LOCUS       sequence-274648-        8877 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses hrp-1p::hrp-1HsLCdeltaLC::hrp-1 3'UTR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8877)
  AUTHORS   Ryan VH, Perdikari TM, Naik MT, Saueressig CF, Lins J, Dignon GL, 
            Mittal J, Hart AC, Fawzi NL
  TITLE     Tyrosine phosphorylation regulates hnRNPA2 granule protein 
            partitioning and reduces neurodegeneration.
  JOURNAL   EMBO J. 2021 Feb 1;40(3):e105001. doi: 10.15252/embj.2020105001. 
            Epub 2020 Dec 22.
  PUBMED    33349959
REFERENCE   2  (bases 1 to 8877)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J."; 
            date: "2021-02-1"; volume: "40(3):e105001. doi"; pages: " 
            10.15252/embj.2020105001. Epub 2020 Dec 22"
COMMENT     Sequence Label: pHA843 - hrp-1p::hrp-1HsLCdeltaLC
FEATURES             Location/Qualifiers
     source          1..8877
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     120..142
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     134..151
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     135..151
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     161..180
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        161..179
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6178..6194)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6179..6195)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(6224..6244)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(6224..6242)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6263..6279)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6263..6279)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6276..6298)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6287..6303
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6311..6317,6318..6335,6336..6341))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6356..6377
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6494..6511)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6665..7253)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6745..6764)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7424..8215,8216..8284))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8047..8066
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8285..8389)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      8416..8871
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8503..8522)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8713..8734
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg
       61 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc
      121 ccagtcacga cgttgtaaaa cgacggccag tgagcgcgcg taatacgact cactataggg
      181 cgaattggag ctccaccgcg gtggcggccg cttcaattct tcggagcagg cgtaggagct
      241 gagcccatct tcaatgcctc cgattttcca aaattaaaca ctttttccat gaattcttcc
      301 attaatttgg caattgagcc aaatgaaaag ccagaagagc cagatggagc cggtgttgga
      361 gttggagcag acagagttga acggagttct ggagcagctg gggttggggt cggagcagca
      421 agagttgaac gtggttcagg gtcggcgagg aactgaaaat ctaggttttt taggaaaaat
      481 tcgcctaaaa acatgccgaa aaaatttccg aattttagac aattctggaa aaaattgtag
      541 tgaaattgtc taaaaatttt ggtaaaagtt tgcagaaaac tgtcagaaaa tgttaatttc
      601 ttacgtgttt ccttgcttgg gacactagaa cagcaccgaa taagacaaaa agtacaaaac
      661 acagcttcat gttggaattt atgccagaaa aatagctgta actcggcttt tatagaatag
      721 aattggttcc tggtgatgct ggagttctat taaacatgag aacaggagta tgaaccgtgg
      781 gaagtggtgt tttggttgga gaggtgttct gcacggctcg gcaattgtgg tgcatcagtg
      841 tccaccaaac atcaagcata acctggatga taaggagaca agctttggtg caaaagtttt
      901 caaattttaa agcaattttt aggactcttc aaagcatgaa actgtttctc aaggtttcct
      961 tctacttcta actccttcta gatgcatcac ggcctcaaaa tgtaaaacct cagaaatggt
     1021 accagaggtt caaaaatgca aatatcaggg acctgggcta gatcttctcg tttcgagggc
     1081 tgggaaactg cccttccgtg tgaaaggggc aattgtcagg cctggagcat gatattgatc
     1141 ggttttgtga gaagaccaac aaggtagcta ataaatcgat cctatattgt attaacacat
     1201 gtatttgaat cagatctttc atttctcatt gactccctca aatttccagc aagaatggat
     1261 gctacaattg taagtttaac cacaaatcat ttttaattat acctgaattt cttttcagac
     1321 caaaaccaga aaagtcgtat tcatatagtg ttgttcaaga gattgtcagt tgtatgtgcc
     1381 taacaaaaaa aaatctatgg cattctaaat ttacaatttc agcatagtcg ctcgccatgt
     1441 gtcaccactc tttcgccaaa ccatcgattt gatggatcca ggcattgaga aaattcattt
