LOCUS       sequence-274644-        8877 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses hrp-1p::hrp-1HsLCD290V::hrp-1 3'UTR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8877)
  AUTHORS   Ryan VH, Perdikari TM, Naik MT, Saueressig CF, Lins J, Dignon GL, 
            Mittal J, Hart AC, Fawzi NL
  TITLE     Tyrosine phosphorylation regulates hnRNPA2 granule protein 
            partitioning and reduces neurodegeneration.
  JOURNAL   EMBO J. 2021 Feb 1;40(3):e105001. doi: 10.15252/embj.2020105001. 
            Epub 2020 Dec 22.
  PUBMED    33349959
REFERENCE   2  (bases 1 to 8877)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J."; 
            date: "2021-02-1"; volume: "40(3):e105001. doi"; pages: " 
            10.15252/embj.2020105001. Epub 2020 Dec 22"
COMMENT     Sequence Label: pHA842 - hrp-1p::hrp-1HsLCD290V
FEATURES             Location/Qualifiers
     source          1..8877
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(3639..3655)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3640..3656)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(3685..3705)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(3685..3703)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3724..3740)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3724..3740)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3737..3759)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3748..3764
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3772..3778,3779..3796,3797..3802))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3817..3838
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3955..3972)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4126..4714)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4206..4225)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4885..5676,5677..5745))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5508..5527
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5746..5850)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      5877..6332
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5964..5983)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6174..6195
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6458..6480
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6472..6489
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6473..6489
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6499..6518
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6499..6517
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 attgataacc tgtcaaaacc ggacgaccgg acgccaaata tgacagccct agtcttacca
       61 gtataatatt tttataaacc taaaacaggt atgaatatct cttttttctg cgcagcgctg
      121 ggatgcttct gtttcagaaa aatcacatgg tataaaaaat ttccaaatta cagtagtcct
      181 accgtaatcc tgcattatcc cactactgta tgtaccttga cattattttt cctcgcccaa
      241 ctcccagccc aatatatctc ttcaacagct aagaactaca tttttcctaa actacagtaa
      301 accaacagta tccctacagt accacattaa ttataaatta aaacattatt tttcataaga
      361 aatcgtgaga atttcgagaa aaataaagga ttcaaagatt atttgaacgt ttttttcgtg
      421 aaaaaatgtc aaagaaatca aaatgttcat cttccttgtg aaaaatccga aaagccatta
      481 taaactagtt gattttcata aaaatttgta gaaatatttt tcttaaaatt tgtagaagaa
      541 ttttaacgtt ttttaaacaa aaattccaaa attggaattt ggtattgaaa atacttttta
      601 aaagttattt ttttaatttg taaaaattta actaaattca aaaaataaag tttccctcct
      661 ttttttctta aaggcacaca catttatcga ctcgggtctc gccgcgaaca gacgcgacgc
      721 gacgcggtta ggaatgcggc gcgtcgcgtg taaccctttt ctctgtggtt ttcgagagca
      781 ttctctctcg gttttcgtca caaaaaggta tgttttacag gttttccgcg cactgttttc
      841 gcgtaattct tcattttaat tcagataatc taattgttgt gtagttttag ctcgaattta
      901 tccgtaatct ttgattaaat acgaataaaa ctcgctaaaa tagtcttatt tctcagatat
      961 ttatcgattt gttattttcc acgaatagtc tactcgtgca tggcgtctat atttaactta
     1021 ataatctatt tttagattta tctttactct aacttgtacc tatttcagtg atcaacacct
     1081 ctgtcactct ttgtgagtaa tagcgttcga taggtagtta caccgtgaaa ctagcctgtc
     1141 tatttcctac ttcataagct aagaactact tgttagcaca atccgatcga ctactaagaa
     1201 ctagatcatg acggacgtgg aaatcaaggc tgaggttacg tttgttcact tgcttttagg
     1261 gtgtaggcca cctgatttgg gtcccgatca tttaattatc gattttattt tcagaatgga
     1321 agcggagatg cctcgctcga gccagagaac cttcgaaaga ttttcgttgg aggactcacg
     1381 tcaaacacca ccgatgacct gatgcgagag ttctactcac aattcggaga aatcactgat
     1441 atcatcgtca tgagagatcc aactaccaag agatcccgcg gattcggatt tgtcaccttc
