LOCUS       sequence-274717-        9573 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses mScarlet tagged hrp-1HsLCD290V .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9573)
  AUTHORS   Ryan VH, Perdikari TM, Naik MT, Saueressig CF, Lins J, Dignon GL, 
            Mittal J, Hart AC, Fawzi NL
  TITLE     Tyrosine phosphorylation regulates hnRNPA2 granule protein 
            partitioning and reduces neurodegeneration.
  JOURNAL   EMBO J. 2021 Feb 1;40(3):e105001. doi: 10.15252/embj.2020105001. 
            Epub 2020 Dec 22.
  PUBMED    33349959
REFERENCE   2  (bases 1 to 9573)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J."; 
            date: "2021-02-1"; volume: "40(3):e105001. doi"; pages: " 
            10.15252/embj.2020105001. Epub 2020 Dec 22"
COMMENT     Sequence Label: pHA846 - hrp-1p::hrp-1HsLCD290VmScarlet
FEATURES             Location/Qualifiers
     source          1..9573
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     145..167
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     159..176
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     160..176
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     186..205
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        186..204
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             4491..5186
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016)"
                     /label=mScarlet
                     /translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYYKQSFPEGFKWERVMNFEDGG
                     AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
                     MALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH
                     STGGMDELYK"
     primer_bind     complement(4624..4642)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4892..4911
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(6899..6915)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6900..6916)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(6945..6965)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(6945..6963)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6984..7000)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6984..7000)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6997..7019)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7008..7024
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7032..7038,7039..7056,7057..7062))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7077..7098
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7215..7232)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7386..7974)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7466..7485)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8145..8936,8937..9005))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8768..8787
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9006..9110)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      9137..9565
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9224..9243)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9434..9455
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 atattaacgc ttacaatttc cattcgccat tcaggctgcg caactgttgg gaagggcgat
       61 cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat
      121 taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgagc
      181 gcgcgtaata cgactcacta tagggcgaat tggagctcca ccgcggtggc ggccgcttca
      241 attcttcgga gcaggcgtag gagctgagcc catcttcaat gcctccgatt ttccaaaatt
      301 aaacactttt tccatgaatt cttccattaa tttggcaatt gagccaaatg aaaagccaga
      361 agagccagat ggagccggtg ttggagttgg agcagacaga gttgaacgga gttctggagc
