LOCUS       sequence-274713-        7168 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses mScarlet tagged hrp-1HsLCD290V in mec-4 neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7168)
  AUTHORS   Ryan VH, Perdikari TM, Naik MT, Saueressig CF, Lins J, Dignon GL, 
            Mittal J, Hart AC, Fawzi NL
  TITLE     Tyrosine phosphorylation regulates hnRNPA2 granule protein 
            partitioning and reduces neurodegeneration.
  JOURNAL   EMBO J. 2021 Feb 1;40(3):e105001. doi: 10.15252/embj.2020105001. 
            Epub 2020 Dec 22.
  PUBMED    33349959
REFERENCE   2  (bases 1 to 7168)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J."; 
            date: "2021-02-1"; volume: "40(3):e105001. doi"; pages: " 
            10.15252/embj.2020105001. Epub 2020 Dec 22"
COMMENT     Sequence Label: pHA849 - mec-4p::hrp-1HsLCD290VmScarlet
FEATURES             Location/Qualifiers
     source          1..7168
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(42..833,834..902))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     665..684
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(903..1007)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      1034..1489
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1121..1140)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1331..1352
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1615..1637
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1629..1646
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1630..1646
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1656..1675
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1656..1674
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3556..4251
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016)"
                     /label=mScarlet
                     /translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYYKQSFPEGFKWERVMNFEDGG
                     AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
                     MALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH
                     STGGMDELYK"
     primer_bind     complement(3689..3707)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3957..3976
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(5964..5980)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5965..5981)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(6010..6030)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(6010..6028)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6049..6065)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6049..6065)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6062..6084)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6073..6089
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6097..6103,6104..6121,6122..6127))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6142..6163
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6280..6297)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6451..7039)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6531..6550)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
       61 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
      121 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
      181 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
      241 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
      301 gaagctagag taagtagttc accagttaat agtttgcgca acgttgttgc cattgctaca
      361 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
      421 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
      481 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
      541 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
      601 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
      661 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
      721 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
      781 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
      841 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
      901 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
      961 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     1021 aaagtgccac ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     1081 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     1141 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     1201 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     1261 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     1321 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
     1381 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
     1441 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaatttc cattcgccat
     1501 tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc
     1561 tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt
     1621 cacgacgttg taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat
     1681 tggagctcca ccgcggtggc ggccgctaag cttcaataca agctcaaata caatactatt
     1741 ttttttccgt ctgaacataa aattagtttc taaataaaaa atgtgaaaat aaatatttgg
     1801 ttttaatcgt taaaatcttc caaattacta ataaagttca gtcaactata tgcttcgaaa
     1861 ttcactgaat ctattattgt tattaatttt tagacccttg gagagctccc aactctttca
     1921 atccagaaga ctgatggatg ccaggtgtac ttgtcaaagg tggctcaagg atgcgagatt
     1981 gtcacttcca aatccagtga gatgaacatc tccgtgcaaa ccaatgatga tggggattat
     2041 gtaagttttt cggattgtag aacccaatct aaatttcctt ttcagtcgga gttcccggtt
     2101 ccggagcagt tcaagacaac ttttgtcaat ggcaagctag tcactgtcgt ctcggacatt
     2161 tgctaaatct tctcacgtca taaccttatt tttcaacttc atgaactatc ccctcgaact
     2221 tttttatttg tccctgtcta caattcttta taagcaattg agacacactt tgctttatta
     2281 aataataccg attttgtttt gtatatgcct tgactggtgc tcaactattt ttattgaaaa
     2341 aattactttt tactgtctcg tcgaataaaa gtgatccctt ccaaaaaata gttattcatg
