LOCUS       sequence-274641-        8877 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses hrp-1p::hrp-1HsLCWT::hrp-1 3'UTR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8877)
  AUTHORS   Ryan VH, Perdikari TM, Naik MT, Saueressig CF, Lins J, Dignon GL, 
            Mittal J, Hart AC, Fawzi NL
  TITLE     Tyrosine phosphorylation regulates hnRNPA2 granule protein 
            partitioning and reduces neurodegeneration.
  JOURNAL   EMBO J. 2021 Feb 1;40(3):e105001. doi: 10.15252/embj.2020105001. 
            Epub 2020 Dec 22.
  PUBMED    33349959
REFERENCE   2  (bases 1 to 8877)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J."; 
            date: "2021-02-1"; volume: "40(3):e105001. doi"; pages: " 
            10.15252/embj.2020105001. Epub 2020 Dec 22"
COMMENT     Sequence Label: pHA841 - hrp-1p::hrp-1HsLCWT
FEATURES             Location/Qualifiers
     source          1..8877
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..48)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     239..260
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     523..545
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     537..554
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     538..554
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     564..583
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        564..582
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6581..6597)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6582..6598)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(6627..6647)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(6627..6645)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6666..6682)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6666..6682)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6679..6701)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6690..6706
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6714..6720,6721..6738,6739..6744))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6759..6780
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6897..6914)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7068..7656)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7148..7167)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7827..8618,8619..8687))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8450..8469
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8688..8792)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      join(8819..8877,1..397)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
       61 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
      121 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
      181 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
      241 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
      301 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
      361 aacgcgaatt ttaacaaaat attaacgctt acaatttcca ttcgccattc aggctgcgca
      421 actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg
      481 gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta
      541 aaacgacggc cagtgagcgc gcgtaatacg actcactata gggcgaattg gagctccacc
      601 gcggtggcgg ccgcttcaat tcttcggagc aggcgtagga gctgagccca tcttcaatgc
      661 ctccgatttt ccaaaattaa acactttttc catgaattct tccattaatt tggcaattga
      721 gccaaatgaa aagccagaag agccagatgg agccggtgtt ggagttggag cagacagagt
      781 tgaacggagt tctggagcag ctggggttgg ggtcggagca gcaagagttg aacgtggttc
      841 agggtcggcg aggaactgaa aatctaggtt ttttaggaaa aattcgccta aaaacatgcc
      901 gaaaaaattt ccgaatttta gacaattctg gaaaaaattg tagtgaaatt gtctaaaaat
      961 tttggtaaaa gtttgcagaa aactgtcaga aaatgttaat ttcttacgtg tttccttgct
     1021 tgggacacta gaacagcacc gaataagaca aaaagtacaa aacacagctt catgttggaa
     1081 tttatgccag aaaaatagct gtaactcggc ttttatagaa tagaattggt tcctggtgat
     1141 gctggagttc tattaaacat gagaacagga gtatgaaccg tgggaagtgg tgttttggtt
     1201 ggagaggtgt tctgcacggc tcggcaattg tggtgcatca gtgtccacca aacatcaagc
     1261 ataacctgga tgataaggag acaagctttg gtgcaaaagt tttcaaattt taaagcaatt
     1321 tttaggactc ttcaaagcat gaaactgttt ctcaaggttt ccttctactt ctaactcctt
     1381 ctagatgcat cacggcctca aaatgtaaaa cctcagaaat ggtaccagag gttcaaaaat
     1441 gcaaatatca gggacctggg ctagatcttc tcgtttcgag ggctgggaaa ctgcccttcc
     1501 gtgtgaaagg ggcaattgtc aggcctggag catgatattg atcggttttg tgagaagacc
