LOCUS       sequence-274640-       17041 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  TcTT2  in Ti plasmid binary vector pGZ12.0501 (KF871320.1).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17041)
  AUTHORS   Florez SL, Erwin RL, Maximova SN, Guiltinan MJ, Curtis WR
  TITLE     Enhanced somatic embryogenesis in Theobroma cacao using the 
            homologous BABY BOOM transcription factor.
  JOURNAL   BMC Plant Biol. 2015 May 16;15:121. doi: 10.1186/s12870-015-0479-4.
  PUBMED    25976599
REFERENCE   2  (bases 1 to 17041)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Plant 
            Biol."; date: "2015-05-16"; volume: "15:121. doi"; pages: " 
            10.1186/s12870-015-0479-4"
COMMENT     Sequence Label: pGZ12.0501
FEATURES             Location/Qualifiers
     source          1..17041
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    393..417
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        541..721
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        573..757
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             778..1572
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     terminator      1962..2214
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        3192..3237
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     protein_bind    3529..3540
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
     promoter        5061..5106
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     CDS             5206..6000
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    6204..6378
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        7095..7140
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     regulatory      7240..7249
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(7246..7248,7249..7251,7252..7965)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(7246..7248,7249..7251,7252..7962)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7291..7312)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7552..7571)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7899..7920
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    8003..8177
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     primer_bind     complement(8208..8225)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8208..8224)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8217..8239)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(8364..8383)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8574..8595
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    8842..8866
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(10462..11610)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(11909..12703)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  12895..13662
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      14285..14996
                     /label=oriV
                     /note="incP origin of replication"
     oriT            15526..15635
                     /note="incP origin of transfer"
     primer_bind     15858..15877
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     16111..16128
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(16316..16966)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
ORIGIN
        1 tcggaccagc ggaggctggt ccggaggcca gacgtgaaac ccaacatacc cctgatcgta
       61 attctgagca ctgtcgcgct cgacgctgtc ggcatcggcc tgattatgcc ggtgctgccg
      121 ggcctcctgc gcgatctggt tcactcgaac gacgtcaccg cccactatgg cattctgctg