     1501 tctggcaaaa ctgaagtcga tgctgccatg aaacaacgcc cgcacatcat cgacggaaag
     1561 accgtggacc cgaagcgtgc cgtgccacgt gatgataaga accgctccga gtcgaatgtc
     1621 tccaccaaac gcctgtacgt cagcggagtt cgtgaggatc acaccgagga catgctcacc
     1681 gagtatttca caaagtatgg aaccgtcacc aaatccgaga ttattctcga caaagccacc
     1741 caaaagccaa gaggcttcgg attcgtcacc ttcgatgatc atgactctgt ggatcaatgc
     1801 gttcttcaaa aatcccatat ggtcaacgga cacagatgtg acgtgcgcaa aggactctcc
     1861 aaggacgaga tgagcaaggc tcagatgaac cgcgatcgtg aaacccgtgg cggaagatct
     1921 cgcgatggac agcgtggcgg ttacaatggc ggtggtggtg gcggcggcgg ctggggagga
     1981 ccagctcaac gcggcggtcc aggagcatat ggtggaccag gaggtggcgg ccaaggagga
     2041 tatggcggag gtagttggat tctggcttgt gaattctcga ttctaatatt attaactttc
     2101 agattacgga ggaggacgcg gtggttatgg tggtggagga ccaggatatg gaaatcaggg
     2161 tggaggatat ggaggaggat atgataatta tggaggagga aattacggat ctggaaatta
     2221 caatgttttc ggaaactaca accagcagcc atctaattat ggaccaatga agtctggaaa
     2281 tttcggagga tctcgcaata tgggtggacc atatggagga ggaaattatg gtccaggagg
     2341 atctggagga tctggaggat atggaggacg ttctcgttac taaattaatt ccttaagccc
     2401 ctctaagtgt ccaacgtgtc tgagcttccc gtgctctctc ctctgtatcg atcttctcaa
     2461 ccatttttgc tctgctatct catcaaaatc ttctgtactt tttcttgttg cctttctcca
     2521 gaaagtttcg tttattattc aattttttac cctgtctttt ttatataatt tatttctaaa
     2581 ttcgcacagt tgacatccat tcaactagag aatgaatgca gagtttgaca ccaaattggc
     2641 ttaattttca agctgttttt aaccaaaata ataaaaaagt ctcaacttta caaaaatgtt
     2701 tatctggatc tggacgacag cgagctgaga aattgatata ttttcagagt aaaaactaaa
     2761 aatgtctgga atttttggag tcattgtctc agggaggacg ccgatcgaag tggtgccggt
     2821 ctccgatacg gagttcacct gcgaaattgt aaacgctgac gcgataaacc acgtggtggt
     2881 cttccttacc ggagctcaac cgtttccgga cggaattggt ggctctggtg atcattttca
     2941 gaattttccg cccaaaaaaa cccaatttat ttcagtttac atcagatggc ccacccaaga
     3001 cggcggaaat tggcattatt tgggatttat ttgcaacgag aagcccagcg cgatttataa
     3061 agttgctcag gtgtgcattt ttgatggaaa aatgaaagaa aaccaccgaa atttcgatgt
     3121 ttctctgtcg atttttttta tttaaaaact tagaaatact ctcaatttcg catttttcac
     3181 caaaaaaaag aatctcaact ggtttttcac atagcttaat ttttgaagaa aaatctcaac
     3241 aaaacccagt tttaagtttc aaattctcaa aaattgatct tttttttccc ttaaaaatcc
     3301 agtttttagc tccaaattgt cataattttc cagaaaaatt tagttttcga gctaacaaca
     3361 ttaaaatttc acagaaaatc tgaatttcag ccttagagat ccaaattttt cggctgaaaa
     3421 tcacaatttt tccatttttc agctccacaa atccgacgct tctcactcgt tttcccaatt
     3481 tgacaatcaa atgcaactat attccagtgg atcagctcaa attggaatta atgctgaaag
     3541 tttatctgat atcactggac ggcaggcagc tgatgggaca caggctagcc agcaggtgaa
     3601 ggatcccccg ggctgcagga attcgatatc aagcttatcg ataccgtcga cctcgagggg
     3661 gggcccggta cccagctttt gttcccttta gtgagggtta attgcgcgct tggcgtaatc
     3721 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     3781 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
     3841 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     3901 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     3961 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     4021 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     4081 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     4141 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     4201 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     4261 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     4321 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     4381 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     4441 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     4501 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     4561 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     4621 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     4681 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     4741 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     4801 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     4861 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     4921 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     4981 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     5041 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     5101 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     5161 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     