LOCUS       sequence-274716-        9573 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses mScarlet tagged hrp-1HsLCWT .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9573)
  AUTHORS   Ryan VH, Perdikari TM, Naik MT, Saueressig CF, Lins J, Dignon GL, 
            Mittal J, Hart AC, Fawzi NL
  TITLE     Tyrosine phosphorylation regulates hnRNPA2 granule protein 
            partitioning and reduces neurodegeneration.
  JOURNAL   EMBO J. 2021 Feb 1;40(3):e105001. doi: 10.15252/embj.2020105001. 
            Epub 2020 Dec 22.
  PUBMED    33349959
REFERENCE   2  (bases 1 to 9573)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J."; 
            date: "2021-02-1"; volume: "40(3):e105001. doi"; pages: " 
            10.15252/embj.2020105001. Epub 2020 Dec 22"
COMMENT     Sequence Label: pHA845 - hrp-1p::hrp-1HsLCWTmScarlet
FEATURES             Location/Qualifiers
     source          1..9573
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     72..94
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     86..103
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     87..103
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     113..132
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        113..131
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             4418..5113
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016)"
                     /label=mScarlet
                     /translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYYKQSFPEGFKWERVMNFEDGG
                     AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
                     MALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH
                     STGGMDELYK"
     primer_bind     complement(4551..4569)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4819..4838
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(6826..6842)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6827..6843)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(6872..6892)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(6872..6890)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6911..6927)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6911..6927)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6924..6946)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6935..6951
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6959..6965,6966..6983,6984..6989))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7004..7025
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7142..7159)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7313..7901)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7393..7412)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8072..8863,8864..8932))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8695..8714
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8933..9037)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      9064..9519
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9151..9170)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9361..9382
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac
       61 gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgagcgcg cgtaatacga
      121 ctcactatag ggcgaattgg agctccaccg cggtggcggc cgcttcaatt cttcggagca
      181 ggcgtaggag ctgagcccat cttcaatgcc tccgattttc caaaattaaa cactttttcc
      241 atgaattctt ccattaattt ggcaattgag ccaaatgaaa agccagaaga gccagatgga
      301 gccggtgttg gagttggagc agacagagtt gaacggagtt ctggagcagc tggggttggg
      361 gtcggagcag caagagttga acgtggttca gggtcggcga ggaactgaaa atctaggttt
      421 tttaggaaaa attcgcctaa aaacatgccg aaaaaatttc cgaattttag acaattctgg
      481 aaaaaattgt agtgaaattg tctaaaaatt ttggtaaaag tttgcagaaa actgtcagaa
      541 aatgttaatt tcttacgtgt ttccttgctt gggacactag aacagcaccg aataagacaa
      601 aaagtacaaa acacagcttc atgttggaat ttatgccaga aaaatagctg taactcggct
      661 tttatagaat agaattggtt cctggtgatg ctggagttct attaaacatg agaacaggag
      721 tatgaaccgt gggaagtggt gttttggttg gagaggtgtt ctgcacggct cggcaattgt
      781 ggtgcatcag tgtccaccaa acatcaagca taacctggat gataaggaga caagctttgg
      841 tgcaaaagtt ttcaaatttt aaagcaattt ttaggactct tcaaagcatg aaactgtttc
      901 tcaaggtttc cttctacttc taactccttc tagatgcatc acggcctcaa aatgtaaaac
      961 ctcagaaatg gtaccagagg ttcaaaaatg caaatatcag ggacctgggc tagatcttct
     1021 cgtttcgagg gctgggaaac tgcccttccg tgtgaaaggg gcaattgtca ggcctggagc
     1081 atgatattga tcggttttgt gagaagacca acaaggtagc taataaatcg atcctatatt
     1141 gtattaacac atgtatttga atcagatctt tcatttctca ttgactccct caaatttcca
     1201 gcaagaatgg atgctacaat tgtaagttta accacaaatc atttttaatt atacctgaat
     1261 ttcttttcag accaaaacca gaaaagtcgt attcatatag tgttgttcaa gagattgtca
     1321 gttgtatgtg cctaacaaaa aaaaatctat ggcattctaa atttacaatt tcagcatagt
     1381 cgctcgccat gtgtcaccac tctttcgcca aaccatcgat ttgatggatc caggcattga
