LOCUS       sequence-274606-       12144 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12144)
  AUTHORS   Isakoff SJ, Engelman JA, Irie HY, Luo J, Brachmann SM, Pearline RV, 
            Cantley LC, Brugge JS
  TITLE     Breast cancer-associated PIK3CA mutations are oncogenic in mammary 
            epithelial cells.
  JOURNAL   Cancer Res. 2005 Dec 1. 65(23):10992-1000.
  PUBMED    16322248
REFERENCE   2  (bases 1 to 12144)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer Res.
            2005 Dec 1. 65(23):10992-1000."
COMMENT     Sequence Label: JP1520 PIK3CA WT HA
FEATURES             Location/Qualifiers
     source          1..12144
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     581..605
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    complement(686..719)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      3940..3949
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4016..4042
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             4016..4042
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             4072..4107
                     /codon_start=1
                     /product="6x(His-Asn) affinity tag"
                     /label=6xHN
                     /translation="HNHNHNHNHNHN"
     terminator      complement(4143..4237)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(4258..4917)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     4832..4851
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     protein_bind    complement(4946..4979)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(6359..6375)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6359..6375)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6372..6394)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6383..6399
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6407..6413,6414..6431,6432..6437))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6452..6473
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6590..6607)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6761..7349)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6841..6860)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7520..8311,8312..8380))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8143..8162
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8381..8485)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8553..8571
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8609..8631)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8731..8750
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8944..8966
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8958..8975
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8959..8975
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    10181..10538
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             10603..11019
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     10949..10971
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     10987..11003
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    11029..11403
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     11341..11357
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     CDS             11406..12005
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MAEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     11902..11922
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      601 agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac cgggaccgat
      661 ccagcctccg cggccccaag cttcgataac ttcgtatagc atacattata cgaagttatc
      721 agtcgacggt accggacata tgcccgggaa ttcctgcagg atccgctcga gaagcttggt
      781 accgagctcg gatctcgaga tgcctccaag accatcatca ggtgaactgt ggggcatcca
      841 cttgatgccc ccaagaatcc tagtagaatg tttactacca aatgggatga tagtgacttt
      901 agaatgcctc cgtgaggcta cgttaataac gataaagcat gaactattta aagaagcaag
      961 aaaataccct ctccatcaac ttcttcaaga tgaatcttct tacattttcg taagtgttac
     1021 ccaagaagca gaaagggaag aattttttga tgaaacaaga cgactttgtg accttcggct
     1081 ttttcaaccc tttttaaaag taattgaacc agtaggcaac cgtgaagaaa agatcctcaa
     1141 tcgagaaatt ggttttgcta tcggcatgcc agtgtgtgaa ttcgatatgg ttaaagatcc
     1201 agaagtacag gacttccgaa gaaatattct caatgtttgt aaagaagctg tggatcttag
     1261 ggatcttaat tcacctcata gtagagcaat gtatgtttat cctccaaatg tagaatcttc
