LOCUS       sequence-274714-        9570 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses mScarlet tagged hrp-1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9570)
  AUTHORS   Ryan VH, Perdikari TM, Naik MT, Saueressig CF, Lins J, Dignon GL, 
            Mittal J, Hart AC, Fawzi NL
  TITLE     Tyrosine phosphorylation regulates hnRNPA2 granule protein 
            partitioning and reduces neurodegeneration.
  JOURNAL   EMBO J. 2021 Feb 1;40(3):e105001. doi: 10.15252/embj.2020105001. 
            Epub 2020 Dec 22.
  PUBMED    33349959
REFERENCE   2  (bases 1 to 9570)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J."; 
            date: "2021-02-1"; volume: "40(3):e105001. doi"; pages: " 
            10.15252/embj.2020105001. Epub 2020 Dec 22"
COMMENT     Sequence Label: pHA844 - hrp-1p::hrp-1mScarlet
FEATURES             Location/Qualifiers
     source          1..9570
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1557..2252
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016)"
                     /label=mScarlet
                     /translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYYKQSFPEGFKWERVMNFEDGG
                     AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
                     MALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH
                     STGGMDELYK"
     primer_bind     complement(1690..1708)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1958..1977
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(3962..3978)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3963..3979)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(4008..4028)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(4008..4026)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4047..4063)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4047..4063)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4060..4082)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4071..4087
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4095..4101,4102..4119,4120..4125))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4140..4161
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4278..4295)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4449..5037)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4529..4548)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5208..5999,6000..6068))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5831..5850
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6069..6173)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      6200..6655
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6287..6306)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6497..6518
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6781..6803
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6795..6812
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6796..6812
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6822..6841
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6822..6840
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 atttcgagaa aaataaagga ttcaaagatt atttgaacgt ttttttcgtg aaaaaatgtc
       61 aaagaaatca aaatgttcat cttccttgtg aaaaatccga aaagccatta taaactagtt
      121 gattttcata aaaatttgta gaaatatttt tcttaaaatt tgtagaagaa ttttaacgtt
      181 ttttaaacaa aaattccaaa attggaattt ggtattgaaa atacttttta aaagttattt
      241 ttttaatttg taaaaattta actaaattca aaaaataaag tttccctcct ttttttctta
      301 aaggcacaca catttatcga ctcgggtctc gccgcgaaca gacgcgacgc gacgcggtta
      361 ggaatgcggc gcgtcgcgtg taaccctttt ctctgtggtt ttcgagagca ttctctctcg
      421 gttttcgtca caaaaaggta tgttttacag gttttccgcg cactgttttc gcgtaattct
      481 tcattttaat tcagataatc taattgttgt gtagttttag ctcgaattta tccgtaatct
      541 ttgattaaat acgaataaaa ctcgctaaaa tagtcttatt tctcagatat ttatcgattt
      601 gttattttcc acgaatagtc tactcgtgca tggcgtctat atttaactta ataatctatt
      661 tttagattta tctttactct aacttgtacc tatttcagtg atcaacacct ctgtcactct
      721 ttgtgagtaa tagcgttcga taggtagtta caccgtgaaa ctagcctgtc tatttcctac
      781 ttcataagct aagaactact tgttagcaca atccgatcga ctactaagaa ctagatcatg
      841 acggacgtgg aaatcaaggc tgaggttacg tttgttcact tgcttttagg gtgtaggcca
      901 cctgatttgg gtcccgatca tttaattatc gattttattt tcagaatgga agcggagatg
      961 cctcgctcga gccagagaac cttcgaaaga ttttcgttgg aggactcacg tcaaacacca
     1021 ccgatgacct gatgcgagag ttctactcac aattcggaga aatcactgat atcatcgtca
     1081 tgagagatcc aactaccaag agatcccgcg gattcggatt tgtcaccttc tctggcaaaa
     1141 ctgaagtcga tgctgccatg aaacaacgcc cgcacatcat cgacggaaag accgtggacc
     1201 cgaagcgtgc cgtgccacgt gatgataaga accgctccga gtcgaatgtc tccaccaaac
     1261 gcctgtacgt cagcggagtt cgtgaggatc acaccgagga catgctcacc gagtatttca
     1321 caaagtatgg aaccgtcacc aaatccgaga ttattctcga caaagccacc caaaagccaa
     1381 gaggcttcgg attcgtcacc ttcgatgatc atgactctgt ggatcaatgc gttcttcaaa
