LOCUS       sequence-274613-        9741 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9741)
  AUTHORS   Vierbuchen T, Ostermeier A, Pang ZP, Kokubu Y, Sudhof TC, Wernig M
  TITLE     Direct conversion of fibroblasts to functional neurons by defined 
            factors.
  JOURNAL   Nature. 2010 Feb 25. 463(7284):1035-41.
  PUBMED    20107439
REFERENCE   2  (bases 1 to 9741)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2010 Feb 25. 463(7284):1035-41."
COMMENT     Sequence Label: Tet-O-FUW-Zic1
FEATURES             Location/Qualifiers
     source          1..9741
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(46..65)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        237..616
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        618..816
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             834..1014
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1061..1186
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1679..1912
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2097..2141
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2439..2556
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(2613..2631,2632..2654,2655..2673,2674..2696,2697..2715,
                     2716..2738,2739..2757,2758..2780,2781..2799,2800..2822,
                     2823..2841,2842..2864,2865..2883)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2916..2954
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2951..2975
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     misc_feature    4432..5020
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4485..4505)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4903..4914)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5023..5039)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5024..5040)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5545..5725
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5751..5768)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5757..5981
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6027..6455
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6114..6133)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6324..6345
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6464..6484)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6469..6798
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6649..6784
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6711..6730
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6846..6893
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6912..7286
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7416..7537
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7453..7472)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7507..7526
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7586..7602)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7586..7602)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7599..7621)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7610..7626
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7634..7640,7641..7658,7659..7664))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7679..7700
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7817..7834)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7988..8576)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8068..8087)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8747..9538,9539..9607))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9370..9389
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9608..9712)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga
       61 tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg
      121 cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct
      181 gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca
      241 ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc atagcccata
      301 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
      361 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
      421 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
      481 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
      541 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
      601 catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt
      661 tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca
      721 ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg
      781 cggtaggcgt gtacggtggg aggtctatat aagcagcgcg ttttgcctgt actgggtctc
      841 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      901 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      961 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
     1021 cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg
     1081 gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat
     1141 tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg
     1201 gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa
     1261 attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct
     1321 gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac
     1381 aggatcagaa gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca
     1441 aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa
     1501 aagtaagacc accgcacagc aagcggccgc tgatcttcag acctggagga ggagatatga
     1561 gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag
     1621 tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag
     1681 gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga
     1741 cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc
     1801 tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc
     1861 tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt
     1921 ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta
     1981 ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta
     2041 acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga
     2101 atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa
     2161 caaattggct gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa
     2221 gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat
     2281 cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag
     2341 aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatcg gcactgcgtg
     2401 cgccaattct gcagacaaat ggcagtattc atccacaatt ttaaaagaaa aggggggatt
     2461 ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa
     2521 gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag ggacagcaga
     2581 gatccagttt ggttagatct cgagtttacc actccctatc agtgatagag aaaagtgaaa
     2641 gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt taccactccc
     2701 tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg atagagaaaa
     2761 gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc
     2821 actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag
     2881 agaaaagtga aagtcgagct cggtacccgg gtcgaggtag gcgtgtacgg tgggaggcct
     2941 atataagcag agctcgttta gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt
     3001 ttgacctcca tagaagacac cgggaccgat ccagcctccg cggccccgaa ttcgctagcc
     3061 accatgctcc tggacgccgg accccagtat cccgcgattg gtgtgaccac ctttggtgca
     3121 tcccggcacc actcggcggg cgacgtggcc gagagagacg tgggcctggg tatcaacccg
     3181 ttcgccgacg gcatgggcgc cttcaagctc aaccccagtt cgcacgaact ggcctcggct
     3241 ggccagacag ccttcacgtc gcaggctccg ggctacgccg ctgctgcggc cctgggccac
