LOCUS       sequence-27468-e         686 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 686)
  AUTHORS   Liu Y, Cherry JJ, Dineen JV, Androphy EJ, Baleja JD
  TITLE     Determinants of stability for the E6 protein of papillomavirus type 
            16.
  JOURNAL   J Mol Biol. 2009 Mar 6. 386(4):1123-37.
  PUBMED    19244625
REFERENCE   2  (bases 1 to 686)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Mol Biol.
            2009 Mar 6. 386(4):1123-37."
COMMENT     Sequence Label: pET30-GBF-E6C
FEATURES             Location/Qualifiers
     source          1..686
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             12..34
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             439..456
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(509..527)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      523..570
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 tctagaataa ttttgtttaa ctttaagaag gagatataca tatgcagtac aaactgatcc
       61 tgaacggtaa aaccctgaaa ggtgaaacca ccaccgaagc tgttaacgct gctaccgcgg
      121 aaaaagtttt caaacagtac gctaaccgta acggtgttga cggtaaatgg acctacgacg
      181 acgctaccaa aaccttcacc gttaccgaag ctgctcccag aggatcctat agtttgtatg
      241 gaacaacatt agaacagcaa tacaacaaac cgttgagtga tttgttaatt aggtgtatta
      301 actgtcaaaa gccactgagt cctgaagaaa agcaaagaca tctggacaaa aagcaaagat
      361 tccataatat aaggggtcgg tggaccggtc gatgtatgtc ttgtagcaga agctagtaag
      421 cttgcggccg cactcgagca ccaccaccac caccactgag atccggctgc taacaaagcc
      481 cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg
      541 gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggattggcg
      601 aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
      661 tgaccgctac acttgccagc gcccta
//
