LOCUS       sequence-27466-e         633 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 633)
  AUTHORS   Liu Y, Henry GD, Hegde RS, Baleja JD
  TITLE     Solution structure of the hDlg/SAP97 PDZ2 domain and its mechanism 
            of interaction with HPV-18 papillomavirus E6 protein.
  JOURNAL   Biochemistry. 2007 Sep 25. 46(38):10864-74.
  PUBMED    17713926
REFERENCE   2  (bases 1 to 633)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry. 2007 Sep 25. 46(38):10864-74."
COMMENT     Sequence Label: pET30-hDlg PDZ2
FEATURES             Location/Qualifiers
     source          1..633
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(23..40)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(84..101)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             382..404
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    419..443
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(443..462)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(444..462)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(550..569)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 ctttgttagc agccggatct cagtggtggt ggtggtggtg ctcgagtgcg gccgcaagct
       61 tgtcgacgga gctcgaattc ttagtggtgg tggtggtggt gggatcctgt gggttttgcc
      121 actttcaaat aaacaaaatc agatgtgttc tttaaggcag ttactgcttc ttcatgagta
      181 acttcttcta aacatacgtt attcactgct aaaagtttat ctccaatctg aagtttgcca
      241 tccttatgtg ctgcacctcc ttcaattatt ttggttacat agatgctatt atccccagga
      301 atatgctgat ttccaacacc tccagcaatg ctaaacccaa gacctttagg acctttaatg
      361 agctttattt ccatatgtat atctccttct taaagttaaa caaaattatt tctagagggg
      421 aattgttatc cgctcacaat tcccctatag tgagtcgtat taatttcgcg ggatcgagat
      481 cgatctcgat cctctacgcc ggacgcatcg tggccggcat caccggcgcc acaggtgcgg
      541 ttgctggcgc ctatatcgcc gacatcaccg atggggaaga tcgggctcgc cacttcgggc
      601 tcatgagcgc ttgtttcggc gtgggtatgg tgg
//