     3301 catcaccacc ccggccacgt cggctcctac tccagcgctg ctttcaattc tacccgggac
     3361 tttctgttcc gcaaccgagg cttcggcgac gcggcggcgg cagctagcgc ccagcacagt
     3421 ctcttcgctg cttcggccgg cggctttggg ggcccacacg gccatacgga cgccgcgggc
     3481 cacctccttt tttctgggct tcacgagcag gcggctggcc acgcttcgcc caacgtggtt
     3541 aacgggcaga tgcggctagg tttctccggg gacatgtacc cacggccgga acagtacggc
     3601 caggtgacca gcccgcgatc cgagcactat gctgccccgc agcttcacgg ctatgggccc
     3661 atgaacgtga acatggctgc acatcacggg gctggagcct tcttccgcta tatgcgccaa
     3721 cccatcaagc aagagcttat ctgtaaatgg atcgagccgg agcagctggc caaccccaaa
     3781 aagtcgtgca acaaaacttt cagcaccatg cacgagctgg tcacgcacgt cacggtggag
     3841 cacgtcggcg gcccagagca gagcaaccac atctgcttct gggaggagtg tccacgcgag
     3901 ggcaagccct tcaaagccaa atacaaactg gtcaaccaca tccgagtgca cacaggcgaa
     3961 aagccttttc cctgcccgtt tcctggctgc ggcaaggttt tcgcgcgttc agagaacctc
     4021 aagatccaca aaaggacaca cacaggggag aagcccttca agtgcgagtt cgagggctgc
     4081 gaccggcgct tcgccaacag cagcgaccgc aagaagcaca tgcacgtgca cacgagcgac
     4141 aagccctacc tttgcaagat gtgcgataag tcctacacgc accccagctc tctgcgtaaa
     4201 cacatgaagg tccatgagtc ctcttctcag ggctcacagc cttcgcccgc cgccagctcg
     4261 gggtatgagt cgtccacgcc acccaccatc gtgtctccca caacagacaa cccgaccacc
     4321 agctccatgt cgccctcctc ctccgcggtt caccacacag ccggccacag cgcgctctct
     4381 tccaatttta acgaatggta cgtttaagaa ttcgatatca agcttatcga taatcaacct
     4441 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
     4501 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
     4561 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
     4621 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
     4681 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
     4741 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
     4801 gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt
     4861 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
     4921 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
     4981 cctcagacga gtcggatctc cctttgggcc gcctccccgc atcgataccg tcgacctcga
     5041 gacctagaaa aacatggagc aatcacaagt agcaatacag cagctaccaa tgctgattgt
     5101 gcctggctag aagcacaaga ggaggaggag gtgggttttc cagtcacacc tcaggtacct
     5161 ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg
     5221 ggactggaag ggctaattca ctcccaacga agacaagata tccttgatct gtggatctac
     5281 cacacacaag gctacttccc tgattggcag aactacacac cagggccagg gatcagatat
     5341 ccactgacct ttggatggtg ctacaagcta gtaccagttg agcaagagaa ggtagaagaa
     5401 gccaatgaag gagagaacac ccgcttgtta caccctgtga gcctgcatgg gatggatgac
     5461 ccggagagag aagtattaga gtggaggttt gacagccgcc tagcatttca tcacatggcc
     5521 cgagagctgc atccggactg tactgggtct ctctggttag accagatctg agcctgggag
     5581 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     5641 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     5701 tagtcagtgt ggaaaatctc tagcagggcc cgtttaaacc cgctgatcag cctcgactgt
     5761 gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga
     5821 aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag
     5881 taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga
     5941 agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac
     6001 cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg
     6061 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     6121 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     6181 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     6241 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     6301 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
     6361 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
     6421 aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt gtgtcagtta
     6481 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     6541 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     6601 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
     6661 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     6721 gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     6781 ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcag
     6841 cacgtgttga caattaatca tcggcatagt atatcggcat agtataatac gacaaggtga
     6901 ggaactaaac catggccaag ttgaccagtg ccgttccggt gctcaccgcg cgcgacgtcg
     6961 ccggagcggt cgagttctgg accgaccggc tcgggttctc ccgggacttc gtggaggacg
     7021 acttcgccgg tgtggtccgg gacgacgtga ccctgttcat cagcgcggtc caggaccagg
     7081 tggtgccgga caacaccctg gcctgggtgt gggtgcgcgg cctggacgag ctgtacgccg
     7141 agtggtcgga ggtcgtgtcc acgaacttcc gggacgcctc cgggccggcc atgaccgaga
     7201 tcggcgagca gccgtggggg cgggagttcg ccctgcgcga cccggccggc aactgcgtgc
     7261 acttcgtggc cgaggagcag gactgacacg tgctacgaga tttcgattcc accgccgcct
     7321 tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc
     7381 gcggggatct catgctggag ttcttcgccc accccaactt gtttattgca gcttataatg
     7441 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     7501 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtata ccgtcgacct
     7561 ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     7621 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     7681 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     7741 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     7801 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     7861 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     7921 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     7981 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     8041 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     8101 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     8161 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     8221 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     8281 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     8341 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     8401 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     8461 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     8521 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     8581 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     8641 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     8701 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     8761 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     8821 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     8881 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     8941 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     9001 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     9061 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     9121 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     9181 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     9241 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     9301 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     9361 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     9421 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     9481 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     9541 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     9601 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     9661 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     9721 cccgaaaagt gccacctgac g
//