     1321 accagaactg ccaaagcaca tatataataa attggataaa gggcaaataa tagtggtgat
     1381 ttgggtaata gtttctccaa ataatgacaa acagaagtat actctgaaaa tcaaccatga
     1441 ctgtgtgcca gaacaagtaa ttgctgaagc aatcaggaaa aaaactcgaa gtatgttgct
     1501 atcatctgaa caactaaaac tctgtgtttt agaatatcag ggcaagtata ttttaaaagt
     1561 gtgtggatgt gatgaatact tcctagaaaa atatcctctg agtcagtata agtatataag
     1621 aagctgtata atgcttggga ggatgcccaa tttgatgctg atggctaaag aaagcctcta
     1681 ttctcaactg ccaatggact gttttacaat gccatcatat tccagacgca tctccacagc
     1741 tacgccatat atgaatggag aaacatctac aaaatccctt tgggttataa atagtgcact
     1801 cagaataaaa attctttgtg caacctatgt gaatgtaaat attcgagaca ttgacaagat
     1861 ttatgttcga acaggtatct accatggagg agaaccctta tgtgataatg tgaacactca
     1921 aagagtacct tgttccaatc ccaggtggaa tgaatggctg aattacgata tatacattcc
     1981 tgatcttcct cgtgctgctc gactttgcct ttccatttgt tctgttaaag gccgaaaggg
     2041 tgctaaagag gaacactgtc cattggcctg gggaaatata aacttgtttg attacacaga
     2101 tactctagta tctggaaaaa tggctttgaa tctttggcca gtacctcatg gactagaaga
     2161 tttgctgaac cctattggtg ttactggatc aaatccaaat aaagaaactc catgtttaga
     2221 gttggagttt gactggttca gcagtgtggt aaagtttcca gatatgtcag tgattgaaga
     2281 gcatgccaat tggtctgtat cccgtgaagc aggatttagt tattcccatg caggactgag
     2341 taacagacta gctagagaca atgaattaag agaaaatgat aaagaacagc tccgagcaat
     2401 ttgtacacga gatcctctat ctgaaatcac tgagcaagag aaagattttc tgtggagcca
     2461 cagacactat tgtgtaacta tccccgaaat tctacccaaa ttgcttctgt ctgttaaatg
     2521 gaactctaga gatgaagtag ctcagatgta ctgcttggta aaagattggc ctccaatcaa
     2581 gcctgaacag gctatggagc ttctggactg caattaccca gatcctatgg ttcgaggttt
     2641 tgctgttcgg tgcttagaaa aatatttaac agatgacaaa ctttctcagt acctaattca
     2701 gctagtacag gtactaaaat atgaacagta tttggataac ctgcttgtga gatttttact
     2761 caaaaaagcg ttaactaatc aaaggatcgg tcactttttc ttttggcatt taaaatctga
     2821 gatgcacaat aaaacagtta gtcagaggtt tggcctgctt ttggagtcct attgccgtgc
     2881 atgtgggatg tatctgaagc accttaatag gcaagttgag gctatggaaa agctcattaa
     2941 cttgactgac attctcaaac aagagaagaa ggatgaaaca caaaaggtac agatgaagtt
     3001 tttagttgag caaatgcggc gaccagattt catggatgct ctccagggct ttctgtctcc
     3061 tctaaaccct gctcatcagc tgggaaatct caggcttgaa gagtgtcgaa ttatgtcttc
     3121 tgcaaaaagg ccactgtggt tgaattggga gaacccagac atcatgtcag aattactctt
     3181 tcagaacaat gagatcatct ttaaaaatgg ggatgattta cggcaagata tgctaaccct
     3241 tcagattatt cgcattatgg aaaatatctg gcaaaatcaa ggtcttgatc ttcgaatgtt
     3301 accttatgga tgtctgtcaa tcggtgactg tgtgggactt atcgaggtgg tgagaaattc
     3361 tcacactata atgcagattc agtgtaaagg aggcctgaaa ggtgcactgc agtttaacag
     3421 ccacacactc catcagtggc tcaaagacaa gaacaagggg gaaatatatg atgcggccat
     3481 cgatttgttt acacgatcat gtgctggata ttgtgttgcc accttcattt tgggaattgg
     3541 agatcgtcac aatagtaata tcatggttaa agatgatgga caactgtttc atatagattt
     3601 tggacacttt ttggatcaca agaagaaaaa atttggttat aaacgagagc gcgtgccgtt
     3661 tgttttgaca caagatttct taatagtgat tagtaaagga gcccaagaat gcacaaagac
     3721 aagagaattt gagaggtttc aggagatgtg ttacaaggct tatctagcta ttcggcagca
     3781 tgccaatctc ttcataaatc ttttctcaat gatgcttggc tctggaatgc cagaactgca
     3841 atcttttgat gatattgcat acattcgaaa gaccctagct ttagataaaa ctgagcaaga
     3901 ggctttggag tatttcatga aacaaatgaa tgatgcacac catggtggct ggacaacaaa
     3961 aatggattgg atcttccaca caattaagca gcatgctttg aacctcgaga tggcctaccc
     4021 ctacgacgtg cccgactacg cctcctgatc tagagggccc aggtaagtgg tcataatcat
     4081 aatcataatc ataatcataa tcacaactag cctaggagat cctggtcatg actagtgctt
     4141 ggattctcac caataaaaaa cgcccggcgg caaccgagcg ttctgaacaa atccagatgg
     4201 agttctgagg tcattactgg atctatcaac aggagtccaa gcgagctcga tatcaaatta
     4261 cgccccgccc tgccactcat cgcagtactg ttgtaattca ttaagcattc tgccgacatg
     4321 gaagccatca caaacggcat gatgaacctg aatcgccagc ggcatcagca ccttgtcgcc
     4381 ttgcgtataa tatttgccca tggtgaaaac gggggcgaag aagttgtcca tattggccac
     4441 gtttaaatca aaactggtga aactcaccca gggattggct gagacgaaaa acatattctc
     4501 aataaaccct ttagggaaat aggccaggtt ttcaccgtaa cacgccacat cttgcgaata
     4561 tatgtgtaga aactgccgga aatcgtcgtg gtattcactc cagagcgatg aaaacgtttc
     4621 agtttgctca tggaaaacgg tgtaacaagg gtgaacacta tcccatatca ccagctcacc
     4681 gtctttcatt gccatacgaa attccggatg agcattcatc aggcgggcaa gaatgtgaat
     4741 aaaggccgga taaaacttgt gcttattttt ctttacggtc tttaaaaagg ccgtaatatc
