LOCUS       sequence-27469-e         653 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 653)
  AUTHORS   Liu Y, Cherry JJ, Dineen JV, Androphy EJ, Baleja JD
  TITLE     Determinants of stability for the E6 protein of papillomavirus type 
            16.
  JOURNAL   J Mol Biol. 2009 Mar 6. 386(4):1123-37.
  PUBMED    19244625
REFERENCE   2  (bases 1 to 653)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Mol Biol.
            2009 Mar 6. 386(4):1123-37."
COMMENT     Sequence Label: pET30-GBF-E6N
FEATURES             Location/Qualifiers
     source          1..653
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             12..34
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 tctagaataa ttttgtttaa ctttaagaag gagatataca tatgcagtac aaactgatcc
       61 tgaacggtaa aaccctgaaa ggtgaaacca ccaccgaagc tgttaacgct gctaccgcgg
      121 aaaaagtttt caaacagtac gctaaccgta acggtgttga cggtaaatgg acctacgacg
      181 acgctaccaa aaccttcacc gttaccgaag ctgctcccag aggatccttt caggacccac
      241 aggagcgacc cagaaagtta ccacagttat gcacagagct gcaaacaact atacatgata
      301 taatattaga atgtgtgtac tgcaagcaac agttactgcg acgtgaggta tatgactttg
      361 cttttcggga tttatgcata gtatatagag atgggaatcc atatgctgta tgtgataaat
      421 gtttaaagtt ttattctaaa attagtgagt gaagacatta tagttatagt ttgtatggaa
      481 caacattaga acagcaatac aacaaaccgt tgagtgattt gttaattagg tgtattaact
      541 gtcaaaagcc actgagtcct gaagaaaagc aaagacatct ggacaaaaag caaagattcc
      601 ataatataag gggtcggtgg accggtcgat gtatgtcttg tagcagaagc tag
//