      181 gcgctgtatg cgttggtgca atttgcctgc gcacctgtgc tgggcgcgct gtcggatcgt
      241 ttcgggcggc ggccaatctt gctcgtctcg ctggccggcg ccagatctgg ggaaccctgt
      301 ggttggcatg cacatacaaa tggacgaacg gataaacctt ttcacgccct tttaaatatc
      361 cgattattct aataaacgct cttttctctt aggtttaccc gccaatatat cctgtcaaac
      421 actgatagtt taaactgaag gcgggaaacg acaatctgat catgagcgga gaattaaggg
      481 agtcacgtta tgacccccgc cgatgacgcg ggacaagccg ttttacgttt ggaactgaca
      541 gaaccgcaac gttgaaggag ccactcagcc gcgggtttct ggagtttaat gagctaagca
      601 catacgtcag aaaccattat tgcgcgttca aaagtcgcct aaggtcacta tcagctagca
      661 aatatttctt tgtcaaaaat gctccactga cgttccataa attcccctcg gtatccaatt
      721 agagtctcat attcactctc aatccaaata atctgcaccg gatctggatc gtttcgcatg
      781 attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc
      841 tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg
      901 caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag
      961 gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc
     1021 gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat
     1081 ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg
     1141 cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc
     1201 gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag
     1261 catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgcgcat gcccgacggc
     1321 gatgatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc
     1381 cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata
     1441 gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc
     1501 gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac
     1561 gagttcttct gagcgggact ctggggttcg aaatgaccga ccaagcgacg cccaacctgc
     1621 catcacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt
     1681 tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc
     1741 acgggatctc tgcggaacag gcggtcgaag gtgccgatat cattacgaca gcaacggccg
     1801 acaagcacaa cgccacgatc ctgagcgaca atatgatcgg gcccggcgtc cacatcaacg
     1861 gcgtcggcgg cgactgccca ggcaagaccg agatgcaccg cgatatcttg ctgcgttcgg
     1921 atattttcgt ggagttcccg ccacagaccc ggatgatccc cgatcgttca aacatttggc
     1981 aataaagttt cttaagattg aatcctgttg ccggtcttgc gatgattatc atataatttc
     2041 tgttgaatta cgttaagcat gtaataatta acatgtaatg catgacgtta tttatgagat
     2101 gggtttttat gattagagtc ccgcaattat acatttaata cgcgatagaa aacaaaatat
     2161 agcgcgcaaa ctaggataaa ttatcgcgcg cggtgtcatc tatgttacta gatcgggcct
     2221 cctgtcaatg ctggcggcgg ctctggtggt ggttctggtg gcggctctga gggtggtggc
     2281 tctgagggtg gcggttctga gggtggcggc tctgagggag gcggttccgg tggtggctct
     2341 ggttccggtg attttgatta tgaaaagatg gcaaacgcta ataagggggc tatgaccgaa
     2401 aatgccgatg aaaacgcgct acagtctgac gctaaaggca aacttgattc tgtcgctact
     2461 gattacggtg ctgctatcga taggcctagc ttgccaacat ggtggagcac gacactctcg
     2521 tctactccaa gaatatcaaa gatacagtct cagaagacca aagggctatt gagacttttc
     2581 aacaaagggt aatatcggga aacctcctcg gattccattg cccagctatc tgtcacttca
     2641 tcaaaaggac agtagaaaag gaaggtggca cctacaaatg ccatcattgc gataaaggaa
     2701 aggctatcgt tcaagatgcc tctgccgaca gtggtcccaa agatggaccc ccacccacga
     2761 ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc aaagcaagtg gattgatgtg
     2821 aacatggtgg agcacgacac tctcgtctac tccaagaata tcaaagatac agtctcagaa
     2881 gaccaaaggg ctattgagac ttttcaacaa agggtaatat cgggaaacct cctcggattc
     2941 cattgcccag ctatctgtca cttcatcaaa aggacagtag aaaaggaagg tggcacctac