     1501 actcagttct gccagtccgt agtgtagttt ttaccggata tagagaattg aatctagaaa
     1561 acctggctgc ctggcaagaa gtcactggta gtaacaggct gatgagggca gccttcggtg
     1621 agctatccga cgtgatgggc atgccgaaac ttagactaag aaagttcagt tttgataaca
     1681 catatggact ggttgggtta tctgggaaag agaatatgca atctgtggat gactgcttca
     1741 aggctatgaa cttaccacat gcgaaggtgc ttgaagtgac tggaatcgac tttgtggaca
     1801 atatttcgat ggtgaagtac tttcgcccag gttatctcga gaaaatccgc atgtattctt
     1861 gttattgctc agctagccag gagatgagtg aagaaattgc aaatctcgag cagtggaagc
     1921 aagcgaaaca ttttctgtgc attggtggta gaatgaacat tcctattcaa agatttttcc
     1981 actttgacca gttctccatc ggtcttcaga ctctatcaat cgatgatgct gttcagataa
     2041 gagatgtgag ttggtagtta aagtagcgtt ttgtaatgta ttctatattc cagattctcc
     2101 tcagatcgcc cagcttcgag tcagcaacta tcgatgttca actacttgaa aaagaagcca
     2161 ttgagaacgt ctttacgccg aatcacctat taatttatag aggaagagag ttcagaccgg
     2221 tgggaactga ttccagtttt gtgatcaata taacaaaaaa caagcttgaa atccacaagc
     2281 acaagggagc taatccacca cgtcgtagag acgaacggcc aatccacgag cgtatacgtc
     2341 aaaggcaaga tggatggtga agtttcaatg gttgctctaa tacgtttaca tgtctatcaa
     2401 acctttttct gttacttaag taaatcgttg tttcatattt aaccagtctt acctacggct
     2461 ggcatatcgg acgtccggcg tcagaacctt gtgcgtctgg ttttagatgt ttgaaaattt
     2521 tggaaaatta ttaaagtcta ttgataacct gtcaaaaccg gacgaccgga cgccaaatat
     2581 gacagcccta gtcttaccag tataatattt ttataaacct aaaacaggta tgaatatctc
     2641 ttttttctgc gcagcgctgg gatgcttctg tttcagaaaa atcacatggt ataaaaaatt
     2701 tccaaattac agtagtccta ccgtaatcct gcattatccc actactgtat gtaccttgac
     2761 attatttttc ctcgcccaac tcccagccca atatatctct tcaacagcta agaactacat
     2821 ttttcctaaa ctacagtaaa ccaacagtat ccctacagta ccacattaat tataaattaa
     2881 aacattattt ttcataagaa atcgtgagaa tttcgagaaa aataaaggat tcaaagatta
     2941 tttgaacgtt tttttcgtga aaaaatgtca aagaaatcaa aatgttcatc ttccttgtga
     3001 aaaatccgaa aagccattat aaactagttg attttcataa aaatttgtag aaatattttt
     3061 cttaaaattt gtagaagaat tttaacgttt tttaaacaaa aattccaaaa ttggaatttg
     3121 gtattgaaaa tactttttaa aagttatttt tttaatttgt aaaaatttaa ctaaattcaa
     3181 aaaataaagt ttccctcctt tttttcttaa aggcacacac atttatcgac tcgggtctcg
     3241 ccgcgaacag acgcgacgcg acgcggttag gaatgcggcg cgtcgcgtgt aacccttttc
     3301 tctgtggttt tcgagagcat tctctctcgg ttttcgtcac aaaaaggtat gttttacagg
     3361 ttttccgcgc actgttttcg cgtaattctt cattttaatt cagataatct aattgttgtg
     3421 tagttttagc tcgaatttat ccgtaatctt tgattaaata cgaataaaac tcgctaaaat
     3481 agtcttattt ctcagatatt tatcgatttg ttattttcca cgaatagtct actcgtgcat
     3541 ggcgtctata tttaacttaa taatctattt ttagatttat ctttactcta acttgtacct
     3601 atttcagtga tcaacacctc tgtcactctt tgtgagtaat agcgttcgat aggtagttac
     3661 accgtgaaac tagcctgtct atttcctact tcataagcta agaactactt gttagcacaa
     3721 tccgatcgac tactaagaac tagatcatga cggacgtgga aatcaaggct gaggttacgt
     3781 ttgttcactt gcttttaggg tgtaggccac ctgatttggg tcccgatcat ttaattatcg
     3841 attttatttt cagaatggaa gcggagatgc ctcgctcgag ccagagaacc ttcgaaagat
     3901 tttcgttgga ggactcacgt caaacaccac cgatgacctg atgcgagagt tctactcaca
     3961 attcggagaa atcactgata tcatcgtcat gagagatcca actaccaaga gatcccgcgg
     4021 attcggattt gtcaccttct ctggcaaaac tgaagtcgat gctgccatga aacaacgccc
     4081 gcacatcatc gacggaaaga ccgtggaccc gaagcgtgcc gtgccacgtg atgataagaa
     4141 ccgctccgag tcgaatgtct ccaccaaacg cctgtacgtc agcggagttc gtgaggatca
     4201 caccgaggac atgctcaccg agtatttcac aaagtatgga accgtcacca aatccgagat
     4261 tattctcgac aaagccaccc aaaagccaag aggcttcgga ttcgtcacct tcgatgatca
     4321 tgactctgtg gatcaatgcg ttcttcaaaa atcccatatg gtcaacggac acagatgtga
     4381 cgtgcgcaaa ggactctcca aggacgagat gagcaaggct cagatgaacc gcgatcgtga
     4441 aacccgtggc ggaagatctc gcgatggaca gcgtggcggt tacaatggcg gtggtggtgg
     4501 cggcggcggc tggggaggac cagctcaacg cggcggtcca ggagcatatg gtggaccagg
     4561 aggtggcggc caaggaggat atggcggagg tagttggatt ctggcttgtg aattctcgat
     4621 tctaatatta ttaactttca gattaatgag gaggaagagg aggatatggt ggtggaggac
     4681 ctggatatgg caaccagggt gggggctacg gaggtggtta tgacaactat ggaggaggaa
     4741 attatggaag tggaaattac aatgattttg gaaattataa ccagcaacct tctaactacg
     4801 gtccaatgaa gagtggaaac tttggtggta gcaggaacat ggggggacca tatggtggag
     4861 gaaactatgg tccaggaggc agtggaggaa gtgggggtta tggtgggagg agccgatact
     4921 aaattaattc cttaagcccc tctaagtgtc caacgtgtct gagcttcccg tgctctctcc