     1441 aatcccatat ggtcaacgga cacagatgtg acgtgcgcaa aggactctcc aaggacgaga
     1501 tgagcaaggc tcagatgaac cgcgatcgtg aaacccgtgg cggaagatct cgcgatatgg
     1561 tgagcaaggg cgaggcagtg atcaaggagt tcatgcggtt caaggtgcac atggagggct
     1621 ccatgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca
     1681 cccagaccgc caagctgaag gtgaccaagg gtggccccct gcccttctcc tgggacatcc
     1741 tgtcccctca gttcatgtac ggctccaggg ccttcaccaa gcaccccgcc gacatccccg
     1801 actactataa gcagtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg
     1861 acggcggcgc cgtgaccgtg acccaggaca cctccctgga ggacggcacc ctgatctaca
     1921 aggtgaagct ccgcggcacc aacttccctc ctgacggccc cgtaatgcag aagaagacaa
     1981 tgggctggga agcgtccacc gagcggttgt accccgagga cggcgtgctg aagggcgaca
     2041 ttaagatggc cctgcgcctg aaggacggcg gccgctacct ggcggacttc aagaccacct
     2101 acaaggccaa gaagcccgtg cagatgcccg gcgcctacaa cgtcgaccgc aagttggaca
     2161 tcacctccca caacgaggac tacaccgtgg tggaacagta cgaacgctcc gagggccgcc
     2221 actccaccgg cggcatggac gagctgtaca agggacagcg tggcggttac aatggcggtg
     2281 gtggtggcgg cggcggctgg ggaggaccag ctcaacgcgg cggtccagga gcatatggtg
     2341 gaccaggagg tggcggccaa ggaggatatg gcggaggtag ttggattctg gcttgtgaat
     2401 tctcgattct aatattatta actttcagat tacggcggtg gctggggaca gcaaggaggc
     2461 ggcggtcaag gcggatgggg aggcccacaa cagcagcaag gaggtggtgg ctggggacag
     2521 caaggcggcg gcggtcaagg cggatgggga ggaccacagc aacagcagca aggaggctgg
     2581 ggaggtcctc aacaaggagg cggcggcggt ggctggggag gccaaggcca gcagcaaggc
     2641 ggctggggag gtcaatccgg tgctcagcag tgggctcatg ctcaaggagg aaacagaaac
     2701 tattaaatta attccttaag cccctctaag tgtccaacgt gtctgagctt cccgtgctct
     2761 ctcctctgta tcgatcttct caaccatttt tgctctgcta tctcatcaaa atcttctgta
     2821 ctttttcttg ttgcctttct ccagaaagtt tcgtttatta ttcaattttt taccctgtct
     2881 tttttatata atttatttct aaattcgcac agttgacatc cattcaacta gagaatgaat
     2941 gcagagtttg acaccaaatt ggcttaattt tcaagctgtt tttaaccaaa ataataaaaa
     3001 agtctcaact ttacaaaaat gtttatctgg atctggacga cagcgagctg agaaattgat
     3061 atattttcag agtaaaaact aaaaatgtct ggaatttttg gagtcattgt ctcagggagg
     3121 acgccgatcg aagtggtgcc ggtctccgat acggagttca cctgcgaaat tgtaaacgct
     3181 gacgcgataa accacgtggt ggtcttcctt accggagctc aaccgtttcc ggacggaatt
     3241 ggtggctctg gtgatcattt tcagaatttt ccgcccaaaa aaacccaatt tatttcagtt
     3301 tacatcagat ggcccaccca agacggcgga aattggcatt atttgggatt tatttgcaac
     3361 gagaagccca gcgcgattta taaagttgct caggtgtgca tttttgatgg aaaaatgaaa
     3421 gaaaaccacc gaaatttcga tgtttctctg tcgatttttt ttatttaaaa acttagaaat
     3481 actctcaatt tcgcattttt caccaaaaaa aagaatctca actggttttt cacatagctt
     3541 aatttttgaa gaaaaatctc aacaaaaccc agttttaagt ttcaaattct caaaaattga
     3601 tctttttttt cccttaaaaa tccagttttt agctccaaat tgtcataatt ttccagaaaa
     3661 atttagtttt cgagctaaca acattaaaat ttcacagaaa atctgaattt cagccttaga
     3721 gatccaaatt tttcggctga aaatcacaat ttttccattt ttcagctcca caaatccgac
     3781 gcttctcact cgttttccca atttgacaat caaatgcaac tatattccag tggatcagct
     3841 caaattggaa ttaatgctga aagtttatct gatatcactg gacggcaggc agctgatggg
     3901 acacaggcta gccagcaggt gaaggatccc ccgggctgca ggaattcgat atcaagctta
     3961 tcgataccgt cgacctcgag ggggggcccg gtacccagct tttgttccct ttagtgaggg
     4021 ttaattgcgc gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
     4081 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
     4141 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     4201 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     4261 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     4321 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     4381 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     4441 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     4501 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     4561 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     4621 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     4681 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     4741 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     4801 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     4861 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     4921 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     4981 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     5041 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     5101 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     5161 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     5221 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     5281 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     5341 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     5401 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     5461 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     5521 