     3001 aaatgccatc attgcgataa aggaaaggct atcgttcaag atgcctctgc cgacagtggt
     3061 cccaaagatg gacccccacc cacgaggagc atcgtggaaa aagaagacgt tccaaccacg
     3121 tcttcaaagc aagtggattg atgtgatatc tccactgacg taagggatga cgcacaatcc
     3181 cactatcctt cgcaagaccc ttcctctata taaggaagtt catttcattc ggagaggtac
     3241 gtatttttac aacaattacc aacaacaaca acaaacaaca acaacattac attttacatt
     3301 ctacaaacta gtatggggag ggctccttgc tgctcaaaag tggggctgca caggggacct
     3361 tggacaccta gggaagacac actgctcgtg aagtatatcc aggcccacgg agatggtcat
     3421 tggagaagct tgcctaagaa agctggcctc cttagatgcg ggaagtcatg tagactcagg
     3481 tggatgaact accttagacc tgacataaaa aggggcaata ttactccaga tgaggatgac
     3541 ctcattatca gacttcacag cttgctgggt aacaggtggt cattgatcgc tggcagactg
     3601 ccagggagga ctgacaatga gattaagaac tattggaata cacacctttc aaaaagactc
     3661 ctttctcagg gaactgatcc taacacacat aagaaattga gcgaaccagc agttcagcaa
     3721 gtgaagaaaa gaaagagctc aaggggaaac tctaataaga agcaaaacaa tagcaagggc
     3781 aaaggggcca aggttgagcc tgaaaagcca aaagtgcatt tgcctaaacc agttagagtg
     3841 acttctttca gcctgcctag gaacgactca ttcgatcagt gcaatacttt ttctacagtt
     3901 tctagctcac aaggaggaga gggcgggctc ggaactgaag ttgtgcaagg tccatggtct
     3961 gataacgtta atgatgacga gaacggaaca ggttttcttg ctgcatacga tgaccacgga
     4021 ttcgttaatg gttcagattt tgagtgtcag agccatgtgc ctgcatcaga tgacgataat
     4081 tctttggaaa agctgtacga ggaatatctt caattgctca agactaacga cgaccaggtt
     4141 cagttggact catttgccga atcattgctc atctaagtta actttctcca taataatgtg
     4201 tgagtagttc ccagataagg gaattagggt tcctataggg tttcgctcat gtgttgagca
     4261 tataagaaac ccttagtatg tatttgtatt tgtaaaatac ttctatcaat aaaatttcta
     4321 attcctaaaa ccaaaatcca gtcctaggaa tcgataagct tgccaacatg gtggagcacg
     4381 acactctcgt ctactccaag aatatcaaag atacagtctc agaagaccaa agggctattg
     4441 agacttttca acaaagggta atatcgggaa acctcctcgg attccattgc ccagctatct
     4501 gtcacttcat caaaaggaca gtagaaaagg aaggtggcac ctacaaatgc catcattgcg
     4561 ataaaggaaa ggctatcgtt caagatgcct ctgccgacag tggtcccaaa gatggacccc
     4621 cacccacgag gagcatcgtg gaaaaagaag acgttccaac cacgtcttca aagcaagtgg
     4681 attgatgtga acatggtgga gcacgacact ctcgtctact ccaagaatat caaagataca
     4741 gtctcagaag accaaagggc tattgagact tttcaacaaa gggtaatatc gggaaacctc
     4801 ctcggattcc attgcccagc tatctgtcac ttcatcaaaa ggacagtaga aaaggaaggt
     4861 ggcacctaca aatgccatca ttgcgataaa ggaaaggcta tcgttcaaga tgcctctgcc
     4921 gacagtggtc ccaaagatgg acccccaccc acgaggagca tcgtggaaaa agaagacgtt
     4981 ccaaccacgt cttcaaagca agtggattga tgtgatatct ccactgacgt aagggatgac
     5041 gcacaatccc actatccttc gcaagaccct tcctctatat aaggaagttc atttcattcg
     5101 gagaggtacg tatttttaca acaattacca acaacaacaa caaacaacaa caacattaca
     5161 ttttacattc tacaactaca tctagaattc cccggatcgt ttcgcatgat tgaacaagat
     5221 ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca
     5281 caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg
     5341 gttctttttg tcaagaccga cctgtccggt gccctgaatg aactccagga cgaggcagcg
     5401 cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact
     5461 gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct
     5521 caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg
     5581 cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt
     5641 actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc
     5701 gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc
     5761 gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga
     5821 ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc
     5881 cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt
     5941 atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga
     6001 gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt
     6061 tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg
     6121 gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac cccggaattc
     6181 tagaattggc atgcaagctc gagtttctcc ataataatgt gtgagtagtt cccagataag
     6241 ggaattaggg ttcctatagg gtttcgctca tgtgttgagc atataagaaa cccttagtat
     6301 gtatttgtat ttgtaaaata cttctatcaa taaaatttct aattcctaaa accaaaatcc
     6361 agtactaaaa tccagatccc ccgaattcca agcttgccaa catggtggag cacgacactc
     6421 tcgtctactc caagaatatc aaagatacag tctcagaaga ccaaagggct attgagactt
     6481 ttcaacaaag ggtaatatcg ggaaacctcc tcggattcca ttgcccagct atctgtcact
     6541 tcatcaaaag gacagtagaa aaggaaggtg gcacctacaa atgccatcat tgcgataaag
     6601 gaaaggctat cgttcaagat gcctctgccg acagtggtcc caaagatgga cccccaccca
     6661 cgaggagcat cgtggaaaaa gaagacgttc caaccacgtc ttcaaagcaa gtggattgat
     6721 gtgaacatgg tggagcacga cactctcgtc tactccaaga atatcaaaga tacagtctca
     6781 gaagaccaaa gggctattga gacttttcaa caaagggtaa tatcgggaaa cctcctcgga
     6841 ttccattgcc cagctatctg tcacttcatc aaaaggacag tagaaaagga aggtggcacc
     6901 tacaaatgcc atcattgcga taaaggaaag gctatcgttc aagatgcctc tgccgacagt
     6961 ggtcccaaag atggaccccc acccacgagg agcatcgtgg aaaaagaaga cgttccaacc
     7021 acgtcttcaa agcaagtgga ttgatgtgat atctccactg acgtaaggga tgacgcacaa
     7081 tcccactatc cttcgcaaga cccttcctct atataaggaa gttcatttca ttcggagagg
     7141 tacgtatttt tacaacaatt accaacaaca acaacaaaca acaacaacat tacattttac
     7201 attctacaac tacatctaga ggatccccgg gtaccggtcg ccaccatggt gagcaagggc
     7261 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc
     7321 cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg
     7381 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg
     7441 acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc
     7501 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc
     7561 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag
     7621 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac
     7681 tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac
     7741 ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag
     7801 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag
     7861 tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
     7921 accgccgccg ggatcactct cggcatggac gagctgtaca agtaaagcgg ccgcgactct
     7981 agaattggca tgcaagctcg agtttctcca taataatgtg tgagtagttc ccagataagg
     8041 gaattagggt tcctataggg tttcgctcat gtgttgagca tataagaaac ccttagtatg
     8101 tatttgtatt tgtaaaatac ttctatcaat aaaatttcta attcctaaaa ccaaaatcca
     8161 gtactaaaat ccagatcccc cgaattccgg gtaccgagct cgaattcact ggccgtcgtt
     8221 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat
     8281 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
     8341 ttgcgcagcc tgaatggcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
     8401 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
     8461 tttacggcac ctcgacccca aaaaacttga tttgggtgat ggttcacgta gtgggccatc
     8521 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
     8581 cttgttccaa actggaacaa cactcaaccc tatctcgggc tattcttttg atttataagg
     8641 gattttgccg atttcggaac caccatcaaa caggattttc gcctgctggg gcaaaccagc