     4801 cagctgaacg gtctggttat aggtacattg agcaactgac tgaaatgcct caaaatgttc
     4861 tttacgatgc cattgggata tatcaacggt ggtatatcca gtgatttttt tctccatttt
     4921 agcttcctta gctcctgaaa gatccataac ttcgtatagc atacattata cgaagttata
     4981 gatccaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     5041 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgcctcg
     5101 aggatccgga ttagtccaat ttgttaaaga caggatatca gtggtccagg ctctagtttt
     5161 gactcaacaa tatcaccagc tgaagcctat agagtacgag ccatagataa aataaaagat
     5221 tttatttagt ctccagaaaa aggggggaat gaaagacccc acctgtaggt ttggcaagct
     5281 agcttaagta acgccatttt gcaaggcatg gaaaatacat aactgagaat agagaagttc
     5341 agatcaaggt taggaacaga gagacagcag aatatgggcc aaacaggata tctgtggtaa
     5401 gcagttcctg ccccggctca gggccaagaa cagttggaac agcagaatat gggccaaaca
     5461 ggatatctgt ggtaagcagt tcctgccccg gctcagggcc aagaacagat ggtccccaga
     5521 tgcggtcccg ccctcagcag tttctagaga accatcagat gtttccaggg tgccccaagg
     5581 acctgaaatg accctgtgcc ttatttgaac taaccaatca gttcgcttct cgcttctgtt
     5641 cgcgcgcttc tgctccccga gctcaataaa agagcccaca acccctcact cggcgcgcca
     5701 gtcctccgat agactgcgtc gcccgggtac ccgtgttctc aataaaccct cttgcagttg
     5761 catccgactc gtggtctcgc tgttccttgg gagggtctcc tctgagtgat tgactacccg
     5821 tcagcggggg tctttcagtt tctcccacct acacaggtct cactaacatt cctgatgtgc
     5881 cgcagggact ccgtcagccc ggtttttgtt tataataaaa tgcaagaaca gtgttccctt
     5941 caagccagac tacatcctga ctctcggctt tataaaagaa tgttgaaggg ctctgtggac
     6001 tatctgccac acgactttta agatttttat gcctcctgga tgagggattt agtcaatcta
     6061 tcctcgtcta ttttgctggc ttctccgtat tttaaatttc tagtttgcac tcccttcctg
     6121 agagcacggc gattgcagag tagttaatac tctgagggca ggcttctgtg aaaaggttgc
     6181 ctgggctcag tgtgagattt tgccataaaa aggggtcctg cccctgtgta cagacagatc
     6241 ggaatctaga gtgcatactc agagtccccg cggttccggg gctctgatct cagggcatct
     6301 ttgcctagag atcctctacg ccggacgcat cgtggccctc gaattaattc gtaatcatgt
     6361 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     6421 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
     6481 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
     6541 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     6601 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     6661 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     6721 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     6781 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     6841 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     6901 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     6961 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     7021 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     7081 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     7141 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     7201 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     7261 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     7321 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     7381 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     7441 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     7501 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     7561 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     7621 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     7681 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     7741 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     7801 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     7861 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     7921 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     7981 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     8041 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     8101 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     8161 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     8221 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     8281 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     8341 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     8401 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     8461 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag
     8521 aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc
