LOCUS       sequence-274610-       12988 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  piggyBac expression vector with TET-ON system and Zeocin selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12988)
  TITLE     Minoru Ko Lab plasmids
REFERENCE   2  (bases 1 to 12988)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PB-TET-ZH-lox66FRT
FEATURES             Location/Qualifiers
     source          1..12988
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(28..456)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(138..159)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     350..369
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     598..614
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     621..640
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        621..639
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   706..768
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     1177..1193
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1204..1703
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(1239..1262)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     1636..1655
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             1723..2097
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     primer_bind     complement(2155..2174)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    2220..2275
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2274..2293)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(2932..2948)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2956..2972
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2980..2986,2987..3004,3005..3010))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3025..3046
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     protein_bind    3129..3147
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3166..3184
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3203..3221
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3240..3258
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3277..3295
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3314..3332
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3351..3369
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3388..3406
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     3428..3452
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    3532..3656
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(3681..3686,3687..3704,3705..3711)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             3765..4424
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(3831..3850)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             4766..5071
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     4950..4969
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(5112..5236)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_feature    5311..5863
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5455..5472)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5682..5701
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5902..8943
                     /codon_start=1
                     /gene="lacZ"
                     /product="beta-galactosidase"
                     /label=lacZ
                     /translation="VVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRS
                     LNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPP
                     FVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEF
                     DLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR
                     FNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRV
                     TLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKP
                     LLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGL
                     YVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGA
                     NHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLS
                     LPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNP
                     WSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYL
                     FRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQ
                     PNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQ
                     SGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEA
                     ALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHP
                     ARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLR
                     CGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDD
                     SWSPSVSAEFQLSAGRYHYQLVWCQ"
     CDS             8968..9771
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9031..9050)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9641..9660
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(9803..9820)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9809..10033
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   10472..10506
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     protein_bind    complement(10533..10566)
                     /label=lox66
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    10599..10646
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        complement(10798..10816)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(10837..10853)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    10861..10877
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10885..10891,10892..10909,10910..10915))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10930..10951
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11068..11085)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11239..11827)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11319..11338)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11998..12789,12790..12858))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12621..12640
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12859..12963)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat
       61 tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga
      121 tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca
      181 acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct
      241 aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc
      301 cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag
      361 cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca
      421 cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc aggctgcgca
      481 actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg
      541 gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta
      601 aaacgacggc cagtgaattg taatacgact cactataggg cgaattggag ctcggtattc
      661 acgacagcag gctgaataat aaaaaaatta gaaactatta tttaacccta gaaagataat
      721 catattgtga cgtacgttaa agataatcat gcgtaaaatt gacgcatgtg ttttatcggt
      781 ctgtatatcg aggtttattt attaatttga atagatatta agttttatta tatttacact
      841 tacatactaa taataaattc aacaaacaat ttatttatgt ttatttattt attaaaaaaa
      901 aacaaaaact caaaatttct tctataaagt aacaaaactt ttaaacattc tctcttttac
      961 aaaaataaac ttattttgta ctttaaaaac agtcatgttg tattataaaa taagtaatta
     1021 gcttaaccta tacataatag aaacaaatta tacttattag tcagtcagaa acaactttgg
     1081 cacatatcaa tattatgctc tcgttactgg cgaaaggggg atgtgctgca aggcgattaa
     1141 gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgaattct
     1201 accgggtagg ggaggcgctt ttcccaaggc agtctggagc atgcgcttta gcagccccgc
     1261 tgggcacttg gcgctacaca agtggcctct ggcctcgcac acattccaca tccaccggta
     1321 ggcgccaacc ggctccgttc tttggtggcc ccttcgcgcc accttctact cctcccctag
     1381 tcaggaagtt cccccccgcc ccgcagctcg cgtcgtgcag gacgtgacaa atggaagtag
     1441 cacgtctcac tagtctcgtg cagatggaca gcaccgctga gcaatggaag cgggtaggcc
     1501 tttggggcag cggccaatag cagctttgct ccttcgcttt ctgggctcag aggctgggaa
     1561 ggggtgggtc ccggggcggg ctcaggggcg ggctcagggg cggggcgggc gcccgaaggt
     1621 cctccggagg cccggcattc tgcacgcttc aaaagcgcac gtctgccgcg ctgttctcct
     1681 cttcctcatc tccgggcctt tcgaccgatc atcagatcta ccatggccaa gttgaccagt
     1741 gccgttccgg tgctcaccgc gcgcgacgtc gccggagcgg tcgagttctg gaccgaccgg
     1801 ctcgggttct cccgggactt cgtggaggac gacttcgccg gtgtggtccg ggacgacgtg
     1861 accctgttca tcagcgcggt ccaggaccag gtggtgccgg acaacaccct ggcctgggtg
     1921 tgggtgcgcg gcctggacga gctgtacgcc gagtggtcgg aggtcgtgtc cacgaacttc
     1981 cgggacgcct ccgggccggc catgaccgag atcggcgagc agccgtgggg gcgggagttc
     2041 gccctgcgcg acccggccgg caactgcgtg cacttcgtgg ccgaggagca ggactgaccg
     2101 acgccgacca acaccgccgg tccgacgcgg cccgacgggt ccgagggggg tcgatctttt
     2161 tccctctgcc aaaaattatg gggacatcat gaagcccctt gagcatctga cttctggcta
