LOCUS       sequence-274629-       12042 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Temperature-sensitive plasmid carries tnsABCD genes that encode 
            transposase biochemical machinery under an arabinose-inducible pBAD 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12042)
  AUTHORS   Jasinska W, Manhart M, Lerner J, Gauthier L, Serohijos AWR, 
            Bershtein S
  TITLE     Chromosomal barcoding of E. coli populations reveals lineage 
            diversity dynamics at high resolution.
  JOURNAL   Nat Ecol Evol. 2020 Feb 24. pii: 10.1038/s41559-020-1103-z. doi: 
            10.1038/s41559-020-1103-z.
  PUBMED    32094541
REFERENCE   2  (bases 1 to 12042)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Ecol 
            Evol. 2020 Feb 24. pii: 10.1038/s41559-020-1103-z. doi: 
            10.1038/s41559-020-1103-z."
COMMENT     Sequence Label: Tn7 helper plasmid
FEATURES             Location/Qualifiers
     source          1..12042
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(724..1602)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        1629..1913
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     1836..1855
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     primer_bind     complement(8604..8621)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      8774..8860
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      8952..8979
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        8998..9089
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9090..9158,9159..9950)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9308..9327)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     primer_bind     complement(10079..10098)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     oriT            10121..10230
                     /note="incP origin of transfer"
     primer_bind     complement(10268..10287)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     rep_origin      10970..11192
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             join(11240..12042,1..148)
                     /codon_start=1
                     /gene="rep101 encoding an A56V mutation"
                     /product="temperature-sensitive version of the RepA protein
                     needed for replication with the pSC101 origin (Armstrong et
                     al., 1984)"
                     /label=Rep101(Ts)
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYVQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
ORIGIN
        1 aactgcaaaa attcagctca ccagttttga ggcaaaattt ttgagtgaca tgcaaagtaa
       61 gtatgatctc aatggttcgt tctcatggct cacgcaaaaa caacgaacca cactagagaa
      121 catactggct aaatacggaa ggatctgagg ttcttatggc tcttgtatct atcagtgaag
      181 catcaagact aacaaacaaa agtagaacaa ctgttcaccg ttacatatca aagggaaaac
      241 tgtccatgca cagatgaaaa cggtgtaaaa aagatagata catcagagct tttacgagtt
      301 tttggtgcat ttaaagctgt tcaccatgaa cagatcgaca atgtaacaga tgaacagcat
      361 gtaacaccta atagaacagg tgaaaccagt aaaacaaagc aactagaaca tgaaattgaa
      421 cacctgagac aacttgttac agctcaacag tcacacatag acagcctgaa acaggcgatg
      481 ctgcttatcg aatcaaagct gccgacaaca cgggagccag tgacgcctcc cgtggggaaa
      541 aaatcatggc aattctggaa gaaatagcgc tttcagccgg ccacgatgcg tccggcgtag
      601 aggatctgct catgtttgac agcttatcat cgatgcataa tgtgcctgtc aaatggacga
      661 agcagggatt ctgcaaaccc tatgctactc cgtcaagccg tcaattgtct gattcgttac
      721 caattatgac aacttgacgg ctacatcatt cactttttct tcacaaccgg cacggaactc