      421 agctggggtt ggggtcggag cagcaagagt tgaacgtggt tcagggtcgg cgaggaactg
      481 aaaatctagg ttttttagga aaaattcgcc taaaaacatg ccgaaaaaat ttccgaattt
      541 tagacaattc tggaaaaaat tgtagtgaaa ttgtctaaaa attttggtaa aagtttgcag
      601 aaaactgtca gaaaatgtta atttcttacg tgtttccttg cttgggacac tagaacagca
      661 ccgaataaga caaaaagtac aaaacacagc ttcatgttgg aatttatgcc agaaaaatag
      721 ctgtaactcg gcttttatag aatagaattg gttcctggtg atgctggagt tctattaaac
      781 atgagaacag gagtatgaac cgtgggaagt ggtgttttgg ttggagaggt gttctgcacg
      841 gctcggcaat tgtggtgcat cagtgtccac caaacatcaa gcataacctg gatgataagg
      901 agacaagctt tggtgcaaaa gttttcaaat tttaaagcaa tttttaggac tcttcaaagc
      961 atgaaactgt ttctcaaggt ttccttctac ttctaactcc ttctagatgc atcacggcct
     1021 caaaatgtaa aacctcagaa atggtaccag aggttcaaaa atgcaaatat cagggacctg
     1081 ggctagatct tctcgtttcg agggctggga aactgccctt ccgtgtgaaa ggggcaattg
     1141 tcaggcctgg agcatgatat tgatcggttt tgtgagaaga ccaacaaggt agctaataaa
     1201 tcgatcctat attgtattaa cacatgtatt tgaatcagat ctttcatttc tcattgactc
     1261 cctcaaattt ccagcaagaa tggatgctac aattgtaagt ttaaccacaa atcattttta
     1321 attatacctg aatttctttt cagaccaaaa ccagaaaagt cgtattcata tagtgttgtt
     1381 caagagattg tcagttgtat gtgcctaaca aaaaaaaatc tatggcattc taaatttaca
     1441 atttcagcat agtcgctcgc catgtgtcac cactctttcg ccaaaccatc gatttgatgg
     1501 atccaggcat tgagaaaatt catttactca gttctgccag tccgtagtgt agtttttacc
     1561 ggatatagag aattgaatct agaaaacctg gctgcctggc aagaagtcac tggtagtaac
     1621 aggctgatga gggcagcctt cggtgagcta tccgacgtga tgggcatgcc gaaacttaga
     1681 ctaagaaagt tcagttttga taacacatat ggactggttg ggttatctgg gaaagagaat
     1741 atgcaatctg tggatgactg cttcaaggct atgaacttac cacatgcgaa ggtgcttgaa
     1801 gtgactggaa tcgactttgt ggacaatatt tcgatggtga agtactttcg cccaggttat
     1861 ctcgagaaaa tccgcatgta ttcttgttat tgctcagcta gccaggagat gagtgaagaa
     1921 attgcaaatc tcgagcagtg gaagcaagcg aaacattttc tgtgcattgg tggtagaatg
     1981 aacattccta ttcaaagatt tttccacttt gaccagttct ccatcggtct tcagactcta
     2041 tcaatcgatg atgctgttca gataagagat gtgagttggt agttaaagta gcgttttgta
     2101 atgtattcta tattccagat tctcctcaga tcgcccagct tcgagtcagc aactatcgat
     2161 gttcaactac ttgaaaaaga agccattgag aacgtcttta cgccgaatca cctattaatt
     2221 tatagaggaa gagagttcag accggtggga actgattcca gttttgtgat caatataaca
     2281 aaaaacaagc ttgaaatcca caagcacaag ggagctaatc caccacgtcg tagagacgaa
     2341 cggccaatcc acgagcgtat acgtcaaagg caagatggat ggtgaagttt caatggttgc
     2401 tctaatacgt ttacatgtct atcaaacctt tttctgttac ttaagtaaat cgttgtttca
     2461 tatttaacca gtcttaccta cggctggcat atcggacgtc cggcgtcaga accttgtgcg
     2521 tctggtttta gatgtttgaa aattttggaa aattattaaa gtctattgat aacctgtcaa
     2581 aaccggacga ccggacgcca aatatgacag ccctagtctt accagtataa tatttttata
     2641 aacctaaaac aggtatgaat atctcttttt tctgcgcagc gctgggatgc ttctgtttca
     2701 gaaaaatcac atggtataaa aaatttccaa attacagtag tcctaccgta atcctgcatt
     2761 atcccactac tgtatgtacc ttgacattat ttttcctcgc ccaactccca gcccaatata
     2821 tctcttcaac agctaagaac tacatttttc ctaaactaca gtaaaccaac agtatcccta
     2881 cagtaccaca ttaattataa attaaaacat tatttttcat aagaaatcgt gagaatttcg
     2941 agaaaaataa aggattcaaa gattatttga acgttttttt cgtgaaaaaa tgtcaaagaa
     3001 atcaaaatgt tcatcttcct tgtgaaaaat ccgaaaagcc attataaact agttgatttt
     3061 cataaaaatt tgtagaaata tttttcttaa aatttgtaga agaattttaa cgttttttaa
     3121 acaaaaattc caaaattgga atttggtatt gaaaatactt tttaaaagtt atttttttaa
     3181 tttgtaaaaa tttaactaaa ttcaaaaaat aaagtttccc tccttttttt cttaaaggca
     3241 cacacattta tcgactcggg tctcgccgcg aacagacgcg acgcgacgcg gttaggaatg
     3301 cggcgcgtcg cgtgtaaccc ttttctctgt ggttttcgag agcattctct ctcggttttc