     2221 ataaaggaaa tttattttca ttgcaatagt gtgttggaat tttttgtgtc tctcactcgg
     2281 aaggacatat gggagggcaa atcatttaaa acatcagaat gagtatttgg tttagagttt
     2341 ggcaacatat gcccatatgc tggctgccat gaacaaaggt tggctataaa gaggtcatca
     2401 gtatatgaaa cagccccctg ctgtccattc cttattccat agaaaagcct tgacttgagg
     2461 ttagattttt tttatatttt gttttgtgtt atttttttct ttaacatccc taaaattttc
     2521 cttacatgtt ttactagcca gatttttcct cctctcctga ctactcccag tcatagctgt
     2581 ccctcttctc ttatggagat ccctcgacct gcagcccaag cttggcgtaa tcatggtcat
     2641 ttaaactcta gagagctcac ggggacagcc cccccccaaa gcccccaggg atgtaattac
     2701 gtccctcccc cgctaggggg cagcagcgag ccgcccgggg ctccgctccg gtccggcgct
     2761 ccccccgcat ccccgagccg gcagcgtgcg gggacagccc gggcacgggg aaggtggcac
     2821 gggatcgctt tcctctgaac gcttctcgct gctctttgag cctgcagaca cctgggggat
     2881 acggggaaaa agcttgtcga cctgcaggca tgcaagcttg gcgtaatcat ggtcatagct
     2941 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
     3001 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
     3061 actgcccgct ttccagtcgg gaaacctgtc gtgccagtaa ctcgagggat ccaagctggc
     3121 cgcacgtctc cctatcagtg atagagaagt cgacacgtct cgagctccct atcagtgata
     3181 gagaaggtac gtctagaacg tctccctatc agtgatagag aagtcgacac gtctcgagct
     3241 ccctatcagt gatagagaag gtacgtctag aacgtctccc tatcagtgat agagaagtcg
     3301 acacgtctcg agctccctat cagtgataga gaaggtacgt ctagaacgtc tccctatcag
     3361 tgatagagaa gtcgacacgt ctcgagctcc ctatcagtga tagagaaggt accccctata
     3421 taagcagagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg
     3481 acctccatag aagacaccgg gaccgatcca gcctccgcgg tggcggccat cacaagtttg
     3541 tacaaaaaag ctgaacgaga aacgtaaaat gatataaata tcaatatatt aaattagatt
     3601 ttgcataaaa aacagactac ataatactgt aaaacacaac atatccagtc actatggcgg
     3661 ccgcattagg caccccaggc tttacacttt atgcttccgg ctcgtataat gtgtggattt
     3721 tgagttagga tccgtcgaga ttttcaggag ctaaggaagc taaaatggag aaaaaaatca
     3781 ctggatatac caccgttgat atatcccaat ggcatcgtaa agaacatttt gaggcatttc
     3841 agtcagttgc tcaatgtacc tataaccaga ccgttcagct ggatattacg gcctttttaa
     3901 agaccgtaaa gaaaaataag cacaagtttt atccggcctt tattcacatt cttgcccgcc
     3961 tgatgaatgc tcatccggaa ttccgtatgg caatgaaaga cggtgagctg gtgatatggg
     4021 atagtgttca cccttgttac accgttttcc atgagcaaac tgaaacgttt tcatcgctct
     4081 ggagtgaata ccacgacgat ttccggcagt ttctacacat atattcgcaa gatgtggcgt
     4141 gttacggtga aaacctggcc tatttcccta aagggtttat tgagaatatg tttttcgtct
     4201 cagccaatcc ctgggtgagt ttcaccagtt ttgatttaaa cgtggccaat atggacaact
     4261 tcttcgcccc cgttttcacc atgggcaaat attatacgca aggcgacaag gtgctgatgc
     4321 cgctggcgat tcaggttcat catgccgttt gtgatggctt ccatgtcggc agaatgctta
     4381 atgaattaca acagtactgc gatgagtggc agggcggggc gtaaacgcgt ggatccggct
     4441 tactaaaagc cagataacag tatgcgtatt tgcgcgctga tttttgcggt ataagaatat
     4501 atactgatat gtatacccga agtatgtcaa aaagaggtat gctatgaagc agcgtattac
     4561 agtgacagtt gacagcgaca gctatcagtt gctcaaggca tatatgatgt caatatctcc
     4621 ggtctggtaa gcacaaccat gcagaatgaa gcccgtcgtc tgcgtgccga acgctggaaa
     4681 gcggaaaatc aggaagggat ggctgaggtc gcccggttta ttgaaatgaa cggctctttt
     4741 gctgacgaga acaggggctg gtgaaatgca gtttaaggtt tacacctata aaagagagag
     4801 ccgttatcgt ctgtttgtgg atgtacagag tgatattatt gacacgcccg ggcgacggat
     4861 ggtgatcccc ctggccagtg cacgtctgct gtcagataaa gtctcccgtg aactttaccc
     4921 ggtggtgcat atcggggatg aaagctggcg catgatgacc accgatatgg ccagtgtgcc
     4981 ggtctccgtt atcggggaag aagtggctga tctcagccac cgcgaaaatg acatcaaaaa
     5041 cgccattaac ctgatgttct ggggaatata aatgtcaggc tcccttatac acagccagtc
     5101 tgcaggtcga ccatagtgac tggatatgtt gtgttttaca gtattatgta gtctgttttt
     5161 tatgcaaaat ctaatttaat atattgatat ttatatcatt ttacgtttct cgttcagctt
     5221 tcttgtacaa agtggtgatg gccgctctag ctagtcgata agctaattcc kccccccccc
     5281 cccccccccc cycycccycc ccccccccta acgttactgg ccgaagccgc ttggaataag
     5341 gccggtgtgc gtttgtctat atgttatttt ccaccatatt gccgtctttt ggcaatgtga
     5401 gggcccggaa acctggccct gtcttcttga cgagcattcc taggggtctt tcccctctcg
     5461 ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt
     5521 gaagacaaac aacgtctgta gcgacccttt gcaggcagcg gaacccccca cctggcgaca
     5581 ggtgcctctg cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc
     5641 agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa atggctctcc tcaagcgtat