     1441 gaaaattcat ttactcagtt ctgccagtcc gtagtgtagt ttttaccgga tatagagaat
     1501 tgaatctaga aaacctggct gcctggcaag aagtcactgg tagtaacagg ctgatgaggg
     1561 cagccttcgg tgagctatcc gacgtgatgg gcatgccgaa acttagacta agaaagttca
     1621 gttttgataa cacatatgga ctggttgggt tatctgggaa agagaatatg caatctgtgg
     1681 atgactgctt caaggctatg aacttaccac atgcgaaggt gcttgaagtg actggaatcg
     1741 actttgtgga caatatttcg atggtgaagt actttcgccc aggttatctc gagaaaatcc
     1801 gcatgtattc ttgttattgc tcagctagcc aggagatgag tgaagaaatt gcaaatctcg
     1861 agcagtggaa gcaagcgaaa cattttctgt gcattggtgg tagaatgaac attcctattc
     1921 aaagattttt ccactttgac cagttctcca tcggtcttca gactctatca atcgatgatg
     1981 ctgttcagat aagagatgtg agttggtagt taaagtagcg ttttgtaatg tattctatat
     2041 tccagattct cctcagatcg cccagcttcg agtcagcaac tatcgatgtt caactacttg
     2101 aaaaagaagc cattgagaac gtctttacgc cgaatcacct attaatttat agaggaagag
     2161 agttcagacc ggtgggaact gattccagtt ttgtgatcaa tataacaaaa aacaagcttg
     2221 aaatccacaa gcacaaggga gctaatccac cacgtcgtag agacgaacgg ccaatccacg
     2281 agcgtatacg tcaaaggcaa gatggatggt gaagtttcaa tggttgctct aatacgttta
     2341 catgtctatc aaaccttttt ctgttactta agtaaatcgt tgtttcatat ttaaccagtc
     2401 ttacctacgg ctggcatatc ggacgtccgg cgtcagaacc ttgtgcgtct ggttttagat
     2461 gtttgaaaat tttggaaaat tattaaagtc tattgataac ctgtcaaaac cggacgaccg
     2521 gacgccaaat atgacagccc tagtcttacc agtataatat ttttataaac ctaaaacagg
     2581 tatgaatatc tcttttttct gcgcagcgct gggatgcttc tgtttcagaa aaatcacatg
     2641 gtataaaaaa tttccaaatt acagtagtcc taccgtaatc ctgcattatc ccactactgt
     2701 atgtaccttg acattatttt tcctcgccca actcccagcc caatatatct cttcaacagc
     2761 taagaactac atttttccta aactacagta aaccaacagt atccctacag taccacatta
     2821 attataaatt aaaacattat ttttcataag aaatcgtgag aatttcgaga aaaataaagg
     2881 attcaaagat tatttgaacg tttttttcgt gaaaaaatgt caaagaaatc aaaatgttca
     2941 tcttccttgt gaaaaatccg aaaagccatt ataaactagt tgattttcat aaaaatttgt
     3001 agaaatattt ttcttaaaat ttgtagaaga attttaacgt tttttaaaca aaaattccaa
     3061 aattggaatt tggtattgaa aatacttttt aaaagttatt tttttaattt gtaaaaattt
     3121 aactaaattc aaaaaataaa gtttccctcc tttttttctt aaaggcacac acatttatcg
     3181 actcgggtct cgccgcgaac agacgcgacg cgacgcggtt aggaatgcgg cgcgtcgcgt
     3241 gtaacccttt tctctgtggt tttcgagagc attctctctc ggttttcgtc acaaaaaggt
     3301 atgttttaca ggttttccgc gcactgtttt cgcgtaattc ttcattttaa ttcagataat
     3361 ctaattgttg tgtagtttta gctcgaattt atccgtaatc tttgattaaa tacgaataaa
     3421 actcgctaaa atagtcttat ttctcagata tttatcgatt tgttattttc cacgaatagt
     3481 ctactcgtgc atggcgtcta tatttaactt aataatctat ttttagattt atctttactc
     3541 taacttgtac ctatttcagt gatcaacacc tctgtcactc tttgtgagta atagcgttcg
     3601 ataggtagtt acaccgtgaa actagcctgt ctatttccta cttcataagc taagaactac
     3661 ttgttagcac aatccgatcg actactaaga actagatcat gacggacgtg gaaatcaagg
     3721 ctgaggttac gtttgttcac ttgcttttag ggtgtaggcc acctgatttg ggtcccgatc
     3781 atttaattat cgattttatt ttcagaatgg aagcggagat gcctcgctcg agccagagaa
     3841 ccttcgaaag attttcgttg gaggactcac gtcaaacacc accgatgacc tgatgcgaga
     3901 gttctactca caattcggag aaatcactga tatcatcgtc atgagagatc caactaccaa
     3961 gagatcccgc ggattcggat ttgtcacctt ctctggcaaa actgaagtcg atgctgccat
     4021 gaaacaacgc ccgcacatca tcgacggaaa gaccgtggac ccgaagcgtg ccgtgccacg
     4081 tgatgataag aaccgctccg agtcgaatgt ctccaccaaa cgcctgtacg tcagcggagt
     4141 tcgtgaggat cacaccgagg acatgctcac cgagtatttc acaaagtatg gaaccgtcac
     4201 caaatccgag attattctcg acaaagccac ccaaaagcca agaggcttcg gattcgtcac
     4261 cttcgatgat catgactctg tggatcaatg cgttcttcaa aaatcccata tggtcaacgg
     4321 acacagatgt gacgtgcgca aaggactctc caaggacgag atgagcaagg ctcagatgaa
     4381 ccgcgatcgt gaaacccgtg gcggaagatc tcgcgatatg gtgagcaagg gcgaggcagt
     4441 gatcaaggag ttcatgcggt tcaaggtgca catggagggc tccatgaacg gccacgagtt
     4501 cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgaa
     4561 ggtgaccaag ggtggccccc tgcccttctc ctgggacatc ctgtcccctc agttcatgta
     4621 cggctccagg gccttcacca agcaccccgc cgacatcccc gactactata agcagtcctt
     4681 ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg ccgtgaccgt
     4741 gacccaggac acctccctgg aggacggcac cctgatctac aaggtgaagc tccgcggcac
     4801 caacttccct cctgacggcc ccgtaatgca gaagaagaca atgggctggg aagcgtccac
     4861 cgagcggttg taccccgagg acggcgtgct gaagggcgac attaagatgg ccctgcgcct
     4921 gaaggacggc ggccgctacc tggcggactt caagaccacc tacaaggcca agaagcccgt
     4981 gcagatgccc ggcgcctaca acgtcgaccg caagttggac atcacctccc acaacgagga
     5041 ctacaccgtg gtggaacagt acgaacgctc cgagggccgc cactccaccg gcggcatgga
     5101 cgagctgtac aagggacagc gtggcggtta caatggcggt ggtggtggcg gcggcggctg
     5161 gggaggacca gctcaacgcg gcggtccagg agcatatggt ggaccaggag gtggcggcca
     5221 aggaggatat ggcggaggta gttggattct ggcttgtgaa ttctcgattc taatattatt