     1561 aacaaggtag ctaataaatc gatcctatat tgtattaaca catgtatttg aatcagatct
     1621 ttcatttctc attgactccc tcaaatttcc agcaagaatg gatgctacaa ttgtaagttt
     1681 aaccacaaat catttttaat tatacctgaa tttcttttca gaccaaaacc agaaaagtcg
     1741 tattcatata gtgttgttca agagattgtc agttgtatgt gcctaacaaa aaaaaatcta
     1801 tggcattcta aatttacaat ttcagcatag tcgctcgcca tgtgtcacca ctctttcgcc
     1861 aaaccatcga tttgatggat ccaggcattg agaaaattca tttactcagt tctgccagtc
     1921 cgtagtgtag tttttaccgg atatagagaa ttgaatctag aaaacctggc tgcctggcaa
     1981 gaagtcactg gtagtaacag gctgatgagg gcagccttcg gtgagctatc cgacgtgatg
     2041 ggcatgccga aacttagact aagaaagttc agttttgata acacatatgg actggttggg
     2101 ttatctggga aagagaatat gcaatctgtg gatgactgct tcaaggctat gaacttacca
     2161 catgcgaagg tgcttgaagt gactggaatc gactttgtgg acaatatttc gatggtgaag
     2221 tactttcgcc caggttatct cgagaaaatc cgcatgtatt cttgttattg ctcagctagc
     2281 caggagatga gtgaagaaat tgcaaatctc gagcagtgga agcaagcgaa acattttctg
     2341 tgcattggtg gtagaatgaa cattcctatt caaagatttt tccactttga ccagttctcc
     2401 atcggtcttc agactctatc aatcgatgat gctgttcaga taagagatgt gagttggtag
     2461 ttaaagtagc gttttgtaat gtattctata ttccagattc tcctcagatc gcccagcttc
     2521 gagtcagcaa ctatcgatgt tcaactactt gaaaaagaag ccattgagaa cgtctttacg
     2581 ccgaatcacc tattaattta tagaggaaga gagttcagac cggtgggaac tgattccagt
     2641 tttgtgatca atataacaaa aaacaagctt gaaatccaca agcacaaggg agctaatcca
     2701 ccacgtcgta gagacgaacg gccaatccac gagcgtatac gtcaaaggca agatggatgg
     2761 tgaagtttca atggttgctc taatacgttt acatgtctat caaacctttt tctgttactt
     2821 aagtaaatcg ttgtttcata tttaaccagt cttacctacg gctggcatat cggacgtccg
     2881 gcgtcagaac cttgtgcgtc tggttttaga tgtttgaaaa ttttggaaaa ttattaaagt
     2941 ctattgataa cctgtcaaaa ccggacgacc ggacgccaaa tatgacagcc ctagtcttac
     3001 cagtataata tttttataaa cctaaaacag gtatgaatat ctcttttttc tgcgcagcgc
     3061 tgggatgctt ctgtttcaga aaaatcacat ggtataaaaa atttccaaat tacagtagtc
     3121 ctaccgtaat cctgcattat cccactactg tatgtacctt gacattattt ttcctcgccc
     3181 aactcccagc ccaatatatc tcttcaacag ctaagaacta catttttcct aaactacagt
     3241 aaaccaacag tatccctaca gtaccacatt aattataaat taaaacatta tttttcataa
     3301 gaaatcgtga gaatttcgag aaaaataaag gattcaaaga ttatttgaac gtttttttcg
     3361 tgaaaaaatg tcaaagaaat caaaatgttc atcttccttg tgaaaaatcc gaaaagccat
     3421 tataaactag ttgattttca taaaaatttg tagaaatatt tttcttaaaa tttgtagaag
     3481 aattttaacg ttttttaaac aaaaattcca aaattggaat ttggtattga aaatactttt
     3541 taaaagttat ttttttaatt tgtaaaaatt taactaaatt caaaaaataa agtttccctc
     3601 ctttttttct taaaggcaca cacatttatc gactcgggtc tcgccgcgaa cagacgcgac
     3661 gcgacgcggt taggaatgcg gcgcgtcgcg tgtaaccctt ttctctgtgg ttttcgagag
     3721 cattctctct cggttttcgt cacaaaaagg tatgttttac aggttttccg cgcactgttt
     3781 tcgcgtaatt cttcatttta attcagataa tctaattgtt gtgtagtttt agctcgaatt
     3841 tatccgtaat ctttgattaa atacgaataa aactcgctaa aatagtctta tttctcagat
     3901 atttatcgat ttgttatttt ccacgaatag tctactcgtg catggcgtct atatttaact
     3961 taataatcta tttttagatt tatctttact ctaacttgta cctatttcag tgatcaacac
     4021 ctctgtcact ctttgtgagt aatagcgttc gataggtagt tacaccgtga aactagcctg
     4081 tctatttcct acttcataag ctaagaacta cttgttagca caatccgatc gactactaag
     4141 aactagatca tgacggacgt ggaaatcaag gctgaggtta cgtttgttca cttgctttta
     4201 gggtgtaggc cacctgattt gggtcccgat catttaatta tcgattttat tttcagaatg
     4261 gaagcggaga tgcctcgctc gagccagaga accttcgaaa gattttcgtt ggaggactca
     4321 cgtcaaacac caccgatgac ctgatgcgag agttctactc acaattcgga gaaatcactg
     4381 atatcatcgt catgagagat ccaactacca agagatcccg cggattcgga tttgtcacct
     4441 tctctggcaa aactgaagtc gatgctgcca tgaaacaacg cccgcacatc atcgacggaa
     4501 agaccgtgga cccgaagcgt gccgtgccac gtgatgataa gaaccgctcc gagtcgaatg
     4561 tctccaccaa acgcctgtac gtcagcggag ttcgtgagga tcacaccgag gacatgctca
     4621 ccgagtattt cacaaagtat ggaaccgtca ccaaatccga gattattctc gacaaagcca
     4681 cccaaaagcc aagaggcttc ggattcgtca ccttcgatga tcatgactct gtggatcaat
     4741 gcgttcttca aaaatcccat atggtcaacg gacacagatg tgacgtgcgc aaaggactct
     4801 ccaaggacga gatgagcaag gctcagatga accgcgatcg tgaaacccgt ggcggaagat
     4861 ctcgcgatgg acagcgtggc ggttacaatg gcggtggtgg tggcggcggc ggctggggag
     4921 gaccagctca acgcggcggt ccaggagcat atggtggacc aggaggtggc ggccaaggag
     4981 gatatggcgg aggtagttgg attctggctt gtgaattctc gattctaata ttattaactt