     5701 tcaacaaggg gctgaaggat gcccagaagg taccccattg tatgggatct gatctggggc
     5761 ctcggtgcac atgctttaca tgtgtttagt cgaggttaaa aaaacgtcta ggccccccga
     5821 accacgggga cgtggttttc ctttgaaaaa cacgatgata atatggccat gagcttgatg
     5881 atctgtgaca tggcggatcc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
     5941 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
     6001 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg gcgctttgcc
     6061 tggtttccgg caccagaagc ggtgccggaa agctggctgg agtgcgatct tcctgaggcc
     6121 gatactgtcg tcgtcccctc aaactggcag atgcacggtt acgatgcgcc catctacacc
     6181 aacgtgacct atcccattac ggtcaatccg ccgtttgttc ccacggagaa tccgacgggt
     6241 tgttactcgc tcacatttaa tgttgatgaa agctggctac aggaaggcca gacgcgaatt
     6301 atttttgatg gcgttaactc ggcgtttcat ctgtggtgca acgggcgctg ggtcggttac
     6361 ggccaggaca gtcgtttgcc gtctgaattt gacctgagcg catttttacg cgccggagaa
     6421 aaccgcctcg cggtgatggt gctgcgctgg agtgacggca gttatctgga agatcaggat
     6481 atgtggcgga tgagcggcat tttccgtgac gtctcgttgc tgcataaacc gactacacaa
     6541 atcagcgatt tccatgttgc cactcgcttt aatgatgatt tcagccgcgc tgtactggag
     6601 gctgaagttc agatgtgcgg cgagttgcgt gactacctac gggtaacagt ttctttatgg
     6661 cagggtgaaa cgcaggtcgc cagcggcacc gcgcctttcg gcggtgaaat tatcgatgag
     6721 cgtggtggtt atgccgatcg cgtcacacta cgtctgaacg tcgaaaaccc gaaactgtgg
     6781 agcgccgaaa tcccgaatct ctatcgtgcg gtggttgaac tgcacaccgc cgacggcacg
     6841 ctgattgaag cagaagcctg cgatgtcggt ttccgcgagg tgcggattga aaatggtctg
     6901 ctgctgctga acggcaagcc gttgctgatt cgaggcgtta accgtcacga gcatcatcct
     6961 ctgcatggtc aggtcatgga tgagcagacg atggtgcagg atatcctgct gatgaagcag
     7021 aacaacttta acgccgtgcg ctgttcgcat tatccgaacc atccgctgtg gtacacgctg
     7081 tgcgaccgct acggcctgta tgtggtggat gaagccaata ttgaaaccca cggcatggtg
     7141 ccaatgaatc gtctgaccga tgatccgcgc tggctaccgg cgatgagcga acgcgtaacg
     7201 cgaatggtgc agcgcgatcg taatcacccg agtgtgatca tctggtcgct ggggaatgaa
     7261 tcaggccacg gcgctaatca cgacgcgctg tatcgctgga tcaaatctgt cgatccttcc
     7321 cgcccggtgc agtatgaagg cggcggagcc gacaccacgg ccaccgatat tatttgcccg
     7381 atgtacgcgc gcgtggatga agaccagccc ttcccggctg tgccgaaatg gtccatcaaa
     7441 aaatggcttt cgctacctgg agagacgcgc ccgctgatcc tttgcgaata cgcccacgcg
     7501 atgggtaaca gtcttggcgg tttcgctaaa tactggcagg cgtttcgtca gtatccccgt
     7561 ttacagggcg gcttcgtctg ggactgggtg gatcagtcgc tgattaaata tgatgaaaac
     7621 ggcaacccgt ggtcggctta cggcggtgat tttggcgata cgccgaacga tcgccagttc
     7681 tgtatgaacg gtctggtctt tgccgaccgc acgccgcatc cagcgctgac ggaagcaaaa
     7741 caccagcagc agtttttcca gttccgttta tccgggcaaa ccatcgaagt gaccagcgaa
     7801 tacctgttcc gtcatagcga taacgagctc ctgcactgga tggtggcgct ggatggtaag
     7861 ccgctggcaa gcggtgaagt gcctctggat gtcgctccac aaggtaaaca gttgattgaa
     7921 ctgcctgaac taccgcagcc ggagagcgcc gggcaactct ggctcacagt acgcgtagtg
     7981 caaccgaacg cgaccgcatg gtcagaagcc gggcacatca gcgcctggca gcagtggcgt
     8041 ctggcggaaa acctcagtgt gacgctcccc gccgcgtccc acgccatccc gcatctgacc
     8101 accagcgaaa tggatttttg catcgagctg ggtaataagc gttggcaatt taaccgccag
     8161 tcaggctttc tttcacagat gtggattggc gataaaaaac aactgctgac gccgctgcgc
     8221 gatcagttca cccgtgcacc gctggataac gacattggcg taagtgaagc gacccgcatt
     8281 gaccctaacg cctgggtcga acgctggaag gcggcgggcc attaccaggc cgaagcagcg
     8341 ttgttgcagt gcacggcaga tacacttgct gatgcggtgc tgattacgac cgctcacgcg
     8401 tggcagcatc aggggaaaac cttatttatc agccggaaaa cctaccggat tgatggtagt
     8461 ggtcaaatgg cgattaccgt tgatgttgaa gtggcgagcg atacaccgca tccggcgcgg
     8521 attggcctga actgccagct ggcgcaggta gcagagcggg taaactggct cggattaggg
     8581 ccgcaagaaa actatcccga ccgccttact gccgcctgtt ttgaccgctg ggatctgcca
     8641 ttgtcagaca tgtatacccc gtacgtcttc ccgagcgaaa acggtctgcg ctgcgggacg
     8701 cgcgaattga attatggccc acaccagtgg cgcggcgact tccagttcaa catcagccgc
     8761 tacagtcaac agcaactgat ggaaaccagc catcgccatc tgctgcacgc ggaagaaggc
     8821 acatggctga atatcgacgg tttccatatg gggattggtg gcgacgactc ctggagcccg
     8881 tcagtatcgg cggaattcca gctgagcgcc ggtcgctacc attaccagtt ggtctggtgt
     8941 caggggatcc cccgggctgc agccaatatg ggatcggcca ttgaacaaga tggattgcac
     9001 gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca
     9061 atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt
