4501 gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg
     4561 cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga
     4621 taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag
     4681 ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac
     4741 gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg
     4801 tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata
     4861 cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa
     4921 tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca
     4981 ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc
     5041 gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca
     5101 acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc
     5161 gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg
     5221 cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag
     5281 cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa
     5341 cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt
     5401 gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccaatgatcg aagttaggct
     5461 ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga
     5521 cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg
     5581 gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc
     5641 catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa
     5701 ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc
     5761 gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac
     5821 gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca
     5881 ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgagatc ccggtgccta
     5941 atgagtgagc taacttacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     6001 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     6061 tgggcgccag ggtggttttt cttttcacca gtgagacggg caacagctga ttgcccttca
     6121 ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa
     6181 aatcctgttt gatggtggtt aacggcggga tataacatga gctgtcttcg gtatcgtcgt
     6241 atcccactac cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg
     6301 cgcccagcgc catctgatcg ttggcaacca gcatcgcagt gggaacgatg ccctcattca
     6361 gcatttgcat ggtttgttga aaaccggaca tggcactcca gtcgccttcc cgttccgcta
     6421 tcggctgaat ttgattgcga gtgagatatt tatgccagcc agccagacgc agacgcgccg
     6481 agacagaact taatgggccc gctaacagcg cgatttgctg gtgacccaat gcgaccagat
     6541 gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat aatactgttg atgggtgtct
     6601 ggtcagagac atcaagaaat aacgccggaa cattagtgca ggcagcttcc acagcaatgg
     6661 catcctggtc atccagcgga tagttaatga tcagcccact gacgcgttgc gcgagaagat
     6721 tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc taccatcgac accaccacgc
     6781 tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca
     6841 gggccagact ggaggtggca acgccaatca gcaacgactg tttgcccgcc agttgttgtg
     6901 ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc ttccactttt tcccgcgttt
     6961 tcgcagaaac gtggctggcc tggttcacca cgcgggaaac ggtctgataa gagacaccgg
     7021 catactctgc gacatcgtat aacgttactg gtttcacatt caccaccctg aattgactct
     7081 cttccgggcg ctatcatgcc ataccgcgaa aggttttgcg ccattcgatg gtgtccggga
     7141 tctcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg
     7201 ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc
     7261 ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg
     7321 cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg
     7381 gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga
     7441 aat
//