     5041 tcagattacg gaggaggacg cggtggttat ggtggtggag gaccaggata tggaaatcag
     5101 ggtggaggat atggaggagg atatgataat tatggaggag gaaattacgg atctggaaat
     5161 tacaatgatt tcggaaacta caaccagcag ccatctaatt atggaccaat gaagtctgga
     5221 aatttcggag gatctcgcaa tatgggtgga ccatatggag gaggaaatta tggtccagga
     5281 ggatctggag gatctggagg atatggagga cgttctcgtt actaaattaa ttccttaagc
     5341 ccctctaagt gtccaacgtg tctgagcttc ccgtgctctc tcctctgtat cgatcttctc
     5401 aaccattttt gctctgctat ctcatcaaaa tcttctgtac tttttcttgt tgcctttctc
     5461 cagaaagttt cgtttattat tcaatttttt accctgtctt ttttatataa tttatttcta
     5521 aattcgcaca gttgacatcc attcaactag agaatgaatg cagagtttga caccaaattg
     5581 gcttaatttt caagctgttt ttaaccaaaa taataaaaaa gtctcaactt tacaaaaatg
     5641 tttatctgga tctggacgac agcgagctga gaaattgata tattttcaga gtaaaaacta
     5701 aaaatgtctg gaatttttgg agtcattgtc tcagggagga cgccgatcga agtggtgccg
     5761 gtctccgata cggagttcac ctgcgaaatt gtaaacgctg acgcgataaa ccacgtggtg
     5821 gtcttcctta ccggagctca accgtttccg gacggaattg gtggctctgg tgatcatttt
     5881 cagaattttc cgcccaaaaa aacccaattt atttcagttt acatcagatg gcccacccaa
     5941 gacggcggaa attggcatta tttgggattt atttgcaacg agaagcccag cgcgatttat
     6001 aaagttgctc aggtgtgcat ttttgatgga aaaatgaaag aaaaccaccg aaatttcgat
     6061 gtttctctgt cgattttttt tatttaaaaa cttagaaata ctctcaattt cgcatttttc
     6121 accaaaaaaa agaatctcaa ctggtttttc acatagctta atttttgaag aaaaatctca
     6181 acaaaaccca gttttaagtt tcaaattctc aaaaattgat cttttttttc ccttaaaaat
     6241 ccagttttta gctccaaatt gtcataattt tccagaaaaa tttagttttc gagctaacaa
     6301 cattaaaatt tcacagaaaa tctgaatttc agccttagag atccaaattt ttcggctgaa
     6361 aatcacaatt tttccatttt tcagctccac aaatccgacg cttctcactc gttttcccaa
     6421 tttgacaatc aaatgcaact atattccagt ggatcagctc aaattggaat taatgctgaa
     6481 agtttatctg atatcactgg acggcaggca gctgatggga cacaggctag ccagcaggtg
     6541 aaggatcccc cgggctgcag gaattcgata tcaagcttat cgataccgtc gacctcgagg
     6601 gggggcccgg tacccagctt ttgttccctt tagtgagggt taattgcgcg cttggcgtaa
     6661 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     6721 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
     6781 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
     6841 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     6901 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     6961 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     7021 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     7081 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     7141 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     7201 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     7261 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     7321 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     7381 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     7441 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     7501 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     7561 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     7621 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     7681 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     7741 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     7801 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     7861 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     7921 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     7981 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     8041 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     8101 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     8161 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     8221 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     8281 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     8341 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     8401 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     8461 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     8521 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     8581 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     8641 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     8701 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     8761 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     8821 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgacc
//