      781 gctcgggctg gccccggtgc attttttaaa tacccgcgag aaatagagtt gatcgtcaaa
      841 accaacattg cgaccgacgg tggcgatagg catccgggtg gtgctcaaaa gcagcttcgc
      901 ctggctgata cgttggtcct cgcgccagct taagacgcta atccctaact gctggcggaa
      961 aagatgtgac agacgcgacg gcgacaagca aacatgctgt gcgacgctgg cgatatcaaa
     1021 attgctgtct gccaggtgat cgctgatgta ctgacaagcc tcgcgtaccc gattatccat
     1081 cggtggatgg agcgactcgt taatcgcttc catgcgccgc agtaacaatt gctcaagcag
     1141 atttatcgcc agcagctccg aatagcgccc ttccccttgc ccggcgttaa tgatttgccc
     1201 aaacaggtcg ctgaaatgcg gctggtgcgc ttcatccggg cgaaagaacc ccgtattggc
     1261 aaatattgac ggccagttaa gccattcatg ccagtaggcg cgcggacgaa agtaaaccca
     1321 ctggtgatac cattcgcgag cctccggatg acgaccgtag tgatgaatct ctcctggcgg
     1381 gaacagcaaa atatcacccg gtcggcaaac aaattctcgt ccctgatttt tcaccacccc
     1441 ctgaccgcga atggtgagat tgagaatata acctttcatt cccagcggtc ggtcgataaa
     1501 aaaatcgaga taaccgttgg cctcaatcgg cgttaaaccc gccaccagat gggcattaaa
     1561 cgagtatccc ggcagcaggg gatcattttg cgcttcagcc atacttttca tactcccgcc
     1621 attcagagaa gaaaccaatt gtccatattg catcagacat tgccgtcact gcgtctttta
     1681 ctggctcttc tcgctaacca aaccggtaac cccgcttatt aaaagcattc tgtaacaaag
     1741 cgggaccaaa gccatgacaa aaacgcgtaa caaaagtgtc tataatcacg gcagaaaagt
     1801 ccacattgat tatttgcacg gcgtcacact ttgctatgcc atagcatttt tatccataag
     1861 attagcggat cctacctgac gctttttatc gcaactctct actgtttctc catacccgtt
     1921 tttttgggct agcgaattcg agctcggtac ccaagtctta aactagacag aatagttgta
     1981 aactgaaatc agtccagtta tgctgtgaaa aagcatactg gacttttgtt atggctaaag
     2041 caaactcttc attttctgaa gtgcaaattg cccgtcgtat taaagagggg cgtggccaag
     2101 ggcatggtaa agactatatt ccatggctaa cagtacaaga agttccttct tcaggtcgtt
     2161 cccaccgtat ttattctcat aagacgggac gagtccatca tttgctatct gacttagagc
     2221 ttgctgtttt tctcagtctt gagtgggaga gcagcgtgct agatatacgc gagcagttcc
     2281 ccttattacc tagtgatacc aggcagattg caatagatag tggtattaag catcctgtta
     2341 ttcgtggtgt agatcaggtt atgtctactg attttttagt ggactgcaaa gatggtcctt
     2401 ttgagcagtt tgctattcaa gtcaaacctg cagcagcctt acaagacgag cgtaccttag
     2461 aaaaactaga actagagcgt cgctattggc agcaaaagca aattccttgg ttcattttta
     2521 ctgataaaga aataaatccc gtagtaaaag aaaatattga atggctttat tcagtgaaaa
     2581 cagaagaagt ttctgcggag cttttagcac aactatcccc attggcccat atcctgcaag
     2641 aaaaaggaga tgaaaacatt atcaatgtct gtaagcaggt tgatattgct tatgatttgg
     2701 agttaggcaa aacattgagt gagatacgag ccttaaccgc aaatggtttt attaagttca
     2761 atatttataa gtctttcagg gcaaataagt gtgcagatct ctgtattagc caagtagtga
     2821 atatggagga gttgcgctat gtggcaaatt aatgaggttg tgctatttga taatgatccg
     2881 tatcgcattt tggctataga ggatggccaa gttgtctgga tgcaaataag cgctgataaa
     2941 ggagttccac aagctagggc tgagttgttg ctaatgcagt atttagatga aggccgctta
     3001 gttagaactg atgaccctta tgtacatctt gatttagaag agccgtctgt agattctgtc
     3061 agcttccaga agcgcgagga ggattatcga aaaattcttc ctattattaa tagtaaggat
     3121 cgtttcgacc ctaaagtcag aagcgaactc gttgagcatg tggtccaaga acataaggtt
     3181 actaaggcta cagtttataa gttgttacgc cgttactggc agcgtggtca aacgcctaat
     3241 gcattaattc ctgactacaa aaacagcggt gcaccagggg aaagacgttc agcgacagga
     3301 acagcaaaga ttggccgagc cagagaatat ggtaagggtg aaggaaccaa ggtaacgccc
     3361 gagattgaac gcctttttag gttgaccata gaaaagcacc tgttaaatca aaaaggtaca
     3421 aagaccaccg ttgcctatag acgatttgtg gacttgtttg ctcagtattt tcctcgcatt
     3481 ccccaagagg attacccaac actacgtcag tttcgttatt tttatgatcg agaataccct