     3361 gtcacaaaaa ggtatgtttt acaggttttc cgcgcactgt tttcgcgtaa ttcttcattt
     3421 taattcagat aatctaattg ttgtgtagtt ttagctcgaa tttatccgta atctttgatt
     3481 aaatacgaat aaaactcgct aaaatagtct tatttctcag atatttatcg atttgttatt
     3541 ttccacgaat agtctactcg tgcatggcgt ctatatttaa cttaataatc tatttttaga
     3601 tttatcttta ctctaacttg tacctatttc agtgatcaac acctctgtca ctctttgtga
     3661 gtaatagcgt tcgataggta gttacaccgt gaaactagcc tgtctatttc ctacttcata
     3721 agctaagaac tacttgttag cacaatccga tcgactacta agaactagat catgacggac
     3781 gtggaaatca aggctgaggt tacgtttgtt cacttgcttt tagggtgtag gccacctgat
     3841 ttgggtcccg atcatttaat tatcgatttt attttcagaa tggaagcgga gatgcctcgc
     3901 tcgagccaga gaaccttcga aagattttcg ttggaggact cacgtcaaac accaccgatg
     3961 acctgatgcg agagttctac tcacaattcg gagaaatcac tgatatcatc gtcatgagag
     4021 atccaactac caagagatcc cgcggattcg gatttgtcac cttctctggc aaaactgaag
     4081 tcgatgctgc catgaaacaa cgcccgcaca tcatcgacgg aaagaccgtg gacccgaagc
     4141 gtgccgtgcc acgtgatgat aagaaccgct ccgagtcgaa tgtctccacc aaacgcctgt
     4201 acgtcagcgg agttcgtgag gatcacaccg aggacatgct caccgagtat ttcacaaagt
     4261 atggaaccgt caccaaatcc gagattattc tcgacaaagc cacccaaaag ccaagaggct
     4321 tcggattcgt caccttcgat gatcatgact ctgtggatca atgcgttctt caaaaatccc
     4381 atatggtcaa cggacacaga tgtgacgtgc gcaaaggact ctccaaggac gagatgagca
     4441 aggctcagat gaaccgcgat cgtgaaaccc gtggcggaag atctcgcgat atggtgagca
     4501 agggcgaggc agtgatcaag gagttcatgc ggttcaaggt gcacatggag ggctccatga
     4561 acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga
     4621 ccgccaagct gaaggtgacc aagggtggcc ccctgccctt ctcctgggac atcctgtccc
     4681 ctcagttcat gtacggctcc agggccttca ccaagcaccc cgccgacatc cccgactact
     4741 ataagcagtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg
     4801 gcgccgtgac cgtgacccag gacacctccc tggaggacgg caccctgatc tacaaggtga
     4861 agctccgcgg caccaacttc cctcctgacg gccccgtaat gcagaagaag acaatgggct
     4921 gggaagcgtc caccgagcgg ttgtaccccg aggacggcgt gctgaagggc gacattaaga
     4981 tggccctgcg cctgaaggac ggcggccgct acctggcgga cttcaagacc acctacaagg
     5041 ccaagaagcc cgtgcagatg cccggcgcct acaacgtcga ccgcaagttg gacatcacct
     5101 cccacaacga ggactacacc gtggtggaac agtacgaacg ctccgagggc cgccactcca
     5161 ccggcggcat ggacgagctg tacaagggac agcgtggcgg ttacaatggc ggtggtggtg
     5221 gcggcggcgg ctggggagga ccagctcaac gcggcggtcc aggagcatat ggtggaccag
     5281 gaggtggcgg ccaaggagga tatggcggag gtagttggat tctggcttgt gaattctcga
     5341 ttctaatatt attaactttc agattacgga ggaggacgcg gtggttatgg tggtggagga
     5401 ccaggatatg gaaatcaggg tggaggatat ggaggaggat atgataatta tggaggagga
     5461 aattacggat ctggaaatta caatgttttc ggaaactaca accagcagcc atctaattat
     5521 ggaccaatga agtctggaaa tttcggagga tctcgcaata tgggtggacc atatggagga
     5581 ggaaattatg gtccaggagg atctggagga tctggaggat atggaggacg ttctcgttac
     5641 taaattaatt ccttaagccc ctctaagtgt ccaacgtgtc tgagcttccc gtgctctctc
     5701 ctctgtatcg atcttctcaa ccatttttgc tctgctatct catcaaaatc ttctgtactt
     5761 tttcttgttg cctttctcca gaaagtttcg tttattattc aattttttac cctgtctttt
     5821 ttatataatt tatttctaaa ttcgcacagt tgacatccat tcaactagag aatgaatgca
     5881 gagtttgaca ccaaattggc ttaattttca agctgttttt aaccaaaata ataaaaaagt
     5941 ctcaacttta caaaaatgtt tatctggatc tggacgacag cgagctgaga aattgatata
     6001 ttttcagagt aaaaactaaa aatgtctgga atttttggag tcattgtctc agggaggacg
     6061 ccgatcgaag tggtgccggt ctccgatacg gagttcacct gcgaaattgt aaacgctgac
     6121 gcgataaacc acgtggtggt cttccttacc ggagctcaac cgtttccgga cggaattggt
     6181 ggctctggtg atcattttca gaattttccg cccaaaaaaa cccaatttat ttcagtttac