     8701 gtggaccgct tgctgcaact ctctcagggc caggcggtga agggcaatca gctgttgccc
     8761 gtctcactgg tgaaaagaaa aaccacccca gtacattaaa aacgtccgca atgtgttatt
     8821 aagttgtcta agcgtcaatt tgtttacacc acaatatatc ctgccaccag ccagccaaca
     8881 gctccccgac cggcagctcg gcacaaaatc accactcgat acaggcagcc catcagtccg
     8941 ggacggcgtc agcgggagag ccgttgtaag gcggcagact ttgctcatgt taccgatgct
     9001 attcggaaga acggcaacta agctgccggg tttgaaacac ggatgatctc gcggagggta
     9061 gcatgttgat tgtaacgatg acagagcgtt gctgcctgtg atcaaatatc atctccctcg
     9121 cagagatccg aattatcagc cttcttattc atttctcgct taaccgtgac aggctgtcga
     9181 tcttgagaac tatgccgaca taataggaaa tcgctggata aagccgctga ggaagctgag
     9241 tggcgctatt tctttagaag tgaacgttga cgatatcaac tcccctatcc attgctcacc
     9301 gaatggtaca ggtcggggac ccgaagttcc gactgtcggc ctgatgcatc cccggctgat
     9361 cgaccccaga tctggggctg agaaagccca gtaaggaaac aactgtaggt tcgagtcgcg
     9421 agatcccccg gaaccaaagg aagtaggtta aacccgctcc gatcaggccg agccacgcca
     9481 ggccgagaac attggttcct gtaggcatcg ggattggcgg atcaaacact aaagctactg
     9541 gaacgagcag aagtcctccg gccgccagtt gccaggcggt aaaggtgagc agaggcacgg
     9601 gaggttgcca cttgcgggtc agcacggttc cgaacgccat ggaaaccgcc cccgccaggc
     9661 ccgctgcgac gccgacagga tctagcgctg cgtttggtgt caacaccaac agcgccacgc
     9721 ccgcagttcc gcaaatagcc cccaggaccg ccatcaatcg tatcgggcta cctagcagag
     9781 cggcagagat gaacacgacc atcagcggct gcacagcgcc taccgtcgcc gcgaccccgc
     9841 ccggcaggcg gtagaccgaa ataaacaaca agctccagaa tagcgaaata ttaagtgcgc
     9901 cgaggatgaa gatgcgcatc caccagattc ccgttggaat ctgtcggacg atcatcacga
     9961 gcaataaacc cgccggcaac gcccgcagca gcataccggc gacccctcgg cctcgctgtt
    10021 cgggctccac gaaaacgccg gacagatgcg ccttgtgagc gtccttgggg ccgtcctcct
    10081 gtttgaagac cgacagccca atgatctcgc cgtcgatgta ggcgccgaat gccacggcat
    10141 ctcgcaaccg ttcagcgaac gcctccatgg gctttttctc ctcgtgctcg taaacggacc
    10201 cgaacatctc tggagctttc ttcagggccg acaatcggat ctcgcggaaa tcctgcacgt
    10261 cggccgctcc aagccgtcga atctgagcct taatcacaat tgtcaatttt aatcctctgt
    10321 ttatcggcag ttcgtagagc gcgccgtgcg tcccgagcga tactgagcga agcaagtgcg
    10381 tcgagcagtg cccgcttgtt cctgaaatgc cagtaaagcg ctggctgctg aacccccagc
    10441 cggaactgac cccacaaggc cctagcgttt gcaatgcacc aggtcatcat tgacccaggc
    10501 gtgttccacc aggccgctgc ctcgcaactc ttcgcaggct tcgccgacct gctcgcgcca
    10561 cttcttcacg cgggtggaat ccgatccgca catgaggcgg aaggtttcca gcttgagcgg
    10621 gtacggctcc cggtgcgagc tgaaatagtc gaacatccgt cgggccgtcg gcgacagctt
    10681 gcggtacttc tcccatatga atttcgtgta gtggtcgcca gcaaacagca cgacgatttc
    10741 ctcgtcgatc aggacctggc aacgggacgt tttcttgcca cggtccagga cgcggaagcg
    10801 gtgcagcagc gacaccgatt ccaggtgccc aacgcggtcg gacgtgaagc ccatcgccgt
    10861 cgcctgtagg cgcgacaggc attcctcggc cttcgtgtaa taccggccat tgatcgacca
    10921 gcccaggtcc tggcaaagct cgtagaacgt gaaggtgatc ggctcgccga taggggtgcg
    10981 cttcgcgtac tccaacacct gctgccacac cagttcgtca tcgtcggccc gcagctcgac
    11041 gccggtgtag gtgatcttca cgtccttgtt gacgtggaaa atgaccttgt tttgcagcgc
    11101 ctcgcgcggg attttcttgt tgcgcgtggt gaacagggca gagcgggccg tgtcgtttgg
    11161 catcgctcgc atcgtgtccg gccacggcgc aatatcgaac aaggaaagct gcatttcctt
    11221 gatctgctgc ttcgtgtgtt tcagcaacgc ggcctgcttg gcctcgctga cctgttttgc
    11281 caggtcctcg ccggcggttt ttcgcttctt ggtcgtcata gttcctcgcg tgtcgatggt
    11341 catcgacttc gccaaacctg ccgcctcctg ttcgagacga cgcgaacgct ccacggcggc
    11401 cgatggcgcg ggcagggcag ggggagccag ttgcacgctg tcgcgctcga tcttggccgt