     3541 aaagctcagc gcttaaagtc tagagttaaa gcaggggtat ataaaaaaga cgtacgaccc
     3601 ttaagtagta cagccacttc tcaggcgtta ggccctggga gtcgttatga gattgatgcc
     3661 acgattgctg atatttattt agtggatcat catgatcgcc aaaaaatcat aggaagacca
     3721 acgctttaca ttgtgattga tgtgtttagt cggatgatca cgggctttta tatcggcttt
     3781 gaaaatccgt cttatgtggt ggcgatgcag gcttttgtaa atgcttgctc tgacaaaacg
     3841 gccatttgtg cccagcatga tattgagatt agtagctcag actggccgtg tgtaggtttg
     3901 ccagatgtgt tgctagcgga ccgtggcgaa ttaatgagtc atcaggtcga agccttagtt
     3961 tctagtttta atgtgcgagt ggaaagtgct ccacctagac gtggcgatgc taaaggcata
     4021 gtggaaagca cttttagaac actacaagcc gagtttaagt cctttgcacc tggcattgta
     4081 gagggcagtc ggatcaaaag ccatggtgaa acagactata ggttagatgc atctctgtcg
     4141 gtatttgagt tcacacaaat tattttgcgt acgatcttat tcagaaataa ccatctggtg
     4201 atggataaat acgatcgaga tgctgatttt cctacagatt taccgtctat tcctgtccag
     4261 ctatggcaat ggggtatgca gcatcgtaca ggtagtttaa gggctgtgga gcaagagcag
     4321 ttgcgagtag cgttactgcc tcgccgaaag gtctctattt cttcatttgg cgttaatttg
     4381 tggggtttgt attactcggg gtcagagatt ctgcgtgagg gttggttgca gcggagcact
     4441 gatatagcta gacctcaaca tttagaagcg gcttatgacc cagtgctggt tgatacgatt
     4501 tatttgtttc cgcaagttgg cagccgtgta ttttggcgct gtaatctgac ggaacgtagt
     4561 cggcagttta aaggtctctc attttgggag gtttgggata tacaagcaca agaaaaacac
     4621 aataaagcca atgcgaagca ggatgagtta actaaacgca gggagcttga ggcgtttatt
     4681 cagcaaacca ttcagaaagc gaataagtta acgcccagta ctactgagcc caaatcaaca
     4741 cgcattaagc agattaaaac taataaaaaa gaagccgtga cctcggagcg taaaaaacgt
     4801 gcggagcatt tgaagccaag ctcttcaggt gatgaggcta aagttattcc tttcaacgca
     4861 gtggaagcgg atgatcaaga agattacagc ctacccacat acgtgcctga attatttcag
     4921 gatccaccag aaaaggatga gtcatgagtg ctacccggat tcaagcagtt tatcgtgata
     4981 cgggggtaga ggcttatcgt gataatcctt ttatcgaggc cttaccacca ttacaagagt
     5041 cagtgaatag tgctgcatca ctgaaatcct ctttacagct tacttcctct gacttgcaaa
     5101 agtcccgtgt tatcagagct cataccattt gtcgtattcc agatgactat tttcagccat
     5161 taggtacgca tttgctacta agtgagcgta tttcggtcat gattcgaggt ggctacgtag
     5221 gcagaaatcc taaaacagga gatttacaaa agcatttaca aaatggttat gagcgtgttc
     5281 aaacgggaga gttggagaca tttcgctttg aggaggcacg atctacggca caaagcttat
     5341 tgttaattgg ttgttctggt agtgggaaga cgacctctct tcatcgtatt ctagccacgt
     5401 atcctcaggt gatttaccat cgtgaactca atgtagagca ggtggtgtat ttgaaaatag
     5461 actgctcgca taatggttcg ctaaaagaaa tctgcttgaa ttttttcaga gcgttggatc
     5521 gagccttggg ctcgaactat gagcgtcgtt atggcttaaa acgtcatggt atagaaacca
     5581 tgttggcttt gatgtcgcaa atagccaatg cacatgcttt agggttgttg gttattgatg
     5641 aaattcagca tttaagccgc tctcgttcgg gtggatctca agagatgctg aacttttttg
     5701 tgacgatggt gaatattatt ggcgtaccag tgatgttgat tggtacccct aaagcacgag
     5761 agatttttga ggctgatttg cggtctgcac gtagaggggc agggtttgga gctatattct
     5821 gggatcctat acaacaaacg caacgtggaa agcccaatca agagtggatc gcttttacgg
     5881 ataatctttg gcaattacag cttttacaac gcaaagatgc gctgttatcg gatgaggtcc
     5941 gtgatgtgtg gtatgagcta agccaaggag tgatggacat tgtagtaaaa ctttttgtac
     6001 tcgctcagct ccgtgcgcta gctttaggca atgagcgtat taccgctggt ttattgcggc
     6061 aagtgtatca agatgagtta aagcctgtgc accccatgct agaggcatta cgctcgggta
     6121 tcccagaacg cattgctcgt tattctgatc tagtcgttcc cgagattgat aaacggttaa
     6181 tccaacttca gctagatatc gcagcgatac aagaacaaac accagaagaa aaagcccttc
     6241 aagagttaga taccgaagat cagcgtcatt tatatctgat gctgaaagag gattacgatt