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     5581 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     5641 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     5701 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     5761 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     5821 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     5881 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     5941 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     6001 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     6061 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     6121 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     6181 ccccgaaaag tgccacctga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     6241 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     6301 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     6361 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     6421 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     6481 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     6541 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
     6601 ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttccatt
     6661 cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac
     6721 gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt
     6781 cccagtcacg acgttgtaaa acgacggcca gtgagcgcgc gtaatacgac tcactatagg
     6841 gcgaattgga gctccaccgc ggtggcggcc gcttcaattc ttcggagcag gcgtaggagc
     6901 tgagcccatc ttcaatgcct ccgattttcc aaaattaaac actttttcca tgaattcttc
     6961 cattaatttg gcaattgagc caaatgaaaa gccagaagag ccagatggag ccggtgttgg
     7021 agttggagca gacagagttg aacggagttc tggagcagct ggggttgggg tcggagcagc
     7081 aagagttgaa cgtggttcag ggtcggcgag gaactgaaaa tctaggtttt ttaggaaaaa
     7141 ttcgcctaaa aacatgccga aaaaatttcc gaattttaga caattctgga aaaaattgta
     7201 gtgaaattgt ctaaaaattt tggtaaaagt ttgcagaaaa ctgtcagaaa atgttaattt
     7261 cttacgtgtt tccttgcttg ggacactaga acagcaccga ataagacaaa aagtacaaaa
     7321 cacagcttca tgttggaatt tatgccagaa aaatagctgt aactcggctt ttatagaata
     7381 gaattggttc ctggtgatgc tggagttcta ttaaacatga gaacaggagt atgaaccgtg
     7441 ggaagtggtg ttttggttgg agaggtgttc tgcacggctc ggcaattgtg gtgcatcagt
     7501 gtccaccaaa catcaagcat aacctggatg ataaggagac aagctttggt gcaaaagttt
     7561 tcaaatttta aagcaatttt taggactctt caaagcatga aactgtttct caaggtttcc
     7621 ttctacttct aactccttct agatgcatca cggcctcaaa atgtaaaacc tcagaaatgg
     7681 taccagaggt tcaaaaatgc aaatatcagg gacctgggct agatcttctc gtttcgaggg
     7741 ctgggaaact gcccttccgt gtgaaagggg caattgtcag gcctggagca tgatattgat
     7801 cggttttgtg agaagaccaa caaggtagct aataaatcga tcctatattg tattaacaca
     7861 tgtatttgaa tcagatcttt catttctcat tgactccctc aaatttccag caagaatgga
     7921 tgctacaatt gtaagtttaa ccacaaatca tttttaatta tacctgaatt tcttttcaga
     7981 ccaaaaccag aaaagtcgta ttcatatagt gttgttcaag agattgtcag ttgtatgtgc
     8041 ctaacaaaaa aaaatctatg gcattctaaa tttacaattt cagcatagtc gctcgccatg
     8101 tgtcaccact ctttcgccaa accatcgatt tgatggatcc aggcattgag aaaattcatt
     8161 tactcagttc tgccagtccg tagtgtagtt tttaccggat atagagaatt gaatctagaa
     8221 aacctggctg cctggcaaga agtcactggt agtaacaggc tgatgagggc agccttcggt
     8281 gagctatccg acgtgatggg catgccgaaa cttagactaa gaaagttcag ttttgataac
     8341 acatatggac tggttgggtt atctgggaaa gagaatatgc aatctgtgga tgactgcttc
     8401 aaggctatga acttaccaca tgcgaaggtg cttgaagtga ctggaatcga ctttgtggac
     8461 aatatttcga tggtgaagta ctttcgccca ggttatctcg agaaaatccg catgtattct
     8521 tgttattgct cagctagcca ggagatgagt gaagaaattg caaatctcga gcagtggaag
     8581 caagcgaaac attttctgtg cattggtggt agaatgaaca ttcctattca aagatttttc
     8641 cactttgacc agttctccat cggtcttcag actctatcaa tcgatgatgc tgttcagata
     8701 agagatgtga gttggtagtt aaagtagcgt tttgtaatgt attctatatt ccagattctc
     8761 ctcagatcgc ccagcttcga gtcagcaact atcgatgttc aactacttga aaaagaagcc
     8821 attgagaacg tctttacgcc gaatcaccta ttaatttata gaggaagaga gttcagaccg
     8881 gtgggaactg attccagttt tgtgatcaat ataacaaaaa acaagcttga aatccacaag
     8941 cacaagggag ctaatccacc acgtcgtaga gacgaacggc caatccacga gcgtatacgt
     9001 caaaggcaag atggatggtg aagtttcaat ggttgctcta atacgtttac atgtctatca
     9061 aacctttttc tgttacttaa gtaaatcgtt gtttcatatt taaccagtct tacctacggc
     9121 tggcatatcg gacgtccggc gtcagaacct tgtgcgtctg gttttagatg tttgaaaatt
     9181 ttggaaaatt attaaagtct attgataacc tgtcaaaacc ggacgaccgg acgccaaata
     9241 tgacagccct agtcttacca gtataatatt tttataaacc taaaacaggt atgaatatct
     9301 cttttttctg cgcagcgctg ggatgcttct gtttcagaaa aatcacatgg tataaaaaat
     9361 ttccaaatta cagtagtcct accgtaatcc tgcattatcc cactactgta tgtaccttga
     9421 cattattttt cctcgcccaa ctcccagccc aatatatctc ttcaacagct aagaactaca
     9481 tttttcctaa actacagtaa accaacagta tccctacagt accacattaa ttataaatta
     9541 aaacattatt tttcataaga aatcgtgaga
//