     2401 tttgaaggtt tgtgatcgta gaacaattgc accgaatgta gtgtaaaaaa aattccagaa
     2461 tgttcaaata catttccacc ctcaccagaa catctcaaac accaggcaca tttcaacaca
     2521 ctttcatgga tcttatcttg cctcaaaacc atcagctttc ttctcattct acaagtatga
     2581 aacaatctac tattctatac atttataatg taatggaacc atgtgaagac atgagcaaga
     2641 tcattgtcgt cagcgactac ccctccttca tttttcttag agtatcagtt tgctaatgct
     2701 atttttttta tcgctatcaa gttatagagg gatccccggg attggccaaa ggacccaaag
     2761 gtatgtttcg aatgatacta acataacata gaacattttc aggaggaccc ttgcttggag
     2821 ggtaccggta gaaaaaatga cggacgtgga aatcaaggct gaggttacgt ttgttcactt
     2881 gcttttaggg tgtaggccac ctgatttggg tcccgatcat ttaattatcg attttatttt
     2941 cagaatggaa gcggagatgc ctcgctcgag ccagagaacc ttcgaaagat tttcgttgga
     3001 ggactcacgt caaacaccac cgatgacctg atgcgagagt tctactcaca attcggagaa
     3061 atcactgata tcatcgtcat gagagatcca actaccaaga gatcccgcgg attcggattt
     3121 gtcaccttct ctggcaaaac tgaagtcgat gctgccatga aacaacgccc gcacatcatc
     3181 gacggaaaga ccgtggaccc gaagcgtgcc gtgccacgtg atgataagaa ccgctccgag
     3241 tcgaatgtct ccaccaaacg cctgtacgtc agcggagttc gtgaggatca caccgaggac
     3301 atgctcaccg agtatttcac aaagtatgga accgtcacca aatccgagat tattctcgac
     3361 aaagccaccc aaaagccaag aggcttcgga ttcgtcacct tcgatgatca tgactctgtg
     3421 gatcaatgcg ttcttcaaaa atcccatatg gtcaacggac acagatgtga cgtgcgcaaa
     3481 ggactctcca aggacgagat gagcaaggct cagatgaacc gcgatcgtga aacccgtggc
     3541 ggaagatctc gcgatatggt gagcaagggc gaggcagtga tcaaggagtt catgcggttc
     3601 aaggtgcaca tggagggctc catgaacggc cacgagttcg agatcgaggg cgagggcgag
     3661 ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg tggccccctg
     3721 cccttctcct gggacatcct gtcccctcag ttcatgtacg gctccagggc cttcaccaag
     3781 caccccgccg acatccccga ctactataag cagtccttcc ccgagggctt caagtgggag
     3841 cgcgtgatga acttcgagga cggcggcgcc gtgaccgtga cccaggacac ctccctggag
     3901 gacggcaccc tgatctacaa ggtgaagctc cgcggcacca acttccctcc tgacggcccc
     3961 gtaatgcaga agaagacaat gggctgggaa gcgtccaccg agcggttgta ccccgaggac
     4021 ggcgtgctga agggcgacat taagatggcc ctgcgcctga aggacggcgg ccgctacctg
     4081 gcggacttca agaccaccta caaggccaag aagcccgtgc agatgcccgg cgcctacaac
     4141 gtcgaccgca agttggacat cacctcccac aacgaggact acaccgtggt ggaacagtac
     4201 gaacgctccg agggccgcca ctccaccggc ggcatggacg agctgtacaa gggacagcgt
     4261 ggcggttaca atggcggtgg tggtggcggc ggcggctggg gaggaccagc tcaacgcggc
     4321 ggtccaggag catatggtgg accaggaggt ggcggccaag gaggatatgg cggaggtagt
     4381 tggattctgg cttgtgaatt ctcgattcta atattattaa ctttcagatt acggaggagg
     4441 acgcggtggt tatggtggtg gaggaccagg atatggaaat cagggtggag gatatggagg
     4501 aggatatgat aattatggag gaggaaatta cggatctgga aattacaatg ttttcggaaa
     4561 ctacaaccag cagccatcta attatggacc aatgaagtct ggaaatttcg gaggatctcg
     4621 caatatgggt ggaccatatg gaggaggaaa ttatggtcca ggaggatctg gaggatctgg
     4681 aggatatgga ggacgttctc gttactaaat taattcctta agcccctcta agtgtccaac
     4741 gtgtctgagc ttcccgtgct ctctcctctg tatcgatctt ctcaaccatt tttgctctgc
     4801 tatctcatca aaatcttctg tactttttct tgttgccttt ctccagaaag tttcgtttat
     4861 tattcaattt tttaccctgt cttttttata taatttattt ctaaattcgc acagttgaca
     4921 tccattcaac tagagaatga atgcagagtt tgacaccaaa ttggcttaat tttcaagctg
     4981 tttttaacca aaataataaa aaagtctcaa ctttacaaaa atgtttatct ggatctggac
     5041 gacagcgagc tgagaaattg atatattttc agagtaaaaa ctaaaaatgt ctggaatttt
     5101 tggagtcatt gtctcaggga ggacgccgat cgaagtggtg ccggtctccg atacggagtt
     5161 cacctgcgaa attgtaaacg ctgacgcgat aaaccacgtg gtggtcttcc ttaccggagc
     5221 tcaaccgttt ccggacggaa ttggtggctc tggtgatcat tttcagaatt ttccgcccaa
     5281 aaaaacccaa tttatttcag tttacatcag atggcccacc caagacggcg gaaattggca
     5341 ttatttggga tttatttgca acgagaagcc cagcgcgatt tataaagttg ctcaggtgtg
     5401 catttttgat ggaaaaatga aagaaaacca ccgaaatttc gatgtttctc tgtcgatttt
     5461 ttttatttaa aaacttagaa atactctcaa tttcgcattt ttcaccaaaa aaaagaatct
     5521 caactggttt ttcacatagc ttaatttttg aagaaaaatc tcaacaaaac ccagttttaa
     5581 gtttcaaatt ctcaaaaatt gatctttttt ttcccttaaa aatccagttt ttagctccaa
     5641 attgtcataa ttttccagaa aaatttagtt ttcgagctaa caacattaaa atttcacaga
     5701 aaatctgaat ttcagcctta gagatccaaa tttttcggct gaaaatcaca atttttccat
     5761 ttttcagctc cacaaatccg acgcttctca ctcgttttcc caatttgaca atcaaatgca
     5821 actatattcc agtggatcag ctcaaattgg aattaatgct gaaagtttat ctgatatcac
     5881 tggacggcag gcagctgatg ggacacaggc tagccagcag gtgaaggatc ccccgggctg
     5941 caggaattcg atatcaagct tatcgatacc gtcgacctcg agggggggcc cggtacccag
     6001 cttttgttcc ctttagtgag ggttaattgc gcgcttggcg taatcatggt catagctgtt
     6061 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     6121 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     6181 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     6241 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     6301 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     6361 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     6421 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     6481 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     6541 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     6601 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     6661 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     6721 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     6781 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     6841 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     6901 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     6961 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     7021 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     7081 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     7141 gatcctttta aattaaaaat gaagtttt
//
