LOCUS       sequence-27464-e         860 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Scott KL, Kabbarah O, Liang MC, Ivanova E, Anagnostou V, Wu J, 
            Dhakal S, Wu M, Chen S, Feinberg T, Huang J, Saci A, Widlund HR, 
            Fisher DE, Xiao Y, Rimm DL, Protopopov A, Wong KK, Chin L
  TITLE     GOLPH3 modulates mTOR signalling and rapamycin sensitivity in 
            cancer.
  JOURNAL   Nature. 2009 Jun 25. 459(7250):1085-90.
  PUBMED    19553991
REFERENCE   2  (bases 1 to 860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2009 Jun 25. 459(7250):1085-90."
COMMENT     Sequence Label: pEF-DEST51-GOLPH3
FEATURES             Location/Qualifiers
     source          1..860
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(22..63)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    89..113
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 atggtgatga tgaccggtac gcgtagaatc gagaccgagg agagggttag ggataggctt
       61 accttcgaac cgcgggccct ctagatcaac cactttgtac aagaaagttg ggtacttggt
      121 gaacgccgcc accaccgccc acagaacctc attggtgttg gccttcagac attccacttc
      181 agggtctaag tcgagaagct gccgcactct cttggtagcc aaatcatact gctcgtccag
      241 aagaggagca aaagcattct ccaggacgtc cgaggcatga gccaggtaaa tgagggccag
      301 caagcgcctg tccatgcggt gagggtcatt cacccatttg tcaagaacgg cttcctgtac
      361 tttcttgatg aggcgctgct taatgttgtt attggtgagg ggatgtgttg tcatgtcaaa
      421 aagtaggaag ttctgtttct ctgttgtcaa tacacccttt tccaccaggt ttttagctaa
      481 tcgttcccgt acatttctta actgataatg caattttaat ggattccatg tctcaccact
      541 aagtaattca atccagttct ggaccgtttc tggaggctga gtttccttaa catgcttcag
      601 agcttcatca agaagaacat cccctgttgg agcatctgac ttacagatta cctttcttgt
      661 taatagactt ttacgtctca ttccacaagc ctctagttgt aaccttcctc tcaatgctaa
      721 ttcaattaac atacagccac gtaatccaga tgatatacag tcattccaaa atgatgtgta
      781 accctcgcgg tcttgaggcc agcaggagca cttcctcatc aggtcagccc gttcttggat
      841 cgccttgtct cgtcgtctgc
//