    11461 agcttgctgg accatcgagc cgacggactg gaaggtttcg cggggcgcac gcatgacggt
    11521 gcggcttgcg atggtttcgg catcctcggc ggaaaacccc gcgtcgatca gttcttgcct
    11581 gtatgccttc cggtcaaacg tccgattcat tcaccctcct tgcgggattg ccccgactca
    11641 cgccggggca atgtgccctt attcctgatt tgacccgcct ggtgccttgg tgtccagata
    11701 atccacctta tcggcaatga agtcggtccc gtagaccgtc tggccgtcct tctcgtactt
    11761 ggtattccga atcttgccct gcacgaatac cagcgacccc ttgcccaaat acttgccgtg
    11821 ggcctcggcc tgagagccaa aacacttgat gcggaagaag tcggtgcgct cctgcttgtc
    11881 gccggcatcg ttgcgccaca tctaggtact aaaacaattc atccagtaaa atataatatt
    11941 ttattttctc ccaatcaggc ttgatcccca gtaagtcaaa aaatagctcg acatactgtt
    12001 cttccccgat atcctccctg atcgaccgga cgcagaaggc aatgtcatac cacttgtccg
    12061 ccctgccgct tctcccaaga tcaataaagc cacttacttt gccatctttc acaaagatgt
    12121 tgctgtctcc caggtcgccg tgggaaaaga caagttcctc ttcgggcttt tccgtcttta
    12181 aaaaatcata cagctcgcgc ggatctttaa atggagtgtc ttcttcccag ttttcgcaat
    12241 ccacatcggc cagatcgtta ttcagtaagt aatccaattc ggctaagcgg ctgtctaagc
    12301 tattcgtata gggacaatcc gatatgtcga tggagtgaaa gagcctgatg cactccgcat
    12361 acagctcgat aatcttttca gggctttgtt catcttcata ctcttccgag caaaggacgc
    12421 catcggcctc actcatgagc agattgctcc agccatcatg ccgttcaaag tgcaggacct
    12481 ttggaacagg cagctttcct tccagccata gcatcatgtc cttttcccgt tccacatcat
    12541 aggtggtccc tttataccgg ctgtccgtca tttttaaata taggttttca ttttctccca
    12601 ccagcttata taccttagca ggagacattc cttccgtatc ttttacgcag cggtattttt
    12661 cgatcagttt tttcaattcc ggtgatattc tcattttagc catttattat ttccttcctc
    12721 ttttctacag tatttaaaga taccccaaga agctaattat aacaagacga actccaattc
    12781 actgttcctt gcattctaaa accttaaata ccagaaaaca gctttttcaa agttgttttc
    12841 aaagttggcg tataacatag tatcgacgga gccgattttg aaaccacaat tatgggtgat
    12901 gctgccaact tactgattta gtgtatgatg gtgtttttga ggtgctccag tggcttctgt
    12961 ttctatcagc tgtccctcct gttcagctac tgacggggtg gtgcgtaacg gcaaaagcac
    13021 cgccggacat cagcgctatc tctgctctca ctgccgtaaa acatggcaac tgcagttcac
    13081 ttacaccgct tctcaacccg gtacgcacca gaaaatcatt gatatggcca tgaatggcgt
    13141 tggatgccgg gcaacagccc gcattatggg cgttggcctc aacacgattt tacgtcactt
    13201 aaaaaactca ggccgcagtc ggtaacctcg cgcatacagc cgggcagtga cgtcatcgtc
    13261 tgcgcggaaa tggacgaaca gtggggctat gtcggggcta aatcgcgcca gcgctggctg
    13321 ttttacgcgt atgacagtct ccggaagacg gttgttgcgc acgtattcgg tgaacgcact
    13381 atggcgacgc tggggcgtct tatgagcctg ctgtcaccct ttgacgtggt gatatggatg
    13441 acggatggct ggccgctgta tgaatcccgc ctgaagggaa agctgcacgt aatcagcaag
    13501 cgatatacgc agcgaattga gcggcataac ctgaatctga ggcagcacat ggcacggctg
    13561 ggacggaagt cgctgtcgtt ctcaaaatcg gtggagctgc atgacaaagt catcgggcat
    13621 tatctgaaca taaaacacta tcaataagtt ggagtcatta cccaattatg atagaattta
    13681 caagctataa ggttattgtc ctgggtttca agcattagtc catgcaagtt tttatgcttt
    13741 gcccattcta tagatatatt gataagcgcg ctgcctatgc cttgccccct gaaatcctta
    13801 catacggcga tatcttctat ataaaagata tattatctta tcagtattgt caatatattc
    13861 aaggcaatct gcctcctcat cctcttcatc ctcttcgtct tggtagcttt ttaaatatgg
    13921 cgcttcatag agtaattctg taaaggtcca attctcgttt tcatacctcg gtataatctt
    13981 acctatcacc tcaaatggtt cgctgggttt atcgcacccc cgaacacgag cacggcaccc
    14041 gcgaccacta tgccaagaat gcccaaggta aaaattgccg gccccgccat gaagtccgtg
    14101 aatgccccga cggccgaagt gaagggcagg ccgccaccca ggccgccgcc ctcactgccc
    14161 ggcacctggt cgctgaatgt cgatgccagc acctgcggca cgtcaatgct tccgggcgtc