     6301 caagcctgtt aattcccact attaaaaaag cgtttagcca gaatccaacg atgacaagac
     6361 aaaagttact gcctcttgtt ttgcagtggt tgatggaagg cgaaacggta gtgtcagaac
     6421 tagaaaagcc ctccaagagt aaaaaggttt cggctataaa ggtagtcaag cccagcgact
     6481 gggatagctt gcctgatacg gatttacgtt atatctattc acaacgccaa cctgaaaaaa
     6541 ccatgcatga acggttaaaa gggaaagggg taatagtgga tatggcgagc ttatttaaac
     6601 aagcaggtta gccatgagaa actttcctgt tccgtactcg aatgagctga tttatagcac
     6661 tattgcacgg gcaggcgttt atcaagggat tgttagtcct aagcagctgt tggatgaggt
     6721 gtatggcaac cgcaaggtgg tcgctacctt aggtctgccc tcgcatttag gtgtgatagc
     6781 aagacatcta catcaaacag gacgttacgc tgttcagcag cttatttatg agcatacctt
     6841 attcccttta tatgctccgt ttgtaggcaa ggagcgccga gacgaagcta ttcggttaat
     6901 ggagtaccaa gcgcaaggtg cggtgcattt aatgctagga gtcgctgctt ctagagttaa
     6961 gagcgataac cgctttagat actgccctga ttgcgttgct cttcagctaa ataggtatgg
     7021 ggaagccttt tggcaacgag attggtattt gcccgctttg ccatattgtc caaaacacgg
     7081 tgctttagtc ttctttgata gagctgtaga tgatcaccga catcaatttt gggctttggg
     7141 tcatactgag ctgctttcag actaccccaa agactcccta tctcaattaa cagcactagc
     7201 tgcttatata gcccctctgt tagatgctcc acgagcgcaa gagctttccc caagccttga
     7261 gcagtggacg ctgttttatc agcgcttagc gcaggatcta gggctaacca aaagcaagca
     7321 cattcgtcat gacttggtgg cggagagagt gaggcagact tttagtgatg aggcactaga
     7381 gaaactggat ttaaagttgg cagagaacaa ggacacgtgt tggctgaaaa gtatattccg
     7441 taagcataga aaagccttta gttatttaca gcatagtatt gtgtggcaag ccttattgcc
     7501 aaaactaacg gttatagaag cgctacagca ggcaagtgct cttactgagc actctataac
     7561 gacaagacct gttagccagt ctgtgcaacc taactctgaa gatttatctg ttaagcataa
     7621 agactggcag caactagtgc ataaatacca aggaattaag gcggcaagac agtctttaga
     7681 gggtggggtg ctatacgctt ggctttaccg acatgacagg gattggctag ttcactggaa
     7741 tcaacagcat caacaagagc gtctggcacc cgcccctaga gttgattgga accaaagaga
     7801 tcgaattgct gtacgacaac tattaagaat cataaagcgt ctagatagta gccttgatca
     7861 cccaagagcg acatcgagct ggctgttaaa gcaaactcct aacggaacct ctcttgcaaa
     7921 aaatctacag aaactgcctt tggtagcgct ttgcttaaag cgttactcag agagtgtgga
     7981 agattatcaa attagacgga ttagccaagc ttttattaag cttaaacagg aagatgttga
     8041 gcttaggcgc tggcgattat taagaagtgc aacgttatct aaagagcgga taactgagga
     8101 agcacaaaga ttcttggaaa tggtttatgg ggaagagtga gtggttaggc tagctacatt
     8161 taatgacaat gtgcaggttg tacatattgg tcatttattc cgtaactcgg gtcataagga
     8221 gtggcgtatt tttgtttggt ttaatccaat gcaagaacgg aaatggactc gatttactca
     8281 tttgccttta ttaagtcgag ctaaggtggt taacagtaca acaaagcaaa taaataaggc
     8341 ggatcgtgtg attgagtttg aagcatcgga tcttcaacga gccaaaataa tcgattttcc
     8401 taatctctcg tcctttgctt ccgtacgcaa caaggatgga gcgcagagtt catttattta
     8461 cgaagctgaa acaccatata gcaagactcg ttatcacatc ccacagttag agctagctcg
     8521 gtcattattt ttaggggatc ctctagagtc gacctgcagg catgcaagct tggctgtttt
     8581 ggcggatgag agaagatttt cagcctgata cagattaaat cagaacgcag aagcggtctg
     8641 ataaaacaga atttgcctgg cggcagtagc gcggtggtcc cacctgaccc catgccgaac
     8701 tcagaagtga aacgccgtag cgccgatggt agtgtggggt ctccccatgc gagagtaggg
     8761 aactgccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat
     8821 ctgttgtttg tcggtgaacg ctctcctgag taggacaaat ccgccgggag cggatttgaa
     8881 cgttgcgaag caacggcccg gagggtggcg ggcaggacgc ccgccataaa ctgccaggca
     8941 tcaaattaag cagaaggcca tcctgacgga tggccttttt gcgtttctac aaactctttt