     5281 aactttcaga ttacggagga ggacgcggtg gttatggtgg tggaggacca ggatatggaa
     5341 atcagggtgg aggatatgga ggaggatatg ataattatgg aggaggaaat tacggatctg
     5401 gaaattacaa tgatttcgga aactacaacc agcagccatc taattatgga ccaatgaagt
     5461 ctggaaattt cggaggatct cgcaatatgg gtggaccata tggaggagga aattatggtc
     5521 caggaggatc tggaggatct ggaggatatg gaggacgttc tcgttactaa attaattcct
     5581 taagcccctc taagtgtcca acgtgtctga gcttcccgtg ctctctcctc tgtatcgatc
     5641 ttctcaacca tttttgctct gctatctcat caaaatcttc tgtacttttt cttgttgcct
     5701 ttctccagaa agtttcgttt attattcaat tttttaccct gtctttttta tataatttat
     5761 ttctaaattc gcacagttga catccattca actagagaat gaatgcagag tttgacacca
     5821 aattggctta attttcaagc tgtttttaac caaaataata aaaaagtctc aactttacaa
     5881 aaatgtttat ctggatctgg acgacagcga gctgagaaat tgatatattt tcagagtaaa
     5941 aactaaaaat gtctggaatt tttggagtca ttgtctcagg gaggacgccg atcgaagtgg
     6001 tgccggtctc cgatacggag ttcacctgcg aaattgtaaa cgctgacgcg ataaaccacg
     6061 tggtggtctt ccttaccgga gctcaaccgt ttccggacgg aattggtggc tctggtgatc
     6121 attttcagaa ttttccgccc aaaaaaaccc aatttatttc agtttacatc agatggccca
     6181 cccaagacgg cggaaattgg cattatttgg gatttatttg caacgagaag cccagcgcga
     6241 tttataaagt tgctcaggtg tgcatttttg atggaaaaat gaaagaaaac caccgaaatt
     6301 tcgatgtttc tctgtcgatt ttttttattt aaaaacttag aaatactctc aatttcgcat
     6361 ttttcaccaa aaaaaagaat ctcaactggt ttttcacata gcttaatttt tgaagaaaaa
     6421 tctcaacaaa acccagtttt aagtttcaaa ttctcaaaaa ttgatctttt ttttccctta
     6481 aaaatccagt ttttagctcc aaattgtcat aattttccag aaaaatttag ttttcgagct
     6541 aacaacatta aaatttcaca gaaaatctga atttcagcct tagagatcca aatttttcgg
     6601 ctgaaaatca caatttttcc atttttcagc tccacaaatc cgacgcttct cactcgtttt
     6661 cccaatttga caatcaaatg caactatatt ccagtggatc agctcaaatt ggaattaatg
     6721 ctgaaagttt atctgatatc actggacggc aggcagctga tgggacacag gctagccagc
     6781 aggtgaagga tcccccgggc tgcaggaatt cgatatcaag cttatcgata ccgtcgacct
     6841 cgaggggggg cccggtaccc agcttttgtt ccctttagtg agggttaatt gcgcgcttgg
     6901 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
     6961 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
     7021 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
     7081 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     7141 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     7201 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     7261 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     7321 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     7381 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     7441 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     7501 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     7561 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     7621 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     7681 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     7741 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     7801 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     7861 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     7921 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     7981 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     8041 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     8101 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     8161 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     8221 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     8281 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     8341 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     8401 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     8461 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     8521 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     8581 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     8641 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     8701 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     8761 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     8821 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     8881 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     8941 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     9001 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     9061 ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     9121 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     9181 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     9241 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
     9301 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     9361 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
     9421 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     9481 ttaacgcgaa ttttaacaaa atattaacgc ttacaatttc cattcgccat tcaggctgcg
     9541 caactgttgg gaagggcgat cggtgcgggc ctc
//