5221 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     5281 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     5341 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     5401 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     5461 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     5521 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     5581 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     5641 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     5701 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     5761 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     5821 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgc
     5881 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
     5941 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
     6001 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
     6061 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
     6121 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
     6181 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
     6241 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
     6301 gaattttaac aaaatattaa cgcttacaat ttccattcgc cattcaggct gcgcaactgt
     6361 tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
     6421 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
     6481 acggccagtg agcgcgcgta atacgactca ctatagggcg aattggagct ccaccgcggt
     6541 ggcggccgct tcaattcttc ggagcaggcg taggagctga gcccatcttc aatgcctccg
     6601 attttccaaa attaaacact ttttccatga attcttccat taatttggca attgagccaa
     6661 atgaaaagcc agaagagcca gatggagccg gtgttggagt tggagcagac agagttgaac
     6721 ggagttctgg agcagctggg gttggggtcg gagcagcaag agttgaacgt ggttcagggt
     6781 cggcgaggaa ctgaaaatct aggtttttta ggaaaaattc gcctaaaaac atgccgaaaa
     6841 aatttccgaa ttttagacaa ttctggaaaa aattgtagtg aaattgtcta aaaattttgg
     6901 taaaagtttg cagaaaactg tcagaaaatg ttaatttctt acgtgtttcc ttgcttggga
     6961 cactagaaca gcaccgaata agacaaaaag tacaaaacac agcttcatgt tggaatttat
     7021 gccagaaaaa tagctgtaac tcggctttta tagaatagaa ttggttcctg gtgatgctgg
     7081 agttctatta aacatgagaa caggagtatg aaccgtggga agtggtgttt tggttggaga
     7141 ggtgttctgc acggctcggc aattgtggtg catcagtgtc caccaaacat caagcataac
     7201 ctggatgata aggagacaag ctttggtgca aaagttttca aattttaaag caatttttag
     7261 gactcttcaa agcatgaaac tgtttctcaa ggtttccttc tacttctaac tccttctaga
     7321 tgcatcacgg cctcaaaatg taaaacctca gaaatggtac cagaggttca aaaatgcaaa
     7381 tatcagggac ctgggctaga tcttctcgtt tcgagggctg ggaaactgcc cttccgtgtg
     7441 aaaggggcaa ttgtcaggcc tggagcatga tattgatcgg ttttgtgaga agaccaacaa
     7501 ggtagctaat aaatcgatcc tatattgtat taacacatgt atttgaatca gatctttcat
     7561 ttctcattga ctccctcaaa tttccagcaa gaatggatgc tacaattgta agtttaacca
     7621 caaatcattt ttaattatac ctgaatttct tttcagacca aaaccagaaa agtcgtattc
     7681 atatagtgtt gttcaagaga ttgtcagttg tatgtgccta acaaaaaaaa atctatggca
     7741 ttctaaattt acaatttcag catagtcgct cgccatgtgt caccactctt tcgccaaacc
     7801 atcgatttga tggatccagg cattgagaaa attcatttac tcagttctgc cagtccgtag
     7861 tgtagttttt accggatata gagaattgaa tctagaaaac ctggctgcct ggcaagaagt
     7921 cactggtagt aacaggctga tgagggcagc cttcggtgag ctatccgacg tgatgggcat
     7981 gccgaaactt agactaagaa agttcagttt tgataacaca tatggactgg ttgggttatc
     8041 tgggaaagag aatatgcaat ctgtggatga ctgcttcaag gctatgaact taccacatgc
     8101 gaaggtgctt gaagtgactg gaatcgactt tgtggacaat atttcgatgg tgaagtactt
     8161 tcgcccaggt tatctcgaga aaatccgcat gtattcttgt tattgctcag ctagccagga
     8221 gatgagtgaa gaaattgcaa atctcgagca gtggaagcaa gcgaaacatt ttctgtgcat
     8281 tggtggtaga atgaacattc ctattcaaag atttttccac tttgaccagt tctccatcgg
     8341 tcttcagact ctatcaatcg atgatgctgt tcagataaga gatgtgagtt ggtagttaaa
     8401 gtagcgtttt gtaatgtatt ctatattcca gattctcctc agatcgccca gcttcgagtc
     8461 agcaactatc gatgttcaac tacttgaaaa agaagccatt gagaacgtct ttacgccgaa
     8521 tcacctatta atttatagag gaagagagtt cagaccggtg ggaactgatt ccagttttgt
     8581 gatcaatata acaaaaaaca agcttgaaat ccacaagcac aagggagcta atccaccacg
     8641 tcgtagagac gaacggccaa tccacgagcg tatacgtcaa aggcaagatg gatggtgaag
     8701 tttcaatggt tgctctaata cgtttacatg tctatcaaac ctttttctgt tacttaagta
     8761 aatcgttgtt tcatatttaa ccagtcttac ctacggctgg catatcggac gtccggcgtc
     8821 agaaccttgt gcgtctggtt ttagatgttt gaaaattttg gaaaattatt aaagtct
//
