LOCUS       sequence-27472-e         709 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 709)
  AUTHORS   Muona M, Aranko AS, Iwai H
  TITLE     Segmental isotopic labelling of a multidomain protein by protein 
            ligation by protein trans-splicing.
  JOURNAL   Chembiochem. 2008 Dec 15. 9(18):2958-61.
  PUBMED    19031436
REFERENCE   2  (bases 1 to 709)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Chembiochem. 2008 Dec 15. 9(18):2958-61."
COMMENT     Sequence Label: pMMBAD16
FEATURES             Location/Qualifiers
     source          1..709
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             64..86
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(469..486)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      639..686
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 tgacgctttt tatcgcaact ctctactgtt tctccatacc cgtttttttg ggctagaaat
       61 aattttgttt aactttaaga aggagatata catatggacc ataattttgc actcaaaaat
      121 ggcttcatag cttctaattg tttcaatggt accctgggtg ggccggagcc tgggccctat
      181 gcccaaccca gcgtcaacac tccgctccct aacctccaga atgggcccat ttatgccagg
      241 gttatccaga agcgagtccc taatgcctac gacaagacag ccttggcttt ggaggtcggt
      301 gagctggtaa aggttacgaa gattaatgtg agtggtcagt gggaagggga gtgtaatggc
      361 aaacgaggtc acttcccatt cacacatgtc cgtctgctgg atcaacagaa tcccgatgag
      421 gacttcagct aagcttggct gttttggcgg atgagagaag attttcagcc tgatacagat
      481 taaatcagaa cgcagaagcg gtctgataaa acagaatttg cctggcggca gtagcgcggt
      541 ggtcccacct gaccccatgc cgaactcaga agtgaaacgc cgtagcgccg atggtagtgt
      601 ggggtctccc catgcgagag tagggaactg ccaggcatca aataaaacga aaaggctcag
      661 tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctc
//