    14221 gcgctcgggc tgatcgccca tcccgttact gccccgatcc cggcaatggc aaggactgcc
    14281 agcgctgcca tttttggggt gaggccgttc gcggccgagg ggcgcagccc ctggggggat
    14341 gggaggcccg cgttagcggg ccgggagggt tcgagaaggg ggggcacccc ccttcggcgt
    14401 gcgcggtcac gcgcacaggg cgcagccctg gttaaaaaca aggtttataa atattggttt
    14461 aaaagcaggt taaaagacag gttagcggtg gccgaaaaac gggcggaaac ccttgcaaat
    14521 gctggatttt ctgcctgtgg acagcccctc aaatgtcaat aggtgcgccc ctcatctgtc
    14581 agcactctgc ccctcaagtg tcaaggatcg cgcccctcat ctgtcagtag tcgcgcccct
    14641 caagtgtcaa taccgcaggg cacttatccc caggcttgtc cacatcatct gtgggaaact
    14701 cgcgtaaaat caggcgtttt cgccgatttg cgaggctggc cagctccacg tcgccggccg
    14761 aaatcgagcc tgcccctcat ctgtcaacgc cgcgccgggt gagtcggccc ctcaagtgtc
    14821 aacgtccgcc cctcatctgt cagtgagggc caagttttcc gcgaggtatc cacaacgccg
    14881 gcggccgcgg tgtctcgcac acggcttcga cggcgtttct ggcgcgtttg cagggccata
    14941 gacggccgcc agcccagcgg cgagggcaac cagcccggtg agcgtcggaa aggcgctcgg
    15001 tcttgccttg ctcgtcggtg atgtacttca ccagctccgc gaagtcgctc ttcttgatgg
    15061 agcgcatggg gacgtgcttg gcaatcacgc gcaccccccg gccgttttag cggctaaaaa
    15121 agtcatggct ctgccctcgg gcggaccacg cccatcatga ccttgccaag ctcgtcctgc
    15181 ttctcttcga tcttcgccag cagcgcgagg attgtggcat caccgaaccg cgccgtgcgc
    15241 gggtcgtcgg tggccagagt ttcagcaggc cgcccaggcg gcccaggtcg ccattgatgc
    15301 gggccagctc gcggacgtgc tcatagtcca cgacgcccgt gattttgtag ccctggccga
    15361 cggccagcag gtaggccgac aggctcatgc cggccgccgc cgccttttcc tcaatcgctc
    15421 ttcgttcgtc tggaaggcag tacaccttga taggtgggct gcccttcctg gttggcttgg
    15481 tttcatcagc catccgcttg ccctcatctg ttacgccggc ggtagccggc cagcctcgca
    15541 gagcaggatt cccgttgagc accgccaggt gcgaataagg gacagtgaag aaggaacacc
    15601 cgctcgcggg tgggcctact tcacctatcc tgcccggctg acgccgttgg atacaccaag
    15661 gaaagtctac acgaaccctt tggcaaaatc ctgtatatcg tgcgaaaaag gatggatata
    15721 ccgaaaaaat cgctataatg accccgaagc agggttatgc agcggaaaag cgccacgctt
    15781 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
    15841 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
    15901 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
    15961 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
    16021 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
    16081 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
    16141 cgccagaagg ccgccagaga ggccgagcgc ggccgtgagg cttggacgct aggacagggc
    16201 atgaaaaagc ccgtagcggg ctgctacggg cgtctgacgc ggtggaaagg gggaggggat
    16261 gttgtctaca tggctctgct gtagtgagtg ggttgcgctc cggcagcggt cctgatcaat
    16321 cgtcaccctt tctcggtcct tcaacgttcc tgacaacgag cctccttttc gccaatccat
    16381 cgacaatcac cgcgagtccc tgctcgaacg ctgcgtccgg accggcttcg tcgaaggcgt
    16441 ctatcgcggc ccgcaacagc ggcgagagcg gagcctgttc aacggtgccg ccgcgctcgc
    16501 cggcatcgct gtcgccggcc tgctcctcaa gcacggcccc aacagtgaag tagctgattg
    16561 tcatcagcgc attgacggcg tccccggccg aaaaacccgc ctcgcagagg aagcgaagct
    16621 gcgcgtcggc cgtttccatc tgcggtgcgc ccggtcgcgt gccggcatgg atgcgcgcgc
    16681 catcgcggta ggcgagcagc gcctgcctga agctgcgggc attcccgatc agaaatgagc
    16741 gccagtcgtc gtcggctctc ggcaccgaat gcgtatgatt ctccgccagc atggcttcgg
    16801 ccagtgcgtc gagcagcgcc cgcttgttcc tgaagtgcca gtaaagcgcc ggctgctgaa
    16861 cccccaaccg ttccgccagt ttgcgtgtcg tcagaccgtc tacgccgacc tcgttcaaca
    16921 ggtccagggc ggcacggatc actgtattcg gctgcaactt tgtcatgctt gacactttat
    16981 cactgataaa cataatatgt ccaccaactt atcagtgata aagaatccgc gcgttcaatc
    17041 g
//