     9121 gtcaagaccg acctgtccgg tgccctgaat gaactgcagg acgaggcagc gcggctatcg
     9181 tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga
     9241 agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct
     9301 cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg
     9361 gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg
     9421 gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc
     9481 gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat
     9541 ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac
     9601 tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt
     9661 gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct
     9721 cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg aggggatcaa
     9781 ttctctagag ctcgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt
     9841 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
     9901 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg
     9961 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg
    10021 gtgggctcta tggcttctga ggcggaaaga accagctggg gctcgatcct ctagagtcga
    10081 gggctgcagg aattcgatat caagctctag ccaacaagct cgtcatcgct ttgcagaaga
    10141 gcagagagga tatgctcatc gtctaaagaa ctacccattt tattatatat tagtcacgat
    10201 atctataaca agaaaatata tatataataa gttatcacgt aagtagaaca tgaaataaca
    10261 atataattat cgtatgagtt aaatcttaaa agtcacgtaa aagataatca tgcgtcattt
    10321 tgactcacgc ggtcgttata gttcaaaatc agtgacactt accgcattga caagcacgcc
    10381 tcacgggagc tccaagcggc gactgagatg tcctaaatgc acagcgacgg attcgcgcta
    10441 tttagaaaga gagagcaata tttcaagaat gcatgcgtca attttacgca gactatcttt
    10501 ctagggttaa atttgggcgg tcgagggacc taataacttc gtatagcata cattatacga
    10561 acggtaatta agggttccgg atccccatca agctaattga agttcctatt ccgaagttcc
    10621 tattctctag aaagtatagg aacttccagc tggaaatgat ttgcgcttta ctcgacctaa
    10681 actttaaaca cgtcatagaa tcttcgtttg acaaaaacca cattgtggcc aagctgtgtg
    10741 acgcgacgcg cgctaaagaa tggcaaacca agtcgcgcga ggtaccagct tttgttccct
    10801 ttagtgaggg ttaatttcga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
    10861 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
    10921 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
    10981 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
    11041 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
    11101 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
    11161 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
    11221 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
    11281 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
    11341 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
    11401 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
    11461 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
    11521 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
    11581 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
    11641 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
    11701 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
    11761 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
    11821 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
    11881 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
    11941 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
    12001 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
    12061 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
    12121 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
    12181 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
    12241 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
    12301 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
    12361 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
    12421 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
    12481 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
    12541 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
    12601 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
    12661 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
    12721 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
    12781 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
    12841 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
    12901 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
    12961 gcgcacattt ccccgaaaag tgccacct
//