     8581 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
     8641 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
     8701 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
     8761 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
     8821 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
     8881 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
     8941 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gcttgcatgc ctgcaggtcg
     9001 actctaggca cataaagaaa aacataacta accaagctgc agccgagaca gtgaaaagaa
     9061 ccgttaaaac ggtttgtttt aaataaactg aattatttag agtcatttct ttggtaggaa
     9121 agtacattgg cacgtaaagg agcccaaagc aatctgtgga aagcccaggc tgggagccca
     9181 gcagtttgca tcccctcctg gcgtgtacct aagggtttct taattgtgtg gtttctaaat
     9241 cttccagagg gtttgtctca ttcacttcca cttcggtgca caatacttgg acgcggattt
     9301 actgtcttag catctatcgg tggccttcga ttgaggctga acctgaggcc cacttcttca
     9361 gcttgttaag gagagcacaa gcaccagaag aggctgaccc ggcagacctg tgggcatttt
     9421 taacaagggc ctcctgggtc tgtgggaggc aggcttacat aaggtgcaaa ttagaaatat
     9481 aaataataag cccatatcaa tttgtcatct ttttttaagc tcaagttttg aaagacccca
     9541 cctgtaggtt tggcaagcta gcttaagtaa cgccattttg caaggcatgg aaaatacata
     9601 actgagaata gagaagttca gatcaaggtt aggaacagag agacagcaga atatgggcca
     9661 aacaggatat ctgtggtaag cagttcctgc cccggctcag ggccaagaac agttggaaca
     9721 gcagaatatg ggccaaacag gatatctgtg gtaagcagtt cctgccccgg ctcagggcca
     9781 agaacagatg gtccccagat gcggtcccgc cctcagcagt ttctagagaa ccatcagatg
     9841 tttccagggt gccccaagga cctgaaatga ccctgtgcct tatttgaact aaccaatcag
     9901 ttcgcttctc gcttctgttc gcgcgcttct gctccccgag ctcaataaaa gagcccacaa
     9961 cccctcactc ggcgcgccag tcctccgata gactgcgtcg cccgggtacc cgtgttctca
    10021 ataaaccctc ttgcagttgc atccgactcg tggtctcgct gttccttggg agggtctcct
    10081 ctgagtgatt gactacccgt cagcggggtc tttcatttgg aggttccacc gagatttgga
    10141 gacccctgcc cagggaccac cgaccccccc gccgggaggt aagctggcca gcaacttatc
    10201 tgtgtctgtc cgattgtcta gtgtctatga ctgattttat gcgcctgcgt cggtactagt
    10261 tagctaacta gctctgtatc tggcggaccc gtggtggaac tgacgagttc ggaacacccg
    10321 gccgcaaccc tgggagacgt cccagggact tcgggggccg tttttgtggc ccgacctgag
    10381 tcctaaaatc ccgatcgttt aggactcttt ggtgcacccc ccttagagga gggatatgtg
    10441 gttctggtag gagacgagaa cctaaaacag ttcccgcctc cgtctgaatt tttgctttcg
    10501 gtttgggacc gaagccgcgc cgcgcgtctt gtctgctgca gcatcgttct gtgttgtctc
    10561 tgtctgactg tgtttctgta tttgtctgaa aatatgggcc cgggctagac tgttaccact
    10621 cccttaagtt tgaccttagg tcactggaaa gatgtcgagc ggatcgctca caaccagtcg
    10681 gtagatgtca agaagagacg ttgggttacc ttctgctctg cagaatggcc aacctttaac
    10741 gtcggatggc cgcgagacgg cacctttaac cgagacctca tcacccaggt taagatcaag
    10801 gtcttttcac ctggcccgca tggacaccca gaccaggtcc cctacatcgt gacctgggaa
    10861 gccttggctt ttgacccccc tccctgggtc aagccctttg tacaccctaa gcctccgcct
    10921 cctcttcctc catccgcccc gtctctcccc cttgaacctc ctcgttcgac cccgcctcga
    10981 tcctcccttt atccagccct cactccttct ctaggcgccc ccatatggcc atatgagatc
    11041 ttatatgggg cacccccgcc ccttgtaaac ttccctgacc ctgacatgac aagagttact
    11101 aacagcccct ctctccaagc tcacttacag gctctctact tagtccagca cgaagtctgg
    11161 agacctctgg cggcagccta ccaagaacaa ctggaccgac cggtggtacc tcacccttac
    11221 cgagtcggcg acacagtgtg ggtccgccga caccagacta agaacctaga acctcgctgg
    11281 aaaggacctt acacagtcct gctgaccacc cccaccgccc tcaaagtaga cggcatcgca
    11341 gcttggatac acgccgccca cgtgaaggct gccgaccccg ggggtggacc atcctctaga
    11401 ctgccatggc cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtcccccggg
    11461 ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgacc
    11521 cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc
    11581 tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc
    11641 cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga
    11701 gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca
    11761 aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc
    11821 tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct
    11881 tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca
    11941 ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg
    12001 cctgagcggc cgcccggcca ttagccatat tattcattgg ttatatagca taaatcaata
    12061 ttggctattg gccattgcat acgttgtatc catatcataa tatgtacatt tatattggct
    12121 catgtccaac attaccgcca tgtt
//