     6241 atcagatggc ccacccaaga cggcggaaat tggcattatt tgggatttat ttgcaacgag
     6301 aagcccagcg cgatttataa agttgctcag gtgtgcattt ttgatggaaa aatgaaagaa
     6361 aaccaccgaa atttcgatgt ttctctgtcg atttttttta tttaaaaact tagaaatact
     6421 ctcaatttcg catttttcac caaaaaaaag aatctcaact ggtttttcac atagcttaat
     6481 ttttgaagaa aaatctcaac aaaacccagt tttaagtttc aaattctcaa aaattgatct
     6541 tttttttccc ttaaaaatcc agtttttagc tccaaattgt cataattttc cagaaaaatt
     6601 tagttttcga gctaacaaca ttaaaatttc acagaaaatc tgaatttcag ccttagagat
     6661 ccaaattttt cggctgaaaa tcacaatttt tccatttttc agctccacaa atccgacgct
     6721 tctcactcgt tttcccaatt tgacaatcaa atgcaactat attccagtgg atcagctcaa
     6781 attggaatta atgctgaaag tttatctgat atcactggac ggcaggcagc tgatgggaca
     6841 caggctagcc agcaggtgaa ggatcccccg ggctgcagga attcgatatc aagcttatcg
     6901 ataccgtcga cctcgagggg gggcccggta cccagctttt gttcccttta gtgagggtta
     6961 attgcgcgct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
     7021 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     7081 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     7141 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     7201 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     7261 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     7321 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     7381 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     7441 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     7501 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     7561 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     7621 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     7681 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     7741 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     7801 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     7861 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     7921 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     7981 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     8041 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     8101 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     8161 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     8221 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     8281 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     8341 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     8401 cgggaagcta gagtaagtag ttcaccagtt aatagtttgc gcaacgttgt tgccattgct
     8461 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     8521 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     8581 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     8641 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     8701 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     8761 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     8821 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     8881 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     8941 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     9001 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     9061 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     9121 cgaaaagtgc cacctgacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     9181 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     9241 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     9301 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
     9361 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     9421 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
     9481 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     9541 atttaacaaa aatttaacgc gaattttaac aaa
//