     9001 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     9061 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     9121 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     9181 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     9241 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     9301 aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc
     9361 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     9421 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     9481 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     9541 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     9601 taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac
     9661 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     9721 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     9781 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     9841 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     9901 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     9961 aagtttactc atatatactt tagattgatt tacgcgccct gtagcggcgc attaagcgcg
    10021 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
    10081 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg ccggccagcc tcgcagagca
    10141 ggattcccgt tgagcaccgc caggtgcgaa taagggacag tgaagaagga acacccgctc
    10201 gcgggtgggc ctacttcacc tatcctgccc ggcggcatca ccggcgccac aggtgcggtt
    10261 gctggcgcct atatcgccga catcaccgat ggggaagatc gggctcgcca cttcgggctc
    10321 atgagcgctt gtttcggcgt gggtatggtg gcaggccccg tggccggggg actgttgggc
    10381 gccatctcct tgcatgcacc attccttgcg gcggcggtgc tcaacggcct caacctacta
    10441 ctgggctgct tcctaatgca ggagtcgcat aagggagagc gtcgatcccc gacagtaaga
    10501 cgggtaagcc tgttgatgat accgctgcct tactgggtgc attagccagt ctgaatgacc
    10561 tgtcacggga taatccgaag tggtcagact ggaaaatcag agggcaggaa ctgctgaaca
    10621 gcaaaaagtc agatagcacc acatagcaga cccgccataa aacgccctga gaagcccgtg
    10681 acgggctttt cttgtattat gggtagtttc cttgcatgaa tccataaaag gcgcctgtag
    10741 tgccatttac ccccattcac tgccagagcc gtgagcgcag cgaactgaat gtcacgaaaa
    10801 agacagcgac tcaggtgcct gatggtcgga gacaaaagga atattcagcg atttgcccga
    10861 gcttgcgagg gtgctactta agcctttagg gttttaaggt ctgttttgta gaggagcaaa
    10921 cagcgtttgc gacatccttt tgtaatactg cggaactgac taaagtagtg agttatacac
    10981 agggctggga tctattcttt ttatcttttt ttattctttc tttattctat aaattataac
    11041 cacttgaata taaacaaaaa aaacacacaa aggtctagcg gaatttacag agggtctagc
    11101 agaatttaca agttttccag caaaggtcta gcagaattta cagataccca caactcaaag
    11161 gaaaaggact agtaattatc attgactagc ccatctcaat tggtatagtg attaaaatca
    11221 cctagaccaa ttgagatgta tgtctgaatt agttgttttc aaagcaaatg aactagcgat
    11281 tagtcgctat gacttaacgg agcatgaaac caagctaatt ttatgctgtg tggcactact
    11341 caaccccacg attgaaaacc ctacaaggaa agaacggacg gtatcgttca cttataacca
    11401 atacgttcag atgatgaaca tcagtaggga aaatgcttat ggtgtattag ctaaagcaac
    11461 cagagagctg atgacgagaa ctgtggaaat caggaatcct ttggttaaag gctttgagat
    11521 tttccagtgg acaaactatg ccaagttctc aagcgaaaaa ttagaattag tttttagtga
    11581 agagatattg ccttatcttt tccagttaaa aaaattcata aaatataatc tggaacatgt
    11641 taagtctttt gaaaacaaat actctatgag gatttatgag tggttattaa aagaactaac
    11701 acaaaagaaa actcacaagg caaatataga gattagcctt gatgaattta agttcatgtt
    11761 aatgcttgaa aataactacc atgagtttaa aaggcttaac caatgggttt tgaaaccaat
    11821 aagtaaagat ttaaacactt acagcaatat gaaattggtg gttgataagc gaggccgccc
    11881 gactgatacg ttgattttcc aagttgaact agatagacaa atggatctcg taaccgaact
    11941 tgagaacaac cagataaaaa tgaatggtga caaaatacca acaaccatta catcagattc
    12001 ctacctacat aacggactaa gaaaaacact acacgatgct tt
//
