LOCUS       sequence-274718-        6123 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express activated Fyn kinase in osm-10 neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6123)
  AUTHORS   Ryan VH, Perdikari TM, Naik MT, Saueressig CF, Lins J, Dignon GL, 
            Mittal J, Hart AC, Fawzi NL
  TITLE     Tyrosine phosphorylation regulates hnRNPA2 granule protein 
            partitioning and reduces neurodegeneration.
  JOURNAL   EMBO J. 2021 Feb 1;40(3):e105001. doi: 10.15252/embj.2020105001. 
            Epub 2020 Dec 22.
  PUBMED    33349959
REFERENCE   2  (bases 1 to 6123)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J."; 
            date: "2021-02-1"; volume: "40(3):e105001. doi"; pages: " 
            10.15252/embj.2020105001. Epub 2020 Dec 22"
COMMENT     Sequence Label: pHA850 - osm-10p::FynY531F
FEATURES             Location/Qualifiers
     source          1..6123
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     17..39
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     31..48
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     32..48
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     58..77
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        58..76
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3367..3387)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(3367..3385)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3406..3422)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3406..3422)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3419..3441)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3430..3446
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3454..3460,3461..3478,3479..3484))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3499..3520
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3637..3654)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3808..4396)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3888..3907)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4567..5358,5359..5427))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5190..5209
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5428..5532)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      5559..6014
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5646..5665)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5856..5877
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga gcgcgcgtaa
       61 tacgactcac tatagggcga attggagctc caccgcggtg gcggccgctc tagaactagt
      121 ctgcagcttg acaccgactg gcacacacaa aatattaaac tttcagggtc taatccatca
      181 cgaaatgaac gaccacaacc tcaatgcaaa atgtcattta tgccagaaat ctttcgagaa
      241 taagaagaca ttccgtgaac actgtgaatt ggatcacaca ccggaagtgg tatgacagta
      301 ttcattaaca gattcaattt gaaatattaa ttttaggtta gatgtgtatt ctgcaaaagc
      361 tcattcgatc agccactgga aatgaacgat gaacaatggg cacacttttt cgctcatatt
      421 tatggggtta gtaattcacc attcgaatct agaagtcgat tctcctgact gacattttta
      481 aaaccatttt ttccaggaaa tcctctactc ccgccttgcc gactacgagg atcgtgcttt
      541 gaacactgca tgaattatat atttgattcg atggttagct taataaagaa tttttctgtt
      601 gtacttcaac ctttttctga aataggaaat aatcacaagt tgtctgaaaa atgtgatcag
      661 ctatccagat caaacagtga aaatgttgtc tgcaatgatt catagctgaa catctgaact
      721 cttaaatttt cagcgaatca tgatttgcat tgtgatatat tattcttatg gttgcaaaag
      781 aaaaaagatt acgaaaatat cttgtgacag agtaagaaac cattgaaatt taattaatca
      841 ccgtggagac aggtgtgcac tctgctctta ttatattatc aaaacgagaa acgcgtgagc
      901 aagaagtggg cgtgacttgg tagaaaggta cttaatcgag atgttgagcc aactttcgag
      961 attattctaa aagtttccat atcaataaac ttttttggaa atttcatctt gcaagatgtc
     1021 atgggctgtg tgcaatgtaa ggataaagaa gcaacaaaac tgacggagga gagggacggc
     1081 agcctgaacc agagctctgg gtaccgctat ggcacagacc ccacccctca gcactacccc
     1141 agcttcggtg tgacctccat ccccaactac aacaacttcc acgcagccgg gggccaagga
     1201 ctcaccgtct ttggaggtgt gaactcttcg tctcatacgg ggaccttgcg tacgagagga
     1261 ggaacaggag tgacactctt tgtggccctt tatgactatg aagcacggac agaagatgac
     1321 ctgagttttc acaaaggaga aaaatttcaa atattgaaca gctcggaagg agattggtgg
     1381 gaagcccgct ccttgacaac tggagagaca ggttacattc ccagcaatta tgtggctcca
     1441 gttgactcta tccaggcaga agagtggtac tttggaaaac ttggccgaaa agatgctgag
     1501 cgacagctat tgtcctttgg aaacccaaga ggtacctttc ttatccgcga gagtgaaacc
     1561 accaaaggtg cctattcact ttctatccgt gattgggatg atatgaaagg agaccatgtc
     1621 aaacattata aaattcgcaa acttgacaat ggtggatact acattaccac ccgggcccag
     1681 tttgaaacac ttcagcagct tgtacaacat tactcagaga gagctgcagg tctctgctgc
     1741 cgcctagtag ttccctgtca caaagggatg ccaaggctta ccgatctgtc tgtcaaaacc
     1801 aaagatgtct gggaaatccc tcgagaatcc ctgcagttga tcaagagact gggaaatggg
     1861 cagtttgggg aagtatggat gggtacctgg aatggaaaca caaaagtagc cataaagact