     4981 tctgtatcga tcttctcaac catttttgct ctgctatctc atcaaaatct tctgtacttt
     5041 ttcttgttgc ctttctccag aaagtttcgt ttattattca attttttacc ctgtcttttt
     5101 tatataattt atttctaaat tcgcacagtt gacatccatt caactagaga atgaatgcag
     5161 agtttgacac caaattggct taattttcaa gctgttttta accaaaataa taaaaaagtc
     5221 tcaactttac aaaaatgttt atctggatct ggacgacagc gagctgagaa attgatatat
     5281 tttcagagta aaaactaaaa atgtctggaa tttttggagt cattgtctca gggaggacgc
     5341 cgatcgaagt ggtgccggtc tccgatacgg agttcacctg cgaaattgta aacgctgacg
     5401 cgataaacca cgtggtggtc ttccttaccg gagctcaacc gtttccggac ggaattggtg
     5461 gctctggtga tcattttcag aattttccgc ccaaaaaaac ccaatttatt tcagtttaca
     5521 tcagatggcc cacccaagac ggcggaaatt ggcattattt gggatttatt tgcaacgaga
     5581 agcccagcgc gatttataaa gttgctcagg tgtgcatttt tgatggaaaa atgaaagaaa
     5641 accaccgaaa tttcgatgtt tctctgtcga ttttttttat ttaaaaactt agaaatactc
     5701 tcaatttcgc atttttcacc aaaaaaaaga atctcaactg gtttttcaca tagcttaatt
     5761 tttgaagaaa aatctcaaca aaacccagtt ttaagtttca aattctcaaa aattgatctt
     5821 ttttttccct taaaaatcca gtttttagct ccaaattgtc ataattttcc agaaaaattt
     5881 agttttcgag ctaacaacat taaaatttca cagaaaatct gaatttcagc cttagagatc
     5941 caaatttttc ggctgaaaat cacaattttt ccatttttca gctccacaaa tccgacgctt
     6001 ctcactcgtt ttcccaattt gacaatcaaa tgcaactata ttccagtgga tcagctcaaa
     6061 ttggaattaa tgctgaaagt ttatctgata tcactggacg gcaggcagct gatgggacac
     6121 aggctagcca gcaggtgaag gatcccccgg gctgcaggaa ttcgatatca agcttatcga
     6181 taccgtcgac ctcgaggggg ggcccggtac ccagcttttg ttccctttag tgagggttaa
     6241 ttgcgcgctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
     6301 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
     6361 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
     6421 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     6481 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     6541 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     6601 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     6661 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     6721 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     6781 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     6841 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     6901 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     6961 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     7021 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     7081 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     7141 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     7201 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     7261 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     7321 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     7381 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     7441 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     7501 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     7561 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     7621 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     7681 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     7741 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     7801 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     7861 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     7921 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     7981 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     8041 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     8101 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     8161 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     8221 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     8281 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     8341 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     8401 gaaaagtgcc acctgacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
     8461 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
     8521 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
     8581 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
     8641 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
     8701 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
     8761 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
     8821 tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt tccattc
//