     1921 cttaaaccag gcacaatgtc ccccgaatca ttccttgagg aagcgcagat catgaagaag
     1981 ctgaagcacg acaagctggt ccagctctat gcagtggtgt ctgaggagcc catctacatc
     2041 gtcaccgagt atatgaacaa aggaagttta ctggatttct taaaagatgg agaaggaaga
     2101 gctctgaaat taccaaatct tgtggacatg gcagcacagg tggctgcagg aatggcttac
     2161 atcgagcgca tgaattatat ccatagagat ctgcgatcag caaacattct agtggggaat
     2221 ggactcatat gcaagattgc tgacttcgga ttggcccgat tgatagaaga caatgagtac
     2281 acagcaagac aaggtgcaaa gttccccatc aagtggacgg cccccgaggc agccctgtac
     2341 gggaggttca caatcaagtc tgacgtgtgg tcttttggaa tcttactcac agagctggtc
     2401 accaaaggaa gagtgccata cccaggcatg aacaaccggg aggtgctgga gcaggtggag
     2461 cgaggctaca ggatgccctg cccgcaggac tgccccatct ctctgcatga gctcatgatc
     2521 cactgctgga aaaaggaccc tgaagaacgc cccacttttg agtacttgca gagcttcctg
     2581 gaagactact ttaccgcgac agagccccag ttccaacctg gtgaaaacct gtaagtccaa
     2641 ttactcttca acatccctac atgctctttc tccctgtgct cccaccccct atttttgtta
     2701 ttatcaaaaa acttctctta atttctttgt tttttagctt cttttaagtc acctctaaca
     2761 atgaaattgt gtagattcaa aaatagaatt aattcgtaat aaaaagtcga aaaaaattgt
     2821 gctccctccc cccattaata ataattctat cccaaaatct acacaatgtt ctgtgtacac
     2881 ttcttatgtt ttttacttct gataaatttt tttgaaacat catagaaaaa accgcacaca
     2941 aaatacctta tcatatgtta cgtttcagtt tatgaccgca atttttattt cttcgcacgt
     3001 ctgggcctct catgacgtca aatcatgctc atcgtgaaaa agttttggag tatttttgga
     3061 atttttcaat caagtgaaag tttatgaaat taattttcct gcttttgctt tttggggttt
     3121 cccctattgt ttgtcaagat ttcgaggacg gcgtttttct tgctaaaatc acaagtattg
     3181 atgagcacga tgcaagaaag atcggaagaa ggtttgggtt tgaggctcag tggaaggtga
     3241 gtagaagttg ataatttgaa agtggagtag tgtctatggg gtttttgcct taaatgacag
     3301 aatacattcc caatatacca aacataactg tttctcgagg gggggcccgg tacccagctt
     3361 ttgttccctt tagtgagggt taattgcgcg cttggcgtaa tcatggtcat agctgtttcc
     3421 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     3481 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     3541 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     3601 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     3661 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     3721 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     3781 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     3841 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     3901 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     3961 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     4021 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     4081 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     4141 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     4201 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     4261 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     4321 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     4381 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     4441 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     4501 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     4561 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     4621 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     4681 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     4741 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     4801 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     4861 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     4921 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     4981 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     5041 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     5101 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     5161 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     5221 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     5281 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     5341 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     5401 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     5461 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     5521 aggggttccg cgcacatttc cccgaaaagt gccacctgac gcgccctgta gcggcgcatt
     5581 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
     5641 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     5701 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     5761 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     5821 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     5881 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc
     5941 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt
     6001 aacgcttaca atttccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg
     6061 cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt
     6121 tgg
//
